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Metagenomic Approach on the Eukaryotic Plankton Biodiversity in Coastal Water of Busan (Korea) (부산 연안역의 진핵플랑크톤 종다양성에 대한 메타게놈 분석 연구)

  • Yoon, Ji-Mie;Lee, Jee-Eun;Lee, Sang-Rae;Rho, Tae-Keun;Lee, Jin-Ae;Chung, Ik-Kyo;Lee, Tong-Sup
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.17 no.2
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    • pp.59-75
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    • 2012
  • The species composition of plankton is essential to understand the material and energy cycling within marine ecosystem. It also provides the useful information for understanding the properties of marine environments due to its sensitivity to the physicochemical characteristics and variability of water masses. In this study we adopted metagenomics to evaluate eukaryotic plankton species diversity from coastal waters off Busan. Characteristics of water masses at sampling sites is expected to be very complex due to the mixing of various water masses; Nakdong River runoff, Changjiang diluted water (CDW), South Sea coastal water, and Tsushima warm current. 18S rDNA clone libraries were constructed from surface waters at the three sites off Busan. Clone libraries revealed 94 unique phylotypes from 370 clones; Dinophyceae(42 phylotypes), Ciliophora(15 phylotypes), Bacillariophyta(7 phylotypes), Chlorophyta(2 phylotypes), Haptophyceae(1 phylotype), Metazoa(Arthropoda( 17 phylotypes), Chaetognatha(1 phylotypes), Cnidaria(2 phylotypes), Chordata(1 phylotype)), Rhizaria (Acantharea(2 phylotypes), Polycystinea(1 phylotype)), Telonemida(1 phylotype), Fungi(2 phylotypes). The difference in species diversity at the closely located three sites off Busan may be attributed to the various physicochemical properties of water masses at these sites by the mixture of water masses of various origins. Metagenomic study of species composition may provide useful information for understanding marine ecosystem of coastal waters with various physicochemical properties in the near feature.

Characteristics of Microbial Community Structures of the Methane Hydrate Sediments in the Ulleung Basin, East Sea of Korea (동해 울릉분지 메탄 하이드레이트 퇴적토의 미생물 군집 특성)

  • Shin, Ji-Hye;Nam, Ji-Hyun;Lee, Jin-Woo;Lee, Dong-Hun
    • Korean Journal of Microbiology
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    • v.50 no.3
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    • pp.191-200
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    • 2014
  • Gas hydrates play a significant role in the global carbon cycle and climate change because methane, a greenhouse gas, can be released from the dissociation of gas hydrate. Anaerobic oxidation of methane (AOM) is an important process that consumes more than 90% of the methane released into the hydrosphere and atmosphere. In this study, the microbial community associated with the methane gas hydrate sediment in the Ulleung basin, East Sea of Korea (UBGH) was analyzed by phylogenetic analysis of the mcrA and 16S rRNA gene libraries. A vertical stratification of the dominating anaerobic methane oxidizer (ANME)-1 group was observed at the surface and the sulfate methane transition zone (SMTZ). The ANME-2c group was found to be dominant in the high methane layer. The archaea of marine benthic group B, which is commonly observed in the AOM region, accounted for more than 50% of the identifications in all sediments. Nitrate reducing bacteria were predominant at SMTZ (Halomonas: 56.5%) and high methane layer (Achromobacter: 52.6%), while sulfate reducing bacteria were not found in UBGH sediments. These results suggest that the AOM process may be carried out by a syntrophic consortium of ANME and nitrate reducing bacteria in the gas hydrates of the Ulleung Basin of the East Sea.

