• Journal of Life Science
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• v.18 no.2
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• pp.264-272
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• 2008

The lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes in tissues from Acanthogobius hasta were characterized by biochemical, immunochemical and kinetic methods. The activities of LDH in skeletal muscle and eye tissues were 65.30 and 53.25 units, but LDH activities in heart and liver tissues were very low. LDH/CS (EC 4.1.3.7, citrate synthase) in skeletal muscle was the highest as 22.29. Specific activities of LDH in brain, eye and skeletal muscle were 56.45, 38.04 and 11.0 units/mg, respectively. The LDH isozymes in tissues were separated by polyacrylamide gel electrophoresis after immunoprecipitation with antiserum against $A_4,\;B_4$ eye-specific $C_4$ and liver-specific $C_4$. LDH $AC_4$ isozymes were detected predominantly in skeletal muscle, brain and eye tissues, and $B_4$ isozyme was detected in heart. Anodal eye-specific $C_4$ and cathodal liver-specific $C_4$ were coexpressed in A. hasta. The eye-specific $C_4$ isozyme showed higher activity in eye tissue, but liver-specific $C_4$ isozyme showed lower activity in liver. As a result, one part of molecular structures in $A_4\;and\;C_4,\;A_4\;and\;B_4$, and eye-specific $C_4$ and liver-specific $C_4$ were similar, but in $B_4\;and\;C_4$ were different with each other. Therefore the subunit A may be conservative in evolution, and the evolution of subunit B seems to be faster than that of subunit A. The LDH $A_4$ isozyme of skeletal muscle was purified in the fraction from elution with NAD+ containing buffer of affinity chromatography and eye-specific $C_4$ isozyme was eluted right after $A_4$, so the structure of eye-specific $C_4$ isozyme is similar to $A_4$. And LDH activity remained 35.22-43.47% as a result of the inhibition by pyruvate, the Michaelis-Menten constant values for pyruvate was 0.080-0.098 mM, and Vmax were 153.85 units, 35.09 units in skeletal muscle and eye, respectively. Also the $B_4$ isozyme was the thermo-stablest and $C_4$ was stabler than $A_4$ isozyme. The optimum pH of LDH was 6.5. The results mentioned above indicate that isozymes in tissues showed the properties between LDH $A_4\;and\;B_4$ isozyme as A. hasta was adapted to hypoxic conditions. Also LDH seems to function more effectively under anaerobic condition because LDH in skeletal muscle and eye tissues have high affinity for pyruvate.

• Journal of Soil and Groundwater Environment
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• v.10 no.6
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• pp.68-74
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• 2005

This study was carried out to evaluate the feasibility of field-scale sequential soil washing process for remediation on Kyongsangnamdo D mine soils which was heavily contaminated by arsonic. Arsenic concentration of untreated soils was $321\pm32mg/kg$. By applying the basic operating condition which was proposed from several pilot-scale experiments, arsenic concentration of treated soils was reduced 2.04 mg/kg ($99\%$ removal efficiency). We optimized the basic operating condition (mainly on washing solution concentration, cut-off size, and mixing ratio) to improve efficiently and economically the field-scale sequential soil washing process. The resulting optimized conditions were that solution concentration is 0.2M HCl, 1.0M HCl, 1.0M NaOH, that the cut-off size is 0.15mm (seive $\sharp$100), and that the mixing ratio is 1 3. Also, the optimized pH value for soil washing effluent treatment was 6 (33 ppb), in which the precipitation disruption caused by supersaturation of the floe did not occur. Results of TCLP tests showed that arsenic concentration from the washed gravels was 1.043 mg/L, that from soils ND (not detected), and that from filter cakes 0.066 mg/L. Also, the water content as a percentage of dewatered sludges was low $(48\%)$ and so the dewatered sludges can be disposed by landfilling. Through these results, we can concluded that tile field-scale sequential soil washing process developed in this study is adopted for remediation of arsenic-contaminated soils.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.10
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• pp.583-589
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• 2015

