• Title/Summary/Keyword: 특성인자

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Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite loci in Domestic Beef Cattle (초위성체 DNA표지인자를 이용한 국내 육우집단의 품종특성 및 개체식별 체계설정)

  • Kim, Sang-Wook;Jang, Hee-Kyung;Kim, Kwan-Suk;Kim, Jong-Joo;Jeon, Jin-Tae;Yoon, Du-Hak;Kang, Seong-Ho;Jung, Hyo-Il;Cheong, Il-Cheong
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.273-282
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    • 2009
  • DNA marker information is used to identify or distinguish cattle breeds or individual animal. The purpose of this study was to apply Bovine Genotypes Kit Version 1.1/2.1 to bovine DNA samples (National Institute of Animal Science) taken from Australian / American beef (n=148), Holstein beef (n=170) and Hanwoo cattle (n=177) bred in Jeongeub, Jeonbuk, Korea, so that it could distinguish Hanwoo breed. The Bovine Genotype Kits consist of 16 ISAG MS markers, which were used to build a database of genotypes in each group. Genotyping results were analyzed using MS Tool kit and Phylip program to create phylogenetic tree. The GeneClass 2.0 was used to estimate breed identification. These analyses found that this kit had 100% capacity to distinguish Hanwoo beef, 95.3% capacity to differentiate Australian / American beef and 90% capacity to identify Korean Holstein steer beef. Hence, it is expected that 16 commercial microsatellite markers is useful to categorizegenetic characteristics of Hanwoo breed and also identify Hanwoo individuals and the origin of beef. In particular, it is expected that these markers will be advantageous in discriminating domestic Holstein beef from Australian / Americanbeef.

Determination of Main Indicator for the Changes of Chemical Properties in Greenhouse Soils (시설재배지 토양의 화학적 특성변화에 영향을 미치는 주요 인자 선발)

  • Yoon, Young-Eun;Kim, Jang Hwan;Kim, Song Yeob;Im, Jong Uk;Kong, Myung Suk;Lee, Young Han;Lee, Young Bok
    • Korean Journal of Environmental Agriculture
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    • v.34 no.4
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    • pp.355-358
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    • 2015
  • BACKGROUND: Changes in Korea's agricultural soil properties were analyzed at a four-year interval from 1999 to 2002 on a national scale and used as basis for the determination of the appropriate agricultural policy on maintaining food safety and soil quality. The scope of this study ideally requires sampling thousands of paddy, greenhouse, upland and orchard land across the country, however, due to limitations in economic and manpower resources, it was deemed necessary to reduce sampling site to greenhouse soil. In this study, we try to investigate the applicability of cultivated crops as criteria for selecting representing fields in greenhouse soils.METHODS AND RESULTS: Soil samples were collected from red pepper, oriental melon, watermelon and strawberry cultivated soil. Principal components analysis (PCA) was performed on soil chemical properties of the selected fields: pH, electron conductivity (EC), available phosphate (Av-P2O5), organic matter (OM), and exchangeable cation (Ex.-K, Ca, and Mg). Soil chemical properties of oriental melon cultivated soil was separated from red pepper, watermelon, and strawberry cultivated soil on PC1 and red pepper cultivated soil was separated from watermelon cultivated soil on PC2. Position on PC1 was strongly correlated with pH, Ex.-Ca and Ex.-Mg and position on PC2 was strongly correlated with OM and Av-P2O5.CONCLUSION: The soil chemical properties of greenhouse soil was assorted amongst the different crops. Therefore, the cultivated crops as a criteria for the selection of representative field in greenhouse soil would be used in the future.

