• Title/Summary/Keyword: 클론

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Cross-Language Clone Detection based on Common Token (공통 토큰에 기반한 서로 다른 언어의 유사성 검사)

  • Hong, Sung-Moon;Kim, Hyunha;Lee, Jaehyung;Park, Sungwoo;Mo, Ji-Hwan;Doh, Kyung-Goo
    • Journal of Software Assessment and Valuation
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    • v.14 no.2
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    • pp.35-44
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    • 2018
  • Tools for detecting cross-language clones usually compare abstract-syntax-tree representations of source code, which lacks scalability. In order to compare large source code to a practical level, we need a similarity checking technique that works on a token level basis. In this paper, we define common tokens that represent all tokens commonly used in programming languages of different paradigms. Each source code of different language is then transformed into the list of common tokens that are compared. Experimental results using exEyes show that our proposed method using common tokens is effective in detecting cross-language clones.

Application of Cyclone to Removal of Hot Particulate in Hot Cell (Hot Cell 내의 고방사능 분진 제거를 위한 사이클론 적용 실험)

  • Kim Gye Nam;Lee Sung Yeol;Won Hui Jun;Jung Chong Hun;Oh Won Zin
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.3 no.1
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    • pp.67-75
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    • 2005
  • The size and main ingredient of hot particulate generated during the nuclide experiment in hot cells of nuclear facilities were 0.5300 $\mu$m and UO$\_2$. A cyclone filter equipment which consists of a cyclone and Bag/HEPA filter was devised to remove hot particulate generated during the nuclide experiment in hot cells of nuclear facilities. The experimental conditions to maximize the collection efficiency of hot particulate were suggested through experiments done with the cyclone filter equipment. With the large size of simulated particulate, the collection efficiency of the particulate was high. When the size of simulated particulate was more than 5 $\mu$m, the collection efficiency of the particulate was more than $80\%$ and when the size of simulated particulate was less than 1.0 urn, the collection efficiency decreased by less than $70\%$. If the inflow velocity of simulated particulate was increased, the collection efficiency of the particulate was also increased. When the inflow velocity of simulated particulate was more than 12m/sec, the collection efficiency was higher than $70\%$, but after 17 m/sec inflow velocity, no change observed. The collection efficiency of the simulated particulate can be enhanced with the length of vortex finder inside the chamber. With the length of vortex finder, 7.2cm, the observed collection efficiency of the particulate was the maximum. Moreover, when the sub-cone was attached under the cyclone, the collection efficiency of cyclone increased $2\%$. It was found that effect by attachment of sub-cone was not serious.

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Detection of Streptococcus mutans in Saliva using Monoclonal Antibodies (단일클론항체를 사용한 타액 내 Streptococcus mutans 검출)

  • Lee, Minjeong;Lee, Daewoo;Kim, Miah;Lee, Kyungyeol;Baik, Byeongju;Yang, Yeonmi;Kim, Jaegon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.42 no.1
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    • pp.10-21
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    • 2015
  • The purpose of this study was to determine the usefulness of a detection method for Streptococcus mutans in saliva with monoclonal antibodies developed targeting Ag I/II and glucosyltransferases (gtf B, gtf C and gtf D) in Streptococcus mutans. In the three groups tested (adults, minors, and minors under orthodontic treatment), the results of the DMFT scores, the colony density (CFU/mL) in their saliva was measured using $Dentocult^{(R)}$-SM strip mutans, polymerase chain reaction was performed to test whether Streptococcus mutans and Streptococcus sobrinus were present, and Streptococcus mutans detecting tests performed in their saliva using four types of monoclonal antibody were collected. In conclusion, it was demonstrated that the Streptococcus mutants plays more important role in forming dental caries compared to Streptococcus sobrinus, and that the monoclonal antibodies against glucosyltransferases (gtf B, gtf C, gtf D) and Ag I/II of Streptococcus mutans are superior in detecting Streptococcus mutans to $Dentocult^{(R)}$-SM strip mutans or polymerase chain reaction.

Dust collection optimization of tunnel cleaning vehicle with cyclone-based prefilter (사이클론 전처리부를 지닌 터널집진차량의 집진효율 최적화)

  • Jeong, Wootae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.3
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    • pp.679-686
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    • 2018
  • A new dust cleaning vehicle is needed to remove fine and ultra-fine particulate matter in subway tunnels. Therefore, the recently developed tunnel cleaning vehicle is equipped with an efficient suction system and cyclone-based prefilter to handle ultra-fine particles. To treat various sizes of particulate matter with an underbody suction system, this paper proposes a cyclone-based prefilter in the suction system and validates the dust removal efficiency through Computational Fluid Dynamics (CFD) analysis using ANSYS FLUENT. Using the created surface and volume mesh, various particle sizes, materials, and fan flow rates, the particles were tracked in the flow with a discrete phase model. As a result, the dust cleaning vehicle at a normal operational speed of 5km/h requires at least a fan flow rate of $1500m^3/min$ and 100mm of suction inlet height from the tunnel track floor. Those suction modules and cyclone-based prefilters in the dust cleaning vehicle reduces the dust accumulation load of the electric precipitator and helps remove the accumulated fine and ultra-fine dust in the subway tunnel.

