• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.23-27
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• 2008

Traditionally mungbean(Vigna radiata L.) sprouts has been eaten soon after washing. The study was carried out to measure the effect of washing before packaging on morphological characters and color of mungbean sprouts. The seeds of cv. Zhong Lu 1 were soaked in 50 ppm BA solution immediately before 4 hour aeration and then cultured for 6 days. The sprouts were washed immediately before packaged with PE envelops or not, and then stored 5 days at $8^{\circ}C$. Their morphological characters, fresh and dry weights, and colors were measured everyday. Compared to washed, nonwashed sprouts had more lateral roots although the two sprouts did nearly same in hypocotyl and root lengths, hypocotyl diameter, fresh and dry weights. Non-washed sprouts, moreover, showed higher brightness in hypocotyl and root and cutting resistance in hypocotyl although there were not significant differences in color a and b, meaning that the latter ones were more rapidly changed during their storage. In non-washed sprouts, number of lateral roots, hypocotyl length and diameter, total fresh and dry weights were nearly same up to 3 days and afterward were declined. Brightness and color b of hypocotyl were decreased with increased storage period although cutting resistance of hypocotyl was since 3 days after storage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.28-33
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• 2008

Traditionally mungbean (Vigna radiata L.) sprouts has been stored after dehydration to decrease their decay. The study was done to determine the effect of watering for final 12 hours of harvest day on morphological characters and color of mungbean sprouts. The seeds of cv. Zhong Lu 1 were soaked in 50 ppm BA solution immediately before 4 hour aeration and then cultured for 6 days, when water-supplied for final 12 hours or not. After packaged with PE envelops, the sprouts stored 5 days at $8^{\circ}C$, and their morphological characters, fresh and dry weights, and colors were measured everyday. Compared to non-water supplied, water supplied sprouts had thicker hypocotyl diameter in middle part, and higher total fresh weight due to increment of cotyledon and hypocotyl ones although the two sprouts did nearly same in other characters. Only nonwater supplied sprouts showed higher color b value in hypocotyl than water supplied ones although there were not significant differences between them in other color-related characters, meaning that the latter ones were more rapidly decayed during their storage. In non-water supplied sprouts, number of lateral roots, hypocotyl and root lengths, total fresh weight, brightness of hypocotyl and root was decreased since 3 days after storage but cutting resistance of hypocotyl was done since 5 days.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.94-101
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• 2004

Commercial value of soybean sprouts should be affected by their morphology including the lateral roots. This study was carried out to determine the effect of benzyladenopurine (BA) concentration on growth and morphology of soybean sprouts in order to compare them with the selling products collected from the markets. Four cultivars (cv. Eunhakong, Pungsannamulkong, Sowonkong, and Junjery) were cultured for 6 days after imbibed for 5 hours into different BA solutions (0, 1,2,4, and 8 ppm). On the 6th day, hypocotyl length was measured to calculate the composition rate of ＞ 7 cm (A), 4 to 7 cm (B), ＜ 4 cm (C) and non-germination (D), and the ratio of hypocotyl to root length (H/R ratio) on the base of hypocotyl length as well as lateral root, hypocotyl diameter, and fraction fresh and dry weights. The composition rates classified with hypocotyl length showed big differences between 4 cultivars in class A and C; Class A, in which cv. Sowonkong and Junjery had higher rates than cv. Eunhakong and Pungsannamulkong, was decreased with BA concentration of higher than 4 ppm, but class C showed the reverse result to class A. Any lateral root was not formed in higher than 4 ppm BA solutions although fewer in cv. Pungsannamulkong and Junjery than in the two other cultivars. Hypocotyl and root lengths were decreased with increased BA concentrations, and in all BA concentration, hypocotyl length of cv. Junjery was the longest. Hypocotyl diameter, hypocotyl and total fresh weights were thicker and heavier in 2 to 4 ppm BA concentrations than in the other ones, although in case of BA application, cv, Eunhakong and Sowonkong were heavier. The H/R ratios were increased with increased BA concentration. The ratios of nearly all the selling products ranged from 1 to 2 ppm when compared to them from our experiment.