Program Development to Evaluate Permeability Tensor of Fractured Media Using Borehole Televiewer and BIPS Images and an Assessment of Feasibility of the Program on Field Sites (시추공 텔리뷰어 및 BIPS의 영상자료 해석을 통한 파쇄매질의 투수율텐서 계산 프로그램 개발 및 현장 적용성 평가)

  • 구민호;이동우;원경식
    • The Journal of Engineering Geology
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    • v.9 no.3
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    • pp.187-206
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    • 1999
  • A computer program to numerically predict the permeability tensor of fractured rocks is developed using information on discontinuities which Borehole Televiewer and Borehole Image Processing System (BIPS) provide. It uses orientation and thickness of a large number of discontinuities as input data, and calculates relative values of the 9 elements consisting of the permeability tensor by the formulation based on the EPM model, which regards a fractured rock as a homogeneous, anisotropic porous medium. In order to assess feasibility of the program on field sites, the numerically calculated tensor was obtained using BIPS logs and compared to the results of pumping test conducted in the boreholes of the study area. The degree of horizontal anisotropy and the direction of maximum horizontal permeability are 2.8 and $N77^{\circ}CE$, respectively, determined from the pumping test data, while 3.0 and $N63^{\circ}CE$ from the numerical analysis by the developed program. Disagreement between two analyses, especially for the principal direction of anisotropy, seems to be caused by problems in analyzing the pumping test data, in applicability of the EPM model and the cubic law, and in simplified relationship between the crack size and aperture. Aside from these problems, consideration of hydraulic parameters characterizing roughness of cracks and infilling materials seems to be required to improve feasibility of the proposed program. Three-dimensional assessment of its feasibility on field sites can be accomplished by conducting a series of cross-hole packer tests consisting of an injecting well and a monitoring well at close distance.

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Quality Indicators of End-of-Life Cancer Care from the Family Members' Perspective in Korea (호스피스 임상 질 지표에 대한 보호자의 동의정도와 이와 관련된 인자)

  • Kim, Hyo-Min;Youn, Chang-Ho;Ko, Hae-Jin
    • Journal of Hospice and Palliative Care
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    • v.14 no.2
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    • pp.101-109
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    • 2011
  • Purpose: The Assessing Care Of Vulnerable Elders project has developed indicators for the quality of the end-of-life (EOL) care. However, family members of cancer patients may have a different view on the quality indicators (QIs) established by health care experts. We evaluated the QIs from the family members' perspective. Methods: The information used in this study was gathered by surveying family members of cancer patients who were admitted to the inpatient hospice ward in Korea. A cross-sectional anonymous questionnaire was distributed to 120 potential respondents from July to October 2009. We included 69 responses in this study (effective response rate, 57.5%). Statistical analysis was performed by using SPSS for Win ver. 14.0, with independent t-test and Pearson's chi-square test. Results: Among QIs for good hospice care, families agreed the most on "Medical examination by the palliative care team or specialist" (88.4%) and "Dying in the family's presence" (88.4%). They agreed the least on "Discussing cardiopulmonary resuscitation with patients" (15.9%). Among QIs for bad hospice care, "Occurrence of fall or pressure ulcer are undesirable" showed the highest agreement rate (94.2%). The lowest agreement rate was 11.6% on "Dying in the hospital is undesirable." In addition, women, patients who were aware of their prognoses, and people with a high socioeconomic status tended to agree more on the QIs. Conclusion: Patients' families did not agree on some of the QIs recommended by previous studies. Family members' characteristics were related to their opinions on QIs.

A Study on Pulping Process Condition through Design of Experiments (실험계획법을 이용한 고지 해리 공정조건에 관한 연구)

  • Kim, Sung-Kwon;Lee, Joon-Koo;Sung, Dae-Hyung
    • Applied Chemistry for Engineering
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    • v.9 no.4
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    • pp.529-535
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    • 1998
  • This study was made to investigate the effect of variation in pulping process conditions such as wastepaper blending treatments, temperature, and chemical blending treatments on the deinked pulp. Design of experiments was used to select the major factors which significantly influenced on the deinked pulp. As one of the statistical analysis technique, analysis of variance and multiple comparison technique was used to find the best process condition and the predicted values and confidence intervals for brightness and strength were obtained. In the condition of $Na_2SiO_3$ 2.0%, $H_2O_2$ 0.5% and wastepaper blending treatments (KONP : AONP : OMG = 40 : 30 : 30 wt %) the highest brightness of 50.5% was predicted with 90% confidence interval (49.0, 52.0). On a concentration of $H_2O_2$ 0.5%, the highest tensile index of $35.7N{\cdot}m/g$ was predicted with 90% confidence interval (34.6, 36.6) at the pulping temperature of $50^{\circ}C$. On a concentration of surfactant 0.1%, the highest burst index of $0.129kPa{\cdot}m^2/g$ was predicted with 90% confidence interval (0.125, 0.133) at the pulping temperature of $50^{\circ}C$.

