• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.88-93
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• 2007

The purpose of the study reported here was to test the hypotheses that clinically healthy dogs will not manifest immediate hypersensitivity responses to intradermal injection of Malassezia pachydermatis extracts but that affected dogs with Malassesia otitis will manifest such hypersensitivity. Wd desired to identify approximate molecular mass of any allergenic components of the yeast by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein profile of Malassezia pachydermatis extracts showed between 16 and 110 kDa. Especially, the intensity was strongest between 25 and 80 kDa. Mean wheal diameters in the affected groups of 20, 2, 0.2, and $0.02{\mu}g/ml$ were $13.36{\pm}0.67,\;5.33{\pm}0.67,\;5.47{\pm}0.82,\;and\;5.07{\pm}0.64$, respectively. Mean wheal thickness in the affected groups of 20, 2, 0.2, and $0.02{\mu}g/ml$ was $6.44{\pm}0.40,\;3.86{\pm}0.35,\;2.64{\pm}0.36,\;and\;2.60{\pm}0.44$, respectively. The difference of wheal diameters and thickness between healthy and affected groups was significant (p<0.05). In conclusion, the observations confirm that Malassezia pachydermatis-derived antigens may induce an immediate wheal response when intradermal injected in dogs. It seems reasonable to suggest that hypersensitivity to yeast may contribute to the development of clinical signs in dogs with immediate skin test reactivity, especially in dogs with Malassezia otitis extema.

• Journal of Sericultural and Entomological Science
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• v.45 no.2
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• pp.108-111
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• 2003

A infectious fungus (J131) recently isolated from artificial cultivation farms of Paecilomyces tenuipes is known to cause a great yield loss and deterioration of products. In this study, its occurrence and characterization were made. The isolate (J131) was identified as Paecilomyces farinosus (Holm ex S. F. Gray) Brown ＆ Smith. Colonies of the isolate on PDA grew moderately fast, attaining a diameter of 19mm within 14 days at 24${\pm}$1$^{\circ}C$. Vegetative hyphae are smooth-walled and hyaline. Conidiophores mainly arose from the submerged mycelium, usually measuring 1.3-1.5$\mu\textrm{m}$ wide and consisted of verticillate branches with whorls of 2 to 4 phialides. Phialides of 3.8-5.8${\times}$1.5-1.8 $\mu\textrm{m}$, with a swollen basal portion tapered into a distinct neck of about 0.6-0.8 $\mu\textrm{m}$ wide. Conidia arisen from the phialides, were ellipsoidal to fusiform, smooth-walled, hyaline, 1.8-2.4${\times}$1.1-1.4$\mu\textrm{m}$. .When silkworm larvae were injected with concentration of 1${\times}$10$\^$8/ conidia/$m\ell$ of infectious fungus J131, infection rate was 41.3％ and synnemata formation rate was 23.6％. Black spots were appeared on the surface of silkworm larvae in 5-9 days after injection. The surface of larvae was covered with hyphae which were gradually turned into yellow from white color during the incubation period of 3-4 days. 1 to several 4-6mm long synnemata of yellow or orange color were formed on the surface. The matured synnemata were ramified from its end and produced much conidia of white color.

• Journal of Life Science
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• v.33 no.10
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• pp.842-850
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• 2023

Pyrrolnitrin, pyrrolomycin, and pyoluteorin are functional halogenated phenylpyrrole derivatives (HPDs) derived from microorganisms with diverse antimicrobial activities. Pyrrolnitrin is a secondary metabolite produced from L-tryptophan through four-step reactions in Pseudomonas fluorescens, Burkholderia cepacia, Serratia plymuthica, etc. It is currently used for the treatment of superficial dermatophytic fungal infections, has high antagonistic activities against soil-borne and foliar fungal infections, and has many industrial applications. Since pyrrolnitrin is easily decomposed by light, it is difficult to widely use it outdoors. As an alternative, fludioxonil, a synthetically produced non-systemic surface fungicide that is structurally similar and has excellent light stability, has been commercialized for seed and foliar treatment of plants. However, due to its high toxicity to aquatic organisms and adverse effects in human cell lines, many countries have established maximum residue levels and strictly control its levels. Pyrrolomycin and pyoluteorin, which have antibiotic/antibiofilm activity against Gram-positive bacteria and high anti-oomycete activity against the plant pathogen Pythium ultimum, respectively, were isolated and identified from microorganisms. This review summarizes the biosynthesis and production of natural pyrrolnitrin derived from bacteria and the characteristics of synthetic fludioxonil and other natural phenylpyrrole derivatives among the HPDs. We expect that a plethora of highly effective, novel HPDs that are safe for humans and environments will be developed through the generation of an HPD library by microbial biosynthesis and chemical synthesis.

