• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.137-146
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• 2007

This study was conducted to determine fertilizer nutrient and pollutant production of Holstein dairy cattle by estimating manure characteristics. The moisture content of feces was 83.9% and 95.1% for urine. The pH of feces and urine were in the ranges of 7.0～7.4 and 7.5～7.8, respectively. The average BOD5, COD, SS, T-N, T-P concentrations of the dairy feces were 18,294, 52,765, 102,889, 2,575, and 457mg/ℓ, respectively. Dairy urine showed lower levels of BOD5(5,455mg/ℓ), COD(8,089mg/ℓ), SS(593mg/ℓ), T-N(3,401mg/l), and T-P(13mg/ℓ) than feces. The total daily produced pollutant amounts of a dairy cow were 924.1g(Milking cow), 538.8g(Dry cow), 284.4g(Heifer) of BOD5, 2,336.5g (Milking cow), 1,651.8g(Dry cow), 734.1g(Heifer) of COD and 4,210.1g(Milking cow), 2,417.1g(Dry cow), 1,629.1g(Heifer) of SS and 194.8g(Milking cow), 96.4g(Dry cow), 58.3g(Heifer) of T-N and 24.0g(Milking cow), 10.2g(Dry cow), 6.1g(Heifer) of T-P. The calculated amount of pollutants produced by a 450kg dairy cow for one year were 181.3kg of BOD5, 492.5kg of COD, 899.9kg of SS, 36.0kg of T-N and 4.1kg of T-P. The total yearly estimated pollutant production from all head(497,261) of dairy cattle in Korea is 90,149 tons of BOD5, 244,890 tons of COD, 447,491 tons of SS, 17,898 tons of T-N and 2,008 tons of T-P. The fertilizer nutrient concentrations of dairy feces was 0.26% N, 0.1% P2O5 and 0.14% K2O. Urine was found to contain 0.34% N, 0.003% of P2O5 and 0.31% K2O. The total daily fertilizer nutrients produced by dairy cattle were 197.4g (Milking cow), 97.4g(Dry cow), and 57.9g(Heifer) of Nitrogen, 54.2g(Milking cow), 22.2g(Dry cow), and 14.2g(Heifer) of P2O5 and 110.8g(Milking cow), 80.4g (Dry cow), and 39.5g(Heifer) of K2O. The total yearly estimated fertilizer nutrient produced by a 450kg dairy animal is 36.2kg of N, 8.8kg of P2O5, 24.6kg of K2O. The estimated yearly fertilizer nutrient production from all dairy cattle in Korea is 18,000 tons of N, 4,397 tons of P2O5, 12,206 tons of K2O. Dairy manure contains useful trace minerals for crops, such as CaO and MgO, which are contained in similar levels to commercial compost being sold in the domestic market. Concentrations of harmful trace minerals, such as As, Cd, Hg, Pb, Cr, Cu, Ni, Zn, met the Korea compost standard regulations, with some of these minerals being in undetected amounts.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.1
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• pp.9-20
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• 2007

