• Title/Summary/Keyword: 페놀성화합물

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Development of Extracts of Lycii folium Having High Antioxidant Activity (항산화 활성이 높은 구기엽 추출물 제조)

  • Kim, Tae-Su;Park, Won-Jeong;Ko, Sang-Beom;Kang, Myung-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.10
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    • pp.1318-1322
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    • 2008
  • The aim of this study was to analyze the antioxidant properties of Lycii folium extracts prepared from different solvents. Lycii folium were extracted with water, 80% ethanol (80% EtOH), 80% methanol (80% MeOH) and 100% methanol (100% MeOH) in water bath at $40^{\circ}C$. The antioxidant activity of the extracts was evaluated using DPPH, hydroxyl and hydrogen radical scavenging activities, and SOD-liked activity. Total phenolic acid contents were 1.085 mg/mL in 100% MeOH, 1.382 mg/mL in 80% EtOH, 1.420 mg/mL in 80% MeOH and 1.084 mg/mL in water. DPPH radical scavenging activity of the extracts were 65.60% in 80% EtOH, 56.80% in 80% MeOH, 83.85% in 100% MeOH and 54.65% in water. Hydroxyl radical scavenging activities were 66.65% in 100% MeOH, 73.13% in 80% ethanol, 73.58% in 80% MeOH and 70.73% in water. Hydrogen radical scavenging activity of the extracts prepared from Lycii folium were 11.70% in 100% MeOH, 33.73% in 80% EtOH, 35.40% in 80% M eOH and 23.86% in water. SOD-liked activity of the extracts prepared from Lycii folium was 71.58% in 100% MeOH, 74.29% in 80% EtOH, 88.46% in 80% MeOH and 67.47% in water. Our result showed that Lycii folium extracts prepared from 80% methanol were found to be promising biomaterials with antioxidant effects.

Quality Properties of Yogurt Added with Hot Water Concentrates from Allium hookeri Root (삼채 뿌리 열수 농축물을 첨가한 발효유의 품질특성)

  • Jun, Hyun-Il;Park, Seon-Yeong;Jeong, Do-Yeon;Song, Geun-Seoup;Kim, Young-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.9
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    • pp.1415-1422
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    • 2014
  • Hot water extraction concentrate was prepared from Alliun hookeri root (AHR) to evaluate its applicability to yogurt. The highest antioxidant activity of hot water concentrates was obtained under extraction conditions of 4 hr at $95^{\circ}C$. Antioxidant activities measured by DPPH radical assay, ABTS radical cation assay, reducing power, and cheating activity were highly correlated with total phenolic (89.51 mg/g) and total flavonoid (52.71 mg/g) contents, with R values of 0.94 and 0.96, respectively. Yogurt was fermented with a commercial lactic acid bacteria mixed strain (Yo-mix$^{TM}$ 305) for 10 hr at $42^{\circ}C$ after addition of 0~10% (w/w) hot water concentrates from AHR to yogurt base. As fermentation proceeded, pH and $^{\circ}Brix$ of yogurt decreased from 6.57~6.60 to 4.34~4.51 and from 8.10~8.90% to 4.60~5.25%, respectively, whereas titrate acidity, viscosity, and viable cell numbers increased from 0.22~0.23% to 1.01~1.10%, from $0mPa{\cdot}s$ to $202.55{\sim}290.50mPa{\cdot}s$, and from 6.40~6.80 log CFU/mL to 8.60~9.20 log CFU/mL, respectively. There was no significant difference in any sensory attribute between the control and 2.5% addition group, suggesting that 2.5% hot water concentrate from AHR could be used to manufacture yogurt.

