• Title/Summary/Keyword: 특징값추출

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Postmortem Changes of the Protein and Amino Acid Composition of Muscles in the Partially Frozen Prawn, Pandalus japonica (보리새우육의 부분동결저장중 단백질 및 아미노산의 조성변화)

  • PYEUN Jae-Hyeung;CHOI Young-Joon;KIM Jeung-Han;CHO Kweon-Ock
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.4
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    • pp.280-290
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    • 1984
  • An extensive study has been made on the relationship between the freshness and the compositions of the muscle protein of prawn, Pandalus japonica during the storage under partially frozen condition. The variations of the subunit distribution for sarcoplasmic protein and myofibrillar protein extracted from the samples by changes of freshness were discussed by sodium dodecylsulfate-poly-acrylamide gel (SDS-PAG) electrophoresis. On the other hand, the denaturation constant ($K_D$) of the myofibrillar protein extracted from the prawn stored at $-3^{\circ}C\;and\;-20^{\circ}C$ were successively compared. The prawn muscle contained about $18\%$ of protein with the composition of $32\%$ in sarcoplasmic protein, $56\%$ in myofibrillar protein, $10\%$ in residual intracellular protein and $2\%$ in stroma. The indices for estimating freshness of the muscle were approached to the early stage of putrefaction on the 26th day of the storage with $25.29mg\%$ of total volatile basic nitrogen, $31.36\%$ of K-value and 8.83 of pH. The content of the myofibrillar protein was remarkably decreased with the time during the storage while that of residual intracellular protein was increased. The $K_D$ values of the myofibrillar protein were $9.03{\times}10^{-6}sec^{-1}\;at\;-3^{\circ}C\;and\;4.42{\times}10^{-6}sec^{-1}\;at\;-20^{\circ}C$. The results of the analysis of SDS-PAG electrophoretograms indicated that the sarcoplasmic protein and the myofibrillar protein were composed of 12 subunits and 17 subunits in the muscle of instantaneously killed prawn ana were changed into 8 subunits and 22 subunits in the muscle stored for 26 days, respectively. It is noticeable that 30,000, 41,000, 107,000, 136,000, 170,000 173,000, 185,000, and 198,000 daltons of the newly appeared 8 subunits were found in the myofibrillar protein from the prawn muscle stored for 26 days. The amino acid composition of the muscle protein showed that the most of amino acids were slightly decreased with the days of the storage. With respect to the free amino acid composition of the muscle of instantaneously killed prawn, glycine, proline, arginine, alanine and taurine comprised $93\%$ of the total free amino acids. Taurine, valine, leucine, phenylalanine, serine, lysine, methionine, isoleucine and histidine were increased during the storage period but exceptionally proline was decreased.

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Petroleum Geochemistry of Organic Matter from the core samples in the Tertiary Pohang Basin (포항 분지 제3기층 시추코아 유기물의 석유 지화학적 특성)

  • Lee Youngjoo;Kwak Young Hoon;Yun Hye Su;Cheong Tae Jin;Oh Jae Ho;Kim Hagju;Kang Moohee
    • The Korean Journal of Petroleum Geology
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    • v.5 no.1_2 s.6
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    • pp.48-58
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    • 1997
  • Core samples from the B, E, F, H wells in the Tertiary Pohang Basin were analysed for total organic carbon (TOC) content and subject to Rock-Eval pyrolysis in order to assess petroleum geochemical characteristics of organic matter. Following geochemical screening, we selected samples from each well for the study of bitumen and kerogens such as optical observation, infra-red spectroscopy and biomarker analyses. Sediments of the Tertiary Yonil Group contain total organic carbon ranging from $0.55{\%} to 3.74{\%}$ with S1+S2 values higher than 2mgHC/g Rock in B, E and F wells, which indicates fair hydrocarbon generation potential. Most organic matter in the B, E, F wells is compared to type II based on the Rock-Eval pyrolysis, infra-red spectroscopy and optical observation. However, organic matter in the H well is compared to type III because the well is located at the margin of the basin where the preservation of terrestrial material is dominant. Geochemical analyses show that organic matter in the Yonil Group is thermally immature although thermal maturity slightly increases with depth. Maturity levels of the extracted kerogens are similar to those of bulk samples ($Tmax<435^{\circ}C$. Petroleum geochemical charateristics of the sediments in the Tertairy Yonil Group is fair in terms of the organic richness and hydrocarbon genetic potential, but organic matter is thermally immature due to the shallow burial depth. Optical observation of the kerogens and biomarker analysis show that organic matter in the Yonil Group is both marine and terrestrial origin, although it was deposited in marine environment. Pristane/phytane ratio suggests rather anoxic depositional environment. Transitional characteristics of organic matter indicate that the marine Yonil Group was deposited near the terrestrial environments. Input of terrestrial organic matter is more prevalent in the samples recovered from the lowermost horizon in the wells due to the terrestrial environment at the time of basin formation.

