• Korean journal of applied entomology
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• v.25 no.2 s.67
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• pp.63-70
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• 1986

Severity of incidence of white rot on apple fruit ranged from 5 to 16% and averaged 9% over major apple growing area in 1981. An isolate of Botryosphaeria ribis obtained from rotted apples developed lesions on leaves, branches and fruits of apple, pear, peach and grape in a series of wound inoculation test. B. ribis grew well on both potato sucrose agar and oatmeal agar. The best condition for vegetative growth on these two media was at $25{\sim}30^{\circ}C$ pH 4 and $10{\sim}15%$ sucrose content under light illumination. Rot development on fruit was first observed in the orchard at early August when sugar content in fruit reached 9.0%. Thereafter, number of rotted apples increased as sugar content increased. There was no correlation between the pH of juice of fruit and rot incidence. Infection on fruit began to occur as early as mid-June when young fruits were formed and infections were continued until harvest. When apple fruits were collected at 10-day intervals from the orchard beginning from early June and were wound-inoculated with B. ribis, rot lesion developed regardless of the stage of fruit growth. Incidence of white rot in the orchard was severe on Golden-delicious and Yukou, intermediate on Aoli, Fugi and Indo, and least on Jonathan and Red-delicious.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.1
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• pp.17-28
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• 2007

To understand the spring freezing injury symptoms during sensitive growth stage and yield loss of barley and wheat, field tests were done by using vinyl mulching and natural cold weather. The growth stage sensitive were booting, spikelet differentiation and flower organ development stages for both crops. However, barley and wheat differed in their growth responses, in that barley was less affected than wheat. For instance, barley recorded 28 percent dead ears, 10 percent dead plants and 18 percent ear degenerations while wheat recorded higher values of 59 percent, 44 percent and 44 percent, respectively. Although there were no recorded froze-resistant varieties in both barley and wheat, some showed tolerance as their yields were not affected by freezing stress. The 'Chalbori' cultivars of barley and 'Geurumil' and 'Chokwang' cultivars of wheat recorded steady yields. The yield components of barley and wheat that were greatly affected by freezing stress were the number of spike per square and the number of grain per spike. The major cause of yield loss in Suwon 259 and Kangbori was the number of spike per square but not the number of grain per spike. The study showed, however, that both the number of spike per square and the number of grain per spike were vulnerable to freezing and that which contribute much to yield loss of barley and wheat.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.113-119
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• 1989

In 1988 the tolerance of corn, sorghum, sorghum-sudangrass. and pearl millet hybrids to simazine and alachlor was tested in field during the growing season and pots during the summer and fall. In field and summer pot experiments(Exp.) the above mentioned four crops were tested at the ratios of simazine WP (50% ai, g/10a) ; alachlor EC (43.7% ai. ml/10a) of 130: 0, 100: 0, 70: 200, 0: 300 and 0: 400 and a sorghum hybrid was tested at 0, 50, 100, 200, 300, and 400ml/10a of alachlor and 70g/10a of simazine+ 200ml/10a of alachlor in fall pot Exp. In corn emergence rate, percent stand, plant height of seedlings, and dry matter(DM) yield were not affected by simazine and alachlor in all Exps. In sorghum and sorghum-sudangrass early growth and DM yield were not affected by simazine and alachlor in field Exp. In contrast, simazine reduced height and dry weight of seedlings slightly without any deterimental effects on emergence and survival rates. but alachlor reduced survival rate, plant height, and dry weight of seedlings significantly in summer pot Exp. In fall Exp. alachlor did not affect emergence rate of a sorghum hybrid, but survival rate, plant height, and dry weight of seedlings reduced with increased levels of alachlor when applied higher than 100ml/10a. In pearl millet simazine did not affect emergence rate, plant height, and DM yield in field, but reduced survival rate, plant height, and dry weight of seedlings in summer pot Exp. However, alachlor reduced DM yield significantly due to a lower percent stand even in the field. In summer pot Exp. although emeregence rate was slightly reduced, all seedlings were dead after emergence. Simazine did not control grasses such as Digitaria sanguinalis, Setaria viridis, Echinochloa crusgalli effectively, but controlled broadleaf weeds. Alachlor controlled all grasses, Porluraca oleracea, and Amaranthus mangostanus, but did not control Acalypha australis and Chenopodium album. A combination of simazine and alachlor controlled weeds more effectively than either simazine or alachlor alone.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.247-254
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• 2011