• Korean Journal of Weed Science
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• v.7 no.1
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• pp.78-83
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• 1987

This study was attempted to evaluate the combining effect of HBR (homobrassinolide) with the known growth regulators such as GA (gibberellic acid), BA(6-benzyl aminopurine), IAA (indole-3-acetic acid), B-9 (N-dimethylamino succinamic acid) and CCC (2-chloroethyl-trimethylammonium chloride) on the growth of radish hypocotyl. A single application of HBR increased hypocotyl growth as its rates increased from 0.1 to 1.0 ppm, showing a maximum increase at 1.0 ppm. GA and BA had no direct effects on hyopcotyl growth, but IAA showed some effect as its concentration increased. However, the mixed application of HBR with GA, BA and IAA increased the length of radish hypocotyl as the concentration of HBR became higher. The mixture of HBR with GA and BA showed antagonistic reaction on radish hypocotyl growth, but synergistic effect was shown in the higher rate mixture of HBR with IAA in the range of HBR at 0.03 to 0.30 ppm with IAA at 3.0 to 10.0 ppm, but antagonistic or additive response at the mixture of low rates. An increased growth of hypocotyl by HBR was ified by CCC, showing the strong antagonistic reaction, but B-9 was not able to ify HBR's effect on hypocotyl growth.

• Horticultural Science & Technology
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• v.16 no.3
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• pp.344-346
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• 1998

Early emergence of watermelon seeds was markedly enhanced by SMP treatment though the final emergence percentage was not significantly influenced. Mixing ratios of 10:1:5 and 10:1:6 (seed : Micro-Cel E : water by weight) were found to be most effective in promoting the early emergence of watermelon seeds primed for 72 hrs at $25^{\circ}C$. Even though the hypocotyl elongation was slightly promoted by SMP treatment, the hypocotyl diameter remained uninfluenced. Among the cultivars tested, 'House-Ilpum' and 'Surowang' showed the best response to the SMP treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.487-490
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• 2004

Methods for culturing bean sprouts could be divided into two main groups of overspraying and underwatering according to water supplying. The study was done to measure the effect of water supplying method on growth of mungbean sprouts and temperatures inside their culture boxes. The morphological characters, fresh and dry weights were measured on the 6th day after culturing, but temperatures of the boxes were recorded daily by dataloggers. Lateral roots was not formed in the under-watering method (UM) while partially done in the over-spraying method (OM). OM had longer hypocotyl but UM had longer root compared to the other. Two water supplying methods, however, had nearly similar total length adding hypocotyl and root lengths, diameters of middle and upper parts of hypocotyl. OM showed more total fresh weight than UM mainly resulting from increment of hypocotyl fresh weight. The fresh weight increment in OM was caused by relatively higher temperature of culture box compared to UM.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.482-486
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• 2004

Benzyladenopurine (BA), a synthetic chemical commonly used for bean sprout culture, should be minimized for wellbeing foods or not be applied. The study was done to check the effect of different BA concentrations treated during 5 hour imbibition on growth and morphological characters of mungbean sprouts. The mungbean seeds of 3 cultivars (Keumseongnogdu, Owoolnogdu, and Zhong Lu 1) were imbibed for 5 hours in the solutions with different BA concentrations (0, 25, 50, 75, and 100 ppm) before 3 hour aeration. On the 6th day after culture, the mungbean sprouts were classified by 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm, < 4cm, and non-germination, and their morphological characters, fresh and dry weights were measured. Regardless of cultivars the composition rate of hypocotyls of longer than 7cm was decreased with increased BA concentration over 50 ppm while the reverse result was true in the rate of shorter than 4cm. In the rate of 4 to 7cm. cv. Owoolnogdu showed the highest rate in its 50 ppm concentration although cv. Keumseongnogdu and Zhong Lu 1 showed similar result to the above two rates. Formation rate and its number of lateral roots were largely changed around 50 ppm concentration but the roots was not formed in over its 75 ppm concentration. Hypocotyl and root lengths of all the cultivars were shortened with increased BA concentration. In the diameter of middle part of hypocotyl, 3 cultivars showed nearly the same responses as the rate of 4 to 7cm hypocotyls. Hypocotyl and total fresh weights per sprout were heavier in BA treated sprouts than in no treated ones but the weights of the former sprouts were not influenced by its different concentrations.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.606-611
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• 2006