Effects of Extraction Methods of Medicinal Plants on Human Growth of Neuroblastoma SK-N-SH Cells (추출방법에 따른 한약재의 인체신경모세포 SK-N-SH 보호 효과)

  • Kwon, Jung-Min;Moon, Yeon-Gyu;Kim, Young-Suk;Jung, Ji-Young;Ha, Yeong-Lae;Yang, Jae-Kyung
    • Journal of Life Science
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    • v.21 no.8
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    • pp.1190-1198
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    • 2011
  • Extraction methods of medicinal plants were evaluated for growth enhancing effects of human neuroblastoma SK-N-SH cells. Hot-water extraction (reflux for 5 hr), hot-water extraction post treatment (100$^{\circ}C$ or 120$^{\circ}C$, 90 min) and ethanol extraction (reflux for 5 hr) methods were applied to Angelica gigas, Rhemania glutinosa, Paeonia lactiflora and Cnidium officinale samples to extract their constituents. Cells were treated for 2 hr with various concentrations of extracts (0, 0.5, 1, 2, 4 ${\mu}g/{\mu}l}$ media) prior to $H_2O_2$ (250 ${\mu}M$) treatment for 2 hr to provide oxidative stress. Cell viability, caspase-3 expression and apoptosis were measured for cells treated with sample extracts. Hot-water extract exhibited a stronger growth enhancing and apoptosis protecting ability than other extracts. These activities were shown at less than 1 ${\mu}g/{\mu}l}$ concentration, and not greater than 2 ${\mu}g/{\mu}l}$ concentration. Hot-water extract contained more polyphenolic compounds than other extracts coming along with stronger antioxidant activity. The efficacy of antioxidant activity was stronger in the hot-water extract of Angelica gigas than other hot-water extracts of medicinal plants. These results suggest that hot-water extraction is an appropriate method to extract materials for growth enhancing and apoptosis protection of SK-N-SH cells, and hot-water extracts of Angelica gigas might be useful materials for protection from aging brain cells.

Natural herbal extract complex induces the degradation of alcohol and acetaldehyde and reduces the breath alcohol concentration (천연소재복합물의 알코올 및 아세트알데히드 분해활성 및 호기중 알코올 농도 감소)

  • Hwang, Ji Hong;Kim, Mi-Yeon
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.3
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    • pp.381-392
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    • 2020
  • This study is purposed to check up the natural 12 kinds of herbal extracts suitable for hangover and based on the results of contents of phenolic compounds, ABTS radical scavenging activity, alcohol dehydrogenase (ADH), and acetaldehyde dehydrogenase (ALDH). Selected 8 kinds of herbal extracts are blended according to the efficacy and the pearson's correlation between each content and activity. C. sinensis var. sinensis, P. densiflora Gnarl and P. lobata Ohwi showed excellent ADH activity, P. lobata Ohwi had a strong correlation between the content and efficacy, and C. sinensis var. sinensis, P. densiflora Gnarl had a negative correlation. Through the ADH and ALDH activity test of F.1 to F.7, the F.7 showed the highest synergic effect and selected as an optimal formulation. F.7 intake-group, the breath alcohol concentration was significantly reduced to 58% after 30 minutes and 27% after 120 minutes, compared to right after alcohol consumption. After alcohol consumption, there was a significant improvement effect (p<0.05) in tired and thirst in the intake group compared to the non-intake group.

Characteristics of Microwave-Assisted Extraction for Grape Seed Components with Different Solvents (추출용매에 따른 포도씨 성분의 마이크로웨이브 추출 특성)

  • Lee Eun-Jin;Kwon Joong-Ho
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.216-222
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    • 2006
  • Microwave energy (2450 MHz) was applied to extract health-effective component (HEC) from grape seed. Three different solvents approved for grape seed extract such as water, ethanol and acetone, were confirmed in their microwave-heating properties and by which extraction efficiencies were determined. Microwave-assisted extraction (MAE) was performed under different parameters; microwave power : (0, 50, 100 and 150), time : (1, 3, 5, 7, and 9 min), the sample to solvent ratio : (1:20, 1:10, 1:5 and 1:2.5), and particle size(whole, 20, 40 and 60 mesh) and the subsequent extracts were used for determining their physicochemical properties, such as total yield : (TY), total phenolics : (TP), catechin content : (CC), electron donating ability : (EDA), and browning color. The heating properties of solvent demonstrated the optimal ranges of microwave and time as 100 W and 2 to 6 min. The TY and HFC content were higher with increasing powers in water and ethanol solvent, while HFC content were lower in acetone at over 100 W. The longer of extraction time up to 5 min, the higher extraction efficiency. Based upon the overall MAE efficiency and solvent recovery, it was found optimal to use 10 times volume of ethanol for 10 mesh of seed particle at 100 W.

