• Journal of Applied Biological Chemistry
• /
• v.55 no.3
• /
• pp.173-178
• /
• 2012

Sucrose-glucan glucosyltransferase (Gtf) is an important enzyme involved in the cavity formation process where insoluble glucan is synthesized. In this study, we purified Gtf from Streptcoccus mutans Ingbritt through ammonium sulfate precipitation, Sephadex G-150, CM-Sephadex, and DEAE-Sephadex column chromatographies. A 13-fold of purification was achieved with a total yield of 6.3%. The apparent molecular mass of the enzyme was determined to be 66 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal pH and temperature were established to be 6.0 and $40^{\circ}C$, respectively. The enzyme activity could be inhibited to 22-59% by 1 mM $Hg^{2+}$, $Cu^{2+}$ and $Al^{3+}$, and to 68% by 1 mM EDTA. It was also inhibited 40% by 2 mM xylitol and 35-45% by 0.05% soluble chitosan, glycol chitosan, and glycol chitin. This is the first report to reveal the inhibition effect of chitin derivatives on Gtf activity, which may be further applicable to develop gargles to overcome cavity.

• Investigative Magnetic Resonance Imaging
• /
• v.11 no.2
• /
• pp.103-109
• /
• 2007

To develop an advanced non-linear curve fitting (NLCF) algorithm for performing dynamic susceptibility contrast study of the brain. The first pass effects give rise to spuriously high estimates of $K^{trans}$ for the voxels that represent the large vascular components. An explicit threshold value was used to reject voxels. The blood perfusion and volume estimation were accurately evaluated in the $T2^*$-weighted dynamic contrast enhanced (DCE)-MR images. From each of the recalculated parameters, a perfusion weighted image was outlined by using the modified non-linear curve fitting algorithm. The present study demonstrated an improvement of an estimation of the kinetic parameters from the DCE $T2^*$-weighted magnetic resonance imaging data with using contrast agents.

• Investigative Magnetic Resonance Imaging
• /
• v.15 no.1
• /
• pp.11-21
• /
• 2011

Purpose : Localization using MRI is difficult due to susceptibility induced artifacts caused by metal electrodes. Here we took an advantage of the B0 pattern induced by the metal electrodes by using an oblique-view imaging method. Materials and Methods : Metal electrode models with various diameters and susceptibilities were simulated to understand the aspect of field distortion. We set localization criteria for a turbo spin-echo (TSE) sequence usingconventional ($90^{\circ}$ view) and $45^{\circ}$ oblique-view imaging method through simulation of images with various resolutions and validated the criteria usingphantom images acquired by a 3.0T clinical MRI system. For a gradient-refocused echo (GRE) sequence, which is relatively more sensitive to field inhomogeneity, we used phase images to find the center of electrode. Results : There was least field inhomogeneity along the $45^{\circ}$ line that penetrated the center of the electrode. Therefore, our criteria for the TSE sequence with $45^{\circ}$ oblique-view was coincided regardless of susceptibility. And with $45^{\circ}$ oblique-view angle images, pixel shifts were bidirectional so we can detect the location of electrodes even in low resolution. For the GRE sequence, the $45^{\circ}$ oblique-view anglemethod madethe lines where field polarity changes become coincident to the Cartesian grid so the localization of the center coordinates was more facilitated. Conclusion : We suggested the method for accurate localization of electrode using $45^{\circ}$ oblique-view angle imaging. It is expected to be a novelmethodto monitoring an electrophysiological brain study and brain neurosurgery.

• Applied Biological Chemistry
• /
• v.18 no.3
• /
• pp.167-176
• /
• 1975

As a study on the ATP-inhibited ribonuclease of Bacillus subtilis the screening work for obtaining the ATP-inhibited ribonuclease negative mutant were carried out. And mutant strain was selected by the treatment of N-methyl-N'-nitro-N-nitrosoguanidine (NTG). For the selected strain the enzyme purification and some physiological properties were examined and the results obtained were as follows. 1. Among tested 1817 strains with the treatment of NTG, 101 strain was selected as a mutant strain. 2. ATP-inhibited ribonuclease was tentatively purified by several independent column chromatography. The results with Sephadex G-75 column were 30 times purification, 99% recovery, and 20 times purification, 98% recovery, respectively. 3. ATP-inhibited ribonuclease was purified by 60 times through acid treatment, ammonium fractionation, and two successive chromatography. 4. The purified ribonuclease were shown to be effectively concentrated in robonnclease content and to have reduced numbers of protein band on Disc electrophoresis. 5. This enzyme degraded single-stranded RNA to 2',3'-cyclic AMP, 2',3'-cyclic CMP, 2',3,-cyclic GMP, 2',3'-cyclic UMP and some unknown intermediates. The enzyme could not split double-stranded RNA.