The agronomic characteristics, nutritional contents, and insect response of the potato clones transformed with a glufosinate ammonium resistance gene were evaluated. Among the 4 transgenic potato clones, the Bar 3 clone was selected as a promising one for commercialization. The Bar 3 clone showed similar tuber yield capacity but higher herbicide resistance as compared with the non-transgenic potato cv. Dejima. The herbicide resistance of the Bar 3 clone was more than 5 times higher when tested with the herbicide concentration recommended by the producer. The major agronomic characteristics of the Bar 3 clone were not different from those of the non-transgenic Dejima. The annual variation in yields and agronomic characteristics showed similar tendency for 2 years from the third to fourth generation after transformation. The tubers of the Bar 3 clone also showed low occurrence in common scab and physiological disorders such as cracking and secondary growth. But the reasons for such results are yet to be studied. Also, it was considered that the Bar 3 clone have a potential of reducing not only common scab occurrence but also soil erosion during potato cultivation in field. The nutritional contents (mineral compound, vitamin C and amino acid) and response to Spodoptera exigua of the transgenic potato clones were not significantly different.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.256-259
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• 2016

An obligate plant pathogenic fungus Puccinia zoysiae Dietel, the causal agent of rust disease, which is implicated in the damage of zoysiagrass (Zoysia spp.) in Korea. The fungus is one of the important pathogens, which attacks and colonizes susceptible zoysiagrass. Zoysia rust disease was observed in Jangseong, Hoengseong and Hapcheon in 2014 and 2015, Korea. The typical symptoms of the disease appeared first as small white spots on the leaf and stem of zoysiagrass plants, and turn brownish, dark brown spores revealed on the lesions. Uredospores were light brown in color, ellipsoid or spherical in shape and $22.0{\sim}25.0{\times}15.0{\sim}17.5{\mu}m$ in size. On the basis of the morphological characteristics, the fungus was identified as P. zoysiae. Pathogenicity of the fungus was proved by artificial inoculation on Z. japonica. The rust disease firstly appeared approx. early or middle June and then increased middle of October in Korea. In field, Z. matrella ecotypes were more resistant than Z. japonica ecotypes against the pathogen. Disease response against the pathogen was different among ecotypes of each species, respectively.

• The Korean Journal of Pesticide Science
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• v.15 no.2
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• pp.188-193
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• 2011

Resistance monitoring for tolyfluanid was conducted over 2 years from 2005 to 2006 with 218 isolates of Botrytis cinerea obtained from infected plants of ginseng. All isolates of B. cinerea were divided into 3 groups such as highly sensitive (HS) group, sensitive (S) group and less sensitive (LS) group to tolyfluanid. HS group was defined as one showing $EC_{50}$ value less than $2.0\;{\mu}g\;mL^{-1}$. $EC_{50}$ value of S group ranged between $2.0\;{\mu}g\;mL^{-1}$ and $18.0\;{\mu}g\;mL^{-1}$, while that of LS group was more than $18.0\;{\mu}g\;mL^{-1}$ Among B. cinerea isolates tested in this study, 174 isolates were included into S group, while 22 isolates were done into HS group. The other isolates belonged to LS group. Botrytis cinerea isolates showed the high correlation of $EC_{50}$ value of mycelial growth with that of spore germination to tolyfluanid. This results could be used for the determination of fungicide resistance and the establishment of strategy for fungicide resistance management.

• Research in Plant Disease
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• v.8 no.4
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• pp.239-244
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• 2002

Among 34 isolates of Colletotrichum spp., 20 from red pepper and 14 from apple, only one isolate 2001-45 was identified as the isolate resistant to sterol biosynthesis inhibiting fungicides. EC$_{50}$ values of the isolate 2001-45 were 153.5, 42.7, 34.0, and 17.1 $\mu\textrm{g}$/ml for myclobutanil, tebuconazole, hexaconazole and nuarimol, respectively, The resistance factor of the isolate 2001-45 against the other isolate 2001-44 to 4 above fungicides was ranged from 17 to 57. However, EC$_{50}$ value of the 2001-45 for prochloraz was 0.07 $\mu\textrm{g}$/ml, which was lower than those of the 2001-44 and the isolate JC24. For the fitness test of the 2001-45, mycelial growth, sporulation on PDA and pathogenicity on fruits were investigated. No difference in mycelial growth was found between 2001-45 and 2001-44, but great difference in sporulation. No symptom was developed by 2001-45 even by wound inoculation of pepper fruit. Therefore, this study indicated that the isolate 2001-45 was inferior to the other isolates in the fitness, although the isolate 2001-45 was highly resistant to sterol biosynthesis inhibiting fungicides.

