• Title/Summary/Keyword: 포장저항성

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CGMMV Tolerance Test of CGMMV-CP Trangenic Watermelon Rootstock and Establishment of Transgenic Line (CGMMV-CP 형질전환 수박대목의 CGMMV 내성시험 및 계통확보)

  • Park, Sang-Mi;Kwon, Jung-Hee;Lim, Mi-Young;Shin, Yoon-Sup;Her, Nam-Han;Lee, Jang-Ha;Ryu, Ki-Hyun;Harn, Chee-Hark
    • Journal of Plant Biotechnology
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    • v.34 no.1
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    • pp.11-17
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    • 2007
  • Previously developed transgenic watermelon rootstocks (gongdae) inserted by CGMMV-CP were examined to test the virus tolerance levels. In the restricted plastic house, the $T_{3}$ watermelon rootstock showed tolerance to CGMMV until 70 days after inoculation on the leaves while the non-transformed watermelon rootstock became susceptible at 20 days after inoculation. In the field, tolerance efficiency of transgenic rootstocks maintained up to 40% at 71 days after contamination with CGMMV in the soil while all of the non-transformed rootstocks became susceptible at 37 days with the same condition. In the same field, transgenic rootstocks showed more tolerance to CGMMV than the non-transformed rootstocks as those were inoculated on the leaves, but it showed only 10 days delay before being susceptible. Therefore, transgenic rootstocks have a characteristic of delay effect against CGMMV susceptibility, rather than resistance character. From $T_{3}$ rootstocks homozygous for the CGMMV-CP horticulturally favorable individuals were selected for further breeding and a transgenic line was finally obtained at the $BC_{1}T_{5}$. A material transfer experiment was conducted to find out if the DNA, RNA or expressed protein in the transgenic rootstocks could move to the grafted scion (non-transformed watermelon, Super-Kumcheon). PCR, northern, and western blot analysis were performed and no evidence of transferring of those materials from rootstock to scion was ever found.

A Study on the Performance Comparison of the Agents for Asphalt Pavement Recycling (재생 아스팔트 첨가제의 성능비교 연구)

  • Kim, In-Soo;Kang, Min-Soo;Suh, Young-Chan;Lee, Bong-Won
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.4
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    • pp.82-88
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    • 2012
  • The usage of recycling agent is the most effective way of enhancing the performance of recycled asphalt pavement. Generally, many countries accepted these recycling agents retarding the various types of cracks resulted in binder aging. Contrary to such general tendency, recycling agents are used as very small amount in domestic recycling plant. The main reason of this is relatively high price of recycling agent. Even though agent price is high, agent can be a effective and economical way of alternative in asphalt recycling. In this study, penetration and softning point test performed by using RTFO(Rolling Thin Film Oven) aged binder. And, PG(Performance Grade) at high temperature, fatigue and MSCR(Multi Stress Creep and Recovery) tests exercised. The oil type agent is worked well to retrieving target penetration number. In PG test, $G^*/sin{\delta}$ of agents identically showed high value and polymer type agent is good at recovery value.

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Basic Study on Development of Forest Road Pavement Using Eco-Friendly Method (친환경 임도포장공법 개발을 위한 기초연구)

  • Oh, Sewook;Lee, Gilho;Kim, Donggeun
    • Journal of the Korean GEO-environmental Society
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    • v.14 no.6
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    • pp.31-38
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    • 2013
  • This study carried out fundamental study on the forest road pavement method of cementing the ground using the compaction equipment after laying by mixing with eco-friendly stabilizer, natural soil and water. Target strength of pavement was set to 2.0MPa and the specimen was produced per mixing ratio of cement, kinds of natural soil and curing period to evaluate the durability and unconfined compressive strength. Unconfined compressive strength test was conducted to compare strength by producing the test specimen mixing environment-friendly cement as well as the test specimen mixing cement with the same mixing ratio. To evaluate the durability, surface abrasion test and water flow resistance test were conducted. In addition, SB and GB tests were conducted using iron marble and golf ball to evaluate the walking satisfaction since it can be used by visitors due to the feature of forest road.

