Low Concentrated Nitrogen-Phosphate Removal of 4 Strains of Marine Bacteria Applied to Ceramic Media

세라믹 담체에 적용된 해양박테리아 4종의 저농도 질소-인 제거

  • Received : 2012.07.31
  • Accepted : 2012.10.11
  • Published : 2012.10.31


Changes of low concentrated nitrogen-phosphate removal efficiency were investigated in 4 strains of marine bacteria applied to ceramic media. Marine bacteria were isolated and identified from Gwangyang bay. Growth rates and removal efficiencies of $NH_3$-N of 4 strains of marine bacteria applied to ceramic media were increased approximately 3 fold and over 30% than control group, respectively. A. hydrophila and P. diminuta had highest ${NO_3}^-$-N and phosphate removal efficiencies, respectively. This results showed that ceramic media is very nice material for improvement of nitrogen-phosphate removal efficiency and isolated marine bacteria may be useful to control nitrogen-phosphate at low concentration in field.

세라믹담체에 적용한 4종의 해양박테리아 (Aeromonas hydrophila, Chryseomonas indologenes, Pseudomonas diminuta, Vibrio parahaemolyticus)의 저농도 질소 인 제거 효율의 변화를 분석하였다. 해양박테리아는 광양만에서 분리 동정하였다. 담체에 적용한 4종의 해양박테리아 모두 대조군에 보다 약 3배 정도의 성장률이 증가하였으며, 암모니아서 질소 제거효율도 30% 이상 증가하였다. 질산성 질소의 제거 효율은 A. hydrophila 균주가 가장 높았으며, 인의 제거는 P. diminuta 균주가 가장 높은 것으로 나타났다. 본 연구의 결과는 세라믹담체는 질소-인 제거 효율 증진에 좋은 재료이며, 분리된 해양박테리아는 현장의 저농도 질소-인 조절에 유용할 수 있음을 보여준다.



Supported by : 해양과학기술원


  1. J. W. Fleeger, K. R. Carman, and R. M. Nisbet, "Indirect effects of contaminants in aquatic ecosystems", Sci. Total. Environ. 317, 207-233, 2003.
  2. L. J, Guillette and E. A. Guillette, "Environmental contaminants and reproductive abnormalities in wildlife: implications for public health?" Toxicol. Ind. Health 12, 537-550, 1996.
  3. K. Schiff, S. Bay, "Impacts of stormwater discharges on the nearshore benthic environment of Santa Monica Bay." Mar. Environ. Res. 56, 225-243, 2003
  4. M.-S. Lee, J.-H. Park, "Isolation of ammonia oxdizing bacteria and their characteristics". J. Korean Fish. Soci., 31, 760-766, 1998.
  5. J.-Y. Jung, S.-W. Lee, K.-H. Kim, J.-H. Lim, J.-K. Lee, "Removal of N,P in seawater by zeolite" Theo. App. Chem. Eng. 8(2), 3773-3776. 2002
  6. Y-S. Park and K-H Ahn. "Preparation of ceramic support carrier and investigation of performance." J. Kor. Society of Environ. Engineers. 23(3), 507-516, 2001.
  7. B. H. Jun, "Nitrogen removal in fluidized bed and hybrid reactor using porous media" J. Kor. Soc. Environ. Eng. 27(5), 542-548, 2005.
  8. G. M. Kim, W. Hur, H. J. Baek, "Treatment of acid mine drainage using immobilized beads carrying sulfate reducing bacteria" Econ. Environ. Geol. 41(1), 57-62, 2008.
  9. S.-J. Kim and S.-S. Lee, "The development of treatment system for removing the low concentrated nitrogen and phosphorus using phototrophic bacteria and media" Kor. J. Microbiol. 46(1), 27-32, 2010.
  10. Young Seek park, Ki Woo Koo, Young Soo Na, Seung Koo Song, "Physical Characteristics of Support Media for Microbial Immobilization." J. Environ. Sci. 7(3), 269-274, 1998.
  11. Sung Youl Park, Do Han Kim, Young Seek Park, Seung Koo Song, "Characteristics of Ceramic and Polymer Support Carrier affecting the Microbial Attachment under Anaerobic Condition." Kor. Environ. Eng. 23(6), 951-959, 2001.
  12. G. Lee, S. H. Moh, Y. Chung, S. J. Kim, Y. J. Kim, S-S Lee, T.-K. Lee, "Isolation and identification of marine bacteria with high removal efficiencies for nitrogen-phosphate in Gwangyang bay" J. Korea Acad-Indus. Soci. 13(7),
  13. M. Madigan, S. S. Cox, and R. A. Stegeman, "Nitrogen fixation and nitrogenase activities in members of the family Rhodospirillaceae." J. Bacteriol. 157, 73-78, 1984.
  14. K. L. Casciotti, and B. B. Ward, Dissimilatory nitrite reductase genes from autoteophic ammoniaoxidizing bacteria." Appl. Environ. Microbiol. 67, 2213-2221, 2001.
  15. S.-S. Lee, H.-J. Joo, S. Lee, M. Chang, T.-K. Lee, H. Shim, E. Shin, "Development of advanced wastewater treatment system using phototrophic purple non-sulfur bacteria." Kor. J. Microbiol. Biotechnol. 30, 189-197, 2002.
  16. S. S. Lee, "The Advanced Biological Treatment System Using Photosynthetic Bacteria." News & Information for Chemical Engineers, 22, 20-22, 2004
  17. I. S. Kulaev, A. Sha, and S. E. Mansurova, "Poluphosphates of phototrophic bacteria Rhodospirillium rubrum under different cultivation conditions. Biochemia, 36, 656, 1974.