• Ocean and Polar Research
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• v.33 no.2
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• pp.127-133
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• 2011

The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide ($H_2O_2$); strong correlation between $H_2O_2$ concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1, 10, 50, 100 ${\mu}g/L$) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.

• Environmental Analysis Health and Toxicology
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• v.25 no.1
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• pp.19-26
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• 2010

Discharged materials from the point or non-point source are released into the sea, and as the results, marine environment is directly affected. We must estimate the impacts of contaminants to marine pollution rapidly and accurately. Therefore, it is needed on early warning system for appreciating marine environmental impacts, and required a bioassay to evaluate abnormal changes. A bioassay test was developed to examine the effects of heavy metal contaminants on the early life stages of the marine annimals. We have studied the effects of metals on early development of a sea urchin species, Strongylocentrotus intermedius. S. intermedius embryos were tested with six metals (Cu, Ag, Zn, Cd, Cr, Ni) and showed the highest sensitivity to Cu as well as the lowest sensitivity to Cd. The order of biological impact for metals was Cu>Ag>Ni>Zn>Cr>Cd. In accordance with the results, sea urchins embryos can provide biological criteria for seawater quality assessment. The sensitivity of developmental bioassay whith S. intermedius is at intermediate level among marine organisms commonly used in aquatic bioassays. And this sea urchin can be routinely employed as a test organism for ecotoxicity assays.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.173-176
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• 2012

본 연구는 빈산소에 의한 둥근 성게 (Strongylocentrotue nudus) 수정란의 수정률 및 발생률에 관한 연구이다. 대조군 (normoxia)과 실험군 (hypoxia) 으로 나누어 수정률과 발생률의 변화를 관찰 하였으며 또한 gonad 세포의 유전자 발현의 차이를 봄으로써 스트레스 관련 유전자와 항산화 관련 유전자의 변화를 확인 할 수 있었다. 결과적으로 수정률에서는 큰 차이를 확인 할 수 없었던데 반해 발생률에 있어서 빈산소의 경우 전혀 발생이 진행되지 않는 것을 확인 할 수 있었으며 또한 빈산소에 노출된 gonad 세포의 경우도 스트레스 또는 항산화 유전자가 많이 발현 되는 것을 관찰 할 수 있었다. 앞의 실험을 토대로 빈산소 환경에서 유전자 발현량의 차이를 더욱더 수행함으로써 빈산소 상태에 따른 죽음의 바다의 증가 얼마나 위험한 것인지 더욱더 관찰 할 수 있을 것이다.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.274-277
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• 2012

지구온난화의 폐해 중 하나인 해파리의 대량증식은 환경 생태계에 좋지 않은 영향을 미치고 있고, 또한 과도한 개체수는 원할한 처리가 곤란해 많은 문제점을 초래하고 있는 실정이다. 이러한 해파리의 생산적이고 효율적인 개선방안으로 단순 가공식품 뿐만 아니라 해파리의 추출을 통해 산업적으로 활용가능한 기능성 화장품원료로의 응용가능성도 고려해 볼 수 있을 것이다. 따라서 본 연구는 보름달물 해파리 유래 추출물의 기능성 화장품 원료로서의 안전성 및 유효성을 규명하였다. 인간피부섬유아세포에서의 세포독성, 주름 개선 효과와 관련된 procollagen 발현양의 증가 및 노화로 증가되는 SA-${\beta}$-Gal 효소의 활성을 염색을 통하여 확인하였고, 항노화 화장품 신소재로서의 사업화 가능성을 확인 할 수 있었다.

• Korean Journal of Applied Biomechanics
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• v.24 no.3
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• pp.277-285
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• 2014

Nakcyguri is one of the offensive weapons most often used in Taekkyon games, The purpose of this study was to investigate kinetic factors on two types of Nakcyguri, one of which uses hip bending and the other uses little it. 12 taekkyoners (males) who are the students of Y University participated in this study. They have been practicing on Taekkyon for five years or more. Positions of CoM, the elapsed time of each phase, ROM of joint and segment angles, ground reaction forces, joint moments of supporting leg were analyzed for this study. The results were as follows; in Nakcyguri with hip bending than without hip bending, anterior/posterior ROM of pelvis, head and swing foot were larger by flexing-extending more, and both vertical and posterior components of GRF were larger during phase 4. It was considered that hip bending produced posterior momentum as well as vertical momentum of trunk during Nakcyguri. In conclusion hip bending was essential and so useful to the successful attack.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.11a
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• pp.61-71
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• 2002