Vinblastine Determination Measured by a Sensitive ELISA Inhibition Assay (ELISA Inhibition Assay에 의한 Vinblastine의 측정)

  • Jae Wha kim;Mi Young Han;Hee Gu Lee;Eun Young Song;Tai Wha Chung;Kyung Soo Nam;In Seong Choe
    • Biomedical Science Letters
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    • v.2 no.1
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    • pp.121-126
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    • 1996
  • Specific monoclonal antibodies(mAbs) against bis-indole alkaloids related to vinblastine were established to develop a simple and specific immunoassay system for the quantitation of Vinca alkaloids. Vinca alkaloids were extracted from tissue cultured cells of periwinkle plant (Vinca rosea L.). Spleen cells from Balb/c mice immunized with vinblastine-bovine serum albumin(VBL-BSA) conjugate as immunogen were fused with myeloma cells(Sp2/0-Ag.14) in the presence of polyethylene glycol. In the preliminary experiments, 32 clones which highly reacted with VBL-BSA conjugate were selected by ELISA(Enzyme-linked immunosorbent assay).These clones were further analyzed by inhibition assay of ELISA. The results obtained with two typical monoclonal antibodies, KN-1 and KN-2, were described. KN-1 exhibited considerable reactivities with soluble dimeric bis-indole alkaloids, whereas no cross reacted with monomeric bis-indole alkaloids. However KN-2 showed cross reactivity with mono- and di-meric bis-indole alkaloids. Furthermore, KN-1 was applied to the immunoassay system for determining the VBL amounts of in vitro cultured cell extracts. This assay system could detect dimeric vinca alkaloid as low as 0.05 nM.

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Immunoblotting Assay for Glyphosate-tolerant Genetically Modified Soybean in Soybean Products (식품 중 제초제내성 유전자재조합 콩의 immunoblotting 분석)

  • Son, Dae-Yeul;Moon, Jung-Hee;Ahn, Kang-Mo;Shon, Dong-Hwa;Lee, Kwang-Shin;Sim, Hee-Young;Han, Young-Shin;Lee, Sang-Il
    • Korean Journal of Food Science and Technology
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    • v.36 no.3
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    • pp.369-374
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    • 2004
  • Genetically modified (GM) soybean Roundup Ready carries Agrobacterium sp. CP4 gene, which expresses 5-enolpyruvylshikimate-3-phosphate synthase (CP4EPSPS). CP4EPSPS in GM soybeans and soybean curds was screened using CP4EPSPS-specific polyclonal and monoclonal antibodies (pab and mab, respectively) by immunoblotting. Isolated recombinant CP4EPSPS was detected at detection limits of $0.006\;and\;0.0006{\mu}g$, whereas those of CP4EPSPS expressed in GM soybean were $0.001\;and\;0.0001{\mu}g$g, using mab and pab, respectively. From nine screened soybean curds, two had positive results with pab Immunoblotting method with pab and mab developed in this study could be applied to screen glyphosate-tolerant GM soybeans in soybean products.

A numerical fluid dynamic study of a high temperature operating cyclone (고온 작동 싸이클론 유체역학적 거동 전산 연구)

  • Shin, Mi-Soo;Kim, Hey-Suk;Jang, Dong-Soon
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.11
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    • pp.1033-1040
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    • 2009
  • One thing to note in cyclone operation and design is to minimize the pressure drop with the enhancement of the efficiency of dust collection. This can be facilitated by the detailed resolution of complex fluid flow occurring inside a cyclone. To this end, the main objective of this study was to obtain the detailed fluid dynamics by the development of a reliable computation method and thereby to figure out the physics of dust collection mechanism for more extreme environment caused by high temperature and pressure condition. First of all, the computer program developed was evaluated against experimental result. That is, the numerical calculation predicts well the data of experimental pressure drop as a function of flow rate for the elevated pressure and temperature condition employed in this study. The increase of pressure and temperature generally affects significantly the collection efficiency of fine particle but the effect of pressure and temperature appears contrary each other. Therefore, the decrease of collection efficiency caused by the high operating temperature mainly due to the decrease of gaseous density can be remedied by increase of operating pressure. After the evaluation of the program, a series of parametric investigations are performed in terms of major cyclone design or operating parameters such as tangential velocity and vortex finder diameter for dusts of a certain range of particle diameters, etc. As expected, tangential velocity plays the most important effect on the collection efficiency. And the efficiency was not affected significantly by the change of the length of vortex finder but the diameter of vortex finder plays an important role for the enhancement of collection efficiency.