Salinity Distribution in the Mid-eastern Yellow Sea during the High Discharge from the Keum River Weir (금강하구언 대량방류시 황해 중동부 해역의 염분분포)

  • Choi, Hyun-Yong;Lee, Sang-Ho;You, Kwang-Woo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.1
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    • pp.1-9
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    • 1999
  • Hydrographic survey was carried out in the mid-eastern Yellow Sea from Keum River to Taean Peninsula in order to study the motion of the freshwater from the Keum River during July 07-12, 1997 when a large volume of freshwater was discharged from the Keum River weir. The low-salinity (less than 30.0 psu) plume was distributed over the large area between the Keum River and Ochong Island, 60 km northwest off the Keum River mouth. A band of relatively low saline water, originating from the Keum River, was also observed to the north of Ochong Island. The strong haline front had advanced from near Sibidongpa Island to Ochong Island, 25 km northwest of Sibidongpa Island, for 48 hours. A northwestward flow of a speed greater than 0.2 m/s was observed in the surface plume layer to the north of Sibidongpa Island where the water column was strongly stratified. The observed mean flow and the change of the frontal position are interpreted as resulting from the spreading of the Keum River plume. These results suggest that the discharge from the Keum River plays an important role in the coastal circulation of the mid-eastern Yellow Sea adjacent to the river.

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Development of Ecologically Suitable Habitat Model for the Sustainable Sea Cucumber Aquafarm (지속가능한 해삼 양식장 조성을 위한 생태적합 서식처 모형 개발)

  • Oh, Yoon Wha;Kang, Min-Seon;Wi, Jin Hee;Lee, In Tae
    • Ecology and Resilient Infrastructure
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    • v.2 no.1
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    • pp.64-79
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    • 2015
  • We investigated the tidal current, hydrographic data, and benthic environment of major sea cucumber (Holothuroidea, de Blainville, 1834) habitats in Baengnyeongdo, Jindo and Uljin to understand the optimal environmental or ecological habitat for sea cucumbers. The three study areas were characterized by a cold-water mass of temperatures ranging $12{\sim}18^{\circ}C$, with an active circulation between the surface and deep waters. According to an analysis of the tidal current map, a strong flow velocity of $100{\sim}120cm\;s^{-1}$ appeared in Baengnyeongdo and Jindo. The three sea cucumber habitats showed the common characters of a bottom sediment composed of sand-silt, a diverse seaweed colony and benthic organisms, and boulders and rocks which provide a hideout for the organisms. We aimed to draw the optimal habitat condition for sea cucumbers in Korea, and the result showed that the low water temperature, rapid water flow, active vertical mixing between surface and deep waters, bottom composed by sand-silt, large rocks, and diverse seaweed colony and benthic organism were important factors. The optimal habitat for Juvenile sea cucumbers was the intertidal areas characterized by a muddy bottom, reef, and seaweed. The optimal habitat for adult sea cucumbers was characterized by a place where sand and mud are mixed, and the body size of the sea cucumber was proportional to water depth, and the relatively large boulders and rocks compared to the intertidal area.

THE CHARACTERISTICS OF RESIN INFILTRATED INCIPIENT CARIOUS LESIONS (Resin infiltration을 시행한 법랑질 초기 우식 병소의 특성)

  • Kim, Eun-Young;Kwon, Min-Seok;Kim, Shin;Jeong, Tae-Sung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.39 no.1
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    • pp.1-10
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    • 2012
  • The concept of resin infiltration which was born in an innovative philosophy to arrest the incipient caries. However, the structural changes of resin infiltrated lesions have not yet explained completely. The liquid resin might contribute not only to maximizing the penetration but to deteriorating physical stability. This study was performed to examine some physical and histological features of resin infiltrated incipient carious lesions. With the specimen of resin infiltrated lesions, microhardness by nanoindentation in depth profile, morphology of resin tags were revealed after HCl dissolution, and degree of microleakage were assessed. The percentage of microhardness of surface layer and lesion body of untreated specimen to sound enamel was 64.6% and 24.6% respectively, while that of resin-infiltrated lesions was 72.1%, showing significant difference (p<0.05). The resin tags observed under SEM had relatively homogeneous length of 433(282~501) ${\mu}m$ on the average. Among 20 specimens for microleakage assessment, 13 specimens showed no leakage while 5 and 2 showed leakage into outer and inner half of lesion respectively. It was thought the infiltrant resin penetrates deeply and homogeneously into lesion body and improves its hardness with relatively good physical stability.