광양만내 해양바이러스양은 2.0$\times$$10^{8}$ particles/ml로 매우 풍부했다. 각 계절별 바이러스의 밀도는 여름에 최대 9.0$\times$$10^{8}$ particles/ml에서 겨울에는 최소값인 0.7$\times$$10^{8}$ particles/ml을 기록했다. 광양만 내의 바이러스, 박테리아, 식물플랑크톤 생물량의 공간적 분포는 open ocean system에 해당하는 정점 28, 38, 42, 46, 51에서 보다 closed estuarine system에 해당하는 정점 2, 5, 10 ,12, 16, 20에서 많은 것으로 나타났다. 그리고, 다른 closed estuarine system에 해당하는 정점 22, 26, 32, 34는 높은 바이러스 밀도를 보였지만 상대적으로 open ocean system에 비해서 낮은 박테리아와 식물플랑크톤의 생물량을 나타냈다. 몇몇 정점의 수심 깊이에서는 박테리아의 밀도가 바이러스의 밀도를 100배정도 초과했다. 해양 바이러스와 그들의 host system의 계절별 밀도는 역동적으로 번화했으며, 그들의 계절별 변화는 밀집한 상호연관성을 가졌다. 여름에 바이러스와 박레리아의 밀도는 증가된 반면 식물플랑크톤의 엽록소-a 농도는 평균값을 유지하였다. 겨울에는 바이러스와 박테리아의 밀도가 급속하게 줄어들었고, 마찬가지로 엽록소-a 농도 역시 감소하다가, 곧 다시 증가했다. 바이러스의 밀도는 2001년 8월에 최고점에 도달했으며, 박테리아의 밀도는 2001년 8월과 2002년 6월에 최고값을 가졌다. 반면에 엽록소-a의 농도는 2002년 4월에 최대치에 도달했다. 전체 host와 바이러스 밀도로 볼 때, 그들의 pool은 바이러스에 의한 mortality에 의해서 steady-state 상태로 유지되고, 바이러스의 밀도 또한 steady-state를 유지하는 것으로 보였다. 이러한 시도는 우리 나라 광양만 내에 존재하는 해양 바이러스의 생태적 분포 연구를 다루는 첫 번째 실험으로 사료된다.

• Korean Journal of Applied Biomechanics
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• v.23 no.2
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• pp.131-139
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• 2013

Naejirgi is one of the fastest, most forceful and most often being used kicks in Taekkyon games, The purpose of this study was to investigate kinetic factors on two types of Naejirgi kick, one of which uses knee bending of supporting leg and the other uses little it. 12 taekkyoners (11 males and one female) who are the students of Y University participated in this study. They have been practicing on Taekkyon for five years or more. Positions of CoM, the elapsed time of each phase, vertical ground reaction forces, joint moments and impulses of supporting leg were analyzed for this study. The results were as follows; in Naejirgi with knee bending of supporting leg than without knee bending of supporting leg, the vertical motion range of whole body CoM was larger during phase 2 and 3, the elapsed time of phase 4 were longer, players stayed longer in the nearest location to opponent, during phase 4 the vertical ground reaction forces of supporting foot were larger, and joint extension moments and angular impulses of supporting leg (especially knee) were larger. In conclusion supporting knee bending is not a useful strategy for Naejirgi, because players stay longer in the nearest position to opponent and consumed more muscle force and energy for producing the vertical momentum which is unnecessary for pushing down the opponent.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.7
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• pp.3582-3588
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• 2013

Expressed sequence tag (EST) library was constructed from A. tamarense. Base sequences of EST clones were analyzed and saxitoxin biosynthesis-related genes were cloned. Sequences of 827 clones were analyzed and 564 EST were functionally clustered using Blast searches against GenBank. Main genes in the EST had functions on cellular organization, cell metabolism, energy, cell cycle and DNA processing, cellular transport and transport, cell rescue, defense, death and aging, and transcription. Moreover, expression of S-adenosylmethionine synthetase and H2A histone family genes were increased in the toxic A. tamarense. These results show that two genes could be a good biomarkers for the detection of saxitoxin biosynthesis in the A. tamarense.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.10
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• pp.4910-4916
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• 2012

Changes of low concentrated nitrogen-phosphate removal efficiency were investigated in 4 strains of marine bacteria applied to ceramic media. Marine bacteria were isolated and identified from Gwangyang bay. Growth rates and removal efficiencies of $NH_3$-N of 4 strains of marine bacteria applied to ceramic media were increased approximately 3 fold and over 30% than control group, respectively. A. hydrophila and P. diminuta had highest ${NO_3}^-$-N and phosphate removal efficiencies, respectively. This results showed that ceramic media is very nice material for improvement of nitrogen-phosphate removal efficiency and isolated marine bacteria may be useful to control nitrogen-phosphate at low concentration in field.

• The Journal of the Korea Contents Association
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• v.15 no.7
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• pp.57-74
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• 2015

This paper will concentrates on martialarts acting system. I will sugest a kind of actor's training system for the safe method of action acting. In this study I solve the following problems. First, what is main factors to interfere with performer to act and how do they get rid of factors. Second, what is very economic and effective challenge to develope the new acting system for action acting performers. For this discussion I studied four parts. The first, I gives purpose and direction of the safe for martialarts actor. The second, I traces the development of contemporary idea of acting such as Stanislavsky and Meyerhold. The third I introduces Korean martialarts training system. The last I conclude which refer to effective action acting by result of training 'Korean traditional martialarts acting system'.