• Korean Journal of Optics and Photonics
• /
• v.19 no.2
• /
• pp.150-158
• /
• 2008

The exact transmission coefficients at the interface between a uniaxial anisotropic medium and an isotropic medium at? oblique incidence are derived by applying the extended Jones matrix method. When the birefringence of the uniaxial anisotropic medium is small ($|n_e-n_o|\;{\ll}\;n_o,\;n_e$), the exact transmission coefficients are compared with those by the conventional extended Jones matrix method by Yeh et al. They showed an excellent agreement with each other. In addition, using the exact transmission coefficients, we calculated the polarization characteristics of a light through a uniaxial medium to an incident light with arbitrary polarization state at? oblique incidence. We compared the transmittances of an unpolarized light through a pair of crossed o-type polarizers by two different methods and calculated the transmittance as the variation of the optical constants of the polarizers to evaluate of the extinction ratio. The polarization analysis method using the exact transmission coefficients can be applied to polarization characteristics of a light through a uniaxial medium with large birefringence as well as to liquid crystals and to optical anisotropic material.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.47 no.5
• /
• pp.62-73
• /
• 2010

In this paper we proposed a candidate algorithm on G.711.1 and G.722 superwideband extension codec which is under standardization by ITU-T. The proposed codec not only provides an interoperable bitstream with ITU-T G.711.1 and G.722, but also encodes a superwideband signal with a bandwidth of 50-14,000 Hz using superwideband extension layer. The candidate codec consists of a core layer to provide an interoperability with conventional wideband codecs and superwideband extension layer using linear prediction-based sinusoidal coding. The proposed extension codec operates on 5ms frame and provides four superwideband bitrates of 64, 80, 96, and 112 kbit/s depending on the core codec. Since the resulting bitstream has an embedded structure, it can be converted into core bitstream by simple truncation without transcoding. The proposed codec has a short algorithmic delay and low complexity and passed the qualification test of G.711.1 and G.722 superwideband extension codec performed by ITU-T.

• Journal of KIISE:Computer Systems and Theory
• /
• v.37 no.3
• /
• pp.184-193
• /
• 2010

This paper proposes a biological iridescence BRDF(Bidirectional Reflectance Distribution Function) compression and rendering method. In the graphics technology, iridescence sometimes is named structure colors. The main features of these symptoms are shown transform of color and brightness by varying viewpoint. Graphics technology to render this is the BRDF technology. The BRDF methods enable realistic representation of varying view direction, but it requires a lot of computing power because of large data. In this paper, we obtain reflection map from iridescence BRDF, analyze color of reflection map and propose representation method by several colorfully concentric circle. The one concentric circle represents beam width of reflection ray by one normal vector. In this paper, we synthesize rough concentric by using several virtually optical normal vectors. And we obtain spectrum information from concentric circles passing through the center point. The proposed method enables IBR(image based rendering) technique which results is realistic illuminance and spectrum distribution by one texture from reduced BRDF data within spectrum.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.19 no.3
• /
• pp.194-204
• /
• 2007

A numerical analysis and hydraulic experiments were undertaken to investigate the head loss occurring when a flow passes through vertical perforated walls. The numerical analysis applied continuity, momentum and energy equations to the control volumes that were set near the perforated wall. Non-dimensional equations were then derived to calculate both upstream depth and head loss for the given values of downstream depth and velocity. The hydraulic experiments were performed with several single and triple perforated plates varying their opening ratios and intervals. The numerical results with the single plates were compared with the experimental results, and it was shown that the contraction coefficient of the vertical line jet formed after the perforated plates relies on downstream Froude number as well as opening ratio. Based on the experimental results, empirical formulas were formulated. Finally, the formulas were applied to the triple plates sequentially from downstream side to upstream side, and it was found that in general the predicted values nicely agreed with the experimental results.

• Proceedings of the KSR Conference
• /
• 2007.05a
• /
• pp.786-791
• /
• 2007

Korean high speed train(HSR-350x) has adopted a combined electrical and mechanical(friction) braking system. Brake blending control unit(BBCU) controls each brake system to fulfill the required brake performances such as braking distance, deceleration and jerk. Also the braking system should be designed considering the economical management, such as effective use of generated braking energy and the minimum wear of friction materials(a pad and a brake shoe). In this paper, we establish the disc braking force pattern that reduces the wear of pad in the disc braking system by minimizing the variance of the instantaneous disk baking energy during braking time, and compare the wear mass of pad between the conventional disc braking force pattern and the established results.

• Journal of Navigation and Port Research
• /
• v.34 no.3
• /
• pp.175-180
• /
• 2010

In the almost application parts, GNSS being used the primary navigation system on world-widely. However, some of nations attempt or deliberate to enhance current Loran system, as a backup to satellite navigation system because of the vulnerability to the disturbance signal. Loran interests in supplemental navigation system by the development and enhancement, which is called eLoran, and that consists of advancement of receiver and transmitter and of differential Loran in order to increase the accuracy of current Loran-C. A significant factor limiting the ranging accuracy of the eLoran signal is the ASF in the TOAs observed by the receiver. The ASF is mostly due to the fact that the ground-wave signal is likely to propagate over paths of varying conductivity and topography. This paper presents comparison results between the predicted ASF and the measured ASF in a southern east region of Korea. For predicting ASF, the Monteath model is used. Actual ASF is measured from the legacy Loran signal transmitted Pohang station in the GRI 9930 chain. The test results showed the repeatability of the measured ASF and the consistent characteristics between the predicted and the measured ASF values.