• Journal of fish pathology
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• v.12 no.1
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• pp.32-41
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• 1999

Ozonization for rearing seawater in land-based culture system has recently been utilized for disinfection of pathogenic microorganisms and improvement of water quality. This study was conducted to examine the effects of total residual oxidants (TRO) in ozone-treated seawater on survival, blood parameters, osmolality and oxygen consumption, and gill tissue of the flounder. Paralichthys olivaceus. Experiments were carried out with the starved flounder of 12~19 cm in total length at $20^{\circ}C$. The 48-hr and 96-hr $LC_{50}$ for the flounder amounts to 26.4 ppb and 22.3 ppb, respectively. With increase of TRO concentration from 24 to 39 ppb, the values of hematocrit, hemoglobin concentration, red blood cell count and osmolality of the flounder with respect to exposure time were significantly elevated, however, the oxygen consumption rates decreased. In the case of the fish exposed to 13 ppb for 96 hrs, blood glucose increased with an elapse of exposure time, while survival rate was 100%. Death apparently resulted by massive destruction of gill lamellar epithelium, severe osmotic imbalance and the lack of oxygen uptake. The results of this experiment indicated that to protect aquaculture organisms, the ozone-treated seawater should not contain any residual oxidants, and that relatively long-term exposure to TRO of low concentration can impact on survival and physiological conditions of the flounder.

• Korean Journal of Food Science and Technology
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• v.30 no.4
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• pp.775-780
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• 1998

Gamma irradiation at 5 kGy was applied to beefs for evaluation of their possible genotoxicity and acute oral toxicity. The genotoxicity of 5 kGy irradiated beef was evaluated by Salmonella typhimurium reversion assay and in vivo micronucleus assay using mouse bone marrow cells. The results were negative in the bacterial reversion assay with S. typhimurium TA98, TA100, TA1535, TA1537. Clastogenic effects were not shown in vivo mouse micronucleus assay at 5 kGy dose tested. In an acute toxicity test, 5 kGy-irradiated beef was administrated orally at a dose level of 313 to 5,000 mg/kg, and then number of deaths, clinical signs, body weights, and pathological examinations were examined daily for 14 days post-administration. The results indicate that 5 kGy irradiated beef did not show any toxic effect on mice and oral $LD_{50}$ value was over 5,000 mg/kg on ICR mice.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.544-544
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• 2015

국내에는 17,500여개의 농업용 저수지가 전국적으로 분포하고 있으며, 이들 저수지는 인공호소이다. 농업용 저수지의 총 유효저수량은 2,838백만$m^3$에 달하고 있으며, 전체 수리답의 약 76%에 해당하는 593천ha의 농경지에 물을 관개하고 있다(2013농업생산기반통계연보). 최근 농어촌지역의 도농복합형태 개발 및 관광지, 유원지화로 농업용 저수지 유역의 오염원이 지속적으로 증가하는 추세에 있으며, 이로 인해 농업용수 수질이 지속적으로 악화되고 있다. 특히, 늦봄에서 초가을까지 외기온도 상승 및 저수율 저하와 함께 부영양화 증가, 녹조 대발생 등으로 수질오염도가 급상승하고 있으며, 이로 인해 호 내 물고기 폐사, 악취발생 등으로 민원이 다수 발생하고 있다. 본 연구에서는 농업용 저수지의 물리적 특성을 분석하여 농업용 저수지 수질개선을 위한 기초자료로 활용하고자 하였다. 농업용 저수지 17,489개소를 수혜면적별로 분류해 보면 5ha이하가 7,808개소(44.7%), 5~10ha이하 4,277개소(24.5%), 10~20ha 2,772개소(15.9%), 20~30ha 797개소(4.6%), 30~40ha 385개소(2.2%), 40~50ha 238개소(1.4%), 50ha이상 1,201개소(6.9%)로 구성되어 있어 50ha이하가 전체의 93.1%에 이르고 있다. 유효저수량별 분포현황은 10천$m^3$이하가 7,996개소(45.7%), 10~50천$m^3$ 6,420개소(36.7%), 50~100천$m^3$ 1,003개소(5.7%), 100~300천$m^3$ 868개소(5.0%)로 전체 시설 중 50천$m^3$이하가 전체 저수지의 82.4%로 대부분이 소규모 저수지임을 알 수 있다. 농업용 저수지를 유효수심별로 구분해보면 1m이하가 전체의 26.6%, 3m이하가 전체의 77.1%, 5m이하가 전체의 91.1%로 국내 대부분의 저수지들은 수심이 5m 이하의 저류지 형태의 저수지임을 알 수 있다. 저수지를 유역배율(유역면적/만수면적)에 따라 구분해보면 유역배율 3이하가 전체의 1.2%이고, 5이상이 전체의 97.4%를 차지하고 있다. 일반적으로 유역배율이 3이상이면 부영양화에 취약한 호소로 분류되고 있다. 국내 농업용 저수지는 대부분이 수심이 낮고, 유효저수량이 소규모이며, 유역배율이 3이상인 호소가 대부분으로 태생적으로 수질오염 및 부영양화에 취약한 구조로 되어 있음을 알 수 있다. 농업용 저수지 수질개선을 위한 종합계획 수립시에는 유입수 및 호내 대책과 함께 호소의 물리적 조건을 개선시키는 방안에 대해서도 검토가 요구된다. 특히 저수지 신규 설치시에는 수량관리 뿐만 아니라 수질도 함께 고려된 물리적 인자에 대한 설계가 이루어져야 할 것으로 판단된다.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.431-431
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• 2016

