• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.35 no.5
• /
• pp.393-402
• /
• 1990

Studies to obtain a fundamental data to improve the quantity and quality of wheat protein and to select the excellent breeding materials suitable for various uses of it were performed on the focuses of protein content, amino acid composition, and its related quality. The range of seed protein content of crossing block consisted with 319 varieties or lines was from 9.9% to 17.6% with the average of 13.2%. The minimum protein content was showed by Wonkwang, and the maximum by Chungnamjaerae and Suwon 210. Among the Korean native varieties or breeding lines of crossing block, at least twelve lines or varieties were selected as the high-protein wheats. In the effects of environmental conditions, the variations of protein content by cultivating locations and year were showed out to be up to 3.6%, and 3.3%, respectively. But the varietal characteristics of wheat protein were seemed to remain constantly. In the results of amino acid analysis, those related with bread quality, except proline were improved with the increase of seed protein. But the improvement of nutritional quality did not agree with the protein content increase of seed. The total content of amino acid essential to adults was very low with 266.6-273.3mg per one gram of protein compare to the 350mg (free from the amino acid of tryptophan) recommended by FAO/WHO. Especially the limiting amino acid such as methionine, lysine and threonine were critically deficient. The correlation between protein contents and sedimentation values was obtained r=0.656$\^$**/ of average notwithstanding widly different environmental conditions, and in some condition the value was obained about to r=0.956$\^$**/.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.6 no.1
• /
• pp.61-65
• /
• 1977

Amino acids of duck-meat in Jeon-nam have been analyzed by gas chromatography. Through this analysis, the following results were obtained. 1. The quantities of general components in duck-meat were determined $13.61%{\sim}21.19%$ protein, $17.32%{\sim}34.92%$ fat, $0.56%{\sim}1.09%$ ash, and $50.91%{\sim}65.72%$ moisture. 2. The quantities of protein amino acids in duck-meat were determined. 6.10% alanine. 2.75% valine, 7.13% glycine, 2.20% iso-leucine, 4.54% leucine, 4.90% proline, 5.80% threonine, 1.15% methionine, 3.20% hydroxy proline, 3.01% phenylalanine, 6.70% asparticacid, 12.71% glutamic acid, 4.95% lysine, 1.11% arginine, 5.60% histidine, 4.40% cystine+cystein, and serine and tyrosine in trace amount. 3. Except tryptophane, almost all the essential amino acids were analyzed.

• Korean Journal of Microbiology
• /
• v.33 no.2
• /
• pp.86-91
• /
• 1997

Pseudomonas sp. DJ77로부터 클로닝된 glutathione S-transferase 유전자(phnC)의 염기서열을 결정하였다. 603bp의 open reading frame(ORF)이 존재하였고 개시코돈 앞에서 Shine-Dalgarno sequence를, 종결코돈 뒤에서는 terminator sequence를 발견하였다. phnC 유전자에서 만들어지는 phnC 단백질은 21,416 Da으로 SDS-polyacrylamide gel 전기영동 결과와 일치하였다. PhnC는 Bulkholderia cepacia LB400, Cycloclasticus oligotrophus RB1의 GST와 각각 53.7%, 49%의 높은 상동성을 나타냈다. 아미노산 서열의 상동성과 필수잔기들의 존재유무로 판단할 때 PhnC GST는 theta class GSTs와 진화적으로 유연관계가 높았지만 alpha, mu, pi, sigma class GSTs에서 구조적, 기능적으로 중요하다고 알려진 아미노산 잔기들이 PhnC GST에도 보존되어 있었다. 또한, phnC 유전자의 위치가 C. oligotrophus RB1, B. cepacia LB400 등의 GST 유전자 위치와 유사하다는 점에서 PhnC 효소는 난분해성 방향족 탄화수소의 분해에 관여하는 것으로 생각된다.

