• Journal of Nutrition and Health
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• v.12 no.2
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• pp.87-93
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• 1979

인구적 증가와 사료 가격의 상승으로 인한 육류 제품의 가격이 상승하고 있음은 우리나라를 비롯하여 세계적인 현황이다. 이 문제에 대처하는 한 방법으로 새로운 단백질 급원으로써 Single Cell Protein이 개발되었고 이에 대한 여러가지 연구가 되어지고 있다. Single Cell Protein이 인간에게 식용이 될 동물이나 나아가서는 인간에게 직접 식품으로 사용되기 전에 인체나 동물 체내에 끼칠 영향과 그 안전성을 확인하려는 하나의 노력으로 이 연구가 시도되었다. 동물 체내 대사에 미치는 SCP의 악영향의 주원인은 함유된 독성물질로 보고 그 독성물질이 SCP 생산 및 처리 과정 중 어느 시기에 생겨나는가를 규명해 보고자 하였다. Fermentation을 끝낸 SCP의 Supernatant내에 함유된 Inorganic phosphate(I.P.)의 함량을 측정하여 SCP세포의 Viability를 알아보았다. Fermentation을 끝낸 후 10일까지는 I.P.가 차츰줄다가 40일이 지났을 때에는 I.P.의 증가를 보였으나 직후보다는 낮음을 나타냈다. 또한 냉동 건조시킨 뒤에도 SCP Cell이 살아있음을 보였다. 또한 위와 관련지어서 SCP를 Protein급원으로 써서 사육한 흰쥐에 나타난 Protein Efficiency Ratio(PER)를 통하며 SCP의 질을 평가하였다. 실험결과로 나타난 것을 보면 표준 식이에 사용된 Casein의 PER$(\overline{X}=2.58)$이 SCP의 PER$(Dict 1;\overline{X}=0.888$, $Dict 2;\overline{X}=0.893$, $Dict 3;\overline{X}=0.860)$ 보다 유의적으로 높았다. 그리고 총실험기간의 평균치를 볼 때, Fermentation을 끝내고 3일 후와 6일 후에 냉동 건조시킨 SCP의 PER은 시판되는 SCP의 것과 별다른 차이를 보이지 않았다. 그리나 후반기에 있어서는 Fermentation을 끝내고 3일과 6일 후에 냉동 건조시킨 SCP의 PER이 시판되는 SCP의 것보다 약간 저조함을 보였다. 그리고 Casein의 PER은 총 사육기간은 통하여 별 변동이 없음에 반하여 후반기에 SCP의 PER이 급격한 저하를 보임은 SCP 사용상의 문제점을 나타낸 것으로 해석 된다.

• Korean Journal of Environmental Biology
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• v.42 no.1
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• pp.54-61
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• 2024

The ecosystem provides a diverse array of environmental conditions for organisms, and only those that are capable of successfully adapting to these conditions within their habitats can endure, thrive, and proliferate. Further, the environmental conditions within these habitats can significantly affect the bioavailability of chemicals that are introduced therein, thus resulting in varied adverse impacts on the organisms. The present study aims to evaluate the sensitivity of Yuukianura szeptyckii - a species adapted to riparian - to heavy metals following ISO guideline 11276, with the objective of assessing its potential as an indicator species for ecotoxicological evaluations in riparian habitats. The findings revealed that cadmium and copper both had significant toxic effects depending on their concentrations. For cadmium, the LC₅₀ was 280 mg kg^-1, EC₅₀ was 66 mg kg^-1, and NOEC and LOEC were 25 and 50 mg kg^-1, respectively. For copper, the LC₅₀ was 911 mg kg^-1, EC₅₀ was 151 mg kg^-1, and LOEC was 50 mg kg^-1. Comparative analysis with previous results for the international standard species Folsomia candida and the domestic standard species Allonychiurus kimi indicated that Y. szeptyckii exhibited even greater sensitivity to toxicity values. The adverse effects on survival and reproduction were closely associated with the influx concentration of heavy metals in their bodies. Altogether, the results suggest that Y. szeptyckii is a sensitive species for ecotoxicological assessments in riparian habitats, thus making it suitable as an indicator species, particularly in riparian ecosystems that are characterized by relatively high humidity conditions.

