• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.60-60
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• 2020

본 연구는 수확 단계에 따른 작약 주요 품종의 수확후 품질 특성을 조사하여 품종별 적정 수확 시기를 구명하고자 수행하였다. 절화 작약은 '두체스', '레드참', '보울오브크림' 품종을 사용하였으며, 세 품종 모두 1, 2, 3, 4단계로 구분 지어 수확하여 실험에 사용하였다. 수확한 작약은 40cm로 재절단하여 chrysal professional III 용액이 담긴 화병에 꽃아 생체중, 증산량, 수분흡수량, 개화 단계 변화 등을 매일 조사하였고, 이를 통해 수분균형 및 절화 수명 등의 결과를 도출하였다. '두체스' 품종의 품질 특성 변화를 조사한 결과, 1단계에 수확한 절화의 만개소요일수는 5.5일, 만개유지일수는 2.9일, 총 절화수명은 8.4일로 다른 수확단계보다 1~3일 더 긴 것으로 조사되었으며, 생체중증가율 및 화경 변화 결과를 통해 1단계 수확 시에도 개화는 정상적으로 진행되는 것을 확인할 수 있었다. '레드참' 품종의 품질 특성 변화 조사 결과, 1단계에 수확한 절화의 만개소요일수는 7.9일, 만개유지일수는 3.3일, 총 절화수명은 11.2일로 다른 수확단계보다 1~4일 더 긴 것으로 조사되었다. '보울오브크림' 품종의 품질 특성 변화 조사 결과, 1단계 수확한 절화는 30% 수준만 만개하여 조기 수확에 따른 문제점이 확인되었지만, 2단계 수확한 절화는 개화와 만개 모두 100% 진행되었으며, 만개소요일수는 6.4일, 만개유지일수는 4.5일, 총 절화수명은 10.9일로 다른 수확단계보다 1~2일 긴 것으로 조사되었다. 본 연구 결과, '두체스' 와 '레드참' 품종의 적정 수확단계는 1단계, '보울오브크림' 품종의 적정 수확단계는 2단계가 적절할 것으로 판단된다.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.366-373
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• 2011

To analyze the genetic relationships among Korean potato cultivars and to develop cultivar identification method using DNA markers, we carried out genotyping using simple sequence repeats (SSR) analysis and developed multiplex-SSR set. Initially, we designed 92 SSR primer combinations reported previously and applied them to twenty four Korean potato cultivars. Among the 92 SSR markers, we selected 14 SSR markers based on polymorphism information contents (PIC) values. PIC values of the selected 14 markers ranged from 0.48 to 0.89 with an average of 0.76. PIC value of PSSR-29 was the lowest with 0.48 and PSSR-191 was the highest with 0.89. UPGMA clustering analysis based on genetic distances using 14 SSR markers classified 21 potato cultivars into 2 clusters. Cluster I and II included 16 and 5 cultivars, respectively. And 3 cultivars were not classified into major cluster group I and II. These 14 SSR markers generated a total of 121 alleles and the average number of alleles per SSR marker was 10.8 with a range from 3 to 34. Among the selected markers, we combined three SSR markers, PSSR-17, PSSR-24 and PSSR-24, as a multiplex-SSR set. This multiplex-SSR set used in the study can distinguish all the cultivars with one time PCR and PAGE (Polyacrylamide gel electrophoresis) analysis and PIC value of multiplex-SSR set was 0.95.

• Horticultural Science & Technology
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• v.32 no.4
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• pp.525-534
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• 2014

Microsatellite markers were used to identify 58 major commercial melon cultivars, and to assess hybrid seed purity of a melon breeding line known as '10H08'. A set of 412 microsatellite primer pairs were utilized for fingerprinting of the melon cultivars. Twenty-nine markers showed hyper-variability and could discriminate all cultivars on the basis of marker genotypes, representing the genetic variation within varietal groups. Cluster analysis based on Jaccard's distance coefficients using the UPGMA algorithm categorized 2 major groups, which were in accordance to morphological traits. The DNA bulks of female and male parents of breeding line '10H08' were tested with 29 primer pairs based on microsatellites to investigate purity testing of $F_1$ hybrid seeds, and 5 primer pairs exhibited polymorphism. One microsatellite primer pair (CMGAN12) produced unambiguous polymorphic bands among the parents. Among 192 seeds tested with CMGAN12, progeny possibly generated by self-pollination of the female parent were clearly distinguished from the hybrid progeny. These markers will be useful for fingerprinting melon cultivars and can help private seed companies to improve melon seed purity.