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A Study on Developing Sensibility Model for Visual Display (시각 디스플레이에서의 감성 모형 개발 -움직임과 색을 중심으로-)

  • 임은영;조경자;한광희
    • Korean Journal of Cognitive Science
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    • v.15 no.2
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    • pp.1-15
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    • 2004
  • The structure of sensibility from motion was developed for the purpose of understanding relationship between sensibilities and physical factors to apply it to dynamic visual display. Seventy adjectives were collected by assessing adequacy to express sensibilities from motion and reporting sensibilities recalled from dynamic displays with achromatic color. Various motion displays with a moving single dot were rated according to the degree of sensibility corresponding to each adjective, on the basis of the Semantic Differential (SD) method. The results of assessment were analyzed by means of the factor analysis to reduce 70 words into 19 fundamental sensibilities from motion. The Multidimensional Scaling (MDS) technique constructed the sensibility space in motion, in which 19 sensibilities were scattered with two dimensions, active-passive and bright-dark Motion types systemically varied in kinematic factors were placed on the two-dimensional space of motion sensibility, in order to analyze important variables affecting sensibility from motion. Patterns of placement indicate that speed and both of cycle and amplitude in trajectories tend to partially determine sensibility. Although color and motion affected sensibility according to the in dimensions, it seemed that combination of motion and color made each have dominant effect individually in a certain sensibility dimension, motion to active-passive and color to bright-dark.

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The Weather Representativeness in Korea Established by the Information Theory (정보이론에 의한 한국의 일기대표성 설정)

  • Park, Hyun-Wook
    • Journal of the Korean association of regional geographers
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    • v.2 no.2
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    • pp.49-73
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    • 1996
  • This study produces quantitatively weather entropy and information ratio using information theory about frequency in the appearance of precipitation phenomenon and monthly change, and then applies them to observation of the change of their space scale by time. As a result of these, this study defines Pusan, Chongju and Kwangju's weather representativeness and then establishes the range of weather representativeness. Based on weather entropy (statistical parameter)-the amount of average weather information-and information ratio, we can define each area's weather representativeness, which can show us more constant form included topographical, geographical factors and season change. The data used for this study are the daily precipitotion and cloudiness during the recent five years($1990{\sim}1994$) at the 69 stations in Korea. It is divided into class of no precipitation, that of precipitation. The results of this study can be summarized as follows: (1) The four season's mean value of information ratio is the highest value. as 0.641, on the basis of Chongju. It is the lowest as 0.572, on the basis of Pusan. On a seasonal basis, the highest mean value of information rate is April's (spring) in Chongju, and the lowest is October's(fall) in Pusan. Accordingly weather representativeness has the highest in Chongju and the lowest in Pusan. (2) To synthesize information ratio of decaying tendancy and half-decay distance, Chonju's weather representativeness has the highest in April, July and October. And kwangju has the highest value in January and the lowest in April and July. Pusan's weather representativeness is not high, that of Pusan's October is the lowest in the year. (3) If we establish the weather representative character on the basis of Chongju-Pusan, the domain of Chongju area is larger than that of Pusan area in October, July and April in order. But Pusan's is larger than Chongju's in January. In the case of Chongju and Kwangju, the domain of Chongju area is larger than that of Kwangju in October, July and April in order, but it is less than that of Kwangju area in January. In the case of Kwangju-Pusan, the domain of Kwangju is larger than that of Pusan in October, July in order. But in April it is less than Pusan's.

