• Korean Journal of Food Science and Technology
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• v.16 no.4
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• pp.375-382
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• 1984

The fatty acid compositions of lipids from five local varieties and three imported varieties of soybean were determined. Total and free lipids of the samples were extracted with chloroform-methanol mixture (2:1, v/v) and ethyl ether, respectively. The ether-extracted samples were extracted again with water saturated n-butanol to obtain bound lipids. The average percent contents of the lipid fractions were 21% (total), 18% (free), and 3.3% (bound). The results indicated that seven fatty acids (10:0, 12:0, 14:0, 16:0, 17:0, 20:0 and 22:0) were identified as minor fatty acids for all the varieties examined in this study. The relative contents of 18:2 were the highest (50.1-59.7%) in all the lipid fractions, and the contents in total and free lipids were in-fluenced by the local varieties. Little difference in fatty acid composition was noted between the total and free lipids of the samples. Total and free lipids contained more 18:1 (about 10%) and less 16:0 (about 40%) and 14:0 (about 34%) than did the bound lipids. The relative percent contents of 18:3 in the total, free and bound lipids were on average 8.9, 9.0, and 7.3 percents. The content in the total lipids of Gwanggyo varieties showed the highest level (11.1%), and in the bound lipids of Eundaedoo varieties the lowest (6.0%). In case of the total and free lipids, those varieties which showed higher relative contents of 18:1 generally had lower relative contents of 18:2 and 18:3.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.9
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• pp.1320-1324
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• 2005

Soybean (Glycine max L.) is an increasingly important food source and functional food. Platelet aggregation plays an important role in thrombogenesis and atherosclerosis. Here, we studied the anti-platelet aggregating effects of solvent extracts from Korean soybean varieties and isoflauone derivatives. Nine Korean soybean varieties were extracted by solvents (methanol and buthanol and their extracts was investigated for the inhibition against tile aggregation of washed rabbit platelets induced by collagen or thrombin. Maximal inhibition of buthanol extracts against platelet aggregation induced by collagen was $95\%$ in Black-kong and Jinpum - kong. The potency of their inhibition was in the following order : Black > Jinpum > Bokwang > Hwangkum > Pureun > Malli > Danbaek > Danyeob > Jangsu - kong. The Black - kong only seemed to produce the maximal inhibition against platelet aggregation induced by thrombin. Total isoflavone content measured was Jinpum-kong ($1347.8{\mu}g/g$) and Black-kong ($918.7{\mu}g/g$). Maximal inhibition of isoflavone derivatives against platelet aggregation induced by collagen was $97\%$ in genistein. The potency of their inhibition was in the following order: genistein>daidzein>genistin. The isoflavone derivatives did not affect the platelet aggregation induced by thrombin. However, Black-kong cortex seemed to Produce the optimal inhibition against platelet aggregation induced by collagen. These results suggest that Black-kong and Jinpum-kong may be a good source for antiplatelet agents, and their antiplatelet effect be related to tile content and the chemical structure with the number of -OH group and the attached glycoside in the isoflavone derivative.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.07a
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• pp.46-47
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• 1988

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.179-185
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• 1984

These studies were carried out to evaluate the feasibility of using Alkali Digestibility Value (ADV) of soybean seeds as a selection criterion for cooking quality of soybean grain with rice, and to find out an optimal testing condition, varietal differences in ADV and the effect of cultural environment on the ADV, and its mode of inheritance. The ADV test was achieved succesfully by soaking the soybean grain for 24 hours in 25 to 30% KOH solution at 25 to $30^{\circ}C$. Varietal differences in the ADV were conspicuous, but cultural conditions affected the ADV little. All collected lines for cooking with rice showed high ADV and all the small grain cultivars introduced from abroad had low ADV, while recommended cultivars showed great variation in ADV. The characteristic of low ADV in soybean grains was controlled by single dominant gene.

• Research in Plant Disease
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• v.16 no.2
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• pp.176-182
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• 2010

Occurrence of plant diseases is dependent on various factors in the agricultural system. Due to recent extensive environmental climate changes, i.e. global warming, agricultural systems such as planting dates and cultivars are being affected. Gradual transition in disease occurrence and incidence in the agricultural fields can also be affected by direct and/or indirect environmental changes. In this study, we evaluated disease occurrence and incidence in soybean plants to investigate whether it could be related with cultivars, planting dates and geographical differences in Korea in 2008. Soybean cultivars including 'Taekwang', 'Pungsan', 'Cheongja 3', 'Saeol', and 'Dawon' were planted in four different dates, May 15, June 1, June 15, and June 30, in two locations, Suwon, and Naju. Soybean diseases such as wild fire and bacterial pustule were mainly found depending on cultivars, planting dates, and areas. Wild fire occurred severely on cv. 'Taekwang' while bacterial blight did on cv. 'Dawon' among tested cultivars. Disease developments of wild fire and bacterial blight generally decreased in delayed planting regardless of cultivars.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.278-286
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• 2021

Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.140-145
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• 2007