• Journal of Mushroom
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• v.17 no.4
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• pp.171-178
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• 2019

Agaricus bisporus is a functional food and among the most widely cultivated mushrooms in the world. In this study, we analyzed the genetic diversity and population structure of 23 Korean and 42 foreign A. bisporus cultivars using SSR (Simple sequence repeat) markers. Genetic diversity of A. bisporus cultivars was as follows: number of alleles was approximately 13; observed and expected heterozygosity were approximately 0.59 and 0.74, respectively; and polymorphic information content value was about 0.71. A. bisporus cultivars were divided into three groups using distance-based analysis. Genetic diversity of Group 2, which consisted of cultivars from various countries, was high. In addition, model-based subpopulations were divided into two, and the genetic diversity of Pop2 (Population 2), which had many cultivars, was high. The results of this study could be used in a breeding program for A. bisporus, such as the development of new genetic resources and securing diversity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.3
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• pp.277-285
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• 1986

A total of 16 varieties from Korea and Indonesia were tested at Suwon, Korea (126$^{\circ}$19'E, 37$^{\circ}$16'N and 37m above sea level) and Bali, Indonesia (115$^{\circ}$ 14'E, 8$^{\circ}$42'S and 10m above sea level). Japonica-type varieties showed pre-matured heading at Bali, tropical zone, while Bulu varieties showed no heading at Suwon, temperate zone. The varieties of Indica-type and Ind./Jap. showed faster heading in tropical zone than in temperate zone. Dry matter of the varieties weighed more difference among varietal groups in tropical than in temperate area, while plant height responsed differently depending on variety and test region.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.2
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• pp.86-90
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• 2014

This study was conducted to examine the state of confusion about those azalea cultivars(hereinafter, Azalea Cultivars) that are used among practicians in landscaping businesses(designers, constructors, and producers). Which Azalea Cultivars would be delivered when they were ordered to producers using the names of Azalea Cultivars notified by the Public Procurement Service. In addition, the situations of azalea cultivar planting designs in the planting design drawings of three apartments completed recently and whether the Azalea Cultivars on the design drawings were the same as those Azalea Cultivars that were actually planted were examined. When Azalea Cultivars were ordered using the notified names, three Azalea Cultivars; Beni, Three, and Daewang were delivered for the name Yeongsanhong and two Azalea Cultivars; Zasanhong and Hancheol were delivered for the name Zasanhong. Yeongsanhong was perceived as a common name of Beni, Three, and Daewang by producers. Therefore, this caused confusion between producers and purchasers. In the case of Zasanhong, as the traits of Zasanhong are similar to those of the cultivar Hancheol, the producers or purchasers might have been unable to clearly distinguish between the cultivars and thus two cultivars might have been delivered without being distinguished from each other. The names of Azalea Cultivars used in planting design drawings were the names notified by the notifying agencies or comprehensive and unclear names such as azalea and Yeongsanhong. Completely different Azalea Cultivars from those written in planting design drawings were planted in actual construction sites in many cases. These situations should have occurred because designers did not write accurate cultivar names in planting design drawings and constructors could not accurately perceive the classification characteristics of individual Azalea Cultivars but planted those Azalea Cultivars with similar flowers colors or those that could be easily bought to fit the conditions of construction sites.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.711-715
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• 1995

This study was carried out to determine the desirable screeing condition for low-temperature germinability of rice seeds. The germination test was performed with fifty rice varieties from three countries, mainly including the recent Korean rice cultivars. The analyzed result of germination test under three temperature conditions(13$^{\circ}C$, 16$^{\circ}C$, $25^{\circ}C$) pointed out that 11 days after seeding at 13$^{\circ}C$ was the most efficient testing condition for low-temperature germinability in terms of mean and variation in germination rate. Highly significant correlations were observed between germination rates of 11 days after seeding at 13$^{\circ}C$ and germination coefficients, average days of germination at 13 for 20 days. Low-temperature germinability were highly positively associated with days to heading or amylose content, but negatively correlated with grain width.