• Journal of Dairy Science and Biotechnology
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• v.34 no.2
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• pp.99-116
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• 2016

We herein performed animal safety assessment in accordance with Good Laboratory Practice (GLP) regulations with the aim of developing sialic acid from glycomacropeptide (hereafter referred to as "GMP") as an index ingredient and functional component in functional foods. GMP is a type of whey protein derived from milk and a safe food, with multiple functions, such as antiviral activity. A test substance was produced containing 7% (w/w) sialic acid and mostly-hydrolyzed whey protein (hereafter referred to as "7%-GNANA") by enzymatic treatment of substrate GMP. The maximum intake test dose level was selected based on 5,000 mg/kg/day dose set for male NOEL (no-observed-effect-level) and female NOAEL (no-observed-adverse-effect-level) determined by a dose-range finding (DRF) test (GLP Center of Catholic University of Daegu, Report No. 15-NREO-001) that was previously conducted with the same test substance. To evaluate the toxicity of a repeated oral dose of the test substance in connection with the previous DRF study, 1,250, 2,500, and 5,000 mg/kg of the substance were administered by a probe into the stomachs of 6-week-old SPF Sprague-Dawley male and female rats for 90 d. Each test group consisted of 10 male and 10 female rats. To determine the toxicity index, all parameters, such as observation of common signs; measurements of body weight and food consumption; ophthalmic examination; urinalysis, electrolyte, hematological, and serum biochemical examination; measurement of organ weights during autopsy; and visual and histopathological examinations were conducted according to GLP standards. After evaluating the results based on the test toxicity assessment criteria, it was determined that NOAEL of the test substance, 7%-GNANA, was 5,000 mg/kg/day, for both male and female rats. No animal death was noted in any of the test groups, including the control group, during the study period, and there was no significant difference associated with test substance, as compared with the control group, with respect to general symptoms, body weight changes, food consumption, ophthalmic examination, urinalysis, hematological and serum biochemical examination, and electrolyte and blood coagulation tests during the administration period (P<0.05). As assessed by the effects of the test substance on organ weights, food consumption, autopsy, and histopathological safety, change in kidney weight as an indicator of male NOAEL revealed up to 20% kidney weight increase in the high-dose group (5,000 mg/kg/day) compared with the change in the control group. However, it was concluded that this effect of the test substance was minor. In the case of female rats, reduction of food consumption, increase of kidney weight, and decrease of thymus weight were observed in the high-dose group. The kidney weight increased by 10.2% (left) and 8.9% (right) in the high-dose group, with a slight dose-dependency compared with that of the control group. It was observed that the thymus weight decreased by 25.3% in the high-dose group, but it was a minor test substance-associated effect. During the autopsy, botryoid tumor was detected on the ribs of one subject in the high-dose group, but we concluded that the tumor has been caused by a naturally occurring (non-test) substance. Histopathological examination revealed lesions on the kidney, liver, spleen, and other organs in the low-dose test group. Since these lesions were considered a separate phenomenon, or naturally occurring and associated with aging, it was checked whether any target organ showed clear symptoms caused by the test substance. In conclusion, different concentrations of the test substance were fed to rats and, consequently, it was verified that only a minor effect was associated with the test substance in the high-dose (5,000 mg/kg/day) group of both male and female rats, without any other significant effects associated with the test substance. Therefore, it was concluded that NOAEL of 7%-GNANA (product name: Helicobactrol) with male and female rats as test animals was 5,000 mg/kg/day, and it thus was determined that the substance is safe for the ultimate use as an ingredient of health functional foods.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.2
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• pp.147-155
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• 2008

A series of experiments were conducted to find standard test organisms and to develop test protocols for sediment toxicity tests using indigenous amphipods inhabited in Korean coastal environments. The indigenous amphipods Mandibulophoxus mai and Monocorophium acherusicum were well associated with various sediment substrates from sand to mud. The tolerance limits to various physico-chemical factors affecting bioassay results such as temperature, salinity and total ammonium as well as the sensitivities to contaminants in water and sediments were investigated using M. mai and M. acherusicum in the present study. These amphipods were tolerable to the adequate ranges of salinity ($10{\sim}30\;psu$), temperature ($10{\sim}25^{\circ}C$) and ammonia (<50 ppm). They have relevant sensitivities to the reference toxicants, dissolved cadmium as well as other metals and organic pollutants, when compared to the standard test species used in other countries. Field-sediment toxicity tests revealed that M. mai would be more sensitive to sediment-associated pollutants than M. acherusicum, while the sensitivity of M. acherusicum was comparable to those of other sediment test species in other countries. Overall results of this first attempt to develop an amphipod sediment toxicity test protocol in Korea indicated that M. mai and M. acherusicum should be applicable in the toxicity assessment of contaminated sediments, following the further evaluation encompassing various ecological and toxicological evaluation and the standardization of test method.