This experiment was conducted to investigate effects of application of diluted and undiluted cattle slurry with water on seasonal and annual dry matter yields and feed values of tall fescue in the uncultivated rice paddy and it was compared with chemical fertilizer in order to determine optimal application season and dilution level of cattle slurry. When diluted or undiluted cattle slurry with water was applied to uncultivated rice paddy, annual dry matter yields showed 11.31 to 14.81 ton DM/ha (average 13.13 ton DM/ha) for diluted and 10.57 to 12.51 ton DM/ha (average 11.50 ton DM/ha) for undiluted cattle slurries, these had a higher dry matter yield than those of no fertilizer (9.21 ton DM/ha). Furthermore, separate application of early spring and summer (SA plots), separate application of early and late spring, and summer (SUA plots) fur undiluted cattle slurries, and whole application of spring (DS plots), separate application of early spring and summer (DSA plots), separate application of early and late spring, and summer (DSUA plots) for diluted cattle slurries were significantly (P<0.05) higher for annual dry matter yield than no fertilizer plots. Plots applied chemical fertilizer with nitrogen (N), phorphorus (P) and potassium (K) had 15.38 ton DM/ha annually, resulted in significantly (P<0.05) higher DM yield than chemical fertilizer containing P and K, and no fertilizer plots. Moreover, average annual DM yield for the chemical fertilizer with P and K was lower than that of cattle slurry applications. The efinciency of DM production for mineral nitrogen of chemical fertilizers was annually average 31.3 kg DM/kg N. In terms of cutting time of tall fescue, it was lowered in the order of 2nd growth followed by 1st and 3rd growth. However, efficiencies of annual DM production of nitrogen for diluted and undiluted cattle slurries were 26.1 and 15.3 kg DM/kg N, respectively, especially, highest in 2nd growth. While, efficiencies of DM production for cattle slurry versus for mineral nitrogen were 48.9 (undiluted) and 83.4% (diluted), respectively. For annual crude protein (CP) contents of tall fescue, aqueous cattle slurry applications showed 9,9 to 11.6%, which were significantly (P<0.05) higher than no fertilization (9.5%) and chemical fertilizer (9.0 to 9.8%), but annual average NDF and ADF contents were lowest in no fertilization. On the contrary, relative feed value (RFV) and total digestible nutrients (TDN) of no fertilizer plots were significantly (P<0.05) higher than the other plots. The application of cattle slurry and their dilution significantly increased yields of crude protein and total digestible nutrients compared with no and/or P and K fertilizers (P<0.05). These trends were much conspicuous in water-diluted cattle slurries applied in the early and late spring and summer, separately (DSUA plots).

• Applied Biological Chemistry
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• v.13 no.2
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• pp.111-129
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• 1970

As a series of studies on the nucleic acids and their related substances 210 samples were collected from 76 places such as farm soil, compost of heap, nuruk and meju to obtain microbial strains which produce 5'-phosphodiesterase. From these samples total of 758 strains were isolated by the use of dilution pour plate method. For all isolated strains primary screening of the productivity of RNA depolymerase was performed and useful strains with regard to 5'-phosphodiesterase productivities were identified. For these useful strains optimum condition, the effect of various compounds on the activity of 5'-phosphodiesterase, and the optimum condition for enzyme reaction were discussed. The quantitative of 5'-mononucleotides produced by the action of 5'-phosphodiesterase was performed using anion-exchange column chromatography and their identified was done by paper chromatography, thinlayer chromatography, ultra violet spectrophotometry, and characteristic color reaction using carbazole and schiff's reagent. (1) Penicillium citreo-viride PO 2-11 and Streptomyces aureus SOA 4-21 from soil were identified as a potent 5'-phosphodiesterase producing strains. (2) Optimum culture conditions for Penicillium citreo-viride PO 2-11 strain isolated were found to be pH 5.0 and $30^{\circ}C$, and the optimum conditions for enzyme action of 5'-phosphodiesterase were pH 4.2 and $60^{\circ}C$. Best carbon source for the production of 5'-phosphodiesterase was found to be sucrose and ammonium nitrate for nitrogen source. Addition of 0.01% corn steep liquor or yeast extract exhibited 20% increase in the amount of 5'-phosphodiesterase production compared to the control. 5'-phosphodiesterase produced by this strain was activated by $Mg^{++},\;Ca^{++},\;Zn^{++},\;Mn^{++}$ and was inhibited by EDTA, citrate, $Cu^{++},\;CO^{++}$. 5'-phosphodiesterase produced 5'-mononucleotide from RNA at a rate of 65.81%, and among the 5'-mononucleotides accumulated 5'-GMP only was found to have flavorous and the strain was also found lack of 5'-AMP deaminase. Productivity of flavorous 5'-GMP was found to be 186.7mg per gram of RNA. (3) Optimum culture canditions for the isolated Streptomyces aureus SOA 4-21 strain were pH 7.0 and $28^{\circ}C$, and the optimum conditions for the action of 5'-phosphodiesterase were pH 7.3 and $50^{\circ}C$. The best carbon source for 5'-phosphodiesterase production was found to be glucose and that of nitrogen was asparagine. Addition of 0.01% yeast extract exhibited increased productivity of 5'-phosphodiesterase by 40% compared to the non-added control. 5'-phosphodiesterase produced by this strain was activated by $Ca^{++},\;Zn^{++},\;Mn^{++}$ and was inhibited by citrate, EDTA, $Cu^{++}$. It was also found that the strain produce 5'-AMP deaminase in addition to 5'-phosphodiesterase. For this reason although decomposition rate was 63.58% the accumulation of 5'-AMP, 5'-CMP, 5'-GMP and 5'-UMP occurred by the breakdown of RNA. In the course of these reaction 5'-AMP deaminase converted 60% of 5'-AMP thus produced into 5'-IMP and flavorous 5'-mono nucleotide production was significantly increased by this strain over the above mentioned one. Production rates were found to be 171.8mg per grain of RNA for 5'-IMP and 148.2mg per gram of RNA for 5'-GMP, respectively.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.318-321
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• 2005

