Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Factors Affecting Spore Formation of Carrot Leaf Blight Caused by Alternaria dauci In Vitro

배지에서 당근검은잎마름병균의 포자 형성에 영향을 미치는 요인

  • Sena Yoon (Department of Plant Medicine, Chungbuk National University) ;
  • Jiyoung Min (Department of Plant Medicine, Chungbuk National University) ;
  • Heung Tae Kim (Department of Plant Medicine, Chungbuk National University)
  • 윤세나 (충북대학교 농업생명환경대학 식물의학과) ;
  • 민지영 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2023.08.11
  • Accepted : 2023.08.28
  • Published : 2023.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In order to examine the pathogenicity of Alternaria dauci, the causal agent of carrot leaf blight, it is necessary to standardize sporulation conditions to ensure the optimal quantity and quality of spore inoculum. Therefore, in this study, the effects of the growth medium, aeration treatment, and UV treatment with 12-hr photoperiod on the sporulation of A. dauci KACC42997 were investigated. A. dauci KACC42997 was pre-cultured for 7 days in a potato dextrose agar medium at 25℃ in the dark condition. When the pre-culture, after removing aerial mycelia, was re-incubated for 5 days, with simultaneous aeration treatment and 12-hr cycle UV treatment at 20℃, the largest number of spores was produced. One hundred seventy isolates of A. dauci were isolated from major carrot growing regions such as Pyeongchang, Gumi and Jeju and tested for sporulation under the conditions established in this study. Except for 20 strains, all strains produced spores. Statistically significant differences in the extent of sporulation were found among local populations of A. dauci isolates, but no difference was observed in their pathogenicity on carrots.

당근 검은잎마름병을 일으키는 Alternaria dauci의 병원성 검정을 위해서는 양과 질이 우수한 포자를 형성할 수 있는 조건의 표준화가 필요하다. 따라서 본 실험에서는 배지의 종류, 통기 처리, UV 처리 등이 A. dauci KACC42997의 포자 형성에 미치는 효과를 조사하였다. A. dauci KACC42997은 25℃, 암 상태의 PDA 배지에서 7일간 전배양하였다. 배지 상에 나타난 병원균의 공중 균사를 제거하고, 20℃에서 통기 처리와 12시간 주기의 UV 처리를 동시에 실시하며 5일간 배양하였을 때, 가장 많은 포자를 형성하였다. 확립 포자 형성 조건에서 평창, 구미, 제주 등 당근의 주요 재배지역에서 분리한 170개의 다양한 A. dauci 균주가 포자를 형성하는지의 여부를 확인한 결과, 20개 균주가 포자를 형성하지 못하였고, 88.2%의 균주는 포자를 형성하였다. 또한 지역 균주 집단 간의 포자 형성량에는 차이가 있었지만, 당근에 대한 병원성에는 차이가 없었다.

Keywords

Acknowledgement

This work was supported by a funding for the academic research program of Chungbuk National University in 2022.