• The Korean Journal of Pesticide Science
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• v.14 no.1
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• pp.10-15
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• 2010

Most pesticides are poorly soluble in water and must be dissolved in a solvent carrier before being added to the test medium on aquatic toxicity test. The concentration of solvent is critical to the success of a test. This study were conducted to recommend possible organic solvents which have good solubility for pesticides and low toxicity to Korean native water flea to establish new standard toxicity test methods using Korean native water flea for ecological risk assessment of pesticide. Four Korean freshwater cladocerans, Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocephalus vetulus were exposed to 10 different organic solvents during 48 hours to evaluate their toxic response to solvents. Ethyl acetate was the most toxic to cladocerans tested. Although ethyl ether was the least toxic to cladocerans tested, it may not adequate as possible solvent in aquatic toxicity test due to high volatility and low water solubility. In conclusion, acetone, methanol, ethanol and acetonitrile which has low toxicity as well as good water solubility are recommended as optimal organic solvent to use in aquatic toxicity tests with Korean native cladocerans tested.

• Proceedings of the KSEEG Conference
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• 2003.04a
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• pp.258-264
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• 2003

중력이상 및 수치고도모델을 이용하여 한반도 모호면 심도를 추출하였다 중력이상값은 인공위성고도레이더 관폭값을 주로 이용한 전지구 모델을 이용하여 데이터영역 뿐 만 아니라 주파수영역에서도 자료의 균질성을 확보하였다. 모호면 추출은 Kim et al. [2000a]에 의해 제안된 스펙트럼 대비법 및 후리에급수를 이용한 파워스펙트럼분석법을 이용하였다. 전자는 지각근형을 전제로, 지형에 의한 중력효과와 후리에어 중력이상을 파동수영역에서 대비하여 모호면의 심도를 계산하는 방법이고, 후자는 완전부우게 중력이상으로부터 푸리에변환을 이용하여 지하 밀도 변화층의 심도를 계산하는 방법이다. 이 두 모호면은 서로 0.53의 상관관계를 갖고 있으며, 이는 모호면 산출의 방법론적인 차이 및 계산상의 오차인 것으로 사료된다. 이렇게 두 가지 독립적인 방법으로 추출된 모호면을 하나로 통합하기 위한 한 방법으로, 두 모호면의 차이를 계산한 후, 이를 최소자승법을 이용, 두 모호면을 보정하였다. 결과적으로 한반도의 최종 모호면의 평균심도는 32.0km, 표준편차는 2.5km 이며, 최소, 최대 심도는 20.3, 36.6km으로 나타났다. 이 경우 지형에 의한 중력효과는 스펙트럼대비법에 의해 제거된 결과이나, 한반도의 지각이 완전한 지각판 내에 놓여 있어서 Airy-Heiskanin 지각균형설의 가정이 타당성이 있는가, 혹은 국부적인 응력장에 의해 한반도의 지각이 과연 얼마나 지지되고 있는가 하는 것에 대한 추가적인 연구가 필요하며, 이에 앞서, 일정한 밀도차를 갖는 연속적인 밀도변화층이 존재한다는 가정이 반드시 필요하다.에는 관련성을 갖고 있으며, 이는 유류 분해정도를 파악하는 지시자로써 특정 무기 오염물질을 이용할 수 있을 가능성이 있으므로 좀더 이들 관계성에 대한 연구가 진행될 필요성이 있다고 판단된다.고 과학적으로 분석할 수 있는 방법이 될 수 있을 것으로 기대된다. 의미를 되새기는 것으로 짧은 연구를 시작하겠다. 등은 활성 값이 70% 이상으로 퇴적물 독성이 상대적으로 낮았다. 이중나선 DNA 함량은 28.4 % - 49%로 대조군에 비해서 감소가 크다. 대부분의 정점이 대조군의 30% 내외로 정점 간의 차이는 크지는 않다. 그러나 다른 측정자료와 같이 정점 22에서 18%로 최소치를 나타내고, 정점 2, 12에서 20% 내외의 값을 보인다. 종합적으로 볼 때 오염물질의 유입이 크고, 광양제철 인근 정점 들이 모두 다른 정점에 비해서 낮아서, 퇴적물 독성이 높은 정점으로 조사되었다.hiwo의 광합성 능력은 낮은 농도들에서는 대조구와 유사하였으나, 5 $\mu\textrm{g}$/l의 높은 농도에서는 초기에 매우 낮은 광합성 능력을 보이다가 시간이 경과하면서 대조군보다 더 높은 경향을 나타냈다. 이러한 결과는 식물플랑크톤이 benso[a]pyrene의 낮은 농도에서 노출될 때는 이 물질을 탄소원으로 사용할 가능성이 있음을 시사한다. 본 연구의 결과들은 연안해역에 benso[a]pyrene과 같은 지속성 유기오염물질이 유입되었을 때 내정여부에 따라 식물플랑크톤 군집내 종 천이와 일차생산력에 크게 영향을 미칠 수 있음을 시사한다.TEX>5.2개)였으며, 등급별 회수율은 각각 GI(8.5%), GII