Forty- nine quarters from 24 lactating cows with chronic mastitis were selected. The cows were raised on dairy farms in Gongju, Jochiwon and Yeongi in Chungnam province, and Iksan in Jeonbuk province, Korea. The 49 quarters with bovine mastitis were divided into control (7 quarters) and experimental (42 quarters) groups. The experimental quarters were assigned to experimental group A (10 quarters, somatic cell count: $50-100\times10^4/ml)$, experimental group B (14 quarters, somatic cells count: $100-300{\times}10^4/ml)$, and experimental group C (18 quarters, somatic cells count: $>300\times10^4/ml$), according to the number of the somatic cells in their milk. The quarters of control group were treated with norfloxacin ointment (10 g/tube) based on the result of sensitivity, twice a day for 3 days. The quarters or experimental groups were infused 10ml or ozonated oils twice a day for 3 days. After treatment, the milk of the control group contained non-significantly lower numbers of somatic cells and bacteria on day 7, compared with pretreatment levels. Experimental groups A, B and C had lower somatic and bacterial cells in their milk on day 7, compared with pretreatment levels. Experimental group B and C had significantly lower numbers of somatic cells in their milk ell day 7 than before treatment (p<0.01). However, no significant difference in somatic cell numbers was detected between the control alld experimental groups. It was concluded that ozone therapy with ozonated oil applied on bovine mastitis might be effective.

• Applied Biological Chemistry
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• v.27
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• pp.29-46
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• 1984

To utilize several species of hard wood as raw materials of feed products, fermentation characteristics of cellulosic substrates to single cell protein was investigated, and results were summarized as follows. Among the microorganisms investigated, Tricoderma viride was selected as one of the most cellulolytic. Mixed culture of fungi did not show a synergistic effect on cellulose degradation. When the fungi were cultured at $28^{\circ}C$ for 7 days in a medium containing wheat bran 25 g, cellulose 0.25 g, proteose peptone 0.025 g and tween 800.025 g, cellulotic activities on carboxy methyl cellulose and filter paper reached maximum at 12 hr. The alkali treatment resulted in increased degradation of substrate from 13 to 18% when treated with enzymes for 12h, and reducing sugar formation increased with decreased size of substrates. Glucose was a very good feedback inhibitor of the enzyme from T.viride than that of xylose. When the substrate was rehydrolyzed, hydrolysis rate was 31% to reducing sugars within 12 hr. Quantative anlysis with HPLC showed the ratio of glucose to xylose in sugar syrups as 1.77 to 1. For the purpose of producing cellulosic-single cell protein from the sawdust of mulberry tree, 15 strains of xylose-assimilating yeast were isolated from 42 samples of rotten woods and compost soils and examined for their ability to utilize xylose. Then three strains were selected by their strong xylose-assimilating activities. The cultivative condition, the growth characteristics, and protein and nucleic acid productivities of three strains were investigated. The results obtained were, 1. Wood hydrolysate of mulberry tree was assimilated by 5 strains of CHS-2, CHS-3, ST-40, CHS-12 and CHS-13. 2. The optimum initial pH and temperature for the growth of strain CHS-13 were 4.4 and $30^{\circ}C$. 3. The specific growth rate of strain CHS-13 was $0.23h^{-1}$ and generation time was 3.01 hrs at the optimum condition. 4. CHS-13 strain assimilated 81 % of sugar in wood hydrolysate. 5. CHS-13 strain was identified as Candida guilliermondii var. guilliermondii 6. When the CHS-13 strain was cultured in the wood hydrolysate containing yeast extract, L-protein content was increased with yeast extract concentration. 7. The L-protein and nucleic acid yields from wood hydrolysate were 0.73 mg/ml and $4.92{\times}10^{-2}\;mg/ml$ respectively. 8. An optimal nucleic acid content of CHS-13 strain was observed in the medium containing 0.2% of yeast extract.

