Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Development of molecular markers for varietal identification of Brassica juncea on the basis of the polymorphic sequence of ITS regions and MITE families

갓 (Brassica juncea) 품종구분을 위한 ITS 영역 및 MITE Family 정보를 이용한 분자표지 개발

  • Yang, Kiwoung (Department of Horticulture, Sunchon National University) ;
  • Yi, Go-eun (Department of Horticulture, Sunchon National University) ;
  • Robin, Arif Hasan Khan (Department of Horticulture, Sunchon National University) ;
  • Jeong, Namhee (Department of Horticulture, Sunchon National University) ;
  • Lee, Yong-Hyuk (Agricultural Technology Center of Yeosu City) ;
  • Park, Jongin (Department of Horticulture, Sunchon National University) ;
  • Kim, Hoyteak (Department of Horticulture, Sunchon National University) ;
  • Chung, Mi-Young (Department of Agricultural Education, Sunchon National Universtiy) ;
  • Nou, Ill-Sup (Department of Horticulture, Sunchon National University)
  • 양기웅 (순천대학교 생명산업과학대학 원예학과) ;
  • 이고은 (순천대학교 생명산업과학대학 원예학과) ;
  • 아리프 하산 칸 로빈 (순천대학교 생명산업과학대학 원예학과) ;
  • 정남희 (순천대학교 생명산업과학대학 원예학과) ;
  • 이용혁 (여수시 농업기술센터) ;
  • 박종인 (순천대학교 생명산업과학대학 원예학과) ;
  • 김회택 (순천대학교 생명산업과학대학 원예학과) ;
  • 정미영 (순천대학교 사범대학 농업교육과) ;
  • 노일섭 (순천대학교 생명산업과학대학 원예학과)
  • Received : 2015.11.24
  • Accepted : 2016.03.23
  • Published : 2016.04.30
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Abstract

Brassica juncea (2n = 4x = 36, AABB genome, 1,068 Mb) is a U's triangle species and an amphidiploid derivative of B. rapa and B. nigra. Fifteen varieties were used to study the ITS (internal transcribed spacer) regions of ribosomal DNA and MITEs (miniature inverted-repeat transposable elements) with a view of developing specific molecular markers. ITSs and MITEs are an excellent resource for developing DNA markers for genomics and evolutionary studies because most of them are stably inherited and present in high copy numbers. The ITS (ITS1 and ITS2) sequence was compared with the consensus sequence of B. rapa and B. nigra. Variation in ITS1 created two separate groups among 15 varieties, with 10 varieties in one group and 5 in the other. Phylogenetic analysis revealed two major clusters for those 10 and 5 varieties. Among the 160 different MITE primers used to evaluate the selected 15 varieties of B. juncea, 70 were related to the Stowaway, 79 to the Tourist, 6 to the hAT, and 5 to the Mutator super-families of MITEs. Of 160 markers examined, 32 were found to be polymorphic when fifteen different varieties of B. juncea were evaluated. The variety 'Blackgat' was different from the other mustard varieties with respect to both phenotype and genotype. The diversity of 47 additional accessions could be verified using eight selected molecular markers derived from MITE family sequences. The polymorphic markers identified in this study can be used for varietal classification, variety protection, and other breeding purposes.

갓(Brassica juncea; 2n = 4x = 36, AABB genome, 1,068Mb)은 U's triangle의 배추와 흑겨자 사이의 복이배체 작물로 구분한다. 본 연구는 갓 15 품종의 ribosomal DNA ITS 영역과 MITE를 이용하여 갓의 유연관계 및 품종구분 분자표지를 확인하였다. Ribosomal DNA ITS 영역은 종 및 품종의 유연관계를 알아보는 연구로 많이 사용되고 있어서, 이를 이용하여 갓 15 품종의 유연관계를 알아보았다. 또한, MITE는 매우 많은 copy 수를 가지고 있고, 유전적으로 안정적이기 때문에 유전체 및 진화 연구에 매우 적합한 재료이다. MITE를 이용한 갓의 품종구분 분자표지를 확인하기 위해 MITE super-families 중 Stowaway(BraSto) 관련 70점, Tourist(BraTo) 관련 79점, hAT(BrahAT) 관련 6점, Mutator(BraMu) 관련 5점으로 품종구분 표지를 알아보았다. 총 160점의 분자표지 중 32점이 갓 15 품종에서 뚜렷한 다형성을 보였다. 특히, 흑갓은 표현형뿐만 아니라 유전자형도 매우 다르게 나타났다. 또한 8점의 MITE 분자표지를 활용하여 47점의 유전자원에서 다형성 및 품종구분 표지로의 활용 가능성을 확인하였다. 이러한 다형성 표지들은 갓의 품종구분 및 품종 보호에 매우 유용하게 사용할 수 있을 것이라 기대한다.

Keywords

References

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