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Characteristics of EMG Median Frequency and Torque During Isometric Back Extension Exercises (등척성 요추 신전운동 시 중앙주파수와 토크의 특성)

  • Kang, S. J.;Park, S. J.;Jang, K.;Park, K. H.;Kwon, O. Y.;Kim, Y. H.
    • Journal of Biomedical Engineering Research
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    • v.23 no.1
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    • pp.9-16
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    • 2002
  • Localized muscle fatigue can be identified by a downward shift of the EMG frequency typically represented by a fall in the median frequency The Present experimental study was Performed to investigate the time change of the median frequency and the muscle torque during maximal isometric back extension exercises at different exercise angles (0$^{\circ}$, 12$^{\circ}$, 36$^{\circ}$and 72$^{\circ}$) Twenty heath subjects (mean age : 24.35 $\pm$ 2.70) were Participated in this study Median frequency was extracted from EMG signals by employing the fast Fourier transform. Initial median frequency and the slope of median frequency was not significantly correlated with the muscle torque. Pearson's Product moment correlation was used to quantify the relationship between slopes of median frequency and torque. The results may suggest that the exorcise angle during maximal isometric back extension exercises does not affect the slopes of the median frequency and torque, and y-intercept of the median frequency among exercise angles There was no significant correlation between slopes of median frequency and torque. But there was a moderate correlation between median frequency and torque at each exercise angle. In conclusion, the exercise angle during maximal isometric back extension exercise is not a direct effect on slopes of median frequency and torque. But results showed that the shift of median frequency and torque shift were highly correlated in all subjects.

Extraction of Water Body Area using Micro Satellite SAR: A Case Study of the Daecheng Dam of South korea (초소형 SAR 위성을 활용한 수체면적 추출: 대청댐 유역 대상)

  • PARK, Jongsoo;KANG, Ki-Mook;HWANG, Eui-Ho
    • Journal of the Korean Association of Geographic Information Studies
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    • v.24 no.4
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    • pp.41-54
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    • 2021
  • It is very essential to estimate the water body area using remote exploration for water resource management, analysis and prediction of water disaster damage. Hydrophysical detection using satellites has been mainly performed on large satellites equipped with optical and SAR sensors. However, due to the long repeat cycle, there is a limitation that timely utilization is impossible in the event of a disaster/disaster. With the recent active development of Micro satellites, it has served as an opportunity to overcome the limitations of time resolution centered on existing large satellites. The Micro satellites currently in active operation are ICEYE in Finland and Capella satellites in the United States, and are operated in the form of clusters for earth observation purposes. Due to clustering operation, it has a short revisit cycle and high resolution and has the advantage of being able to observe regardless of weather or day and night with the SAR sensor mounted. In this study, the operation status and characteristics of micro satellites were described, and the water area estimation technology optimized for micro SAR satellite images was applied to the Daecheong Dam basin on the Korean Peninsula. In addition, accuracy verification was performed based on the reference value of the water generated from the optical satellite Sentinel-2 satellite as a reference. In the case of the Capella satellite, the smallest difference in area was shown, and it was confirmed that all three images showed high correlation. Through the results of this study, it was confirmed that despite the low NESZ of Micro satellites, it is possible to estimate the water area, and it is believed that the limitations of water resource/water disaster monitoring using existing large SAR satellites can be overcome.

Gene Expression Profiles in Cervical Cancer with Radiation Therapy Alone and Chemo-radiation Therapy (자궁경부암의 방사선치료 및 방사선항암화학 병용치료에 따른 유전자발현 조절양상)

