Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Detection of Streptococcus mutans in Saliva using Monoclonal Antibodies

단일클론항체를 사용한 타액 내 Streptococcus mutans 검출

  • Lee, Minjeong (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Lee, Daewoo (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Miah (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Lee, Kyungyeol (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Baik, Byeongju (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Yang, Yeonmi (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Jaegon (Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
  • 이민정 (전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소) ;
  • 이대우 (전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소) ;
  • 김미아 (전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소) ;
  • 이경열 (전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소) ;
  • 백병주 (전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소) ;
  • 양연미 (전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소) ;
  • 김재곤 (전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소)
  • Received : 2014.09.02
  • Accepted : 2014.11.24
  • Published : 2015.02.28
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Abstract

The purpose of this study was to determine the usefulness of a detection method for Streptococcus mutans in saliva with monoclonal antibodies developed targeting Ag I/II and glucosyltransferases (gtf B, gtf C and gtf D) in Streptococcus mutans. In the three groups tested (adults, minors, and minors under orthodontic treatment), the results of the DMFT scores, the colony density (CFU/mL) in their saliva was measured using $Dentocult^{(R)}$-SM strip mutans, polymerase chain reaction was performed to test whether Streptococcus mutans and Streptococcus sobrinus were present, and Streptococcus mutans detecting tests performed in their saliva using four types of monoclonal antibody were collected. In conclusion, it was demonstrated that the Streptococcus mutants plays more important role in forming dental caries compared to Streptococcus sobrinus, and that the monoclonal antibodies against glucosyltransferases (gtf B, gtf C, gtf D) and Ag I/II of Streptococcus mutans are superior in detecting Streptococcus mutans to $Dentocult^{(R)}$-SM strip mutans or polymerase chain reaction.

본 연구의 목적은 Streptococcus mutans의 Ag I/II와 glucosyltransferase에 대해 개발된 단일클론항체를 사용한 타액 내 Streptococcus mutans 검출의 효용성을 평가하기 위함이다. 세 군(성인, 미성년자, 그리고 교정치료중인 미성년자)을 대상으로 DMFT, $Dentocult^{(R)}$-SM strip mutans를 이용한 타액내의 colony density (CFU/mL), polymerase chain reaction을 이용한 Sreptococcus mutans와 Sreptococcus sobrinus의 존재여부, 그리고 4가지 단일클론항체를 사용한 타액 내 Streptococcus mutans 검출 실험을 시행하였다. 그 결과 Streptococcus mutans가 Streptococcus sobrinus보다 치아우식 형성에 더 주요하게 작용한다는 것을 입증하였으며, glucosyltransferases (gtf B, gtf C, gtf D)와 Ag I/II에 대한 단일클론항체들의 Streptococcus mutans를 검출해 내는 능력이 $Dentocult^{(R)}$-SM strip mutans나 polymerase chain reaction을 사용한 검출법보다 뛰어남을 보였다.

