• Membrane Journal
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• v.34 no.2
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• pp.124-131
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• 2024

Viruses have various applications in the biopharmaceutical industry. They are used in pesticide production, production of vaccines, gene transfers, cancer therapeutics, and more. The downstream processing of viruses is an essential step for their biological and pharmaceutical applications. Among the various processes, the purification of viruses is critical. Membrane chromatography plays a vital role in this process. While ion exchange membrane chromatography is a primarily used method, it has various limitations regarding size exclusion and insufficient purification. Also, it cannot be applied to the rapidly changing strains of viruses such as influenza. This review examines various improved methods of membrane chromatography or alternatives. It focuses on purification, viral recovery rates, and scalability of the methods.

• Nuclear Engineering and Technology
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• v.28 no.1
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• pp.93-101
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• 1996

수소동위원소의 실험과 취급 및 삼중수소 제거시설의 운용이나 더 나아가서 핵융합소재의 관리기술을 위해서는 수소동위원소분만 아니라 그들의 이성질체들에 대한 정성 및 정량분석이 필수적이며 이를 위해 기체크로마토그래피 분리법이 중요한 분석수단으로 활용되어오고 있다. 본 연구에서는 고순도 $H_2및$ $D_2의$ 수소동위원소 기체를 대상으로 하고 상용의 기체크로마토그래피 분석기를 사용하여 분리컬럼 분위기를 액체질소온도로 유지하고 헬륨 케리어기체를 특별제작된 진공-시료주입장치를 통해 펄스주입하여 크로마토그래피 분리실험을 수행하였다. 10%함량의 염화망간으로 부분 비활성화 시킨 산화알루미늄을 고정상으로 선정하여 이성질체의 분리를 억제할 수 있었으며 흡착후 분리용리시간이 단축된 비교적 좋은 수소동위원소 분리조건을 실증하므로서, 저농도 수소동위원소의 정량분석과 고순도 수소동위원소의 분리 및 농축기술 개발을 위한 기초를 마련하였다.

• Journal of the Korean Chemical Society
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• v.47 no.4
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• pp.401-411
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• 2003

One of the most important characteristics in the seventh educational course is an enforcement of differentiated level curriculum. Therefore, development of various different level educational subjects is needed. A new chromatographic experimental kit which is helpful for middle school students to understand chromatographic principles is needed to correct some mistakes of chromatographic part within the 6th educational course's middle school textbook. In this research, a new thin layer chromatography(TLC) experimental kit for middle school students are developed by using acid-base indicator as samples and ethyl alcohol, ethylacetate, hexane as eluents. The kit is applied to two kinds of middle school student groups(general group & genius group) and two kinds of teacher groups(science teacher & non-science teacher) and an efficacy of the new chromatographic kits are evaluated through making up some questions.

• Analytical Science and Technology
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• v.10 no.6
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• pp.408-417
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• 1997

Normal phase or reversed phase liquid chromatographic separation of some structural isomers of functionalized heterocyclic compounds has been carried out by using several different columns and various mobile phases. The optimal experimental conditions for separation of structural isomers were found on a ternary solvent system including alcohol as a modifier. This polar modifier is preferentially adsorbed onto strong adsorption site, leaving a more uniform population of weaker site that then serve to retain the sample. This 'deactivation' of the adsorbent leads to a number of improvements in subsequent separations. The optimal mobile phase system of separation were found on normal phase on structural isomers. Retention mechanism of normal phase system was also studied depending on adsorption strength between solute and stationary phase of column. However, retention factors of reversed phase system were found on hydrophobic interaction with solvophobic effect.

• Journal of the Korean Chemical Society
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• v.55 no.2
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• pp.262-267
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• 2011

The denaturing high performance liquid chromatography(DHPLC) with fluorescence detector assay is very useful tool for detecting nucleic acids. Furthermore, loop-mediated isothermal amplification(LAMP) constitutes a potentially valuable tool for rapid diagnosis of pathogenic microorganisms. In this study, we evaluated the specificity, detection limit, and sensitivity of a LAMP method and DHPLC method for rapid detection of the hepatitis b virus(HBV). As a result, the LAMP assay reported here has the advantage of rapid detection whereas, DHPLC assay has more sensitivity than other assays. These findings suggest that LAMP and DHPLC assay may be good tool for rapid diagnosis of clinical HBV infection.