  • Lee Kyu Chan;Kim Meyoung-kon;Kim Jooyoung;Hwang You Jin;Choi Myung Sun;Kim Chul Yong
    • Radiation Oncology Journal
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    • v.21 no.1
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    • pp.54-65
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    • 2003
  • Purpose : To analyze the gene expression Profiles of uterine ceulcal cancer, and its variation after radiation therapy, with or without concurrent chemotherapy, using a CDNA microarray. Materials and Methods :Sixteen patients, 8 with squamous ceil carcinomas of the uterine cervix, who were treated with radiation alone, and the other 8 treated w14h concurrent chemo-radiation, were Included in the study. Before the starling of the treatment, tumor biopsies were carried out, and the second time biopsies were peformed after a radiation dose of 16.2$\~$27 Gy. Three normal cervix tissues were used as a control group. The microarray experiments were peformed with 5 groups of the total RNAs extracted individually and then admixed as control, pre-radiation therapy alone, during-radiation therapy alone, pre-chemoradiation therapy, and during-chemoradlation therapy. The 33P-iabeled CDNAS were synthesized from the total RNAs of each group, by reverse transcription, and then they were hybridized to the CDNA microarray membrane. The gene expression of each microarrays was captured by the intensity of each spot produced by the radioactive isotopes. The pixels per spot were counted with an Arrayguage, and were exported to Microsoft Excel The data were normalized by the Z transformation, and the comparisons were peformed on the Z-ratio values calculated. Results : The expressions of 15 genes, including integrin linked kinase (ILK), CDC28 protein kinase 2, Spry 2, and ERK 3, were increased with the Z-ratio values of over 2.0 for the cervix cancer tissues compared to those for the normal controls. Those genes were involved In cell growth and proliferation, cell cycle control, or signal transduction. The expressions of the other 6 genes, Including G protein coupled receptor kinase 5, were decreased with the Z-ratio values of below -2.0. After the radiation thorapy, most of the genes, with a previously Increase expressions, represented the decreased expression profiles, and the genes, with the Z-ratio values of over 2.0, were cyclic nucleotlde gated channel and 3 Expressed sequence tags (EST). In the concurrent chemo-radiation group, the genes involved in cell growth and proliferation, cell cycle control, and signal transduction were shown to have increased expressions compared to the radiation therapy alone group. The expressions of genes involved in anglogenesis (angiopoietln-2), immune reactions (formyl peptide receptor-iike 1), and DNA repair (CAMP phosphodiesterase) were increased, however, the expression of gene involved In apoptosls (death associated protein kinase) was decreased. Conclusion : The different kinds of genes involved in the development and progression of cervical cancer were identified with the CDNA microarray, and the proposed theory is that the proliferation signal stalls with ILK, and is amplified with Spry 2 and MAPK signaling, and the cellular mitoses are Increased with the increased expression oi Cdc 2 and cell division kinases. After the radiation therapy, the expression profiles demonstrated 4he evidence of the decreased cancer cell proliferation. There was no sigificant difference in the morphological findings of cell death between the radiation therapy aione and the chemo-radiation groups In the second time biopsy specimen, however, the gene expression profiles were markedly different, and the mechanism at the molecular level needs further study.

Development and evaluation of Pre-Parenthood Education Program for high school students based on Home Economics subject (고등학생을 위한 가정교과 기반 예비부모교육 프로그램 개발 및 평가)

  • Noh, Heui-Yeon;Cho, Jae Soon;Chae, Jung Hyun
    • Journal of Korean Home Economics Education Association
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    • v.29 no.4
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    • pp.161-193
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    • 2017
  • The purpose of this study was to develop and evaluate pre-parenthood education program(PPEP) based on Home Economics(HE) subject for high school students. The development and evaluation of PPEP based on HE subject in this study followed ADDIE model except implementation through 4 processes such as analysis, design, development, and evaluation. First, program development directions were set in three aspects such as 'general development', 'contents', and 'teaching and learning methods'. Themes of the program are 11 in total such as '1. Parenting, what is being a parent', '2. Choosing your spouse, happy marital relationship, the best gift to your children', '3. Pregnancy and birth, a moving meeting with a new life', '4. Taking care of a new born infant for 24 hours', '5. Taking care of infants, relationship with my lovely baby, attachment', '6. Taking care of young children, my child from another planet', '7. Parents and children in healthy family', '8. Parent-child relationship, wise parents to make effective interaction with their children', '9. Parents safety manager at home,', '10. Practice to take care of infants', and '11. Practice of community nurturing support service development'. In particular, learning activities of the program have major characteristics such as 1) utilization of cases including practice problems related to parenting, 2) community exchange activities utilizing learned knowledge and techniques, 3) actual life project activities utilizing learning contents related with parenting, 4) activities inducing positive changes in current life of high school students, and 5) practice activities for the necessities of life such as food, clothing and shelter supporting development of children. Second, the program was developed according to the design. Teaching-learning plans and materials for 17 classes were developed according to 11 themes. The developed plans include class flow and teacher's reference. It starts with receiving a class-related message from a virtual child at the introduction stage and ended with replying to the message by summarizing contents of the class and making a promise as a parent-to-be. That is the basic frame of class flow. Learning materials included various plans and reports necessary for learning activities and they are prepared in details so that they can be play the role of textbooks in regular curriculum. Third, evaluation of developed program was executed by a 5 point Likert scale survey on 13 HE experts on two aspects of program development process and program development results. In the evaluation of development process, mean value was 4.61 and index of content validity was 97.4%. For development results, mean value was 4.37 and index of content validity was 86.9%. These values showed that validity in the development process and results in this study was highly secured and confirmed that PPEP based on HE was appropriate and valid to enhance parent qualifications of high school learners.