• Journal of Periodontal and Implant Science
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• v.25 no.2
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• pp.372-385
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• 1995

본 연구의 목적은 비외과적 치주치료시 부가적으로 사용하기 위해 실험적으로 개발한 젤 형태의 테트라사이클린 및 테트라사이클린-구연산 혼합젤의 치근면에 대한 시간에 따른 활성도를 측정하고, 이를 용액 형태의 테트라사이클린 제제 또는 클로르헥시딘 들과 비교하는 것이다. 6명의 환자로 부터 18개의 발치된 치아를 실험대상으로 하였으며, 치아는 발치한 즉시 치석제거술과 치근활택술을 시행한 후 각 각 4개씩 4군으로 나누어 다옴과 같은 처치롤 하였다 ; 1) O.1% 클로르핵시딘 용액에 5분간 침전; 2) 50mg/ml의 테트라사이클린 용액에 5분간 침전; 3) 5% 테트라사이클린 젤에 5 분간 처리 ; 4) 테트라사이클린-구연산 혼합첼로 5 분간 처치; 5) 그리고 2개의 치아는 대조군으로서 멸균된 생리식염수에 5분간 처리하였다. 침전후 치아는 1ml의 tris-buffered saline이 담긴 용기에 옮겨 24시간 간격으로 탈착된 TBS용액올 교체하면서 실온에서 22일간 보관하였다. Porphyromonas gingivalis를 indicator organism으로 하여 microtiter assay를 이용하여 홉광도를 측정 함으로써 제거 된 용액 의 항 미생물 활성올 측정하였다. 1. 50mg/ml 의 테트라사이클린 수용액에 침전되었던 군은 생리식염수로 처리한 군에 비하여 17 일간 클로르헥시딘으로 처리한 군에 비하여는 16일간 항미생물 활성에 있어서 유의성 있는 차이를 보였다. 2. 테트라사이클린 젤과 테트라사이클린-구연산 혼합첼로 처리한 군은 대조군에 비하여 각 각 4일과 3일 까지 활성올 보였다. 3. 0.1% 클로르핵시딘 용액으로 처리한 군은 생리식염수로 처치한 군에 비하여 24시간 밖에 활성을 나타내지 못했다. 4. 전반척으로 테트라사이클린-구연산 혼합첼로 처리한 군에 비하여 테트라사이클린 첼로 처리한 군의 활성이 높았으나 유의성 있는 차이롤 보이지는 않았다.

• 대한치주과학회:학술대회논문집
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• 1994.12a
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• pp.51-51
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• 1994

• Journal of Periodontal and Implant Science
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• v.24 no.3
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• pp.581-596
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• 1994

This in vitro study was undertaken to obtain optimal tetracycline concentration that aids proliferation and spreading of human periodontal ligament cells, for clinical application in root surfaces of periodontally diseased teeth. Periodontal ligament cells used in this study were obtained from explants of periodontal ligament of 1st premolar teeth which were extracted for the purpose of orthodontic treatment. The cells were cultured in Dulbecco's Modified Eagle Medium(DMEM) supplemented with 100 U/ml penicillin, $100\;{\mu}g/ml$ streptomycin and 10% FBS at $37^{\circ}C$, 100% humidity, 5% $CO_2-95%$ air. Cells were used between the third to 4th passage. After root planing of periodontally extracted teeth, the root slabs were cut with carborundum disk. In the cell proliferation experiment, experimental groups were root planing only group, immersed groups in 25, 50, 75, 100, 150mg/ml aqueous solution of Tetracycline HCl followed by a vigorous rinse in PBS. Human PDL cells at concentration of $1{\times}10^5\;cells/ml$ were seeded in each culture well which contained root slabs and incubated for 6 hours. Then, all of the root slabs were moved into new 24 culture well and incubated 24, 48 and 72 hours. The cell counting was done by inverted phase contrast microscope after trypsinization. The following results were obtained. The cell number was increased in order root planing only group, 25, 150, 50, 75, 100mg/ml of Tetracycline HCl treated group in 24, 48 and 72 hours. The maximal cell number was obtained when the root slabs were immersed in solution with 100mg/ml of Tetracycline HCl. There were statistically significant between the root planing only group and 75, 100 mg/ml of Tetracycline HCl treated group in 24 hours, between the root planing only group and 100mg/ml of Tetracycline HCl treated group in 48 hours, between the root planing only group and 50, 75, 100mg/ml of Tetracycline HCl treated group, between 25 and 100mg/ml of Tetracycline HCl treated group in 72 hours(p<0.05). In the cell spreading experiment, after 30 minutes of incubated, in the root planing only group, the cells were generally round in shape. The cell surface was mostly covered with blebs. The cells started to attach to root surface by cytoplasmic extension in 50, 100mg/ml of Tetracycline HCl treated groups, more numerous cells attached to root surface than root planing only group. Many orifices of dentinal tubule were exposed, cells showed radially spreaded cytoplasm and unspreaded central region of the cell was covered with blebs. After 6 hours of incubation, in the root planing only group, cells showed radially spreaded cytoplasm and were attached flat appearance. In 50, 100mg/ml of Tetracycline HCl treated groups, cellular margin was concaved and cytoplasm showed elongated appearance with polarity. After 24 hours of incubation, in the root planing group, cells showed characteristic polarity. In 50, 100mg/ml of Tetracycline HCl treated groups, cells showed more elongated and spindle - like appearance.