This study was performed to evaluate the feasibility of anaerobic pretreatment for the leachate solubilized from thermal hydrolysis of sewage sludge cake. Overall process for the treatment of sludge cake consists of thermal hydrolysis, crystallization of magnesium, ammonium, and phosphate (MAP) for the leachate and anaerobic digestion of supernatant from MAP crystallization. The experimental evidence showed that the optimum ratio of Mg : P for the struvite crystallization of leachate solubilized from thermal hydrolysis of sludge cake was 1.5 to 1.0 as weight basis at the pH of 9.5. With this operational condition, the removal efficiencies of ammonia nitrogen and phosphorous achieved 50% and 97%, respectively. The mesophilic batch test showed that the ultimate biodegradability of the supernatant from MAP crystallization reached 63% at S/I ratio of 0.5. The readily biodegradable fraction of 90% ($S_1$) of the MAP supernatant BVS (Biodegradable Volatile Solids, $S_0$) degraded with $k_1$ of $0.207day^{-1}$ for the initial 17 days where as the rest slowly biodegradable fraction ($S_2$) of 10% of BVS degraded with $k_2$ of $0.02day^{-1}$ for the rest of the operational period. Semi-Continuously Fed and Mixed Reactor (SCFMR) was chosen as one of the best candidates to treat the MAP supernatant because of its total solids content over 6%. Maximum average biogas production rates reached 0.45 v/v-d and TVS removal efficiency of 37~41% was achieved at an hydraulic retention time (HRT) of 20 days and its corresponding organic loading rate (OLR) of 1.43 g VS/L-d.

• Journal of the Korean Society for Environmental Technology
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• v.19 no.6
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• pp.556-562
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• 2018

Due to the enforcement of effluent water regulation the advance sewage treatment system is needed to retrofit and remodelling. In this case the most important issue is the effluent concentrations of TP and there are a lot of system to reduce its concentration. But biological treatment processes have many restrictions to enhance the removal efficiency of TP. In this study the efficient ECO operating condition to improve and retrofit biological sewage wastewater treatment system is as follow; 1) The treatment efficiency of BOD, TN and TP at the current density of $15mA/cm^2$ was higher than the treatment efficiency at $5mA/cm^2$ in the electrodes arranged with Al-SUS(Stainless Steel) regardless of the reaction time, The TP concentration was 0.1 mg/L or less. Especially, when the reaction time was maintained at 10 min, the TP concentration was 0.06 mg/L or less irrespective of the current density. 2) The change of TP concentration is not influenced by the change of current density and rather the concentration of treated water changes according to the reaction time. In the case of electro coagulation reaction, a few seconds to several minutes are required. However, the reaction time of electro coagulation and oxidation was studied to be more than 10 minutes. 3) As a result, it has been studied that the economical current density of the electro coagulation oxidation process for TN and TP treatment of domestic wastewater is $15mA/cm^2$ or less and the reaction time is 10 minutes.

• Clean Technology
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• v.11 no.2
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• pp.57-67
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• 2005

Epoxy resins are produced by the dehydro condensated reaction with ECH (Epichlorohydrin) and BPA(Bisphenol-A) as raw reactants under sodium hydroxide(NaOH) as a catalyst, and from this reaction, salted water named of brine, which contains ECH derivatives in condition of emulsion as likely as glycidol and polymer resins, is produced as an undesired side product. This brine water is alkaline wastewater and causes process fouling problems by plugging and chemically depositing polymer particles on the surface of inner wall of reactors and pipes, and decreases the biodegradable efficiency in the wastewater process. In this study, the optimization of coagulation and sediment reactions, using inorganic and organic polymer coagulants, were performed to remove the causes occurring the process fouling phenomena. And also, based on this study, the methodologies applicable to the commercial processes including economical analysis were presented.

• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.1
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• pp.52-62
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• 2015

Coagulating effects of eco-friendly coagulant materials such as zeolite, kaolinite, loess and chitosan upon sewage sludge were evaluated. And coagulating effects of the mixtures of those materials and polymer (polyacrylamide) were also investigated. Coagulating efficiency of loess was higher than that of kaolinite and zeolite at the treatment levels of 2,500~50,000mg/L. Coagulating efficiencies of zeolite, kaolinite and loess at the treatment level of 20,000mg/L were lower than that of 1,000 mg/L of polymer, which meant that single application of those materials in real plant were not feasible. At the treatment level of 1,000 mg/L, coagulating efficiency of Chitosan was much lower than that of polymer, which also meant that single use of chitosan in real plant could be unrealistic because of its high price. However, the application of the mixture of 'polymer 80mg/L + kaolinite or loess 500 mg/L + chitosn 10mg/L' or the mixture of 'polymer 80mg/L + kaolinite or loess 500mg/L' was promising way for coagulating sewage sludge.

• Applied Chemistry for Engineering
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• v.8 no.6
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• pp.1029-1033
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• 1997

Fundamental research of non-isothermic analysis of reaction rate has been carried out for the heat storage system using the thermal decomposition of $Na_2B_4O_7{\cdot}10H_2O$. It was found that the equilibrium temperature of the thermal decomposition reaction was lowered less than 373K in $Na_2B_4O_7{\cdot}10H_2O/Na_2B_4O_7{\cdot}5H_2O$ system, but the heat efficiency was unchanged. The initiation temperature of the reaction was varied from low to high temperature region with heating rate. The reaction order of the dehydration reaction by the thermal decomposition was appeared to be 0.67 by non-isothermic analysis, thereby $Na_2B_4O_7{\cdot}10H_2O$ may be used as a hemical heatstorage material.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.84-89
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• 2003

The purpose of this study was to evaluate and improve the pre-treatment facility for foodwaste and sewage sludge mixture treatment. The process of foodwaste pre-treatment consists of storage, classification with crushing, and thickening. The effluent of sewage treatment facility was used as the diluting and washing water. The panicle size of foodwaste after pre-treatment was almost under 2mm, the mixture of foodwastes and sewage sludge showed an advantage to the anaerobic digestion. The amount of gas production increased from 0.8ton/day ($CH_4$ : 0.5ton/day) to 3.5ton/day ($CH_4$ : 2.3ton/day) after the anaerobic digestion of the foodwastes and sewage sludge mixture. The amount of sludge cake increased from 11.2ton/day to 21.2ton/day. Therefore, the proper operation of the foodwaste pre-treatment facility was contributed to the efficient anaerobic digestion of foodwaste and sewage sludge mixture treatment.

• Clean Technology
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• v.9 no.3
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• pp.115-124
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• 2003

Bio industry had rapidly grown up to about 32% annualy mean since '90. To overcome a trade barrier related with environmental issues, which were needed to reduce and reuse pollutants generated from the manufacturing process of product to develop and apply cleaner technology. In this paper, the usefulness of membrane process were surveyed for economical and effective treatment of waste water which were discharged from the production of polymannuronic acid. Concentration efficiencies for U/F 10k, U/F 1k, and N/F were 40%, 60%, and 80% by batch operation. Concentration efficiencies for a combination process of U/F 1k and N/F were 90% by continuous operation. There were a lot of loss of product, which could improve 25% of productivity with recovering product by introducing membrane process.

• Food Science and Preservation
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• v.15 no.5
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• pp.694-698
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• 2008

Green pepper was dried using 30%, 50%, or 80% maltodextrin as dehydrating agents, and the quality of the peppers was compared with that of freeze-dried and hot air-dried samples in terms of rehydration ratio, color, and sensory evaluation. The amount of moisture lost during drying increased with increasing concentrations of maltodextrin. The rehydration ratio of maltodextrin-treated pepper was greater than those of freeze-dried or hot air-dried peppers. The color of the 30% maltodextrin-treated green pepper was similar to that of freeze-dried pepper and better than that of hot air-dried pepper. On sensory evaluation of dried green peppers, maltodextrin-treated pepper scored better than did the freeze-dried or hot air-dried samples. These results suggest that drying of green pepper using maltodextrin is very efficient, because good rehydration capacity is retained and minimal cell destruction may be achieved.