J 호소는 1942년도에 충청남도 아산시 선장면에 설치된 농업용 저수지로서, 현재 COD, TN, TP 모두 등급외의 수질상태를 보이고 있다. J 호소 상류 유역은 축산농가가 다수 위치하고 있으며, 설치된 지 70년이 지난 노후화된 저수지로 고농도의 오염물질 유입뿐만 아니라 호소 바닥의 오염 퇴적물에 의한 내부 부하가 중요한 수질오염원이 되고 있다. 호소의 수질은 현재 COD 10.6~16.5 mg/L, Chl-a $75mg/m^3$ 이상으로 수질오염도가 매우 높으며, 특히 늦봄에서 초가을까지 외기온도 상승 및 저수율 저하와 함께 부영양화 증가, 녹조 대발생 등으로 호 내 물고기 대량 폐사 및 악취발생 등으로 민원이 다수 발생하고 있다. 이러한 현상의 직접적인 원인은 수중의 DO 농도 결핍이며, 따라서 수중의 DO 농도를 일정수준 이상으로 유지시켜 주는 것은 호소 수질관리를 위해 매우 필요하다고 판단된다. 본 연구에서는 공기 중의 산소를 호소 수체에 포기시켜 주는 마이크로버블 발생장치를 J 저수지에 설치하여 수체의 DO 농도 변화 등을 분석하였고, 본 연구결과는 농업용 호소의 수질개선을 위한 기술개발 및 계획수립의 기초자료로 활용하고자 하였다. 마이크로버블 포기장치는 수심이 약 4m 되는 저수지 제방 근처에 100m 간격으로 총 3지점에 설치하였다. 버블 발생기는 기액 2상류 선회형 마이크로 버블 발생장치로 3지점에 각 1set(1set에 3기로 구성)씩 구성하여 저수지 바닥 층에서 상부로 1m 떨어진 지점에 고정식으로 설치하였다. 총 공기흡입량은 380 LPM이며, 사용동력은 12.2kW를 사용하였다. 마이크로 버블 포기장치 설치 후 호 내 DO 농도 변화를 평가하기 위하여 호소 전체에 18개 지점을 선정하여 수심 50cm 간격으로 DO 농도를 측정하였다. 가동 전에는 DO 평균농도가 표층에서는 약 7.7mg/L로 나타났고, 수심에 따라 거의 수직적으로 감소하여 바닥층에서는 약 0.2mg/L로 거의 무산소 상태를 보이고 있었다. 마이크로 버블 가동 2주 후에는 수심 3m까지의 모든 수층에서 DO 농도가 약 6.0mg/L 이상을 보였고, 바닥층에서는 DO 약 3.4mg/의 농도를 나타내었다. 가동 3주 후에도 2주 후와 비슷한 수치를 보이고 있었으나 가동 4주가 지나면서부터는 호소 바닥층(수심 3.5m)에서도 DO 농도가 7.0mg/L 이상의 높은 농도를 유지하는 것으로 조사되었다. 호소 저층에서 호기성 상태의 지속적 유지는 퇴적 오염물질이 수층으로 용출되는 것을 예방할 수 있으므로 마이크로버블을 잘 활용하면 호소의 악취제거 및 수질개선에 많은 도움이 될 것으로 판단된다.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.6
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• pp.1366-1372
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• 2014

This study was conducted to select the media for the formalin killed vaccine (FKC) production of Vibrio harveyi and its application for olive flounder, Paralichthys olivaceus. For this, we have investigated the immune effects of Vibrio harveyi FKC vaccines grown in 3 different media i.e. Tryptic Soy Broth (TSB), TSB containing 2-2'-dipyridyl (TSB-D), Brain Heart Infusion Broth (BHIB) on the production of agglutinating antibody and protection against Vibrio harveyi in olive flounder. Additionally, a dual vaccine was prepared by combining Streptococcus parauberis vaccine to V. harveyi vaccine and its efficacy was also analyzed with the determination of optimal administration dosage. Consequently, olive flounder immunized with FKC grown in TSB-D showed the same protection with the vaccine grown in BHIB and the optimal dose of the vaccine was 10mg/kg of body weight. Indeed the dual vaccine showed higher agglutination titer and protection than control fish. The optimal dose for dual vaccine was 10mg/kg body weight for each of two vaccines.