• Korean Journal of Organic Agriculture
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• v.16 no.1
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• pp.115-125
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• 2008

This study was conducted to determine adequate forage crop choice and optimal level of livestock manure, when different types and levels of the livestock manure were applied in corns or sorghum$\times$sorghum hybrids for the production of organic roughages by utilizing livestock manure. For the corn, yields of annual dry matter (DM) and total digestible nutrients (TDN) were highest in N+P+K-applied treatments, showing 17.3 and 11.7 ton/ha, respectively. Treatments applied 100% composted cattle manure (8.9 and 6.1 ton/ha) and 100% cattle slurry (9.4 and 7.5 ton/ ha) in contrast with chemical fertilizer-N had higher yields of DM and TDN than no fertilizer (4.8 and 2.7 ton/ha) and P+K-applied treatments (8.8 and 6.0 ton/ha). Particularly, treatments applied 150% composted cattle manure and 150% cattle slurry were markedly higher, which represented 11.4 and 7.6 ton/ha and 10.3 and 7.3 ton/ha, respectively. Crude protein (CP) contents for corns applied livestock manure ranged from 5.6 to 6.6%, which were significantly (p<0.05) higher than those of no fertilizer (3.9%) and P+K-applied treatments (5.5%). ADF (42.4%) and NDF (58.3%) contents for no fertilizer treatment were significantly (p<0.05) higher than those of other treatments. However, TDN contents were higher for livestock manure treatments than for no and/or chemical fertilizer treatments. In particular, TDN contents of treatments applied 150% composted cattle manure and 150% cattle slurry showed 72.3 and 70.8%, respectively and both treatments were significantly (p<0.05) higher than all of the other treatments. For the sorghum$\times$sorghum hybrid, yields of annual DM and TDN for 100% (12.4 and 7.4 ton/ha) and 150% (13.1 and 7.6 ton/ha) cattle slurry-applied treatments, and N+P+K-applied treatments (12.6 and 7.7 ton/ha) were significantly (p<0.05) higher than those of the others. In the others, 150% composted cattle manure (9.3 and 5.2 ton/ha) had higher annual DM and TDN yields than P+K-applied (8.4 and 4.8 ton/ha) and 100% composted cattle manure treatments (7.4 and 4.2 ton/ha), with no significant difference. Crude protein contents for sorghum$\times$sorghum hybrid applied P+K and cattle slurry were 8.8 and 8.6%, respectively. CP contents for both treatments were significantly higher than those of composted manure ($7.5{\sim}8.3%$) and no fertilizer (4.0%) treatments, but 100% livestock manure treatments had higher CP contents than 150%-applied treatments. ADF and NDF contents for N+P+K and cattle slurry-applied treatments were significantly (p<0.05) lower than the others. However, TDN contents were highest in N+P+K and cattle slurry-applied treatments, showing 61.2 and 58.3 to 59.4%, respectively. These results indicated that application of livestock manure instead of chemical fertilizer to the soil of forage crops might not only improve yields of DM and TDN, but also reduce environmental pollution by producing organic roughages through recycling of livestock manure.