References

  1. Bounds, R. S., Podolsky, R. H. and Hausbeck, M. K. 2007. Integrating disease thresholds with TOM-CAST for carrot foliar blight management. Plant Dis. 91: 798-804. https://doi.org/10.1094/PDIS-91-7-0798
  2. Campbell, M. A., Medd, R. W. and Brown, J. B. 2003. Optimizing conditions for growth and sporulation of Pyrenophora semeniperda. Plant Pathol. 52: 448-454. https://doi.org/10.1046/j.1365-3059.2003.00872.x
  3. Douglas, D. R. 1972. The effect of light and temperature on the sporulation of different isolates of Alternaria solani. Can. J. Bot. 50: 629-634. https://doi.org/10.1139/b72-077
  4. Dugdale, L. J., Mortimer, A. M., Isaac, S. and Collin, H. A. 2000. Disease response of carrot and carrot somaclones to Alternaria dauci. Plant Pathol. 49: 57-67. https://doi.org/10.1046/j.1365-3059.2000.00389.x
  5. Farrar, J. J., Pryor, B. M. and Davis, R. M. 2004. Alternaria diseases of carrot. Plant Dis. 88: 776-784. https://doi.org/10.1094/PDIS.2004.88.8.776
  6. Fourtouni, A., Manetas, Y. and Christias, C. 1998. Effect of UV-B radiation on growth, pigmentation, and spore production in the phytopathogenic fungus Alternaria solani. Can. J. Bot. 76: 2093-2099.
  7. Grogan, R. G., Kimble, K. A. and Misaghi, I. 1975. A stem canker disease of tomato caused by Alternaria alternata f. sp. lycopersici. Phytopathology 65: 880-886. https://doi.org/10.1094/Phyto-65-880
  8. Houston, B. R. and Oswald, J. W. 1946. The effect of light and temperature on conidium production by Helminthosporium gramineum in culture. Phytopathology 36: 1049-1055.
  9. Jimenez, M. F. and Miller, R C. 1966. Effect of light on the sporulation of Alternaria tenuis. Phytopathology 56: 883.
  10. Kwon, M. Ryu, K. Y., Kim, J. S. and Shin, G. Y. 2007. Occurrence pattern of pests in carrot fields and effect of plant debris removal after harvest at hightland area. Korean J. Hortic. Sci. Techonol. 25: 316-321. (In Korean)
  11. Langenberg, W. J., Sutton, J. C. and Gillespie, T. J. 1977. Relation of weather variables and periodicities of airborne spores of Alternaria dauci. Phytopathology 67: 879-883. https://doi.org/10.1094/Phyto-67-879
  12. Latham, A. J. 1974. Effect of moisture on conidiophore morphology of Cristulariella pyramidalis. Phytopathology 64: 1255-1257. https://doi.org/10.1094/Phyto-64-1255
  13. Li, C., Sachs, M. S. and Schmidhauser, T. J. 1997. Developmental and photoregulation of three Neurospora crassa caratenogenic genes during conidiation induced by dessication. Fungal Genet. Biol. 21: 101-108. https://doi.org/10.1006/fgbi.1996.0948
  14. Lukens, R. J. and Horsfall, J. G. 1973. Processes of sporulation in Alternaria solani and their response to metabolic inhibitors. Phytopathology 63: 176-182. https://doi.org/10.1094/Phyto-63-176
  15. Miller, P. M. 1955. V-8 juice agar as a general purpose medium for fungi and bacteria. Phytopathology 45: 461-462.
  16. Park, K. H., Yun, H. J., Ryu, K. Y., Yun, J. C., Kim, S. R., Kim, W. I. et al. 2011. Influence of environmental factors on conidial germination of Alternaria dauci. Res. Plant Dis. 17: 381-385. (In Korean) https://doi.org/10.5423/RPD.2011.17.3.381
  17. Pruss, S., Fetzner, R., Seither, K., Herr, A., Pfeiffer, E., Metzler, M. et al. 2014. Role of the Alternaria alternata blue-light receptor LreA (white collar 1) in spore formation and secondary metabolism. Appl. Environ. Microbiol. 80: 2582-2591. https://doi.org/10.1128/AEM.00327-14
  18. Pryor, B. M., Strandberg, J. O., Davis, R. M., Nunez, J. J. and Gilbertson, R. L. 2002. Survival and persistence of Alternaria dauci in carrot cropping systems. Plant Dis. 86: 1115-1122. https://doi.org/10.1094/PDIS.2002.86.10.1115
  19. Rodrigues, T. T. M. S., Maffia, L. A., Dhingra, O. D. and Mizubuti, E. S. G. 2010. In vitro production of conidia of Alternaria solani. Trop. Plant Pathol. 35: 203-212. https://doi.org/10.1590/S1982-56762010000400001
  20. Schouten, H. J., van Tongeren, C. A. M. and van den Bulk, R. W. 2002. Fitness effects of Alternaria dauci on wild carrot in The Netherlands. Environ. Biosafety Res. 1: 39-47. https://doi.org/10.1051/ebr:2002004
  21. Shahin, E. A. and Shepard, J. F. 1979. An efficient technique for inducing profuse sporulation of Alternaria species. Phytopathology 69: 618-620. https://doi.org/10.1094/Phyto-69-618
  22. Soteros, J. J. 1979. Pathogenicity and control of Alternaria radicina and A. dauci from imported carrot seed in New Zealand. N. Z. J. Agric. Res. 22: 191-196. https://doi.org/10.1080/00288233.1979.10420860
  23. Strandberg, J. O. 1983. Infection and colonization of inflorescences and mericarps of carrot by Alternaria dauci. Plant Dis. 67: 1351-1353. https://doi.org/10.1094/PD-67-1351
  24. Vargas, J. M. and Wilcoxson, R. D. 1969. Some effects of temperature and radiation on sporulation by Helminthosporium dictyoides on agar media. Phytopathology 59: 1706-1712.