• The Korean Journal of Mycology
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• v.15 no.4
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• pp.238-246
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• 1987

Aspergillus niger van Tieghem was cultured by the method of synchronous and submerged culture. The sporulation occurred through the culture and its life cycle and differentiation were completed in the experiments. The effects of cAMP, theophylline and caffeine on the sporulation of A. niger were investigated. In the sporulation medium, the sporulation was stimulated by addition of cAMP and its optimum concentration was $10^{-4}M$. In the sporulation medium, the sporulation was stimulated by addition of theophylline and its optimum concentration was 10 mg/ml. In the sporulation medium, the sporulation was stimulated by addition of caffeine and its optimum concentration was 300 mg/ml. Theophylline added to the sporulation medium together with cAMP enhanced the promotion effect of cAMP on sporulation. Caffeine added to the sporulation medium together with cAMP enhanced the promotion effect of cAMP on sporulation. In the sporulation medium, the sporulation was stimulated by addition of neither AMP nor ATP. In the potassium acetate medium, cAMP, theophylline and caffeine stimulated the sporulation, respectively.

• The Korean Journal of Mycology
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• v.13 no.2
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• pp.65-74
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• 1985

Aspergillus niger IMI 41873 was cultured by the method of synchronous and submerged culture. Its sporulation occurred in the culture. Ribonucleic acids were extracted at each stage of life cycle. These RNAs were digested, separated and determined by P.E.I. cellulose TLC and HPLC methods. The levels of ribonucleic acids in sporulating mycelia were higher than those of conidiophore and phialide forming mycelia. Inosine 5-monophosphate and adenosine 5-monophosphate derivatives were found in HPLC separations. The levels of inosine 5-monophosphate and adenosine 5-monophosphate derivatives per ribonucleic acid were constant through differentiation. After the standard purine necleosides and boiling water extracts from A. bisporus, F. velutipes and L. edodes were added into the culture, their effects on sporulation were examined. Sporulation was greatly enhanced in each adding experiment.

• Journal of Plant Biology
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• v.6 no.1
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• pp.8-10
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• 1963

LA, Yong Joon (Coll. of Agr., Seoul National Univ.) Condial production of Cercospora beticola Sacc. on tomato juice agar. Kor. Jour. Bot. VI (1):8-10, 1963. Agar media containing various amount of tomato juice were tested to determine the degree of conidial production of Cercospora beticola. Non-sporulating culture on potato dextrose agar readily sporulated on agar media containing various amout of tomato juice 48 hours after transfer. Considerably small amount of sporulation occurred on agar media containing 10% of tomato juice. Sporulation increased considerably on media containing more than 20% of tomato juice; the higher the proportion of tomato juice in a medium, the greater the number of spores produced.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1975.07a
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• pp.109.1-109
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• 1975

The family Bacillaceae에 속하는 모든 미생물은 endospore를 형성하는 것이 그 특성이며 포자형성은 일련의 생화학적 반응은 물론 형태ㆍ구조적 변화를 수반하는 질서 정연한 원시적인 생물분화의 일종이다. 따라서 포자형성 발아에 관한 연구가 많이 이루어졌고 또 현재도 활발히 진행되고 있다. 산업미생물학도로서 흥미있는 점은 산업적으로 유용한 몇가지 생화학물질이 포자형성 과정과 특별히 관련되어 생산된다는 점이며 이중 몇 가지 효소와 항생물질 그리고 독소에 관하여 간략히 고찰해 보고자 한다.

• Korean journal of applied entomology
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• v.16 no.4 s.33
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• pp.211-215
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• 1977

The Sporulation of Didymella bryomiae were observed under diurnal cycles of light/darkness of near ultraviolet light (NUV) and artificial daylight (ADL) and continous darkness in eight isolates growing on PDA and V-8 juice agar. Light stimulated pycindial and perithecial formation of this fungus on potato dextrose agar and V-8 juice agar. Sprulation was poor in darkness, but some isolates were able to produce pycnidia and perithecia in the absence of light. Perithecial formation was much better under artificial daylight (ADL) on V-8 juice agar than those grown under near ultraviolet light (NUV). In general, cultures grown on V-8 juice agar sporulated better than cultures grown on PDA under three setsof light condition. Most of the pycnidiospores obtained from each isolates of this fungus grown on PDA were non-septate and microtype, but macrotype of non-septate and uniseptate pycnidiospores were produced on V-8 juice agar. Pycnidiospore produced on V-8 juice agar were similar to those produced on the radicle of naturally infected seeds. The appearance of perithecia were quite distinctive from pycnidia. The mature perithecia were darker than pycnidia and whitish spore masses formed on the ostiole of perithecia.

