• Title/Summary/Keyword: 콜레라균

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해양미생물의 세계 - '바다 + 어패류'와 콜레라

  • Choe, Dong-Han
    • The Science & Technology
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    • v.35 no.8 s.399
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    • pp.85-85
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    • 2002
  • 콜레라균은 0.3~3%의 염분에서 살 수 있으며 2% 정도의 염분에서 최적성장한다. 콜레라의 감염경로는 굴, 게, 및 생선 등 어패류를 날 것으로 섭취할 때 발생된다.

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병원성 비브리오균의 용혈독소와 단백분해효소에 관한 연구

  • 박미연;김현진;장동석
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2002.10a
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    • pp.177-178
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    • 2002
  • 병윈성 비브리오균의 병원인자에는 hemolysin, protease, phospholipase A2, siderophore 외에도 콜레라균 만이 생산하는 cholera toxin 등이 있다. 이 중에서도 대부분의 병원성 비브리오균에서 생성되는 대표적인 인자는 hemolysin과 protease로 알려져 있다. Hemolysin은 혈액을 분해하는 독소로서 병원성 비브리오균의 분리ㆍ동정에 널리 이용 되고 있다. Hemolysin은 균의 배양초기에서 부터 서서히 생성되기 시작하여 대수증식기 말에 최대의 활성을 나타내며 안정기에 접어들면서 활성이 급격히 감소되는 것으로 보고되고 있다 (Kim et al., 1997). (중략)

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A Study on the Validation system of Detection for Biological Agents Using Real-Time PCR (실시간 중합효소 연쇄반응을 활용한 생물작용제 검증시스템 연구)

  • Cha, Younggil;Koo, Bonwoo;Kim, Seongjoo;Kim, Namil;Park, Hanoh
    • Journal of the Korea Institute of Military Science and Technology
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    • v.20 no.5
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    • pp.726-732
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    • 2017
  • Bacillus anthracis, Vibrio cholerae, Variola virus and Shigella dysenteriae are classified as category A and B biological weapons. In this study suggest that 4 genes of Bacillus anthracis, 2 genes of Vibrio cholerae, 1 gene of Variola virus and 1 gene of Shigella dysenteriae were detective 50~500 fg of target DNA per reaction using real-time PCR based assay. Also analytical specificity did not show any cross-reactivity with other related bacteria. Reliable and one reaction could be effective early diagnostic and treatment for detection of unknown samples.

해수에서 분리된 V. cholerae non-O1 FM-7의 용혈독소에 관한 연구

  • 배기정;박미연;김기현;장동석
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.217-219
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    • 2001
  • 비브리오균은 현재까지 약 30여종이 알려져 있는 그람음성의 종속영양세균으로, 이 중 12종이 사람에게 질병을 일으킨다고 보고되고 있으며, 우리 나라에서 문제가 종종 발생하는 비브리오균은 패혈증 비브리오, 장염 비브리오 그리고 V.cholerae non-O1을 들 수가 있다. V. cholerae O1과 형태학적, 생화학적, 생물학적 성상은 일치하지만, 콜레라균의 항혈청에는 응집하지 않는 일군의 세균을 V. cholerae non-O1이라고 부른다. (중략)

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Molecular Biological Characteristics of Vibrio cholerae O1 Isolated from Diarrheal patients in the Gyeongbuk province. (최근 경북지역 설사환자 검체에서 분리된 Vibrio cholerae O1의 분자생물학적 특성)

  • 이상조;이복권;이건주;이희무
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.334-341
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    • 2003
  • This study was carried out to investigate the cause of cholera outbreak in Gyeongbuk province in 2001.90 strains of Vibrio cholerae O1 El Tor serotype Inaba were isolated from diarrheal patients. By multiplex-PCR, all of the isolated strains revealed positive for detection ctxA, hlyA and tcpA genes. There were DNA sequence difference of the cholera-toxin subunit A gene and subunit B gene between isolated V. cholerae O1 and the strain of GenBank. In analysis of PFGE patterns, all of the isolated strains were showed the same DNA fragments. We also collected plankton samples in the east coast of Gyeongbuk to isolate V. cholerae O1 and V. cholerae O139 from August to October 2002. The samples were examined to detect the rfb gene and cholera-toxin gene by multiplex-PCR. The cholera-toxin gene was detected and then we tried to isolate V. cholerae O1 and V. cholerae O139, but they were not isolated.

Relationship between Pathogenic Vibrios and Zooplankton Biomass in Coastal Area, Korea (병원성 비브리오균과 동물성 플랑크톤과의 관계에 관한 연구)

  • CHANG Dong-Suck;KIM Chang-Hoon;YU Hong-Sik;KIM Shin-Hee;JEONG Eun-Tak;SHIN Il-Shik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.5
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    • pp.557-566
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    • 1996
  • An ecological study on pathogenic vibrios was done in the aquatic environments of southern coast of Korea during summer in 1995, to investigate the distribution and relationship between pathogenic vibrio and zooplankton. Furthermore, special emphasis was given to study on the efforts of zooplankton existence on the wintering of Vibrio cholerae in the aquatic region in Korea. During the study period, pathogenic vibrios were isolated from the samples such as V. parahaemolyticus, V. vulnificus, V. mimicus, and V. cholerae non O1, but V. cholerae O1 was not detected in any sample submitted in this study. Adsorption ratio of V. parahaemolyticus onto zooplankton was higher than that of E. coli. The efficiency of adsorption was found to be on the concentration of NaCl and other ions found in sea water. For example, adsorption ratio of V. parahaemolyticus were $75\%\;at\;5\%_{\circ}$ of NaCl solution and $55\%$ at same salinity of diluted sea water, but those were decreased as $20\%\;and\;7\%\;at\;15\%_{\circ}$ salinity of NaCl solution and diluted sea water, respectively. In addition, survival period of pathogenic vibrio was extended in the presence of live copepods at $25^{\circ}C$, but zooplankton existence has no significant effect on the survival rate at $5^{\circ}C$ in closed microcosm and also microalgae and dead copepods do not affect on the survival of V. parahaemolyticus. According to these experimental results, zooplankton has positive effects on the growth and survival rate of pathogenic vibrios in sea water during the summer season, but copepods have no significant effects on the growth and survival rate of them in winter season in Korea. Finally, authors suggest that V. cholerae is not able to over winter with zooplankton in adjacent sea water in Korea.