This study was carried out to develop effective test method by soybean stink bug and to screen resistant genetic resources against soybean stink bug. The damage pod rate by stink bug showed 40% of most soybean varieties and was selected about 10% low of 10 varieties by 298 variety and degree in soybean at first year Stink bug damage rate research for 102 varieties that stink bug damage rate lowed at first year showed 10% low of 12 varieties and from 20% to 30% of the other varieties. So testing material is "Ilpumgeumjeongkong" to develop for effective test method soybean stink bug and result for stink bug damage rate research of according to growth stage showed rapidly high more full seed than full pod. Full seed stage (R6) was highest to 35.5% for stink bug damage rate. Result of resistant genetic resources selection according to stink bug damage pod rate was lowed of best to 10.3% for "Peking, Sorogkong, Hwangsaegjunjeari and Sobaeknamulkong" in the order. Also, stink bug damage seed rate was similar too. So "Peking, Sorogkong, Hwangsaegjunjeari and Sobaeknamulkong" were thought resistant variety against stink bug. Additional study carried out with "Peking and Sorogkong" so that concretely investigate about stink bug's refuse reaction. This result showed 10.0% for Peking and 14.2% for Sorogkong at R6 stage. But, damage pod rate was rapidly lowed.

• Journal of the East Asian Society of Dietary Life
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• v.12 no.2
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• pp.167-172
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• 2002

음식의 재료를 얻는 데 있어 그 편리함은 사람들의 음식습관에 영향을 주기 때문에, 본 논문에서는 먼저 대만 고추재배의 품종개량과 생산현황으로부터 전반적인 이해를 하고자 하며 나아가 신선한 생 고추 및 고추관련 가공식품, 예를 들어 고추장, 고추콩짜개된장(랄두판장. 두판: 콩짜개, 물에 불리거나 싹튼 콩), 고추기름, 말린고추 등을 전통 요리 속에서 응용하는 방법에 대해 조사하였다. 또한 식생활 속에서 고추가 끼친 영향을 연구하고 나아가 대만 음식 문화의 변화와 발전에 대해 탐구하였다. 음식문화의 발전은 분명 음식 재료의 생산과 사회.경제적 배경의 영향을 받는다.

• Journal of Life Science
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• v.22 no.4
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• pp.524-531
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• 2012

Soybean is one of the most common food materials causing food hypersensitivity reactions in Korea. In this study, we have investigated the effect of roasting and fermentation on the allergenicity of soybean. Three kinds of soybean ($Daepung$, $Daewon$, and $Taegwang$) were prepared as raw, roasted, and fermented by $Bacillus$ $subtilis$ GSK 3580, and then their proteins were extracted. The proteins were separated using SDS-PAGE, and the detection of IgE specific to soybean proteins was performed by immunoblotting using 7 sera of soybean allergy patients and non-allergic control individuals. Serum specific IgE to soybean was measured by ELISA. The SDS-PAGE of raw soybean proteins showed various-sized bands ranging from 9 to 76 kDa, which are known as major allergens. In particular, 9, 21, 34, 52, 72, and 76 kDa proteins are known as LTP, Kunits trypsin inhibitor, $Gly$ m Bd 30K, ${\beta}$-subunit, ${\alpha}$-subunit, and ${\alpha}$'-subunit of ${\beta}$-conglycinin, respectively; these are major allergens in soybean. In contrast, only peptides of less than 35 kDa were found in roasted and fermented soybeans. IgE immunoblot analysis of three roasted species of soybeans commonly detected at 38-40 kDa and 10-15 kDa. The protein bands in fermented soybean showed very weak signals or were not detected. In addition, the reactivity of most patients' sera to soybean was decreased after roasting and fermentation. With these results, it may be concluded that the allergenicity of soybeans is reduced by the roasting and fermentation processes. It is supposed that allergenic proteins in soybean were degraded by heat treatment methods and proteolytic enzymes were secreted from fermenting microorganisms.

• Journal of Life Science
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• v.16 no.2 s.75
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• pp.289-296
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• 2006

In order to establish highly efficient gene transfer condition at early stage of soybean transformation, various experiments were performed and compared their efficiencies by transient GUS analysis; those conditions are genotype determination of Korean soybean cultivars for amenability to Agro-infection, appropriate agar and selective agent concentration, orientation of explant placement, hormone pre-culture, and liquid selection condition. In the genotype screen of Korean soybean varieties, 14 amenable genotypes were selected. For efficient Agrobacterium washing, cefotaxime was chosen and hygromycin at the concentration of 10 and 15 ppm was used as selection agent in the media. Agar concentration was slightly better in 0.6% and 0.8% for both shoot and callus formation, and explant placement with adaxial side down showed high frequency of GUS expression. For wounding treatment, oriental needle was efficient than scalpel for shoot formation and gene transfer. To increase the frequency of gene transfer, hormone pre-treatment was applied. BA at the concentration of 5 and 10 ppm resulted in better survival at the late stage of selection in shoot elongation media. Selection in liquid media after hormone pre-treatment seemed to be effective to remove the escaped non-transformants at early stage of procedure. Considering the results obtained, Eunhakong could be the first choice as a material for soybean transformation among Korean soybean genotypes.