• Korean Journal of Organic Agriculture
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• v.17 no.3
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• pp.371-380
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• 2009

This study was conducted to assess effects of supplying different types of nitrogen sources as fertilizers on productivity and feed values of barley and rye as winter forage crops, and ultimately done to get good quality of organic forages with higher fertilization of soil. For barley, N+P+K plots were significantly (P<0.05) higher in annual dry matter (DM) and total digestible nutrients (TDN) yields than other plots. However, cattle manure plots had significantly (P<0.05) higher annual DM and TDN than P+K and non-fertilizer plots. Plots of 50%-cattle manure and mixed sowing with hairy vetch or forage pea were higher than only 50%-cattle manure plot, particularly, these were significantly higher than non-fertilizer, and their crude protein (CP) yields were much higher than other plots. Crude protein contents were significantly higher in N+P+K and 50%-cattle manure slurry plots than non-fertilizer and P+K plots, and plots of mixed sowing with hairy vetch or forage pea in application of 50%-cattle manure had higher CP content than other plots. ADF content was lowest in 50%-cattle manure+forage pea plots, but highest in 100%-cattle manure plots. NDF content was lowest in legumes-mixed sowing, but highest in 100%-cattle manure plots. TDN content was the highest in forage pea plots, and plots of 50%-cattle manure and legumes-mixed sowing had high RFV, but cattle manure plots rich in ADF and NDF content had the lowest TDN and RFV. For rye, plots of 50%-cattle manure+hairy vetch mixed sowing, and N+P+K application had significantly higher annual DM, CP and TDN than other plots except for cattle manure. DM productive efficiency to nitrogen fertilization was markedly higher for cattle manure plots than for chemical fertilizer. This tendency was more conspicuous in plots of 50% cattle manure+legumes-mixed sowing. CP content was higher for N+P+K plot than for all plots, and plots of 50%-cattle manure + legumes-mixed sowing were significantly higher than other plots. On the contrary, forage pea-mixed sowing plot had the lowest ADF and NDF, but TDN and RFV were significantly (P<0.05) higher than other plots. Grass crop cultivation together with legumes by applying livestock manure to soil may lead to higher palatability of livestock, and better quality of forage. Furthermore, cattle manure application increased production yield per ha and CP contents. Thus, when applying forage crops produced by cattle manure application and mixed sowing to organic livestock production, it was conceived that forages produced might become a substitute for foreign organic grain as protein sources.

• Korean Journal of Organic Agriculture
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• v.16 no.4
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• pp.421-434
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• 2008

This study was carried out to estimate the selection of appropriate forage crops, proper application levels of livestock manure, and carrying capacity per unit area for organic livestock, as influenced by livestock manure application levels compared with chemical fertilizer to corn and sorghum $\times$ sorghum hybrid, in order to produce organic forages by utilizing livestock manure. For both corns and sorghum $\times$ sorghum hybrids, no fertilizer plots had significantly (p<0.05) lower annual dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) yields than those of other plots, whereas the N+P+K plots ranked the highest yields, followed by 150% cattle manure plots and 100% cattle manure plots. Dry matter, CP and TDN yields of cattle manure plots were significantly (p<0.05) higher than those of no fertilizer and P+K plots. In applying cattle manure, the yields of cattle slurry plots tended to be a little higher than those of composted cattle manure plots. Assuming that corns and sorghum $\times$ sorghum hybrids produced from this trial were fed at 70% level to 450kg of Hanwoo heifer with 400g of average daily gain, livestock carrying capacity (head/year/ha) ranked the highest in N+P+K plots of the case of corns (mean 6.7 heads), followed by 150% cattle slurry plots (mean 5.6 heads), 150% composted cattle manure plots (mean 4.8 heads), 100% cattle slurry plots (mean 4.4 heads), 100% composted cattle manure plots (mean 4.3 heads), P+K plots (mean 4.1 heads), and no fertilizer plots (mean 3.1 heads). Meanwhile, in case of sorghum $\times$ sorghum hybrids, N+P+K plots (mean 5.7 heads) ranked the highest carrying capacity, followed by $100{\sim}150%$ cattle slurry plots (mean $4.8{\sim}5.2$ heads), 150% composted cattle manure plots (mean 4.7 heads), 100 % composted cattle manure plots (mean 4.3 heads), P+K plots (mean 3.8 heads), and no fertilizer plots (mean 3.4 heads). The results indicated that replacing chemical fertilizer by livestock manure application to cultivation soil for forage crops could enhance not only DM and TDN yields, but also organic stock carrying capacity. In conclusion, it was conceived that organic forage production by reutilizing livestock manure might contribute to reduced environmental pollution and the production of environment friendly agricultural products through resources recycling.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.4
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• pp.329-339
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• 2021