• Proceedings of the Korean Information Science Society Conference
• /
• 2003.10b
• /
• pp.832-834
• /
• 2003

단백질은 생명현상 유지에 필수적인 기능을 담당하며 이러한 기능이 단백질의 3차 구조에 의해 결정된다는 것이 밝혀짐으로써 단백질 3차 구조에 대한 연구가 더욱 활발히 진행되고 있다. 본 논문에서는 단백질의 3차 구조를 파악할 수 있는 Java3D 기반의 단백질 구조 뷰어인 KPDBViewer에 대하여 기술한다. 개발된 KPDBViewer는 3차원 이미지 상에서 단백질 내 아미노산들의 이벤트 처리를 지원함으로써 단백질내 아미노산의 정보를 보다 효과적으로 파악할 수 있다 또한, Java2D 기반의 단백질 뷰어는 다양한 구조 보기 기능이 부족하다. 이와 같은 기능을 제공함으로써 단백질 구조 정보를 보다 쉽게 이해하는데 도움을 줄 것으로 기대한다.

• Proceedings of the Korean Journal of Food and Nutrition Conference
• /
• 2000.12a
• /
• pp.53-53
• /
• 2000

최근 복분자(산딸기)의 효능을 이용한 일반식품 및 기능성 식품 등의 제품 개발이 활발히 추진되고 있다. 따라서 본 연구에서는 식품차원의 연구분야 일환으로서 먼저 복분자의 일반성분, 유리당, 유리아미노산, 무기성분 및 향기성분 등을 보고한 후 앞으로 생리 활성 연구 분야에 초점을 두고 연구를 진행할 하여 학문적 자료 및 산업에 응용할시 기초자료로 활용하고자 한다. 복분자의 수분은 5.39%, 총당은 17.3%, 환원당은 8.6%, 조단백질 10.6%, 조회분은 4.5%, 조지방은1.7%, 조섬유는 3.9%이고, 유리당 함량은 sucrose 1.52%, fructose 3.98%, glucose 1.24%이고, 유기산 함량은 citric acid 10.2%, oxalic acid 6.29%로 가장 많이 함유하고 있었고, 그 다음은 malic acid 1.94%였다. 총 아미노산은 7914.1mg%이고, 필수아미노산 함량은 2622.5mg%로 나타났다. 복분자에 가장 많이 함유되어 있는 아미노산은 aspartic acid 1260.3%, 그 다음은 glutamic acid 1054.3% 순으로 함유하고 있었다. 무기성분 K가 387mg으로 가장 많이 함유되어 있었고, 복분자에서 총 52종의 성분이 화인되었는데 확인된 성분들은 관능기별로 분류하면 amylalcohol를 포함한 alcohol류 11종, valeric acid를 포함한 acid류 13종, hexanal를 포함한 carbonyl류 20종, 2-heptanone를 포함한 hydrocabon류 5종 그리고 methyl palmitate를 포함한 ester류가 3종 이었다. 관능기별로 확인된 성분들의 총 peak area%를 살펴보면 alcohol류 11.56%, acid류 38.87%, hydrocarbon류 2.89%, ester류 0.80%, 총 70.32%가 확인되었다. Alcohol류에서 linalool가 3.78%, acid류에서는 caproic acid류가 14.40%, carbonyl류에서 2-hydroxy-4-methoxyacetophenone이 2.99%, hydrocabon류에서는 aromadendrene가1.59% 그리고 ester류에서는 methyl palmitate가 0.43%으로 가장 많이 함유하고 있었다.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.45 no.7
• /
• pp.980-989
• /
• 2016

