• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1038-1042
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• 2007

수변완충지대는 수역과 육역의 경계에 위치하면서 하도를 따라 선형으로 길게 연속적으로 이어지는 공간으로서 토양, 동식물 등 생태시스템을 이루는 자연공간이다. 수변완충지대의 기능은 일반적으로 수질정화, 생태통로 및 서식처, 강턱의 안정, 토양침식 방지, 경관 개선 등이며 하천의 일부로서 사회적 경제적으로 높은 가치를 지니고 있다. 특히 최근에는 비점오염 저감과 하천 생태서식처 조성분야에서의 적용이 활발히 검토되고 있다. 선진국에서는 1980년대 이후부터 수변완충지대의 중요성을 인식하고 환경 생태 치수 측면에서 효율적 조성 및 복원 방법에 관한 연구와 적용이 본격적으로 시작되었고 가이드라인이 제시되었다. 이 분야에서 기술적 정책적으로 가장 앞선 나라로 평가되고 있는 미국의 경우 정부에서는 농무부(USDA)를 비롯하여 환경처(EPA), 내무부(USDI), 공병단(US ARMY)의 관련 산하기관, 각 주정부의 담당부서에서 수변완충지대의 보전과 조성에 관련된 여러 가지 정책과 기준을 제시하고 있다. 이들 가이드라인에서는 수변완충지대가 필요로 하는 기능별 최소 폭, 식생 종별 구성, 식생배열 등을 제시하며 그 기준은 조성 목적이나 기능, 대상 지역별로 차이를 보인다. 기능별 최소 폭은 수질정화를 목적으로 하는 경우 $4m{\sim}61m$이상, 생태서식처 조성을 목적으로 하는 경우는 $30m{\sim}1,000m$ 이상을 권장하고 있다. 식생 종은 수변완충지대 폭을 상 중 하단부로 나누어 초본, 관목, 교목류로 식재하고 강우유출수의 유입부에서 초본을 통한 부유고형물 등을 여과하고, 목본류의 뿌리와 토양을 통해 질소, 인 등 영양물질을 저감하는 구조를 제시하고 있다. 미국의 경우는 이러한 가이드라인을 실제로 현장에 적용하고 있으며 조성효과가 정성적으로 검증되었고 정량적인 관계 도출을 위한 연구가 지속적으로 진행되고 있다. 국내의 경우 수질개선 및 생태서식처 조성, 하천환경을 개선하기 위한 관련 정책이 꾸준히 제시되고 있다. 한 예로 정부에서는 수변구역을 지정 고시하여 상수원으로 유입되는 비점오염물질을 저감하고 생태서식처를 조성함으로써 양질의 상수원과 건전한 생태계를 확보하기 위한 제도를 시행 중이다. 수변 구역의 범위는 한강수변구역의 경우 약 $500{\sim}1,000m$로 설정되어 있으나 아직까지는 관리적 성격이 강하며, 향후에는 구체적인 조성방안이 요구된다. 본 연구에서는 한강 수변구역 내에 미국의 가이드라인에서 제시하는 수변완충지대의 물리적 조건과 국내 지역 토착식생을 적용하여 시험완충지대를 조성하였고, 실험조건 하에서 그 효과를 정량적으로 분석 검증한 결과와 외국사례를 분석하여 국내여건에 맞는 수변완충지대 조성 가이드라인(안)을 제시하였다.

• Korean Journal of Microbiology
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• v.51 no.2
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• pp.169-176
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• 2015

The malolactic fermentation (MLF), which is widely used in winemaking, is the conversion of malic acid to lactic acid conducted by the malolactic enzyme (Mle) of lactic acid bacteria. In order to select the strains with MLF among 54 lactic acid bacteria isolated from the traditionally fermented foods, we designed a primer set that specifically targets the conserved regions of the mle gene and then selected four strains that harbor the mle gene of Lactobacillus plantarum. All strains were identified as L. plantarum by analyzing the 16S rRNA sequences, biochemical properties, and the PCR products of the recA gene. From comparison of the mle gene sequences consisting of 1,644 bp, the nucleotide and amino acid sequence of strain JBE60 correspond to 96.7% and 99.5% with those of other three strains, respectively. The strain JBE60 showed the highest resistant against 10% (v/v) ethanol among the strains. The strains lowered the concentration of malic acid to average 43%. Considering the ethanol resistance and conversion of malic acid, the strain JBE60 is considered as a potential starter for the malolactic fermentation.