Spatio-temporal Variability of Phytoplankton Community in the Jeju Marine Ranching Area (JMRA) (제주 바다목장 해역 식물플랑크톤 군집의 시·공간적 변동 특성)

  • Yoon, Yang Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.11
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    • pp.7761-7772
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    • 2015
  • This study describes the spatio-temporal distributions in phytoplankton community such as species composition, standing crops and dominant species from April to November 2008 in the Jeju Marine Ranching Area (JMRA). A total of 106 species of phytoplankton belonging to 63 genera was identified. In particular, diatoms and dinoflagellates were occupied more than 61% and 35% of total species, respectively. The annual dominant species were Skeletonema costatum-like species(ls) in April, Torodinium teredo, Cylindrotheca closterium, Scrippsiella trochoidea in June, S. costatum - ls, Thalassionema nitzschioides, Pseudo-nitzschia pungens, Ebria tripartita in September and Corethron pennatum, Dictyocha fibura, Neoceratium teres in November. Phytoplankton cell density ranged between $0.6cells{\cdot}mL^{-1}$ in June and $64.0cells{\cdot}mL^{-1}$ in April. It fluctuated with an annual mean of $11.7cells{\cdot}mL^{-1}$ between the lowest value of $0.9cells{\cdot}mL^{-1}$ in November and the highest value of $37.0cells{\cdot}mL^{-1}$ by S. costatum -ls in April. Diversity index in September was higher than diatom blooming seasons in April. According to the phytoplankton community structure, the biological oceanographic characteristics of the JMRA was characterized by nanoplankton during a year. That is, primary production is deemed to have a higher possibility of being adjusted by a reproduction by material cycle in the ecosystems than nutrients supply from the lands.

Clay minerals and geochemistry of continental shelf sediment around Jeju Island in the northern East China Sea (제주도 주변해역 대륙붕 퇴적물의 지화학적 조성과 점토광물 연구)

  • Youn, Jeung-Su
    • The Korean Journal of Quaternary Research
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    • v.23 no.1
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    • pp.25-37
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    • 2009
  • Geochemical composition and clay minerals of surface and core sediments around off the Jeju Island were analyzed for identification of sediment origins. The clay mineral distribution is mainly controlled by the sediment source and the dominant circulation pattern. Smectite is highly concentrated (>8%) in the northwest near the South Yellow Sea and in the outer-shelf mud patch. It seems to be due to the high supply of smectite transported from China where fine-grained sediments are discharged from modern and ancient Huanghe River. The relatively high abundance of kaolinite are found in northeastern nearshore area and the southwest near Changjiang estuary. It seems to be supplied from Changjiang River and the southwestern Korea rivers. The sediment accumulation rates measured by $^{210}Pb$ geochronrom mowere 0.20 to 0.54cm/mr or 0.15 to $0.42g/cm^2{\cdot}mr^{-1}$ AOJI, with decreasing rates from the west part to the east part, resulting in the supply of fine-grained suspended sediments from the Changjiang and Huanghe Rivers system. The discrimination diagrams clearly show that the sediments around Jeju Island in the northern East China Sea are ultimately sourced from Chinese rivers, especially from the Huanghe River, whereas the sediment in the northeast part might come from Korean rivers and the Jeju Island.

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Estimation of Benthic Microalgae Chlorophyll-a Concentration in Mudflat Surfaces of Geunso Bay Using Ground-based Hyperspectral Data (지상 초분광자료를 이용한 근소만 갯벌표층에서 저서성 미세조류의 엽록소-a 공간분포 추정)