Hot-water Extraction Condition of Gastrodia elata Blume by Response Surface Methodology (반응표면분석법을 이용한 천마의 열수 추출조건 설정)

  • Kim Seong-Ho;Kim In-Ho;Kang Bok-Hee;Lee Sang-Han;Kim Jong-Hyun;Lee Jin-Man
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.131-137
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    • 2006
  • In order to reduce the damage of effective component during processing, we carried out hot-water extraction monitering in Gastrodia elata Blume by response surface methodology. The extraction con야tions for vanillyl alcohol, vanillin and coumarin were optimized at 91.07, 93.73 and $96.30^{\circ}C$, for 2.64, 2.58 and 3.84 hr, at the concentration of 15.16, 21.56 and 19.88 mL/g, respectively. The optimal condition for extraction of total phenolics was acheved at $98.14^{\circ}C$, 3.20 hr and 18.63 mL/g. This study elicited regression formula for each variable, and superimposed the total optimal conditions of extraction for effective compounds and optimal condition for antioxidant characteristics dividing processing factors. It is shown that the predicted result under the extraction condition as follows: extraction temperature for $90{\sim}100^{\circ}C$, extraction time for $3{\sim}4hr$, and solvent volume of $15{\sim}20mL/g$ of sample.

An Analysis of Phenolic Compounds, Carbon Isotopes, and Sugar Components of Whiskys Based on the Period of Maturation (위스키의 종류와 숙성기간에 따른 페놀성 화합물, 탄소동위원소비 및 당 성분 분석)

  • Cho, Eun-Ah;Lee, Young-Sang
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.57-63
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    • 2012
  • This paper analyzes phenolic compounds, carbon isotopes, and sugar components of whiskys based on the maturation period. For this, the paper considers a total of 40 whiskys(mainly imports) distributed in Korea. It is important to analyze the presence phenolic compounds(e.g., furfural, syringaldehyde, vanillin, syringic acid, and vanillic acid) because these are found only in whiskys ripened in oak. The results indicate that the total content of phenolic compounds increased with the increase in the storage period regardless of the type of whisky. In terms of vanillin/syringaldehyde(V/S), Scotch whiskys had 0.4~0.5; American whiskies, 0.30~0.34; and Canadian whiskies, 0.31~0.33. In terms of Scotch whiskys, Macallan had 0.25~0.34, making it unique among Scotch whiskys. In terms of the ratio of carbon isotopes, there were clear differences between malt Scotch whiskys, blended Scotch grain whiskys, American whiskys, and Canadian whiskys: -23.4~ -24.3, -16.8~-21.0, -11.0~-11.5 and -9.5~13.9, respectively. In addition, malt Scotch whiskys contained 40~230 $mg/{\ell}$ of fructose; blended Scotch whiskys, 20~120 $mg/{\ell}$; American whiskys, 50~70 $mg/{\ell}$; and Canadian whiskys, 20~100 $mg/{\ell}$, demonstrating that the fructose content of single-malt whiskys was twice the average fructose content. On the other hand, malt Scotch whiskys contained 30~170 $mg/{\ell}$ of glucose; blended Scotch whiskys, 20~120 $mg/{\ell}$; American whiskys, 20~30 $mg/{\ell}$; and Canadian whiskys, 10~110 $mg/{\ell}$, demonstrating that the glucose content of single-malt whiskys exceeded the average glucose content. This study's results can be used as a database of classification for whiskys based on the fermentation of raw ingredients and the period of maturation for distinguishing between different types of whiskys. In addition, the results can facilitate the verification of genuine whiskys by allowing for the identification of different types of whiskys based on the period of maturation.