Growth and Biomass Production of Fast Growing Tree Species Treated with Slurry Composting and Biofiltration Liquid Fertilizer (SCB액비가 속성수의 생장 및 biomass 생산에 미치는 영향)

  • Kim, Hyun-Chul;Yeo, Jin-Kie;Koo, Yeong-Bon;Shin, Han-Na;Choi, Jin-Young;Lee, Heon-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.2
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    • pp.206-214
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    • 2011
  • Fifteen clones of poplars, 2 clones of willows, and yellow poplar were used to evaluate the effects of 5 treatments such as SCBLF (slurry composting and biofiltration liquid fertilizer), general slurry liquid fertilizer, chemical fertilizer, groundwater, and control (no treatment) on vitality, growth performance, and biomass production. Five cuttings for each tree species were planted in 3 replications. After planting cuttings, a coppice was induced by cutting off stems at 10cm above the ground. Data were collected for first growing season and trees were harvested at the end of October. Maximum mortality rate i.e. 96% was recorded in the cuttings treated with groundwater and minimum 92% with control (no treatment). In all tree species, sprouting of stump was not differ significantly among the treatments. Total nitrogen concentrations of leaves and stump sprouts were higher in the treatment of SCBLF than the control, 26.6% and 22.9%, respectively. Biomass production was highest in the stumps treated with chemical fertilizer, $1.98Mg\;ha^{-1}\;year^{-1}$, and lowest in control ($1.34Mg\;ha^{-1}\;year^{-1}$).

ORF5a Protein of Porcine Reproductive and Respiratory Syndrome Virus is Indispensable for Virus Replication (PRRS 바이러스 ORF5a 단백질의기능학적역할)

  • Oh, Jongsuk;Lee, Changhee
    • Microbiology and Biotechnology Letters
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    • v.43 no.1
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    • pp.1-8
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    • 2015
  • In this study, a DNA-launched reverse genetics system was developed from a type 2 porcine reproductive and respiratory syndrome virus (PRRSV) strain, KNU-12. The complete genome of 15,412 nucleotides was assembled as a single cDNA clone and placed under the eukaryotic CMV promoter. Upon transfection of BHK-tailless pCD163 cells with a full-length cDNA clone, viable and infectious type 2 progeny PRRSV were rescued. The reconstituted virus was found to maintain growth properties similar to those of the parental virus in porcine alveolar macrophage (PAM) cells. With the availability of this type 2 PRRSV infectious clone, we first explored the biological relevance of ORF5a in the PRRSV replication cycle. Therefore, we used a PRRSV reverse genetics system to generate an ORF5a knockout mutant clone by changing the ORF5a translation start codon and introducing a stop codon at the 7th codon of ORF5a. The ORF5a knockout mutant was found to exhibit a lack of infectivity in both BHK-tailless pCD163 and PAM-pCD163 cells, suggesting that inactivation of ORF5a expression is lethal for infectious virus production. In order to restore the ORF5a gene-deleted PRRSV, complementing cell lines were established to stably express the ORF5a protein of PRRSV. ORF5a-expressing cells were capable of supporting the production of the replicationdefective virus, indicating complementation of the impaired ORF5a gene function of PRRSV in trans.

Molecular Characterization of the Bacterial Community in Activated Sludges by PCR­RFLP (PCR-RFLP 방법을 이용한 활성 슬러지의 세균군집 분석)

  • Lee Hyun-Kyung;Kim Jun-Ho;Kim Chi-Kyung;Lee Dong-Hun
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.307-312
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    • 2004
  • Diversity of the bacterial communities and the relation between community structure and components of waste­water were analyzed by 16S rRNA-based molecular techniques. Clone libraries of the 16S rDNAs from the sludges were constructed by PCR and cloning. The 1,151 clones from a sludge sample of sewage treatment plant were clustered into 699 RFLP phylotypes and the 1,228 clones from the wastewater disposal plant of chemical industry were clustered into 300 RFLP phylotypes. Shannon-Weiner diversity indices of two sampling sites were 8.7 and 6.1, indicating that the bacterial community structure of sewage treatment plant was more diverse than that of wastewater disposal plant of chemical industry. Forty clones belonging to predominant RFLP types were selected and sequenced. Seventy percent (28 clones) of the sequenced clones were related to the uncultured bacteria in public databases. The ${\beta}-Proteobacteria$ dominated in the bacterial communities of investigated two sludge samples. 16S rDNA sequences of the sewage treatment plant were similar to those of other activated sludges, while the bacterial community in wastewater disposal plant of chemical industry rep­resented the strains identified from high-temperature, anaerobic, hydrocarbon-rich, and sulfur-rich environ­ments. This result suggested that bacterial communities depended upon the components of wastewater.