• Journal of Korean Society of Forest Science
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• v.112 no.1
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• pp.117-125
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• 2023

Outbreaks of Ramulus mikado (Insecta: Phasmatodea: Phasmatidae) in the hilly areas of Mt. Bongsan, Mt. Cheonggye, and elsewhere in Seoul and Gyeonggi occurred from 2020 to 2021, causing serious defoliation. We evaluated the insecticidal effects of six eco-friendly organic materials and the insect-pathogenic fungus Metarhizium anisopliae against R. mikado. The fungus was isolated from naturally dead bodies of R. mikado in forest ecosystems. The results revealed that three eco-friendly organic materials containing azadirachtin or geraniol as active ingredients showed high mortality in the range of 85.2%-100%, which were rates similar to that of the chemical insecticide fenitrothion emulsifiable concentrate. All R. mikado adults that were sprayed with a conidial suspension of M. anisopliae at different concentrations were killed within a few days. In conclusion, three eco-friendly organic materials and M. anisopliae could be good alternatives to chemical insecticides.

• Journal of Aquaculture
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• v.20 no.4
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• pp.270-277
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• 2007

White spot syndrome virus (WSSV) which is the most serious threat to cultured shrimp around the world has given enormous economic damages to shrimp culture industry every year since it was found from the shrimp ponds in the west coast of the South Korea in 1993. WSSV has strong infectivity as well as virulence and it can be rapidly transmitted among shrimps in ponds by cannibalism of infected ones. Polyculture of shrimps with carnivorous fish has been applied in commercial shrimp farms to suppress or delay the viral outbreak because the fish may selectively eat the moribund shrimps infected by virus. To determine the selective predatory effect of a carnivorous fish, river puffer Takifugu obscurus on white shrimp Litopenaeus vannamei, polyculture trials in laboratory scale of WSSV-infected and non-infected shrimps with river puffer were conducted in concrete round tanks of $28.26\;m^2$ in surface area as followings: 1) juvenile shrimps (B. W. 0.62 g) with 5 months old puffer (B. W. 11.60 g) cultured for 8 days, and 2) sub-adult shrimps (B. W. 6.84 g) with 16 months old puffer (B. W. 85.82 g) cultured for 5 days in order to know the effects according to size difference of cultured animals. In polyculture of juvenile shrimp with 5 months old puffer, survival rates of infected and non-infected shrimps were 46.0% and 89.1% respectively and in that of sub-adult shrimp with 16 months old puffer those were4% and 48% respectively. The results showed that puffer tends to selectively prey on virus infected shrimps among infected and non-infected ones in a limited space with although there is difference in predatory rate with age and density of animals. Regardless of different densities and ages of animals as well as health condition of shrimps, however, there were low differences in daily biomass of shrimp consumed per kg body weight of puffer. This finding suggests that puffer preys on healthy shrimps when moribund shrimps were not sufficient. Therefore, farmers should consider the total biomass of puffer as well as density and stocking time when they stock puffer into shrimp ponds for polyculture.

• Journal of Aquaculture
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• v.18 no.2
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• pp.107-114
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• 2005

Clownfish are important and very popular fish in the ornamental aquarium industry. Demand for the fish is increasing dramatically. The present study was conducted to verify methods of broodstock management, patterns of spawning, rates of egg hatching and estimates of larval growth fur the saddleback clownfish, Amphiprion polymnus. Spawning occurred 8 times between August 2002 to June 2004 with 2 females and 1 male participating. Fertilized eggs were separated by an adhesive matrix and were oval in shape. The eggs were $2.46{\pm}0.13mm$ in size as measured along the longest axis. The percentage of fertilized eggs was 96.7%. Hatching was observed seven days post-spawning and hatching rate was 85.5%. The sizes of the newly-hatched larvae were $4.58{\pm}0.21mm$ TL (total length). Larvae had an open mouth and anus, and an oval yolk sac. At the 1 st day after hatching, the sizes of the larvae were $4.90{\pm}0.35mm$ TL. The larvae began to eat rotifers after complete yolk absorption. On the 5th day post-hatch, larvae were $5.88{\pm}0.31mm$ TL with complete fins and the survival rate was 48.6%. At 8 days after hatching, a band began to appear on head and back of the larvae indicating the beginning of metamorphosis. Metamorphosis was completed at an average TL of $15.00{\pm}2.12mm$ on the 23rd day after hatching. By the 45th day after hatching, juveniles averaged $22.76{\pm}3.22mm$ TL and survival rate was 28.4%.