  • Koh, Sooyoon;Noh, Jaehoon;Baek, Seungil;Lee, Howon;Won, Jongseok;Kim, Wonkook
    • Korean Journal of Remote Sensing
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    • v.37 no.5_1
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    • pp.1111-1124
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    • 2021
  • Mudflats are crucial for understanding the ecological structure and biological function of coastal ecosystem because of its high primary production by microalgae. There have been many studies on measuring primary productivity of tidal flats for the estimation of organic carbon abundance, but it is relatively recent that optical remote sensing technique, particularly hyperspectral sensing, was used for it. This study investigates hyperspectral sensing of chlorophyll concentration on a tidal flat surface, which is a key variable in deriving primary productivity. The study site is a mudflat in Geunso bay, South Korea and field campaigns were conducted at ebb tide in April and June 2021. Hyperspectral reflectance of the mudflat surfaces was measured with two types of hyperspectral sensors; TriOS RAMSES (directionalsensor) and the Specim-IQ (camera sensor), and Normal Differenced Vegetation Index (NDVI) and Contiuum Removal Depth (CRD) were used to estimate Chl-a from the optical measurements. The validation performed against independent field measurements of Chl-a showed that both CRD and NDVI can retrieve surface Chl-a with R2 around 0.7 for the Chl-a range of 0~150 mg/m2 tested in this study.

Acoustic Doppler Current Profiler Bottom Tracking Survey of Flow Structures around Geumo Archipelago in the Southern Waters of Korea (ADCP bottom tracking에 의한 금오열도 주변의 해수유동)

  • Choo, Hyo-Sang
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.5
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    • pp.589-600
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    • 2019
  • In order to investigate the flow structures around Geumo archipelago on Southern Waters of Korea, water movements were measured for 25 hours during spring tide in May and neap tide in September 2002 using ADCP (Acoustic Doppler Current Profiler) attached to a running boat. Dominant directions of ebb and flood current at spring tide are SE-NW, representing the average flow rate of approximately 40cm/s in the surface layer. However because of the topographical reason, the direction and speed of the flow in the narrow waterway sea area around the northwest of Gae Island were different. There was no notable baroclinic component of tidal flow at spring tide. This indicates that the sea area has been actively engaged in vertical mixing due to island wake or eddy due to narrow waterways, shallow water depth and rapid flow rate around archipelago. At neap tide, dominant directions of tidal flows are SSE-NNW and the average flow rate in the surface layer is about 85 percent of the spring tide. The duration and intensity of the flow direction are shorter and less dominant than the spring tide. It is expected that asymmetrical tidal mixing will occur due to vertical velocity shear and horizontal eddies. From daily mean tidal flows obtained from the ADCP observation, it was found that the northwest of Gae Island have flows in NW~NE, the west of Geumo Island have the average currents of up to 21 cm/s WSW~SSW and counterclockwise circulation or eddy currents are formed in the west of Sori Island.

Spatial Distribution of Extremely Low Sea-Surface Temperature in the Global Ocean and Analysis of Data Visualization in Earth Science Textbooks (전구 대양의 극저 해수면온도 공간 분포와 지구과학교과서 데이터 시각화 분석)

  • Park, Kyung-Ae;Son, Yu-Mi
    • Journal of the Korean earth science society
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    • v.41 no.6
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    • pp.599-616
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    • 2020
  • Sea-surface temperature (SST) is one of the most important oceanic variables for understanding air-sea interactions, heat flux variations, and oceanic circulation in the global ocean. Extremely low SSTs from 0℃ down to -2℃ should be more important than other normal temperatures because of their notable roles in inducing and regulating global climate and environmental changes. To understand the temporal and spatial variability of such extremely low SSTs in the global ocean, the long-term SST climatology was calculated using the daily SST database of satellites observed for the period from 1982 to 2018. In addition, the locations of regions with extremely low surface temperatures of less than 0℃ and monthly variations of isothermal lines of 0℃ were investigated using World Ocean Atlas (WOA) climatology based on in-situ oceanic measurements. As a result, extremely low temperatures occupied considerable areas in polar regions such as the Arctic Ocean and Antarctic Ocean, and marginal seas at high latitudes. Six earth science textbooks were analyzed to investigate how these extremely low temperatures were visualized. In most textbooks, illustrations of SSTs began not from extremely low temperatures below 0℃ but from a relatively high temperature of 0℃ or higher, which prevented students from understanding of concepts and roles of the low SSTs. As data visualization is one of the key elements of data literacy, illustrations of the textbooks should be improved to ensure that SST data are adequately visualized in the textbooks. This study emphasized that oceanic literacy and data literacy could be cultivated and strengthened simultaneously through visualizations of oceanic big data by using satellite SST data and oceanic in-situ measurements.