Decay during soybean sprout culture detracts their quality as well as increases their production costs. This study was done to determine the effects of acetic and propionic acids on growth and morphological characters of the sprouts. The soybean seeds of 3 cultivars (cv. Eunhakong, Pungsannamulkong and Orialtae) imbibed for 2 minutes at their different concentrations (0, 0.1 and 0.2%) were soaked for 6 hours in 4 ppm BA solution after the first 5.5 hour water imbibition and 0.5 hour aeration, and cultured at $20^{\circ}C$. On the 6th day, harvested soybean sprouts were classified into 4 categories on the base of hypocotyl length;>7cm, 4 to 7cm,<4cm and not germinated, and their morphological characters, fresh weights, lesion spots on cotyledons were measured or analyzed. The stronger concentration the higher rate of longer than 4cm although there was no significant difference between the two acids. Pungsannamulkong showed the longest hypocotyls but Eunhakong did the thickest ones, and Orialtae did the greatest total fresh weight, in which all the characters were not influenced by the two acids and their concentrations. Lesion spots on the cotyledons were equal to the two acids although less in their treatments than in no treatment. Utilization of propionic acid was more desirable Dan acetic acid through their treatment cost analysis.

• Journal of Bio-Environment Control
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• v.11 no.1
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• pp.23-28
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• 2002

This experiment was conducted far the efficient use of plant growth retardants (PGR) for suppression of plug seedling height. 'Seokwang' tomato seeds were soaked in I5 mL solution of daminozide (1,000, 10,000, or 100,000 mg.L-1) or uniconazole (1, 10, or 100 mg.L-1). And then, they were put in $25^{\circ}C$ chamber for one or three days. Seeds were washed in tap waters and were dried in 5$^{\circ}C$ chamber for one day. Dried tomato seeds were sown and seedlings were raised in 288-cell plug trays. Seedlings grown were evaluated at 21 and 38 days after sowing. Treatments of 10 or 100 mg.L-1 uniconazole as compared to the control had significantly suppressed hypocotyl length and seedling height, but fresh and dry weights of roots, emergence, no. of leaves, chlorophyll concentration, dry matter, and T/R ratio were not significant by different among treatments.

• Journal of Life Science
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• v.9 no.1
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• pp.29-34
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• 1999

Cotyledon and hypocotyl explants of perilla were cultured on MS medium containing a combined concentration of BA(0.5, 1.0 and 1.5mg/$\ell$) and NAA(0.1, 0.5 and 2.0mg/$\ell$) in order to regenerate the explant and induce the callus. The best regeneration of the explant and induction of the callus were observed in a combined concenteration of 0.5mg/$\ell$ of BA and 0.5mg/$\ell$ NAA both in cotyledon and hypocotyl explants. In cotyledon explants, rooting was achieved upon transferring shoots to MS medium containing 0.5mg/$\ell$ of BA and 0.1mg/$\ell$ of NAA. We also investigated the change of protein and RNA content on developmental stage of callus and plant regeneration of perilla. Protein content was increased but RNA content was decreased as the culture period increases. The banding pattern of polypeptide revealed that both 30KD and 45KD polypeptides were obvious in cotyledon obtained from pre-culture explants, but only 30KD polypeptide was further getting obvious as the culture period increases.