• Korean Chemical Engineering Research
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• v.60 no.4
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• pp.499-505
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• 2022

This study presents a conceptual design of vanillin production from Kraft lignin. Most of the existing Kraft lignin is used as low-quality boiler fuel or discarded as wastewater, and only 2% or less of lignin has been refined into high-quality products. We propose the process developed in this study to utilize discarded Kraft lignin. The existing vanillin production concept process consisted of alkali oxidation using NaOH, filtration, chromatography, and crystallization. Chromatography, which is difficult to commercialize, was changed to a solvent extraction process. The recovery rate of vanillin of the proposed solvent extraction process is 92.9%, and the purity is 99.5%, which is similar to the existing chromatography process. The reason why the solvent extraction process showing similar results to chromatography can replace the existing chromatography process was analyzed.

• Analytical Science and Technology
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• v.7 no.1
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• pp.11-15
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• 1994

From the aerial parts of Saururus chinensis growing in Korea, four flavonoids (hyperin, isoquercitrin, quercitrin and rutin) were isolated and identified by high performance liquid chromatography, gas chromatography and thin layer chromatography. However, avicularin, which was known as one of the main constituents of flavonoid, was not detected.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.58-58
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• 1995

식물에서부터 새로운 소염작용제를 개발하기 위하여 여러식물을 대상으로 염증반응의 초기단계에서 중요한 역할을 하는 것으로 알려진 Phospholipase $A_2$에 저해활성을 갖는 물질을 검색하였고 그 중 강한 억제활성을 보인 유근피에서 유효성분을 분리하였다. 유근피의 에칠 아세테이트 분획에 대하여 실리카겔 크로마토그래피, Sephadex LH-20 크로마토그래피, 분취 박막 크로마토그래피를 수행하여 phenol성 -OH기를 갖는 활성성분인 PSH-II-84-1를 분리하였다. $^1$H-NMR 신호의 양상과 짝지움 상수 값에서 분리된 물질은 (+)-catechin 의 당 유도체로 확인되었다. $^{13}$C-NMR 자료를 분석하여 치환된 당은 D-apiofuranose로 확인되었다. 방향족환의 $^{l3}$C-NMR 신호들은 extended Huekel theory를 응용하여 얻은 net charge 계산 값과 상관시켜 할당하였다. 이상의 구조연구 결과 이 물질은 (+)-catechin-7-0-$\beta$-D-apiofuranoside로 밝혀졌다. (+)-catechin-7-0-$\beta$-D-apiofuranoside의 효소억제활성은 Type II Phospholipase $A_2$에 대하여 $IC_{50}$/이 600$\mu$M이었다.다.

• Proceedings of the Membrane Society of Korea Conference
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• 1991.10a
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• pp.45-46
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• 1991

분리막 공정은 상변화를 일으키지 않기 때문에 장치비가 저렴하며, 열 및 화학적으로 민감한 물질, 특히 생체물질 등을 다루는데 적합하다. 또한 최종 생성물질을 정제하는 방법으로는 이온교환 크로마토그래피, 전기 영동 및 친화성 크로마토그래피(affinity chromatography)가 있다. 이중 친화성 크로마토그래피는 가장 선택적이고, 단일 장치로서 기존의 분리방법에 비해 약 1000배 가까이 정제할 수 있다. 본 실험에서는 트립신 억제제인 m-aminobenzamidine을 포함하고 있는 수용성 고분자를 제조하여 트립신을 선택적으로 분리하고자 하였으며, 본 연구 필요한 분리막은 막제조가 용이하며 비용이 저렴한 cellulose acetate막을 사용하였다. CA 막의 세공크기는 겔화매질의 에탄올 농도로 조절하였다. Casting 용액의 조성은 다음 표에 간략하게 나타내었다.

• Membrane Journal
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• v.31 no.2
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• pp.145-152
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• 2021

While there are increasing demands on biomolecules separation, resin chromatography lacks in terms of throughput and membrane chromatography is an alternative with high binding capacity and enhanced mass transfer properties. Unlike typical membrane processing, where the performance can only be empirically assessed, understanding how mechanisms work in membrane chromatography is decisive to design biospecific processing. This short review covers three separation mechanisms, including affinity interaction modes for selectively capturing bulk molecules using biospecific sites, ion exchange modes for binding biomolecules using net charges and hydrophobic interaction modes for binding targeted, hydrophobic species. The parameters in designing membrane chromatography that should be considered operation-wise or material-wise, are also further detailed in this paper.