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.703-712
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• 2021

In this study, a formula was derived to calculate the damage impact distance using the Chemical Accident Response Information System (CARIS) so that local governments can decide on the evacuation and notification of 13 types of substances. The National Institute of Chemical Safety selected 16 out of 97 types of accident preparedness substances in 2018 and called them residents' evacuation preparedness substances. In a chemical accident, local governments should prepare for resident notification, such as emergency disaster texts. Using the CARIS in Chungju, this study modeled the damage-affected distances of 13 types of substances for the evacuation of residents. Under all conditions, the coefficient of determination R2 was 0.99 or higher, representing a range of at least 0.9921 to a maximum 0.9999. The relative standard deviation between the damage impact distance obtained using the calculation formula, and the CARIS result was compared. The minimum separation distance was corrected considering the actual chemical accident response situation, and the range was found to be between 0.58 and 5.97%. The damage impact distance can be calculated at the site using the calculation formula derived from the research, and local governments can determine whether to evacuate or notify residents.

• Analytical Science and Technology
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• v.31 no.6
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• pp.225-231
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• 2018

Benzoic acid, methyl paraben, and butyl paraben are preservatives that have been used in pharmaceutical, cosmetic, and food products. However, as their toxicities for human have been reported, many nations and organizations including Korea have established a regulation limit for thier usage of these preservatives in food products. The present study developed the isotope dilution liquid chromatography mass spectrometry method for accurate determination of three target preseratives in soysauce. In this study, the isotope dilution liquid chromatography mass spectrometry method was developed for accurate determination of three target preservatives in soy sauce. LC separation was optimized considering the pKa of benzoic acid which is lower than those of methyl and butyl parabens. A C18 column was used with 5 mM ammonium acetate and methanol as mobile phases. Mass spectrometry was operated in negative mode and selected reaction monitoring mode (SRM). Soy sauce sample was cleaned-up with C18 SPE cartridge for removing matrix inferences and color material. Optimized conditions and the method were validated with soy sauce reference materials for the analysis of food preservatives from Health Science Authority in Singapore. The measured values of benzoic acid, methyl and butyl paraben agreed well with reference values within their uncertainties.

• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.250-254
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• 2007

Bacillus thringiensis strain 108 was isolated from soil and had nematicidal activity against second stage juvenile of plant root-knot nematode, Meloidogyne incognita. The strain 108, a rod shape, spore forming and Gram positive bacterium, produced lecithinase, catalase, and ${\delta}$-endotoxin. The strain 108 belongs to H serotype 3, Bacillus thuringiensis var. kurstaki. A nematicidal substance of the strain 108 was partially purified on Sephadex G-25 gel filtration, activated carbon adsorption, silica gel adsorption, and Sephadex G-10 gel filtration. $LC_{90}$ of the partially purified substance against M incognita was $1.2\;{\mu}g/ml$. The nematicidal substance was stable by heat treatment at $100^{\circ}C$ for 1hr, but was perfectly lost nematicidal activity after autoclave ($110^{\circ}C$, 30 min).

• Journal of Korean Society of Environmental Engineers
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• v.34 no.5
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• pp.316-325
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• 2012

In this study, we suggested adequate control technology by analyzing emission process and main chemical of VOC/HAPs generated from four paint factories in Busan area. And we estimated whether Kapok fiber, which is a natural material, can be commercially used for an absorbent by testing adsorption ability. As a result of this sturdy, above 60% of VOC/HAPs was volatilized and dispersed inside the working place without conducting control system of facilities during manufacturing paints. Concentration profile of VOC/HAPs, which is volatile naturally outside the factories, is surveyed above 70% at Toluene, Ethylbenzene, and Xylene. And a result of evaluation of odor attribution level about the component whose Odor Threshold is known, it is estimated that major cause material of A, B, C factories is Toluene and that of D factory is m/p-Xylene. And that result presented design arguments such as facilities specifications, activated carbon filling volume, and replace cycle of activated carbon as control technology. Also, that result presented emission process improvement such as adsorption of central-controlled ventilation device, installation of inlet flenge, and potable cleaning process. The rate of pollutant adsorption of Kapok fiber, which is natural material, is indicated about 91.9%, 66.7%. That result validated the possibility as replacement of activated carbon.