• Pediatric Infection and Vaccine
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• v.12 no.2
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• pp.114-123
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• 2005

Purpose : A wide, epidemic outbreak of M. pneumoniae pneumonia occurred throughout Korea in late 2003. Compared with previous years, the 2003 outbreak resulted in more severe cases and in an increased incidence of extrapulmonary symptoms and/or complications. We compared the clinical characteristics for M. pneumoniae pneumonia of 2003 to those of the past years. Methods : One hundred six children diagnosed with M. pneumoniae pneumonia by serologic tests at Bundang Cha General Hospital between Aug 2003 to April 2004 were enrolled. Medical records were reviewed retrospectively, for clinical, laboratory and radiological aspect as well as complications. The pleural effusions of 3 patients who underwent thoracentesis were also analyzed. Results : The duration of fever, cough, rhinorrhea, and sore throat was $8.2{\pm}4.7$, $22.1{\pm}4.8$, $8.4{\pm}2.1$, $4.3{\pm}1.2$ days, respectively. The incidence (percentage) and duration of abdominal pain, vomiting, diarrhea, headache, skin rash, arthralgia was $5.1{\pm}2.5$ (21.9%), $3.4{\pm}2.1$ (17.1%), $4.3{\pm}1.8$ (16.2%), $3.5{\pm}2.1$ (14.4%), $5.5{\pm}0.7$ (5.9%) and $4.6{\pm}1.3$ days (4.9%), respectively. The mean duration of admission and treatment were $7.4{\pm}4.3$ days and $21.6{\pm}11.1$ days. Higher values of CRP and ESR on admission were positively correlated with the duration of fever and length of admission. The findings of pleural effusion were similar to those seen in TB pleurisy. Complications, including myocarditis (2 cases), arthritis (3 cases), vasculitis (5 cases), asthma (3 cases), ARDS (1 case), and DIC (2 cases) were observed in 14.1% of patients. Conclusion : We found a number of characteristics of M. pneumoniae pneumonia among cases from late 2003 that were different from those of previous years. This outbreak resulted in more severe cases and in an increased incidence of extrapulmonary symptoms and/or complications. A multicenter study is needed to verify the changes in clinical characteristics observed during the 2003 outbreak from previous ones.

• The Journal of Korea Assosiation for Disability and Oral Health
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• v.9 no.2
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• pp.111-117
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• 2013

Some odontogenic infections erode into fascial spaces directly and spread toward lymphatic tissues and blood streams. The principal maxillary primary spaces are the canine, buccal, and infratemporal space, the next secondary spaces are the masseteric, temporal and pharygeal space. As a result of the infection, trismus and orocutaneous fistula may be occurred. Trismus is owing to conditions not associated with temporomandibular joint itself and may be of myogenic, neurogenic, or psychogenic nature. Muscular trismus is due to infection adjacent to the elevator muscles of the jaw. The four principles of treatment of infection are as follows: (1) removal of the cause, (2) establishment of drainage, (3) institution of antibiotic therapy, and (4) provision of supportive care, including rest, nutrition and physiotherapy. Jaw physiotherapy is necessary to increase the amount of mouth opening and regain normal muscle tone. If proper care of odontogenic infection could be attained, the orocutaneous fistula will heal and close spontaneously by wound contraction mechanism of natural homeostatic response. This is a case report of the care of trismus and orocutaneous fistula due to fascial space abscess by advanced odontogenic infection in a physically disabled patient.

• Journal of the Korea Convergence Society
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• v.7 no.6
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• pp.75-80
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• 2016

Distraction osteogenesis is applied to correct mandible or maxilla deformities. Owing to the distractor being away from the skin, it is not aesthetic. Infection can arise due to manual part. And therefore, it is necessary to make the distractor using a new mechanism. 3D software was used for the intraoral distractor modeling. 3D meshes were used to analyze the stress distribution of the distractor was analyzed using Finite element analysis software. This research developed a intraoral distractor for continuous mandible distraction through convergence study. Base on the results, it provide helpful data for future version of the wireless intraoral distractor in other fields of bio and medical engineering.

• Journal of Veterinary Clinics
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• v.8 no.2
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• pp.147-152
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• 1991

Trichophyton mentagrophytes was described as a primary cause of mycotic dermatitis in two young goats housed together in a humid, ill-ventilated and unhygienic byre. The diagnosis in both the cases was established on the detection of fungal element in the skin scrapings by potassium hydroxide technique and isolation of the pathogen in pure growth on mycological medium at 30$^{\circ}C$. The lesions were found on the face of one kid and on the neck and ear of another one. Two adult goats housed in the same enclosure were found to be free from this disease. Further, there was no evidence of ringworm in the goat owner and his family members. Genetic crossing of both the isolates on modified sunflower seed medium indicated that they belonged to (―) mating type. Hair performation test revealed the keratolytic activity of both the strains of T. mentagrophytes. The public health significance and chemotherapy are also discussed. The question of source of infection could not be emphatically established.