• Korean Journal Plant Pathology
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• v.3 no.2
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• pp.93-99
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• 1987

Six rice cultivars showing various types of resistance or susceptibility to Pyricularia oryzae in the field were used to study the relationship between susceptibility and contents of carbohydrates, amino acids and phenolics at different plant maturities and leaf ages under controlled environmental conditions. No consistent differences between the susceptible cultivars and adult-plant-resistant cultivars were found in terms of the contents of individual carbohydrates, total amino acids and phenolics in rice leaves throughout the plant development. Only the adult-plant-resistant cultivar Dobong had lower contents of inositol throughout the plant development than the other cultivars. The amounts of sucrose, inositol, glucose, and fructose increased in all tested cultivars at eight leaf stage than those at five leaf stage, but slightly decreased at twelve leaf stage. In contrast, total amino acids and phenolics gradually decreased during plant development. With increasing age of rice leaves, the amounts of total soluble amino acids and phenolics gradually increased in healthy fifth leaf tissues, although there were no significant differences between the cultivars. In particular, a high level of phenolics existed in old fifth leaves of the cultivar Dobong. These results suggest that increased resistance to blast of matured rice plants and old leaves may be the two different phenomina derived from the physiological changes occurring during plant development and leaf senescence, probably functioning differently in P. oryzae development.

• Research in Plant Disease
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• v.16 no.1
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• pp.48-58
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• 2010

In Korea, EBI fungicides which are highly effective for control of pear scab and rust but of high risk for development of resistance have been frequently sprayed by majority of pear growers. To detect any possible resistance or reduced sensitivity in the field strains of scab fungus to five EBIs, difenoconazole, fluquinconazole, flusilazole, fenarimole and hexaconazole, sensitivity tests were conducted with fungal specimens collected in Ulsan and Naju where scab usually occurs and EBIs have been intensively sprayed for many years. As the strains for which $EC_{50}$ values of the EBIs were largely shifted from those of base-line were occasionally found, the resistant or less sensitive strains were supposed to be distributed. In the activity test for the EBIs by artificial inoculation, in which EBI-treated pear leaves on the potted seedlings were inoculated with fungal spores collected in the two regions, development of resistances to EBIs were confirmed. Since the fungal spores collected at 4 and 2 orchards in Naju and Ulsan, respectively, produced much higher disease incidence on the leaves treated with hexaconazole than those on the untreated control, those fungal specimens were determined as resistant to hexaconazole. Similar results were also obtained with two specimens from Ulsan for flusilazole.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.655-668
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• 2012

Foliar diseases are major constraints to profitable organic tomato production. Especially, powdery mildew, leaf mold and gray mold of tomato occur severely on organic cultured tomatoes in Korea. This study was conducted to develop organic tomato cultivation technology using environmental-friendly disease control methods (resistance cultivar planting, air circulation fan installation, oil-egg yolk mixtures, and microbial agents). When tomatoes were cultivated in plastic film house installed with air circulation fan, daily range of temperature was decreased by $2{\sim}7^{\circ}C$, average relative humidity was decreased by 1~5% compared to those in plastic house without air circulation fan. Consequently, incidence of tomato leaf mold and tomato gray mold was reduced by 55.0% and 24.4%, respectively. Control effect of microbial agents and oil-egg yolk mixtures against major tomato diseases was examined in plastic house. As a result, the control value of microbial agents against tomato gray mold and tomato leaf mold showed at the range of 49.0~55.9 %(gray mold) and 39.2~58.2%(leaf mold), respectively. The control value of oilegg yolk mixtures against tomato powdery mildew showed 97.6%. Fifteen tomato cultivars were evaluated for disease resistance against leaf mold and powdery mildew in organically cultivated tomato field. Among 15 tomato cultivars, seven cultivars including 'Super-top' were found to be high resistant to tomato leaf mold. Also 'Powerking', one of fifteen tomato cultivars, showed to be high resistant to tomato powdery mildew.