Studies on Economic Damage of Korean Rice Pests (벼해충의 경제적 피해에 관한 연구)

  • Catling H. D.;Lee S. C.
    • Korean journal of applied entomology
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    • v.16 no.2 s.31
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    • pp.79-86
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    • 1977
  • Four experiments were carried out under farmer's field conditions to determine economic threshold levels of major rice pests aad attempt a reduction in the number of insecticide applications. In the experiments were included check treatments, insecticide schedules representing the official recommendations to farmers, and several corrective treatments. A careful record was kept of insect pest densities and disease incidence. i) In the north at Suweon and Icheon where Chilo suppresalis. (Walk.), the striped rice borer, was active in the first generation, no significant yield differences were obtained between plots receiving several insecticide applications and those left totally unprotected. The mean yields were high (5.2-7.6t/ha). ii) First generation borer activity rising to $8.6\%$ injured tillers was below the economic threshold since no yield reduction was recorded in either japonica varieties or the high-yielding Tongil variety. iii) Evidence was obtained thst berer damage was made good by replacement of infested tillers (compensatory growth), C. suppressalis populations were always low in the second generation. iv) The experimental results obtained at Suweon and Icheon do not justify the present official recommendations of 6-7 pesticide applications. v) further south at Iri a substantial yield reduction occurred due to an early outbreak of stripe disease transmitted by Laodelphax striatellus (Fallen), the small brown planthopper; a mean of 1-2 individuals/hill was recorded immediately after transplanting. C. suppressalis probably contributed to this yield reduction. vi) Several applications against the vector failed to prevent the rapid spread of stripe to the susceptible variety in the Iri experiment: in surrounding fields the resistant Tonsil varivety was ralatively unaffected. vii) Pests of lesser importance were Nephotettix cinctieps (Uhler), Nilaparvata lugens (Stil), Sogatella furcifera (Horv..), and leaf miners.

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Evaluation of Structural Stability of Plastic Greenhouses with Steel Spiral Piles on Reclaimed Lands (간척지에서 강재 나선말뚝기초를 적용한 플라스틱 온실의 안전성 평가)

  • Yum, Sung Hyun;Lee, Won Bok
    • Journal of Bio-Environment Control
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    • v.26 no.1
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    • pp.27-34
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    • 2017
  • This study was carried out to estimate structural stabilities in respect of ground footings of plastic greenhouses on reclaimed lands. A 6m-wide multi-span plastic greenhouse with steel spiral piles as well as two 8.2m-wide single-span greenhouses with steel spiral piles and continuous pipe foundation respectively were built up on a reclaimed land with a SPT N-Value of 2 and measured how much the greenhouses were lifted up and subsided. In addition, the uplift capacity of three kinds of spiral piles(${\phi}50$, ${\phi}75$ and ${\phi}100$) was determined on a nearby reclaimed land. The results showed that the greenhouses with spiral piles had a slight vertical displacement like moving up and down but the scales of the rising up and sinking were negligible when compared to that of the greenhouses. The vertical displacement of the multi-span greenhouse ranged from +9.0mm(uplift) to -11.5mm(subsidence). As for the single-span greenhouses with spiral piles and continuous pipe foundation, the measurements showed that it varied from +1.3mm to -7.7mm and from +0.9mm to -11.2mm, respectively. The allowable uplift capacity of spiral piles could all be determined under criteria of ultimate load and accordingly had a value of 0.40kN, 1.0kN and 2.5kN, respectively. It was not entirely certain enough to make a final judgement on structural stabilities in respect of ground footings, it appeared likely however that the greenhouses with steel spiral piles was tentatively observed without any problems on reclaimed lands within the period.

Assessment of the Persistence of DNA in Decomposing Leaves of CMVP0-CP Transgenic Chili Pepper in the Field Conditions (포장 조건에서 CMVP0-CP 형질전환 고추 도입유전자의 지속성 조사)

  • Lee, Bum-Kyu;Kim, Chang-Gi;Park, Ji-Young;Park, Kee-Woong;Yi, Hoon-Bok;Harn, Chee-Hark;Kim, Hwan-Mook
    • Korean Journal of Environmental Agriculture
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    • v.26 no.4
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    • pp.319-324
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    • 2007
  • This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.

Influences of Nitrogen and Potassium Rates on the Chemical and Physical Properties of Cured Leaf on Burley Tobacco (질소 및 가리 시용량이 버어리종 잎담배의 성분 및 물리성에 미치는 영향)

  • 김상범
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.1
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    • pp.1-6
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    • 1991
  • A field experiment was conducted to find out the effects of nitrogen and potassium rates on the chemical constituents and physical properties of burley tobacco (1988; Burley 21. 1989; Burley 21 and KB 101) during successive two years. KB 101 was low in total nitrogen and total alkaloid contents and potassium/total nitrogen ratio of cured leaf. but high in the lightness, red and yellow color and filling power, comparing to Burley 21. The increasing rates of total nitrogen and total alkaloid contents were larger when nitrogen applied to 22.75kg/10a, but they were smaller when nitrogen applied above 22.75 kg/l0a. The potassium content, potassium/total nitrogen ratio, filling power and shatter resistance index of cured leaf were decreased by nitrogen fertilizer addition. The potassium content and shatter resistance index were increased by potassium fertilizer addition. The effect of nitrogen rate on the potassium/total nitrogen ratio of cured leaf was larger than the effect of potassium rate. When the nitrogen fertilizer being applied above 22.75 kg/l0a, there were no advantageous effects on the chemical and physical properties. It is considered to be sufficient that the potassium application is about 35.0 kg/l0a.