Soybeans (Glycine max), one of major upland crops, require precise management of environmental conditions, such as temperature, water, and soil, during cultivation since they are sensitive to environmental changes. Application of spectral technologies that measure the physiological state of crops remotely has great potential for improving quality and productivity of the soybean by estimating yields, physiological stresses, and diseases. In this study, we developed and validated a soybean growth prediction model using multispectral imagery. We conducted a linear regression analysis between vegetation indices and soybean growth data (fresh weight and LAI) obtained at Miryang fields. The linear regression model was validated at Goesan fields. It was found that the model based on green ratio vegetation index (GRVI) had the greatest performance in prediction of fresh weight at the calibration stage (R²=0.74, RMSE=246 g/m², RE=34.2%). In the validation stage, RMSE and RE of the model were 392 g/m² and 32%, respectively. The errors of the model differed by cropping system, For example, RMSE and RE of model in single crop fields were 315 g/m² and 26%, respectively. On the other hand, the model had greater values of RMSE (381 g/m²) and RE (31%) in double crop fields. As a result of developing models for predicting a fresh weight into two years (2018+2020) with similar accumulated temperature (AT) in three years and a single year (2019) that was different from that AT, the prediction performance of a single year model was better than a two years model. Consequently, compared with those models divided by AT and a three years model, RMSE of a single crop fields were improved by about 29.1%. However, those of double crop fields decreased by about 19.6%. When environmental factors are used along with, spectral data, the reliability of soybean growth prediction can be achieved various environmental conditions.

• Korean journal of applied entomology
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• v.15 no.1 s.26
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• pp.7-16
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• 1976

In order to increase utility efficiency of red wood ants, Formica rufa truncicola var. yessonesis Forel as a resource of natural enemy of pine caterpillar, Dendrolimus spectabilis Butler, by finding out ecological and environmental factors in the habitat of red wood ants, the nest distribution and its density in habitat, plant distribution and density, stand-density of red pine, nest building and fixing plants, relative humidity of surface soil, physical and chemical natures of soil, and breeding rate were examined. The obtained results are summarized as follows: 1. The nest of red wood ants was densely distributed, in the lower-and middle top of mountain but no nest was found in the top. 2. The economical distribution of nest of habitat was estimated as $2.85/m^2$ and the lowest density as $1.93/m^2$ and these estimation lead us to confirm that pine caterpillar could be controlled. 3. The ecological characteristics of habitat seemed to be represented as higher stand-density of red pine of 10-20 years of age with large areas of eroded land under trees. The major grasses prevailing in this area were Andropogon brevifolius. Arundinella hirta, Miscanthus purpurasens, Eulia speciosa, Themeda japonica, Cymbopogon goeringii, and Eccoilpus cotulifer 4. Red wood ants seemed to build the nest by using red pine, Arundinella hirta, Miscanthus purpurascens, Themeda japonica or Cymbopogon goeringii as a fixing plant. 5. The limited point of humidity percent in habitat of red wood ants was estimated as $76\%$ during the acting period of May to September and as $72\%$ during pre-period of hibernation of October to November. 6. Soil analysis in habitating region showed higher concentration of organic matters and lower concentration of calcium and magnesium, and habitat was largely composed of silt and fine sand rather than coarse sand. 7. When the separated colony was transplanted to non-habitating red pine forest that seemed to have the similiar conditions as those of habitat, propagation and establishment of nest was possible.