Physicochemical Characteristics and Antioxidant Activities of Luffa cylindrica (L.) Roem (수세미오이의 이화학적 특성과 항산화활성)

  • Lee, Gyu-Ok;You, Yang-Hee;Hwang, Kwon-Tack;Lee, Jeong-Min;Lee, Ho-Joon;Jun, Woo-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.6
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    • pp.733-738
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    • 2012
  • Physiochemical characteristics of Luffa cylindrica (L.) Roem and antioxidant activities of its four extracts were analyzed. The chemical composition of L. Roem contained 93.69% moisture, 1.64% crude fiber, 0.52% crude protein, 0.27% crude fat, and 0.06% crude ash. The major minerals were potassium, phosphrous, and calcium. We also analyzed the major organic acids, acetic acid and succinic acid. In free amino acids, ${\beta}$-aminoisobutyric acid showed the highest concentration (100.74 mg/100 g), followed by phosphoethanolamine, urea, asparagine, and valine. Glutamic acid was the highest hydrolyzed amino acid with 1,039.99 mg/100 g followed by aspartic acid, lysine, and arginine. Four extracts from L. Roam, hot-water (LCH), cold-water (LCC), 80% ethanol (LCE), and methanol (LCM), were prepared. Total phenolic and flavonoid levels of LCE exhibited higher than three extracts. The antioxidant potential of extracts from L. Roem were investigated using DPPH, ABTS, and NBT assays. Of the four extracts, LCE had relatively high antioxidant capability on DPPH and superoxide anion radical scavenging activities (12.13%, 16.88%, and 26.61%). Based on the above results, it is suggested that an 80% ethanolic extract from L. Roem was a natural antioxidant material for health food and should be a good ingredient for functional food.

Quality of Sponge Cakes Supplemented with Cinnamon (계피분말을 첨가한 스펀지케이크의 품질특성)

  • Lee, Subin;Lee, Jun Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.4
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    • pp.650-654
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    • 2013
  • The feasibility of incorporating cinnamon as a value-added food ingredient in bakery food products was investigated using sponge cakes as a model system. Cinnamon powder was incorporated into sponge cakes at 0, 1, 2, 3, or 4% weight amounts, based on the total weight of wheat flour. Cake qualities, such as pH, moisture content, specific volume, and baking loss, decreased significantly with increasing levels of cinnamon powder added (p<0.05). In terms of color, lightness and yellowness decreased, with increasing levels of cinnamon powder. Redness significantly increased for crumb, but decreased for crust (p<0.05) with increasing levels of cinnamon powder. Hardness also significantly increased (p<0.05), while total polyphenol content significantly increased, up to a 2% addition of cinnamon powder, and gradually increased afterwards. Finally, the consumer acceptance test has indicated that higher levels of cinnamon incorporation (2% or higher, w/w) have a considerable adverse effect on consumer preferences in all attributes. In contrast, sponges cakes with a minimal level of cinnamon powder (1%, w/w) are recommended for taking advantage of the functional properties of cinnamon powder without sacrificing consumer acceptability.

Study on the Anti-oxidant Effect of Pinus rigida Mill. inner Bark Extracts (리기다소나무(Pinus rigida Mill.) 내수피 추출물의 항산화효과에 관한 연구)

  • Jang, Min-Jung;An, Bong-Jeun;Lee, Chang-Eeon;Lee, Jin-Tae;Lee, Byung-Guen;Lee, Do-Hyung
    • Journal of Korean Society of Forest Science
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    • v.97 no.1
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    • pp.88-94
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    • 2008
  • The aim of the study was to assess the cosmeceutical activity of Pinus rigida Mill. and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. The concentration of total phenolic compound of P. rigida water soluble fraction and P. rigida ethyl acetate soluble fraction showed 47 mg/L and 601 mg/L respectively. In the result of DPPH scavenging radical activity, P. rigida ethyl acetate soluble fraction showed 86% and it was similar to BHA effect at 10 ppm concentration. Xanthine oxidase inhibition of P. rigida water soluble fraction and P. rigida ethyl acetate soluble fraction were 76.3% and 80.5% at 500 ppm, respectively. In the result of tyrosinase inhibition effect related to skin-whitening, P. rigida water and ethyl acetate soluble fraction showed 42%, 10.9% at a 1,000 ppm. In these results, P. rigida has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.