• Applied Biological Chemistry
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• v.10
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• pp.101-105
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• 1968

The effect of concentration of carbon and nitrogen sources on the sporulation and the life cycles of three strains of Zygosaccharomyces was investigated. The results are as follows: 1) The good sporulation of Zygosaccharomyces bisporus, delbruekii, and Z. steineri was obtained on solid medium containing 0 to 0.001% of nitrogen and 10 to 20% of glucose. The high content of nitrogen was detrimental to sporulation and asci were formed under 0.01% of nitrogen. 2) It is widely accepted that the life cycle of Zygosaccharomyces proceeds in the following way: Ascospore...Vegetative cells...Conjugation of vegetative cells...Sporulation...Ascopores But zygotes of Z. bisporus proceeded to vegetative cells when transfered to the suitable medium for vegetative reproduction, and then formed asci.

• Korean journal of applied entomology
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• v.20 no.1 s.46
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• pp.21-24
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• 1981

This study was conducted to obtain a supply of conidia sufficient for screening mungbean mutant lines for a source of resistance to Cercospora leaf spot caused by Cercospora canescens Ellis and Martin. Abundant sporulation occurred in cultures on mungbean leaf decoction oatmeal agar(MOA) exposed to about 2,500 Lux of fluorescent light. but it did not occur in continuous darkness. The conditions that produced maximum number of conidia was not coincided with those for vegetative growth and pigmentation in culture medium. Removal of aerial mycelium in culture by brushing with sterile water so enhanced the conidial production that oatmeal agar medium(OA) could be useful for production of abundant conidia by the treatment.

• Research in Plant Disease
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• v.29 no.3
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• pp.251-257
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• 2023

In order to examine the pathogenicity of Alternaria dauci, the causal agent of carrot leaf blight, it is necessary to standardize sporulation conditions to ensure the optimal quantity and quality of spore inoculum. Therefore, in this study, the effects of the growth medium, aeration treatment, and UV treatment with 12-hr photoperiod on the sporulation of A. dauci KACC42997 were investigated. A. dauci KACC42997 was pre-cultured for 7 days in a potato dextrose agar medium at 25℃ in the dark condition. When the pre-culture, after removing aerial mycelia, was re-incubated for 5 days, with simultaneous aeration treatment and 12-hr cycle UV treatment at 20℃, the largest number of spores was produced. One hundred seventy isolates of A. dauci were isolated from major carrot growing regions such as Pyeongchang, Gumi and Jeju and tested for sporulation under the conditions established in this study. Except for 20 strains, all strains produced spores. Statistically significant differences in the extent of sporulation were found among local populations of A. dauci isolates, but no difference was observed in their pathogenicity on carrots.

• Korean journal of applied entomology
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• v.19 no.3 s.44
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• pp.169-174
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• 1980

Regardless of the kind of the apple orchards whether received various fungicides application or not Rovral exhibited the most efficient inhibitory effect on the sporulation of Alternaria mali with the spotted leaf disks at the recommended dose $0.5{\mu}g/ml$ as well as the lower or the higher doses. When applied at the recommended doses $0.7{\mu}g,\;0.75{\mu}g,\;and\;0.7{\mu}g/ml$ of Antracol, Dithane M-45, and Dikar, respectively, these fungicides showed complete inhibitory effects on the sporulation. At the lower levels of application, they allowed the sporulation of 10,000 to 25,000 conidia/ml. Polyoxin and Difolatan, when applied on the leaf disks from the orchard that had received the fungicides application, exhibited low effects allowing the sporulation of around 20,000 conidia/ml. On the leaf disks from the orchard that had received no fungicide application, however, both fungicides showed relatively high inhibitory effects of the sporulation. The inhibitory effects of Captan, Dakonil, and Spat on the sporulation were lower than those of the aforementioned fungicides. Bayleton was the least effective among the fungicides used in the present investigation. Benlate showed promoting effect on the sporulation of the apple leaf spot fungus rather tan inhibiting.

• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.219-255
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• 1996

사과 겹무늬썩음병의 주 전염원은 가지 병반에서 형성되는 자낭포자 및 병포자이므로, 가지에서의 포자형성 또는 포자의 분산을 화학적 또는 물리적 방법으로 저해할 수만 있다면 효과적으로 병을 방제할 수 있을 것으로 생각했다. 전염원을 줄이기 위한 첫 번째 시도로 1992년 이른봄 가지에 형성된 사마귀를 호미로 제거한 결과, 병포자의 분산과 과실발병 억제효과에 있어서 무처리와 유의적인 차이가 인정되지 않았다. 두 번째 시도로 포자의 분산을 막기 위해 1993년 이른봄에 겹무늬썩음병에 걸린 가지를 비닐필름으로 감고 5월부터 9월까지 20일 간격으로 살균제를 살포한 결과, 통계적으로 유의성이 인정되는 병 방제효과가 있었고, 나무의 생육에 있어서도 콜크층이 이상 비대하는 것 이외에 별다른 이상이 없었다. 이 개념을 더욱 발전시켜 1994년 이른봄에는 병든 가지를 수용성 고분자 물질인 polyvinyl alcohol로 도포하고 수배 분산 포자의 수를 조사한 결과, 포자의 분산량이 크게 줄어져 실용화의 가능성이 시사되었다.