• Journal of Periodontal and Implant Science
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• v.25 no.3
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• pp.587-602
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• 1995

The purpose of this study was to compare the effects of citric acid and tetracycline HCI application to the root surfaces of periodontally diseased teeth on the proliferation and spreading of human periodontal ligament cells. The roots were prepared so that the comparison could be made among root planed, citric acid treated and tetracycline HCI treated surfaces. In the cell proliferation experiment, human periodontal ligament cells at a concentration of $1{\times}10^5$ cells/ml were seeded in each culture well with specimens and incubated for 6 hours. Then, the specimens were transferred to a fresh culture well and incubated for 24, 48, 72 hours respectively. The cell counting was done after trypsinization. In the cell spreading experiment, $1{\times}10^4$ cells/ml were seeded in each culture well and incubated for 30min, 6 hours and 24 hours at 37.5$^{\circ}C$ in a $CO_2$ incubator. Then, all specimens were fixed with phosphate buffered glutaraldehydes, postfixed with phosphate buffered osmium tetraoxide, stained with phosphate buffered tannic acid, dehydrated in ethanol, dried at a critical point, coated with gold and examined under a scanning electron microscope. The results were as follows:In the cell proliferation experiments, the number of attached cells increased more in the tetracycline treated group than in the other groups. In the initial attachment, the appearance of the tetracycline treated the groups was slightly more spread out than in the other groups. After 6 hours of incubation, it was observed in most of the cells that cell morphologic alteration went from ovoid shapes sto spindle shapes. After 24 hours of incubation, the cells of all groups had a fusiform appearance and were connected to each other by numerous cytoplasmic processes. The tetracycline and citric acid treated groups had a similar spreading appearance of periodontal ligament cells, but the tetracycline treated group was more effective in the cell proliferation than the citric acid group.

• Applied Chemistry for Engineering
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• v.35 no.1
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• pp.8-15
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• 2024

In this study, nitrogen plasma treatment was performed in 5, 10, and 15 minutes to improve the tetracycline adsorption performance of activated carbon. All nitrogen plasma-treated activated carbons showed improved tetracycline adsorption compared to untreated activated carbons. The nitrogen functional groups in activated carbon lead to chemisorption with tetracycline via π-π interactions and hydrogen bonding. In particular, in the nitrogen plasma treatment at 80 W and 50 kHz, the activated carbon treated for 10 minutes had the best adsorption performance. At this time, the nitrogen content on the surface of the activated carbon was 2.03% and the specific surface area increased to 1,483 m²/g. As a result, nitrogen plasma treatment of activated carbon improved its physical and chemical adsorption capabilities. In addition, since the adsorption experimental results were in good agreement with the Langmuir isotherm and pseudo-second order model, it was determined that the adsorption of tetracycline on the nitrogen plasma-treated activated carbon was dominated by chemical adsorption through a monolayer. As a result, nitrogen plasma-treated activated carbon can be used as an adsorbent to efficiently remove tetracycline from water due to the synergistic effect of physical adsorption and proactive chemical adsorption.

• Korean Journal of Organic Agriculture
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• v.32 no.2
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• pp.233-245
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• 2024

In a field study, we investigated the consequences associated with the irrigation of red pepper with water contaminated by three types of veterinary antibiotics (amoxicillin, oxytetracycline, and chlortetracycline) on the soil residue, the absorption-translocation, and crop yields. As a result of evaluating the residue of veterinary antibiotics in pepper cultivation soil treated with veterinary antibiotics a total of 7 times from June 7 to August 26, 2023, the three antibiotics were not detected at the background level, but in the group treated with 10-fold and 50-fold of the background level was detected at a level of 2-10 ㎍/kg. For the leaf (stem) and fruit of red pepper, all three types of veterinary antibiotics were found to be below the detection limit at all treatments. However, trace levels were detected in red pepper roots. If veterinary antibiotics are unintentionally introduced into agricultural soil in the short term, the soil environment and crop safety will not be a problem. However, long-term introduction of veterinary antibiotics in agricultural soil may have negative effects by affecting soil microbial diversity and antibiotic resistance problems, so continuous monitoring and risk assessment are deemed necessary.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.10a
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• pp.102-103
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• 2002