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A Bacteriological Study on the Sea Waters and Oyster in Puk Ma , Korea (북만의 해수 및 굴의 세균학적 특성)

  • Park, Jong-Duck;Jeong, Woo-Geon
    • The Korean Journal of Malacology
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    • v.14 no.1
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    • pp.19-26
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    • 1998
  • 북만 해수의 물리 화학적 및 미생물학적 특성과 북만에서 양식되고 있는 굴에 대한 세균학적 수질을 조사하여 수출용 패류생산지정 해역수질에 합당한가를 파악함과 동시에 지표세균의 조성, 병원성 세균 등을 조사한 결과를 요약하면 다음과 같다.사 기간중 불반 해수의 수온은 6.8 -23.$0^{\circ}C$, 투명도는 2.7-5.9m, COD 1.55-2.12 mg/1, DO 6.3-10.1 mg/1, 용존질소 1.63-20.87 $\mu\textrm{g}$-at/1, 인산염 0.16-1.66 $\mu\textrm{g}$-at/1, Chlorophyll. a는 1.04-6.78 mg/ ㎥범위였으며 염분농도는 31.57-34.47$\textperthousand$였다. 북만 해수의 세균학적 수질은 통영항, 인평포, 법송포 및 북신만 안쪽의 일부 해역을 제외하고는 수출용 패류의 생산해역의 수질기준에 합당하였다. 대장균군의 최확수는 양식을 하지 않는 조사지점 1과 2를 제외하고 해수 100ml 당 < 3.0-4,600의 범위였으며 230을 초과하는 시료의 비율은 10.7%였고, 분변계 대장균의 최확수는 <3.0-1,100의 범위였으며 43을 초과하는 시료의 비율은 9.5%로 한계치 10%를 약간 상회하였다. 대장균군의 분류겨로가 Escherichia coli가 약 52%나 되어 오염원의 주류가 분변오염임을 알 수 있었다. 살모넬라, 시겔라, 콜레라균 등 수인성 병원세균은 검출되지 않았다. 병원성 비브리오균은 여름철인 6-8월 사이에는 시료의 9-22%에서 양성으로 나타났다. 굴 내에 생균수는 2.0 x $10^{2}$-1.6 x $10^{3}$범위였고 대장균군의 최확수는 <18 - 16,000, 중앙치는 176, 분변계 대장균은 <18-2,400, 중앙치는 었다.

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Distribution of Virulence Factors of Vibrio cholerae non-O1 and non-O139 Isolated from Korea (한국에서 분리된 Vibrio cholerae serovar non-O1 및 non-O139 병독 인자의 분포)

  • 성희경
    • Korean Journal of Microbiology
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    • v.35 no.3
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    • pp.248-252
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    • 1999
  • The PI-oduction of virulence factors such as cholera toxin, heinolysin and hemagglutinin in V cliolerae non-01 and non-0139 were examined. Among 65 strains isolated from environmental and clinical blood sources, 29 (14.6%) strains produced hemolysin only, 35(53.9%) sh.ains produced both hemolysin and hemagglutinin. From one 037 slrain isolated from environmenl, cholera toxin, ctx gene, hemolysin, and hemagglutinin were detected. All of the strains isolated from clinical and environmental sources showed hemolytic activity against human 0 group e~ythrocytes. In inhibition patterns of heinagglotination, 5 of 18 clinical strains (27.8%) were inhibited by less than 1% mannose and galactose, while, among the 47 environmental isolates. hose paltems by less than 1% mannose and galactose 55.4% wel-e inhibited. Thel-ehre, exohamagglutinin positive rate was high in clinical blood isolates but in environnlental sources, the rate was almost similar lo ihe rate or endohemagglutinin positive. These results indicaled that V cholerae non-01 and non-0139 produced various virnlence factors such as cholera toxin, hemolysin, and hemagglutinin but not a single factor. Further studies are need for epidemiological or bacteriological shtdies of V cholerae 037 isolated from environment.

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동물약사

  • 한국동물약품협회
    • 동물약계
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    • no.89
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    • pp.2-4
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    • 2003
  • [ $\cdot$ ]동물용의약품등 수입관리자 신규 승인 $\cdot$동물용의약품 제조$\cdot$수입업 폐업 신고 $\cdot$동물용의약품 제조$\cdot$수입업 휴업 신고 $\cdot$동물용의약품등 제조업 허가사항 변경 $\cdot$동물용의약품등 수입자 확인사항 변경 $\cdot$동물용의약품 품질관리우수업체 신규 지정 $\cdot$동물용의약품 품질관리우수업체 변경 지정 $\cdot$가축전염병예방법시행규칙 개정(안) 입안예고 $\cdot$식육 중 잔류물질검사요령 개정(안) 입안예고 $\cdot$동물의균독주관리요령 개정(안) 입안예고 $\cdot$동물용의약품안전사용기준 개정(안) 입안예고 $\cdot$돼지콜레라 예방약 생산$\cdot$유통 허용

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