In the present study, we compared and investigated the nutritional compositions of Amaranthus cruentus and Amaranthus hypochondriacus baby-leaves cultivated in Korea. Baby-leaves of two amaranthes consisted of more than 92% moisture, and A. cruentus contained a higher amount of moisture than A. hypochondriacus. Meanwhile, A. hypochondriacus contained higher levels of crude ash, crude protein, crude lipid, carbohydrates, and dietary fiber than A. cruentus. The major free sugars of the two amaranth baby-leaves were fructose and glucose. Fructose content of A. hypochondriacus was higher than that of A. cruentus, and glucose content of A. cruentus was higher than that of A. hypochondriacus. Acetic acid, malic acid, and fumaric acid were detected in two amaranth leaves, but succinic acid was not detected. Two amaranth leaves contained 17 amino acids except for methionine, proline, and tyrosine, and leaves contained the highest glutamic acid contents. In addition, A. cruentus and A. hypochondriacus leaves contained high contents of taurine and ${\gamma}$-aminobutyric acid and showed various biological activities. The major mineral and fatty acid of the two amaranth leaves were potassium and linolenic acid (C18:3), respectively. The ${\beta}$-carotene contents of A. cruentus and A. hypochondriacus leaves were $478.72{\mu}g/100g$ and $474.12{\mu}g/100g$, respectively. In vitamin B complex, $B_2$, $B_3$, and $B_5$ were detected in the two amaranth leaves whereas vitamins $B_1$, $B_6$, and $B_{12}$ were not detected. A. hypochondriacus contained higher amounts of vitamin C and E than those of A. cruentus. Overall, amaranth leaves contained high amounts of nutritional components. Therefore, amaranth leaves are expected to be useful for the development of a functional food. Moreover, these results will provide fundamental data for advancing sitological value, breeding new cultivars, and promoting leafy vegetable usage.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2004.10a
• /
• pp.257-262
• /
• 2004

제주 재래마육의 영양학적 가치 구명을 위하여 재래마육 등심과 볼기부위를 분석한 결과 일반성분에서 수분 및 단백질함량에서 부위별 차이가 있었으며 콜레스테롤 함량은 등심과 볼기부위가 각각43.5 mg% 그리고 43.2 mg%였다. 마육에서 미네랄함량은 K가 가장 많았으며 다음으로 P>Na>Mg>Ca순이 었다. Vitamin A 함량은 등심과 볼기부위가 각각 9.62IU, 8.54 lU 였다. 등심과 볼기부위의 필수지방산이 각각 11.0%, 13.0%, 불포화지방산은 각각 62.6%, 63.7% 로 높게 나타났다. 함황아미노산은 경우 등심과 볼기부위가 각각 927.4 mg%, 1041.9 mg%였으며, 필수아미노산의 함량 비율은 각각 49.2 %, 49.7%로 나타났다.

• Applied Biological Chemistry
• /
• v.35 no.1
• /
• pp.64-67
• /
• 1992

To extract insoluble proteins of soymilk residue by microorganism, the soymilk residue was treated with crude enzyme solution from Aspergillus oryzae. Optimum conditions of pH, temperature and digestion time were determined, and amino acid composition of the extract was analyzed. The optimum pH for the extraction was 7.5, and the maximum extraction was obtained at $50^{\circ}C$. Under optimum conditions, the extractability with Koji reached to 70% in 12 hrs. The content of essential amino acids of extract was generally high and the composition of essential amino acid was good.

• The Korean Journal of Mycology
• /
• v.24 no.3 s.78
• /
• pp.176-179
• /
• 1996

Media development of Pleurotus ostreatus were analyzed to recognize the compositional differences depending on rice straw and waste cotton stuff media. Yields of add the rice bran 20% to the waste cotton stuff were increment 11% as compared with rice straw media. Crude protein of products to the waste cotton stuff media on Pleurotus ostreatus was a much and crude ash was less than of rice straw media. Glutamic acid was most and cystine was lowest among the amino acids of Pleurotus ostreatus. Total amino acids and essential amino acids of waste cotton stuff were much than of rice straw media.

• Korean Journal of Food Science and Technology
• /
• v.43 no.6
• /
• pp.729-734
• /
• 2011

With the goal of transforming rice protein from an insoluble to a soluble form to increase the industrial utilization of rice wine meal (RWM), RWM was derivatized using commercial proteases and the RWM hydrolysates were characterized. Eight commercial proteases were used individually or in combination for hydrolysis of RWM. The degree of hydrolysis was assessed by determining the soluble protein in supernatant using the Lowry assay, protein in precipitates using a semimicro Kjeldahl procedure, and gravimetrically by the weight difference before and after hydrolysis. Protamex, Alcalase and Protease N proteases were most effective for hydrolysis of RWM. Although these assessment methodologies displayed some variation, they generally showed a similar pattern. When the aforementioned three proteases were simultaneously used to treat RWM, no significant difference was observed between the three assays (p<0.05) indicating an absence of enzymatic synergy.