• Journal of Animal Science and Technology
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• v.45 no.2
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• pp.229-240
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• 2003

An in vitro test and a broiler feeding trial were conducted to test the effect of hydrothermal treatment of wheat bran on phytate-P degradation and it’s feeding effect on performance of broilers. Hydrothermal treatment of wheat bran was carried out at 55$^{\circ}C$ with pH 5.5 buffer solution. Phytate-P content of wheat bran decreased quadrically as the wheat bran: buffer solution ratio increased from 1:0.5 to 1:5. Phytate-P degradation was not significantly affected by incubation times above 10 min., drying temperature (55$^{\circ}C$, 65$^{\circ}C$ and 75$^{\circ}C$) or pH of the buffer solution (5.5 and 7.0). A feeding trial was conducted with 240 sex separated d-old broiler chickens (Ross$^{\circledR}$). Broilers were randomly housed to 24 cages of 10 birds each. Six cages (3 of each sex) were assigned to 4 treatments: Control-normal level of non-phytate-P (NPP); LP-low NPP treatment which had 0.1% lower NPP than Control; LPWB-LP with wheat bran which provided 475 IU of plant phytase per kg diet; LPHWB-LP with hydrothermally treated wheat bran. Results of the feeding trial showed that broilers in the LP treatment gained significantly less than other treatments in starter period (1-21d) but only male broilers for growing LP gained significantly less than Control in the grower (22-35d) and overall period. There were no significant differences in weight gain among the birds of LPWB, LPHWB and Control. Feed intake during the overall period was not significantly different between LPWB and Control but that of LP was lower than LPHWB and that of LPHWB was lower than Control. Feed/gain ratio was significantly lower in LPHWB and LP than in Control and LPWP. Mortality was highest in LPHWB. Availability of crude fat, crude ash and Ca was significantly lower in LP than other treatments. Availability of P and Zn was higher in LPWB and LPHWB than in Control and LP. Availability of P, Mg and Zn was highest in LPHWP treatment. Excretion of P was significantly lower in low NPP treatments than in Control. Serum Ca level was highest whereas serum P level was lowest in LP. Tibial crude ash content was higher in wheat bran treatments, but lower in LP than Control. However, tibial Ca content was higher in Control and LP than wheat bran treatments. Tibial P content of LP and LPWB was lower than Control. However, tibial content of Fe was highest in LP. It was concluded that wheat bran, a source of plant phytase, could be used in low NPP broiler diets to prevent the depression of performance. Reduction of P excretion can be achieved concomitantly. Hydrothermal treatment of wheat bran was effective in improving utilizability of some minerals but was not effective in improving performance of broilers.

• Journal of Aquaculture
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• v.19 no.4
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• pp.247-253
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• 2006

This study was conducted to investigate the effects of dietary supplementation of ${\beta}-1,3$ glucan on growth and immune responses in juvenile olive flounder, Paralichthys olivaceus fed the white fish meal based diets for 6 weeks. Five experimental diets supplemented with ${\beta}-1,3$ glucan at 0, 0.01, 0.025, 0.05, 0.1 % (Control, $G_{0.01},\;G_{0.025},\;G_{0.05}\;and\;G_{0.1}$, respectively) of diet on a dry-matter basis. Five experimental diets were formulated to be isonitrogenous and isocaloric to contain 50.0% crude protein and 16.7 kJ available energy $g^{-1}$. Fish averaging $3.2{\pm}0.1\;g\;(mean{\pm}SD)$ were randomly distributed in each aquarium as triplicate groups of 15 fish. Weight gain (WG, %), specific growth rate (SGR, %), and feed efficiency (FE, %) of fish fed $G_{0.1}$ diet were found significantly higher than those of fish fed Control, $G_{0.01},\;G_{0.025}\;and\;G_{0.05}$ diets (P<0.05). However, there was no significant difference among the fish fed control, $G_{0.01},\;G_{0.025}$. Chemiluminescent responses (CL) of fish fed $G_{0.1}$ diet were significantly higher than those of fish fed the other diets. Serum lysozyme activities of fish fed $G_{0.05}$ and $G_{0.1}$ diets were higher than those of fish fed control, $G_{0.025}$ and $G_{0.05}$ diets. Fish fed $G_{0.1}$ diet showed a significantly lower cumulative mortality than did fish fed control diet throughout the challenge test (P<0.05). These results suggested that based on growth rate, feed efficiency, non-specific immunity and protection against microbial infections the optimum dietary ${\beta}-1,3$ gulcan could be greater than 0.05% but less than 1.0% in juvenile olive flounder, Paralichthys oilvaceus.