• Journal of Plant Biotechnology
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• v.33 no.4
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• pp.283-288
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• 2006

Discrimination of 5 rice cultivars (Sangjubyeo , Dongjinbyeo Simbaekbyeo , Hwamanbyeo , and Simbaek-hetero ) using metabolic profiling was carried out. Whole cell extracts from each cultivar were subjected to $^1H$ NMR spectroscopy. When spectral data were analyzed by principal component analysis, 5 cultivars were clustered into 3 groups: SJ, DJ + SB, and HM + SH. Thecultivars showed great difference in carbohydrate region of $^1H$ NMR spectra, suggesting that qualitative and quantitative differences in carbohydrate compounds play a major role in discrimination of the cultivars. In addition, it was readily possible to determine relative quantification of major carbohydrates including sucrose, glucose, maltose from spectral data of the cultivars. SJ showed 2 to 4 times higher content of maltose than the other rice cultivars. Overall results indicate that metabolic discrimination of rice cultivars using $^1H$ NMR spectroscopy combined by multivariate statistical analysis can be used for rapid discrimination of numerous rice cultivars and simple quantitative analysis system of major carbohydrate compounds in rice grains.

• Applied Biological Chemistry
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• v.36 no.3
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• pp.139-145
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• 1993

The methods of isoelectric focusing of 4 isozymes in polyacrylamide horizontal slab gels and restriction fragment length polymorphisms (RFLPs) were applied to characterize the biochemical phenotypes of 19 cultivars of barley. Among 19 barley cultivars screened, 7 esterase, 3 phosphoglucose isomerase, 4 peroxidase and 2 alcohol dehydrogenase isozyme phenotypes were distinguished by isoelectric focusing. When purified DNA of each cultivar was digested with restriction enzyme EcoRV and analyzed its RFLPs with barley DNA markers pMSU 51 or pMSU 71, two distinct RFLP patterns were shown. Based on the four isozymes and two RELP polymorphisms, 19 cultivars of barley were classified into 13 biochemical phenotypes. Phylogenetical relationships among 13 biochemical phenotypes classified were determined using Nei's F-statistics and the phylogenetic dendrogram was constructed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.405-411
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• 1985

The buffer soluble proteins were extracted from six cultivars of barley grains and analyzed by various electrophoresis; 7.5% polyacrylamide slab gel, 2-30% polyacrylamide porosity gradient tube gel, isoelectric focusing (pH4-9) and starch gel electrophoresis. The proteins, esterase, acid phosphatase, malate dehydrogenase, glutamate dehydrogenase and leucine aminopeptidase were investigated to find out the best method to differentiate barley cultivars. The result were that protein and esterase bands in 2-30% polyacrylamide porosity gradient tube gel electrophoresis and protein bands in 7.5% polyacrylamide slab gel electrophoresis showed typical varietal differences. Therefore, those methods were suitable for differentiation of barley cultivars. It was difficult to differentiate the cultivars by the other methodes and patterns of the other enzymes.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.305-313
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• 2016

Brassica juncea (2n = 4x = 36, AABB genome, 1,068 Mb) is a U's triangle species and an amphidiploid derivative of B. rapa and B. nigra. Fifteen varieties were used to study the ITS (internal transcribed spacer) regions of ribosomal DNA and MITEs (miniature inverted-repeat transposable elements) with a view of developing specific molecular markers. ITSs and MITEs are an excellent resource for developing DNA markers for genomics and evolutionary studies because most of them are stably inherited and present in high copy numbers. The ITS (ITS1 and ITS2) sequence was compared with the consensus sequence of B. rapa and B. nigra. Variation in ITS1 created two separate groups among 15 varieties, with 10 varieties in one group and 5 in the other. Phylogenetic analysis revealed two major clusters for those 10 and 5 varieties. Among the 160 different MITE primers used to evaluate the selected 15 varieties of B. juncea, 70 were related to the Stowaway, 79 to the Tourist, 6 to the hAT, and 5 to the Mutator super-families of MITEs. Of 160 markers examined, 32 were found to be polymorphic when fifteen different varieties of B. juncea were evaluated. The variety 'Blackgat' was different from the other mustard varieties with respect to both phenotype and genotype. The diversity of 47 additional accessions could be verified using eight selected molecular markers derived from MITE family sequences. The polymorphic markers identified in this study can be used for varietal classification, variety protection, and other breeding purposes.

• Journal of Mushroom
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• v.19 no.4
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• pp.336-340
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• 2021

The cleaved amplified polymorphic sequence (CAPS) marker uses a restriction enzyme recognition site resulting from single nucleotide polymorphisms and insertions and deletions on the DNA sequence. This technique does not require expensive equipment, the process is simple, and clear results can be obtained reliably. In this study, Agaricus bisporus cultivars SaeA, SaeDo, SaeHan, SaeYeon, SaeJeong, Dodam, Seolgang, Dahyang, Hogam, and Hadam developed in Korea were discriminated using four CAPS markers. Our results indicated that it is possible to distinguish the ten cultivars and determine the genetic diversity among them.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.22-30
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• 2011

Weonhyeong is one of important commercial strains. It has good characteristics of bundle formation, grey colored pilei and high productivity. We previously reported grouping of 70 strains of Pleurotus ostreatus in which one group contained 35 strains including Weonhyeong. Four strains in that group showed same profiles implicating no variety distinction for mushroom cultivation. Now we developed a specific marker for identification of Weonhyeong. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. SCAR marker 'S-OPO5' produced only one band specific to 2183, 2240, 2595 and 2725 strains showing similar banding patterns to Weonhyeong in RAPD-PCR results. The sequence of 'S-OPO5' marker was unknown when compared with the data in the Genbank using BLASTN. BLASTX results indicated that the marker showed significant alignment with the protein sequences in Tricholoma bakamatsutake reverse transcriptase. The results indicate that this new SCAR marker ('S-OPO5') will be valuable to distinguish the Weonhyeong similar strains from Pleurotus spp.