• Korean Journal of Microbiology
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• v.48 no.3
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• pp.192-199
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• 2012

Microalgal biotechnology has gained prominence because of the ability of microalgae to produce value-added products including biodiesel through photosynthesis. However, carbon and nutrient source is often a limiting factor for microalgal growth leading to higher input costs for sufficient biomass production. Use of municipal wastewater as a low cost alternative to grow microalgae as well as to treat the same has been demonstrated in this study using mini raceway open ponds. Municipal wastewater was collected after primary treatment and microalgae indigenous in the wastewater were encouraged to grow in open raceways under optimum conditions. The mean removal efficiencies of TN, TP, COD-$_{Mn}$, $NH_3$-N after 6 days of retention time was 80.18%, 63.56%, 76.34%, and 96.74% respectively. The 18S rRNA gene analysis of the community revealed the presence of Chlorella vulgaris and Scenedesmus obliquus as the dominant microalgae. In addition, 16S rRNA gene analysis demonstrated that Rhodobacter, Luteimonas, Porphyrobacter, Agrobacterium, and Thauera were present along with the microalgae. From these results, it is concluded that microalgae could be used to effectively treat municipal wastewater without aerobic treatment, which incurs additional energy costs. In addition, municipal wastewater shall also serve as an excellent carbon and nitrogen source for microalgal growth. Moreover, the microalgal biomass shall be utilized for commercial purposes.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.2
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• pp.100-116
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• 2002

There are several purpose to introduce microorganism into the Soil. The major purpose is to promote plant growth and inhibit plant pathogens. The model example is to put in nitrogen fixing symbiotic bacteria, Pythium and Rhizobium. In order to achieve the intended goal, the introduced microorganism should survive and colonize with sufficient density. The survival of introduced microorganism depend upon biotic and abiotic factors. Predation and competition are important among biotic factor. Water tension, organic carbon, inorganic nutrients(N, P), pH are important factor among abiootic factor. Soil texture and distribution of soil pore are also important in the survival and colonization of introduced microorganism. Selection by soil ecosystem for inoculant is a crucial factor for colonization. Good example are control of autochtonous microorganism and the introduction of surfactant biodegrading Pseudomonas. Sometimes, carriers such as peat and montmorillonite can be added to help colonization. Carriers can protect introduced microorganism by supplying protective microhabitat. Organic polymer is also used as a carrier to immobilize bacteria or industrial enzymes. Examples of these carrier are calcium alginate, agarose and k-carrageenan. The function of these carrier is to provide microhabitat and help colonization for introduced microorganism.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.7
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• pp.757-763
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• 2006

In our previous studies, we have isolated bacteria from BTEX-contaminated sediment, which utilized BTEX as a sole carbon source and $NO_3$-N as an electron acceptor. For the possibility of field application, we have applied co-culture of those isolates in the BTEX-contaminated soil and evaluated their biodegradation efficiencies. To investigate the relationship between the isolates and indigenous microorganism in soil, changes of microbial community structure in soil samples with respect to time were monitored. To examine this, soil samples were artificially contaminated with benzene, toluene, ethylbenzene and o-xylene. BTEX-degrading bacteria such as Pseudomonas stutzeri strain 15(DQ 202712), Klebsiells sp. strain 20(DQ 202715) and Citrobacter sp. strain A(DQ 202713) were injected into the soil samples in the ratio of 2:1:1. Our results showed that the highest BTEX biodegradation efficiency was achieved when both BTEX and $NO_3-N$ existed simultaneously. The change in soil microbial community structure was characterized by PCR-DGGE analysis comparing the relative DGGE band intensities. The band intensities of indigenous microorganisms in the soil were reduced by injecting co-culture of the three isolates. On the contrary, the relative band intensities of the isolates were increased. Among the three isolates, Pseudomonas stutzeri strain 15 rendered the highest band intensity. This indicates that the Pseudomonas stutzeri was the dominant microbial species found in the soil samples.

• Korean journal of applied entomology
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• v.54 no.4
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• pp.295-301
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• 2015

We investigated mycophagous ladybird, Illeis koebelei from 12 species of plants infected with powdery mildew in Gyeonggi-do, Korea. The pear tree, Pyrus ussuriensis var. macrostipes (Nakai), was most preferred by I. koebelei. This species was found from early July to early November in pear orchards. There was no entomophagous trace in the gut of I. koebelei without powdery mildew spores in a microscope. All stages except egg and pupa are obligate mycophagous, and the feeding potential is ranked as follows: fourth instar, adults, third instar, second instar, and first instar. Feeding amounts of each stage of I. koebelei were 45.6, 144.4, 372.2, 628.1, and $473.7mm^2$ of cucumber powdery mildew per day. Fourth instar larvae showed highest consumption of cucumber powdery mildew. Developmental periods of four larval instars and adults feeding cucumber powdery mildew were 1.2, 2.3, 2.3, 4.6, and 37.7 days, respectively, at $25^{\circ}C$. In this study, we could not determine the feeding potential of I. koebelei against the cucumber powdery mildew; therefore, and further studies are required to elucidate the potential of this species as a biological control agent, e.g., mass rearing, selection of low toxic chemical agents for Integrated Pest Management (IPM), and control techniques against powdery mildew in agro-ecosystems.