• Journal of Veterinary Clinics
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• v.26 no.4
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• pp.391-394
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• 2009

An Asian water monitor Varanus salvator with physical wound due to bite which was subsequently infected with bacterium resulting to hemorrhage and pus in the skin blisters, abdominal distention and septicemia. Morganella morganii was isolated and identified from the blood and kidney of the reptile, and confirmed by PCR and biochemical tests. The sensitivity of isolated strains to different groups of antibiotics was also evaluated using the disc diffusion method. Pathogenicity test using M. morganii (SNUFPC-MM01) (1.6 ${\times}$ $10^{11}$CFU/mouse) to suckling and adult mice resulted to the death of all mice. This paper describes the first isolation of M. morganii from Asian water monitor in Korea.

• Korean Journal of Veterinary Research
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• v.13 no.2
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• pp.165-170
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• 1973

1972년 여름, 사관생도(士官生徒) 승마교육용(乘馬敎育用)으로 육군사관학교(陸軍士官學校) 군마대(軍馬隊)에서 사육(飼育)하고 있는 승마용(乘馬用) 마필(馬匹)(오스트레일리아 및 일본산(日本産))과, 군용마(軍用馬)로서의 개발시험(改發試驗)에 공시중(供試中)인 제주도산(濟州道産) 재내마필(在來馬匹)에 발생(發生)한 14예(例)의 피부사상균성(皮膚絲狀菌性) 윤선(輪癬)을 대상(對象)으로 그 치료시험(治療試驗)을 실시(實施)하였던 바, 다음과 같은 결론(結論)을 얻었다. 임상증례(臨床症例) 관찰(觀察) (1) 초기증상(初期症狀) 발현(發現)은 군마고유번호(軍馬固有番號) 15번(番), 마명(馬名) 거상(巨象)(원명(原名) Shane)의 이마 및 이근부(耳根部) 견부(肩部)에 직경(直徑) 3cm 정도(程度)의 원형(圓型) 병소(病巢)가 나타났으며, 곧 이어 다른 마필(病巢)에 산발적(散發的)으로 발생(發生)했다. 발생률(發生率)은 31.8%(군마(軍馬) 44%, 조랑말 15.8%)로서 지난해 군부대(軍部隊) 목장(牧場)에서 집단발생(集團發生)했던 Bovine Ringworm의 발생률(發生率)(56.4%)보다 낮았다. (2) 증상(症狀)은 원형발진(圓型發疹) 및 양감(痒感), 가피형성(痂皮形成), 피모탈낙(皮毛脫落), 농포형성(膿疱形成) 및 신모발생등(新毛發生等)의 순(順)으로 진행(進行)했으며 환축(患畜)과 접촉(接觸)하는 사람에게도 감염(感染)하는 인축공통전염병(人畜共通傳染病)의 일종(一種)이었다. (3) 품종(品種) 및 성별(性別), 연령에 따른 유의성(有意性)은 인정(認定)할 수 없었다. 치료시험(治療試驗) 결과(結果) (1) 본병(本病)은 Ichthammol이나 Undecylenic Acid 처리(處理)에 있어서 치료효과(治療效果)를 나타냈다. (2) 상기(上記)한 항사상균제 처리(處理)와 동시(同時)에 Hydrccortisone의 보조요법(補助療法)을 실시(實施)하였을때, 약효(藥效)가 빨리 나타나고 치료기일(治療期日)도 단축(短縮)되었다. (3) 본증(本症)은 조기(早期)에 발견(發見) 치료(治療)해야하며 일반적(一般的)으로 완치(完治)하는데 장기간(長期間)을 요(要)하였다.

• The Journal of Korean Physical Therapy
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• v.9 no.1
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• pp.177-184
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• 1997

1. The human body is the unification related to the powerful fascial network, I think. 2. Myofascial not only prevent and support the human body structure curdling but also keep the physical balance by dispersing traumatization properly. 3. Myofascial restriction will be developed into muscle deficiency and cause pains without releasing the muscle tension and the spasm. 4. Myofascial restriction affect and change the physical posture by losing the muscle elasticity and flexibility and by losing muscle supporting ability from gravitation. 5. The partial myofascial restriction affect the muscle and the adjoining joint supporting gravitation and cause the unbalance of the entire body.