• Journal of Life Science
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• v.16 no.2 s.75
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• pp.219-224
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• 2006

Inter-simple sequence repeat (ISSR) analysis were used to assess genetic diversity among 18 genotypes of watermelon (Citrullus lanatus Thunb.) including breeding lines and commercial varieties. The 21 ISSR primers selected from 100 primers were showed the amplification of 105 reproducible fragments ranging from about 200 bp to 5000 bp. A total of 58 DNA fragments were polymorphic with an average 2.7 polymorphic bands per primer. The polymorphic primers were divided into 18 anchored primers and 3 non anchored primers. All of the anchored primers were di-nucleotide repeat motif, and was more polymorphic than non anchored primers. Eighteen watermelon genotypes were classified into two large groups. Clustering was in some accordance with the division of fruit shape into 18 watermelon. Therefore, ISSR markers may be suitable for variety discrimination and for constructing a linkage map of watermelon.

• The Korean Journal of Mycology
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• v.25 no.3 s.82
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• pp.219-225
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• 1997

Random Amplified Polymorphic DNA (RAPD) assay was used to identify seven typical Lentinula edodes (Berk.) Pegler strains isolated in Korea. Twenty primers from OPA-01 to OPA-20 were applied to generate the recognition of L. edodes strains. Out of 20 primers, nine primers showed efficient RAPD patterns to classify the 7 strains tested, but the rest eleven primers were not useful to be used. Even though there was no single primer that could classify all of the strains, any combination of two primers among the nine primers could identify the strains tested. Thus, RAPD assay turned out to be very precise method for classifying L. edodes strains.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.84-84
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• 2018

참억새(Miscanthus sienesis)는 화본목 벼과 억새속에 속한 다년생 단자엽식물로 동아시아와 오세아니아 동쪽 해역에 자생하고 있으며, 국내에서는 관상가치가 높아 정원용이나 조경용 소재로 이용되고 있는 대표적인 산림식물이다. 억새속(Miscanthus)은 물억새(M. sachariflorus), 참억새(M. sinensis), 억새아재비(M. longiberbis), 장억새(M. changii)로 분류하고 있으며, 이는 종을 구분하는 화기구조가 작고, 변이가 다양하기 때문이다. 그 중 참억새는 이러한 변이를 이용하여 다양한 초장과 엽 무늬를 가진 재배품종이 많이 개발되었으며, 내한성 또한 강해 정원용 관상식물로서의 가치가 높이 평가되고 있다(Lewandowsik et al, 1964). 지금까지 참억새 재배품종은 엽 무늬를 중심으로 개발되어 품종간 높은 유사성을 가지고 있어 구별하는데 어려움이 있었다. 본 연구에서는 새로운 참억새 품종개발시 기존품종과의 차이를 구별할 수 있는 기준을 제시하고자 기존 참억새 재배품종들에 대해 형태적 특성을 조사 분석하여 고찰해 보았다. 조사결과 가로무늬를 가진 'Strictus'와 'Zebrinus' 품종은 형태적으로 매우 유사하였으나 전체적인 모습에서 'Zebrinus'가 'Strictus' 품종에 비해 잎의 끝부분이 늘어지는 정도가 커 처진 특성을 나타내었다. 또한, 세로무늬를 가진 'Cosmopolitan'과 'Carbaret' 품종의 경우 초장 및 엽신의 길이가 유사하였으나 엽 무늬 위치에 차이가 있는 것으로 나타났다. 엽 폭이 좁은 세엽종인 'Morning Light'와 'Little Kitten' 품종은 형태적 차이는 없지만 가장자리 엽 무늬 유무로 구분이 가능하였다. 반면에 'Adagio'와 'Little Kitten, 품종은 형태적으로 매우 유사하여 좀 더 세밀한 조사가 필요할 것으로 보인다. 앞으로 더 많은 참억새 재배품종의 형태적 특성을 조사한 자료를 이용한다면 신품종 개발시 기존 재배품종과의 차이를 비교하는데 효율적으로 이용될 것으로 기대된다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.151-163
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• 1983

This study was conducted to investigate the responses to photoperiod of some Korean rice cultivars at four levels of the day length, 10, 12, 14 and 16 hours. Generally the days to heading were reduced in the short day length. Japonica varieties seemed to have shorter basic vegetative growth phase and, were more sensitive to day length, while Japonica crossed Indica varieties showed longer basic vegetative growth phase and, were less sensitive to day length. All tested varieties could be classified into 19 ecotypes based on the periods of the basic vegetative growth phase and the sensitivity to day length.