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Chlorophyll Content and Genetic Variation of Ginkgo biloba L. Planted on the Street in Seoul (도심지 은행나무 가로수의 엽록소 함량 및 유전변이 특성)

  • 김판기;구영본;이재천;배상원;이용섭;정용문
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.3 no.2
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    • pp.114-120
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    • 2001
  • Ginkgo biloba L. has been planted in the city as street trees because reported as resistant species to air pollutant. Especially, the trees planted on the street of 'Cheongro', Mt. 'Nam', and 'Jamsil' have been exposed to air pollutant for a long time. This study was conducted to examine chlorophyll contents and genetic variation of Ginkgo biloba in the areas. Chlorophyll contents measured in the above three areas were variable although the the diameter at breast height measured in 'Cheongro' and Mt. 'Nam' were constant. In addition, the result showed positive relation between chlorophyll contents and DBH in this study. Eight enzyme systems were analyzed in megagametophytes which were collected in the areas and separated to two groups based on chlorophyll contents. All the enzymes appeared to be polymorphic : Got-2, Pgi-2, Pgm, Acon, Mnr, Mdh, Skdh, and 6Pgd. The sensitive (S) groups varied from 1.253 to 2.571 in the genetic diversity and the tolerant (T) groups ranged from 1.416 to 2.825. The observed single locus heterozygosities (H$_{0}$) ranged from 0.056 to 0.611 in the S groups, and from 0.179 to 1.643 in the T groups. The expected heterozygosities (H$_{e}$) ranged from 0.208 to 0.629 in the S groups and from 0.321 to 0.658 in the T groups. In addition, the H$_{0}$ values averaged over all loci were 0.326 for the T groups and 0.299 for the S group, respectively. A difference between the two groups was 0.027. The T groups had the unique alleles and genotypes and all the parameters for genetic diversity showed that the T groups had higher genetic diversity than the S groups.s. genetic diversity than the S groups.

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Effects of NaCl/H3PO4 Flame Retardant Treatment on Lyocell Fiber for Thermal Stability and Anti-oxidation Properties (NaCl/H3PO4 내염화 처리가 라이오셀 섬유의 열 안정 및 내산화 특성에 미치는 영향)

  • Kim, Eun Ae;Bai, Byong Chol;Jeon, Young-Pyo;Lee, Chul Wee;Lee, Young-Seak;In, Se Jin;Im, Ji Sun
    • Applied Chemistry for Engineering
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    • v.25 no.4
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    • pp.418-424
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    • 2014
  • The improved thermal stability and anti-oxidation properties of Lyocell fiber were studied based on flame retardant treatment by using NaCl/$H_3PO_4$ solution. The optimized conditions of flame retardant treatment were studied on various maxing ratio of NaCl and $H_3PO_4$ and the mechanism was proposed through experimental results of thermal stability anti-oxidation. The IPDT (integral procedural decomposition temperature), LOI (limited oxygen index) and $E_a$ (activation energy) increased 23, 30 and 24% respectively via flame retardant treatment. It is noted that thermal stability and anti-oxidation improved based on char and carbon layer formation by dehydrogenation and dissociation of C-C bond resulting the hindrance of oxygen and heat energy into polymer resin. The optimized conditions for efficient flame retardant property of Lyocell fiber were provided using NaCl/$H_3PO_4$ solution and the mechanism was also studied based on experimental results such as IDT (initial decomposition temperature), IPDT, LOI and $E_a$.