• Journal of Life Science
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• v.22 no.9
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• pp.1194-1200
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• 2012

Lactic acid bacteria are generally recognized as beneficial probiotic organisms. Recent studies revealed that the potential of probiotic strains was essentially dependent on the bacterial-binding and adhesion capabilities to gut epithelial cells and the hydrophobicity of the cell surface. In this study, we screened some indigenous lactic acid bacteria from Kimchi and selected one lactic acid bacterium as a potential probiotic based on its cell surface hydrophobicity. Analysis of the 16S rRNA gene sequences of probiotic isolates indicated that the selected isolate (BCNU 9070 strain) was a member of Pediococcus pentosaceus. P. pentosaceus BCNU 9070 showed resistance to bile acids and acidic pH. The P. pentosaceus BCNU 9070 strain also inhibited the cell growth of six food-borne pathogens including Listeria monocytogenes and Shigella sonnei. In addition, the P. pentosaceus BCNU 9070 strain expressed bile salt hydrolase activity and showed an ability to assimilate cholesterol in vitro. On the basis of these results, P. pentosaceus BCNU 9070 is considered to have probiotic potential for applications in functional foodstuffs.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.354-361
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• 2009

This study was carried out to develop an effective biocarrier-mediated bioaugmentation technology which will be useful for remediation of the crude oil-contaminated marine sediments. Enrichment of several microbial communities was made from several oil-polluted seashore sites and the two distinctively functional consortia have been successfully selected. These two consortia were grown together and used to manufacture the microbial agents for bioaugmentation of marine sediments polluted with crude oil. The most dominant species in the mixed culture was identified as Alcanivorax borkumensis based on pure culture and DGGE analysis. Bioaugmentation of oil-polluted marine sediments with the microbial agent MA-2 formulated using the mixed culture and biocarriers (activated carbon and minerals) was more effective, especially in combination with an oxygen producing (releasing) compound (ORC). Ninty percent of TPH was removed in the presence of ORC in 35 days while 74% in the absence of ORC. This indicated that the indigenous consortial degraders could be immobilized on the active carbon as a biocarrier to manufacture microbial agents and then effectively bioaugmented for remediation of the oil-polluted sediments.

• Applied Biological Chemistry
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• v.39 no.5
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• pp.403-408
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• 1996

Total bacterial community DNA, which was extracted from microcosm soil and field soil after 2,4-D amendments, was analyzed on Southern blots, using the tfdA gene probe derived from plasmid pJP4 and the Spa probe from Sphingomonas paucimobilis. Southern blot analyses with total bacterial DNA extracted from soils Inoculated with Pseudomonas cepacia/pJP4 revealed that DNA probe method could detect the 2,4-D degrading bacteria down to $10^5\;cells/g$ dry soil. In the microcosm experiment, there was a good correlation between 2,4-D degradation and banding patterns in hybridization analyses performed after each 2,4-D treatment using the two probes. When bacterial DNA extracted from microcosm soil was hybridized with the Spa probe, a change in the position of hybrid bands was observed over time in a Southern blot, suggesting that population change or possibly genetic rearrangement in 2,4-D degrading microbial populations occurred in this soil. With the Spa probe, one hybrid DNA band was persistently observed throughout the five 2,4-D additions. When bacterial DNA isolated from the field soil was probed with the tfdA and Spa, strong hybridization signal was observed in the 100 ppm-treated subplot, weak signal In the 10 ppm-treated subplot, and no significant signal in the 1 ppm-treated and control subplots. The data show that DNA probe analyses were capable of detecting and discriminating the indigenous 2,4-D degrading microbial populations in soil amended with 2,4-D under laboratory and field conditions.

• Journal of Korea Water Resources Association
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• v.54 no.11
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• pp.985-998
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• 2021

In general, the upstream dam changes downstream flow regime dramatically, i.e., from natural flow regime to hydropeaking flows. This study investigates the impact of the natural flow pattern on downstream fish habitat in a regulated river in Korea using the physical habitat simulation. The study area is a 13.4 km long reach of the Geum-gang River, located downstream from the Yongdam Dam, Korea. A field monitoring revealed that three fish species are dominant, namely Zacco platypus, Coreoleuciscus splendidus, and Opsariichthys bidens, and they account for 70% of the total fish community. Specially, Opsariichthys bidens is an indigenous species in the Geum-gang River. The three fish species are selected as target fish species for the physical habitat simulation. The Nays2D model, a 2D shallow water equation solver, and the HSI (Habitat Suitability Index) model are used for hydraulic and habitat simulations, respectively. To assess the impact of the natural flow pattern, this study uses the annual natural flow regime and hydropeaking flows from the dam. It is found that the natural flow regime increases significantly the Composite Suitability Index (CSI) in the study reach. Then, using the Building Block Approach (BBA), the scenarios for the modifying dam operations are presented in the study reach. Both Scenario 1 and scenario 2 are proposed by using the hydrological method considering both magnitude and duration of the inflow and averaging the inflow over each month, respectively. It is revealed that the natural flow regime embodied in scenario 1 and scenario 2 increases the Weighted Usable Area (WUA) significantly, compared to the hydropeaking flows. In conclusion, the modifying the dam operations by restoring to the natural flow pattern is advantageous to fish community.