Keywords

References

  1. Petersen PE : Challenges to improvement of oral health in the 21st century the approach of the WHO Global Oral Health Programme. Int Dent J, 54:329-343, 2004. https://doi.org/10.1111/j.1875-595X.2004.tb00009.x
  2. Korea National Oral Health Survey, 2012. Assessment service. Available from URL: http://www.google.co.kr/url?url=http://www.dibrary.net/jsp/download.jsp%3Ffile_id%3DFILE-00008155087&rct=j&frm=1&q=&esrc=s&sa=U&ei=zgZyVKOtLMSVmwXkyIG4CQ&ved=0CBMQFjAA&usg=AFQjCNHlhFCUrl38tP_gbB0uAwQvwUUNw (Accessed on November 23, 2014)
  3. Keyes PH, Jordan HV : Factors influencing the initiation transmission and inhibition of dental caries. In: Mechanisms of Hard Tissue Destruction. Academy Press, NY, 261-283, 1963.
  4. Selwitz RH, Ismail AI, Pitts NB : Dental caries. Lancet, 369:51-59, 2007. https://doi.org/10.1016/S0140-6736(07)60031-2
  5. Marsh PD : Microbiological aspects of the chemical control of plaque and gingivitis. J Dent Res, 71: 1431-1438, 1992. https://doi.org/10.1177/00220345920710071501
  6. Ajdic' D, McShan WM, McLaughlin RE, et al. : Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc Natl Acad Sci USA, 99:14434-14439, 2002. https://doi.org/10.1073/pnas.172501299
  7. Jenkinson HF, Demuth DR : Structure, function and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol, 23:183-190, 1997. https://doi.org/10.1046/j.1365-2958.1997.2021577.x
  8. Taubman MA, Nash DA : The scientific and publichealth imperative for a vaccine against dental caries. Nat Rev Immunol, 6:555-63, 2006. https://doi.org/10.1038/nri1857
  9. Rationale and Evidence for the International Caries Detection and Assessment System (ICDAS II). Assessment service. Available from URL:https://www.icdas.org/uploads/Rationale%20and%20Evidence%20ICDAS%20II%20September%2011-1.pdf (Accessed on November 23, 2014)
  10. Kohler B, Andreen I, Jonsson B : The earlier the colonization by mutans streptococci, the higher the caries prevalence at 4 years of age. Oral Microbiol Immunol, 3:14-17, 1988. https://doi.org/10.1111/j.1399-302X.1988.tb00598.x
  11. Okada M, Soda Y, Hayashi F, et al. : PCR detection of Streptococcus mutans and S. sobrinus in dental plaque samples from Japanese pre-school children. J Med Microbiol, 51:443-447, 2002. https://doi.org/10.1099/0022-1317-51-5-443
  12. Okada M, Soda Y, Hayashi F, et al. : Longitudinal studies on dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-schoolchildren. J Med Microbiol, 54:661-665, 2005. https://doi.org/10.1099/jmm.0.46069-0
  13. Jenkinson HF, Demuth DR : Structure, function and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol, 23:183-190, 1997. https://doi.org/10.1046/j.1365-2958.1997.2021577.x
  14. Nakano K, Tsuji M, Nishimura K, et al. : Contribution of cell-surface protein antigen PAc of Streptococcus mutans to bacteremia. Microbes Infect, 8:114-121, 2006. https://doi.org/10.1016/j.micinf.2005.06.005
  15. Brandtzaeg P : Overview of the mucosal immune system. Curr Top Microbiol Immunol, 146:13-25, 1989.
  16. Rozen R, Bachrach G, Bronshtetyn M, et al. : The role of fructans on dental biofilm formation by Streptococcus sobrinus, Streptococcus mutans, Streptococcus gordonii and Actinomyces viscosus. FEMS Microbial Lett, 195:205-210, 2001. https://doi.org/10.1111/j.1574-6968.2001.tb10522.x
  17. Carlsson J : Microbial aspects of frequent intake of products with high sugar concentrations. Scand J Dent Res, 97:110-114, 1989.
  18. Russell MW, Hajishengallis G, Childers NK, et al. : Secretory immunity in defense against cariogenic mutans streptococci. Caries Res, 33:4-15, 1999. https://doi.org/10.1159/000016490
  19. Taubman MA, Smith DJ : Significance of salivary antibody in dental disease. Ann N Y Acad Sci, 694:202-215, 1993. https://doi.org/10.1111/j.1749-6632.1993.tb18354.x
  20. Gregory RL, Michalek SM, Shechmeister IL, et al. : Function of anti-streptococcus mutans antibodies : anti-ribodomal antibodies inhibit acid production, growth, and glucose phosphotransferase activity. Infect Immun, 45:286-289, 1984.
  21. Childers NK, Tong G, Mitchell S, et al. : A controlled clinical study of the effect of nasal immunization with a Streptococcus mutans antigen alone or incorporated into liposomes on induction of immune responses. Infect Immun, 67:618-623, 1999.
  22. Liu C, Fan M, Bian Z, et al. : Effects of targeted fusion anti-caries DNA vaccine pGJA-P/VAX in rats with caries. Vaccine, 26:6685-6689, 2008. https://doi.org/10.1016/j.vaccine.2008.08.041
  23. Ma JKC, Hunjan M, Smith R, et al. : An investigation into the mechanism of protection by local passive immunization with monoclonal antibodies against Streptococcus mutans. Infect Immun, 58:3407-3414, 1990.
  24. Shi W, Jewett A, Hume WR : Rapid and quantitative detection of Streptococcus mutans with speciesspecific monoclonal antibodies. Hybridoma, 17:365-371, 1998. https://doi.org/10.1089/hyb.1998.17.365
  25. Wennerholm K, Emilson CG : Comparison of Saliva-Check Mutans and Saliva-Check IgA Mutans with the Cariogram for caries risk assessment. Eur J Oral Sci, 121:389-393, 2013. https://doi.org/10.1111/eos.12069
  26. Kim CS, Kim JG, Yang YM, et al. : Detection of salivary Streptococcus mutans levels using monoclonal antibodies. J Korean Acad Pediatr Dent, 37:186-192, 2010.
  27. Ikebe K, Imazato S, Izutani N, et al. : Association of salivary Streptococcus mutans levels determined by rapid detection system using monoclonal antibodies with prevalence of root surface caries. Am J Dent, 21:283-287, 2008.
  28. Cho PK, Park SS : A study on analysis of the Relationship Oral bacteria to dental caries experience in disabled student's: in Gyeong-nam. J Korean Soc Dent Hyg, 8:115-125, 2008.
  29. Chia JS, Lin SW, Yang CS, et al. : Antigenicity of a synthetic peptide from glucosyltransferases of Streptococcus mutans in humans. Infect Immun, 65:1126-1130, 1997.
  30. Nishikawara F, Katsumura S, Ando A, et al. : Correlation of cariogenic bacteria and dental caries in adults. J Oral Sci, 48:245-251, 2006. https://doi.org/10.2334/josnusd.48.245
  31. Gu F, Lux R, Anderson MH, del Aguila MA, et al. : Analyses of Streptococcus mutans in saliva with species-specific monoclonal antibodies. Hybrid Hybridomics, 21:225-232, 2002. https://doi.org/10.1089/153685902760213822

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