Novel Variations in Human Interleukin-29 and Their Association (사람의 Interleukin-29 유전자의 새로운 변이의 단리 및 그들의 연관)

  • Song, Ju-Hee;Chae, Soo-Cheon;Lee, Jae-Hoon;Chung, Hun-Taeg
    • Journal of Life Science
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    • v.14 no.2
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    • pp.275-279
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    • 2004
  • Gene polymorphisms of cytokines and their receptors are attractive candidates as genetic factors in the pathogenesis of immune-mediated diseases and have been reported to be associated with disease susceptibility to autoimmune, inflammatory and infectious diseases. IL-29 is one of important candidate genes for complex trait of genetic diseases but there is no published survey of single nucleotide polymorphisms (SNPs) in this gene. In this study, for the first time, we have examined the full genomic sequence of IL-29 including the promoter regions to identify SNPs. We examined the frequencies of genotypes and alleles at the SNP site of IL-29 in allergic rhinitis patients and non-allergic rhinitis controls using the direct sequencing method to determine whether this IL-29 SNP is associated with allergic rhinitis in Korean population. We identified one novel SNP (1184C>A) in the intron 2 and one novel variation site (-1842_-1841dupGA) in the promoter region of human IL-29 gene. The P values of SNP or variation site were not significant between the healthy controls and allergic rhinitis patients. Our results suggest that the 1184C>A polymorphism and -1842_-1841dupGA variation site in human IL-29 gene were not associated to allergic rhinitis.

An Experimental Study on the Application of LIBS for the Diagnosis of Concrete Deterioration (콘크리트 열화 진단의 LIBS 적용을 위한 실험적 연구)

  • Woo, Sang-Kyun;Chu, In-Yeop;Youn, Byong-Don
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.21 no.6
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    • pp.140-146
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    • 2017
  • It is laser induced breakdown spectroscopy(LIBS) that enables qualitative and quantitative analysis of the elements contained in unknown specimen by comparing the wavelength characteristics of each element obtained from the spectral analysis of the standard specimen with the wavelength analysis results from unknown specimens. In this study, the applicability of LIBS to the analysis of major deterioration factors affecting concrete durability was experimentally analyzed. That is, the possibility of applying LIBS to the diagnosis of concrete deterioration by studying the quantitative detection of harmful deteriorating factors on chloride, sulfate and carbonated mortar specimens using LIBS was studied. As a result of LIBS test for each chloride and sulfate specimen, the LIBS spectral wavelength intensity of chlorine and sulfur ions increased linearly with increasing concentration. Carbon ion LIBS spectral wave intensities of carbonated specimens increased nonlinearly over the duration of carbonation exposure. From the above results, it can be partially confirmed that LIBS can be applied to the diagnosis of concrete deterioration. In case of concrete carbonation, it is presumed that carbon content is contained in the cement itself and is different from the detection of chloride and sulfate specimen. Therefore, it is considered that more various parameter studies should be performed to apply LIBS to concrete carbonation.

Effects of Gamma Irradiation and Methyl Bromide Fumigation on Quarantine Pest and Physical Quality of Apples (감마선과 methyl bromide 처리가 사과의 검역해충 및 물리적 품질에 미치는 영향)

  • Kang, Ho-Jin;Kwon, Yong-Jung;Byun, Myung-Woo;Kim, Hyun-Ku;Chung, Hun-Sik;Choi, Jong-Uook;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.783-790
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    • 2003
  • Tetranychus urticae Koch and Panonychus ulmi Koch were identified as quarantine pests in apple samples and were controlled soon after the commercial treatment of methyl bromide (MeBr) and with irradiation doses $(0.5{\sim}3kGy)$ after irradiation. The physical qualities of apples, such as rotting, withering, weight changes, hardness, and color, were apparently affected by MeBr. Furthermore, more than 2 kGy irradiation resulted from the lapse of storage time. Associated with the physical qualities of stored apples, irradiation and fumigation for quarantine purposes were more adequate after 40 days of storage at $0{\pm}1^{\circ}C\;(85{\pm}2%\;RH)$ immediately following harvest, rather than soon after harvest. Less than 1 kGy irradiation, based on its accumulated control effect on pests, can potentially be applied as a quarantine procedure without causing significant changes in the physical qualities of fresh apples, in contrast to MeBr.