옥시테트라싸이클린(OTC)은 테트라사이클린계 항균물질로 비브리오병, 지느러미 붉음병, 인린 병, 아가미 썩음병 등과 같은 수산동물 질병 치료에 많이 사용되고 있는 항균제이다. 어류의 질병을 치료하기 위해 투여된 항생제에 대하여 어체내의 잔류농도에 대한 과학적 규명없이 관습적이고 반복적으로 사용되고 있어 과잉투여에 의한 경제적 손실, 과소투여에 의한 내성균의 발현, 양식 어류 체내의 항생물질 잔류 농도등 항생제 사용 문제는 양식산업뿐 아니라 수산가공 입장에서도 매우 중요한 현안으로 대두되고 있다. (중략)

• Journal of Periodontal and Implant Science
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• v.24 no.3
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• pp.443-457
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• 1994

본 연구의 목적은 비외과적 치주치료시에 부가적으로 사용하기 위해 개발한 테트라싸이클린 젤의 효과를 임상지수, 치은 열구액양 및 치은열구액내 효소활성도 측정을 통해 평가함에 있다. 치주낭 깊이가 4mm 이상 6mm이하인 구치가 편악당 적어도 2개 이상 존재하는 중등도 치주염에 이환된 환자 23명을 선택하여, 한 편악에서는 치근활택술 만을 시행하였고, 다른 한 편악에는 치근활택술 후 테트라싸이클린 젤을 치은연하로 주입한 뒤 치근면 처리하였다. 사용된 임상검사 지수는 치태지수, 치은지수, 치주낭 깊이, 치은부착 상실도 및 탐침시 출혈로, 각각 치료시작 전과 치료 후 4, 12주에 검사하였으며, 치은열구액내 효소활성도 검사를 위해 치은열구액을 치료시작 전과 치료 후 1, 2, 4, 12주에 각각 채취하고 치은열구액 양을 조사한 뒤 Lactate dehydrogenase(LDH)와 Beta-glucuronidase(BG)의 활성도를 측정하였고, 효소활성도는 LDH는 340nm에서, BG는 550nm에서 각각 흡광도를 조사 분석하여 다음과 같은 결과를 얻었다. 1. 임상지수를 측정 비교한 결과 치근활택술 만으로 치료한 군과 치근활택술 후 테트라싸이클린 젤을 병용한 군 모두에서 임상적으로 치은건강의 현저한 향상을 관찰할 수 있었으나, 통계상 두 군간의 유의성있는 차이는 발견되지 않았다. 2. 치은열구액 양은 치근활택술 후 테트라싸이클린 젤을 병용한 군이 치근활택술만 시행한 군보다 치료 후 2주와 4주에 유의성 있게 감소하였다. 3. LDH와 BG 효소 활성도는 두 군 모두 현저히 감소하였고, 비록 통계학적인 유의성은 관찰할 수 없었으나 치근활택술 후 테트라싸이클린 젤을 병용한 군에서 감소량이 더 크게 나타났다.

• Journal of Food Hygiene and Safety
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• v.22 no.4
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• pp.370-374
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• 2007

An analytical method for the simultaneous determination of four tetracycline (oxytetracycline, tetracycline, chlortetracycline, doxycycline) in egg samples was developed and validated using liquid chromatography with ultraviolet detection. Egg samples were extracted by the liquid-liquid extraction based on acetonitrile. The chromatographic separation was achieved on a reverse phase C8 column with gradient elution using a mobile phase of 20 mM oxalic acid (pH 1.5)/acetonitrile. The procedure was validated according to the Food Drugs Administration guideline determining accuracy, precision, and limit of detection. Mean recovery of tetracyclines from spiked egg samples (50, 100, 200, 400, and $800{\mu}g/kg$) were 78.8-109.3%. Linearity in concentration range of $50-800{\mu}g/kg$ was obtained with the correlation coefficient $(r^2)$ of 0.994-0.999. The intra- and inter-day precision (relative standard deviation; RSD) was between 0.3-12.8 and 0.2-11.7%, respectively. Limit of detection (LOD) and limit of quantification (LOQ) for the investigated tetracyclines were 30 and $50{\mu}g/kg$ depending on egg samples, respectively. This method was reliable, sensitive, economical and suitable for routine monitoring of tetracycline residues in dairy egg.

• 월간낙농육우
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• s.98
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• pp.83-85
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• 1990