• Korean Journal of Breeding Science
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• v.41 no.2
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• pp.101-105
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• 2009

Rice (Oryza sativa L.), the world's most important crop, is usually classified into ssp. indica and japonica based on morpho-physiological traits. In the previous study, we have developed subspecies-specific STS markers (SS markers) to readily discriminate between indica and japonica in O. sativa. In this study, we employed SS markers to investigate the genomic inclination of worldwide collections of O. sativa. A total of 320 varieties were divided into two groups with 63 SS markers. Namely, they formed two distinctive groups, indica and japonica, as expected by their geographic origin. The population structure analysis revealed that the variability of genetic background was greater in indica than in japonica. Some of them, however, exhibited intermediate genomic inclination between indica and japonica. These results are in general agreement with the previous studies, suggesting that SS markers are powerful tools for both determination of subspecies genome and assessment of genetic diversity in rice.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.375-381
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• 2018

The consumption of oats (Avena sativa L.) with high nutritional utility is accelerating due to the increased consumers' demand for functional foods. In Korea, naked oats are used as food, while covered oats are used for animal feed. However, it is difficult to distinguish naked oats from covered oats when the husk is removed from the grains by a special process. The present study was carried out to investigate experimental methods that would be beneficial in the segregation of different types of oats after husk removal. Grain quality-related biochemical compounds were analyzed in a bid to differentiate the oat dehulling characteristics. In addition, 61 SSR markers were examined for genetic relationship and variety identification of oats using five naked and seven covered oat varieties. Results showed that, the contents of protein, lipid, and ${\beta}-glucan$ were not significantly different among the oat varieties and this could not be used as an index for distinguishing oats husk character. However, in the fatty acid composition ratio,, naked oats had a higher ratio of stearic acid (C18:0) and oleic acid (C18:1) than covered oats, and covered oats had a higher ratio of linoleic acid (C18:2) and linoleic acid (C18:3) than naked oats. The assessment of SSR marker genotype revealed that 33 polymorphic bands among 12 oat varieties and 1 variety could be distinguished through the combination of polymorphic markers thus indicating the usability of these markers for variety identification in oats.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.4
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• pp.236-245
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• 2023

We investigated the basic vegetative phase (BVP), photoperiod-sensitivity (PS), and thermo-sensitivity (TS) of 40 rice varieties to characterize their heading ecology that is adaptable to North Korean rice production areas. The ranges were 12 - 43 days for BVP, 0 - 74 days for PS, and 15 - 33 days for TS, depending on the variety. The number of days from transplanting to the heading stage (DTH) was significantly correlated with PS in the 13 major rice production regions where all 40 varieties (including early-, middle-, and mid-late-maturing varieties) were tested. DTH was significantly correlated with BVP and TS but not with PS in the 10 low-temperature regions where only 28 early-maturing varieties were tested. The heading ecology of the adaptable varieties for each region could be characterized by an exponential equation between the BVP and PS of varieties that headed at the border of the safe marginal heading date (SMHD) for each of the seven regional environments (Kaesong, Haeju, Yongyon, Singye, Sariwon, Nampo, and Pyongyang). A PS of 25 - 30 days or less was an additional adaptive trait in the Sariwon and Pyongyang environments. The varieties that reached the heading stage not later than the SMHD in six regional environments (Anju, Kusong, Sinuiju, Changjon, Wonsan, and Supung) and those that reached the heading stage not later than the late marginal heading date (LMHD) in four regional environments (Hamhung, Pyonggang, Huichon, and Kanggye) had both a PS of 26 days or less and a BVP of 25 - 28 days or less. In the Yangdok, Sinpo, and Chunggang environments, varieties that reached the heading stage not later than the LMHD for each region had a BVP of 15 - 20 days or less. The results suggested that a shortened BVP trait should be introduced to existing early-maturing rice varieties to reduce the duration of growth period to reach the heading stage.