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Responses of Growth Characteristics of Soybean [Glycine max (L.) Merr.] Cultivars to Riptortus clavatus Thunberg (Hemiptera: Alydidae) (콩 톱다리개미허리노린재 피해와 관련형질의 품종간 차이)

  • Oh, Young-Jin;Cho, Sang-Kyun;Kim, Kyong-Ho;Paik, Chae-Hoon;Cho, Youngkoo;Kim, Hong-Sik;Kim, Tae-Soo
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.488-495
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    • 2009
  • In recent years, an increasing amount of research has been conducted on the yield loss of soybean crop caused by Riptortus clavatus, a serious pest of legumes. The objective of this study were to examine crop damage caused by various population densities of R. clavatus and determine the association of unique growth and seed characteristics with crop damage rate in conventional soybean fields. Major growth responses to R. clavatus were examined. The cultivars that were not as seriously damaged by R. clavatus were Pungsannamulkong, Sobaegknamulkong, Duyoukong, and Sinpaldal 2, but Myeongjunamulkong, Mallikong, and Hwangkeumkong were significantly (P<0.05) damaged. The rate of seed damage caused by R. clavatus was 8.2% in a group of brown colored pubescence, while the rate was 13.0% in a group of the gray colored. Crop damage by R. clavatus clearly more reduced in cultivars with dark hilum colors including dark brown, brown, and grayish brown than those with yellow hilum colors. Concerning pod colors, crop damage was less significant in cultivars with dark brown pod color than with the light brown. Cultivars with short pod length (4.0-4.5 cm) at full seed (R6) stage showed lower seed damage rates by R. clavatus than those with long pod length (>5.6 cm). Therefore, characteristics of hilum, pod, pubescence colors, and pod length were significantly related and linked to the cultivar resistance to R. clavatus.

Engineering Characteristic of High Density Expansion Materials for Structure Restoration Technology (기초침하복원을 위한 급속 팽창재료의 공학적 특성에 관한 연구)

  • Shin, Eun-Chul;Cha, Yong-In
    • Journal of the Korean Geosynthetics Society
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    • v.7 no.2
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    • pp.1-5
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    • 2008
  • The differential settlement on ordinary concrete buildings and paved roads are often occurred and which caused the failure of structure. The grouting method can be used for correcting the settlement of the structure. However, the grouting method has a disadvantage like that it takes a long time period to get a desired strength, and it is not a continuous in the phase of reinforced effect. In this paper, as an injecting material called GPCON to complement disadvantage, it is estimated about the characteristic that has a high-density expansion. With the changing of ground conditions and amount of injection, the change of physical strength on compression, the stability against chemical material are studied through the filming of SEM. The physical strength with compression is developed to high strength due to mixing with other material. It is not react with most of the material on chemical conditions except the component of alcohol. Through the SEM test. it is confirmed that the strength of material was increased as formation is being densified.

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Evaluation of Rutting Behavior of Hot Mix Asphalt using Slag and Waste Foundry Sand as Asphalt Paving Materials (슬래그와 폐주물사를 이용한 아스팔트 혼합물의 소성변형특성에 관한 연구)

  • Lee, Kwan-Ho;Cho, Jae-Yoon;Jeon, Joo-Yong
    • 한국도로학회:학술대회논문집
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    • 2002.10a
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    • pp.89-92
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    • 2002
  • The objective of this research is to evaluate engineering properties of recycled aggregates, slag as coarse & fine aggregate and waste foundry sand(WFS) as fine aggregate, in hot mix asphalt(HMA). In this research, soundness, gradation and particle analysis, abrasion, specific gravity and absorption test were carried out. The optimum asphalt binder content(OAC) for various HMA combinations of recycled aggregate was determined by Marshall Mix Design. The ranges determined is between 7.2% and 7.5%. Indirect tensile test, resilient modulus test, creep test were carried out for characterization of rutting behavior of various combination of HMA. Judging from the limited tests, the HMA with recycled aggregates is not as good rutting resistance as the HMA with common aggregates. After finishing the Wheel tracking test, the application or feasibility for the use of recycled aggregate as asphalt paving material will be determined.

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