• Korean Journal of Organic Agriculture
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• v.28 no.4
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• pp.591-604
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• 2020

The objectives of this study were to find out the best condition of extracting methods of limonene from citron and to determine effects of limonene on immune modulation activity by measuring cytokine secretion using RAW 264.7 mouse macrophage cells. When distilled water was used as a solvent instead of organic solvents to extract limonene from citron, addition of refluxing process to simultaneous steam distillation extraction method was found to be much effective in extracting limonene. However, it required longer extraction time than using other organic solvents. Limonene extracts showed increased IL-β and IL-6 but decreased the TNF-α gene expression in limonene concentration dependant manner. However oral administration of limonene extracts to mice did not influence significantly compared to control in in vivo experiment. It might be due to that the mice were kept in well controlled and complete environment. Limonene, a natural material from citron has been approved to have a immune-modulation activity in the present study and have a potential as a feed additive that is environmentally friendly and no harmful. Further study with protected limonene, for example, for the protection of limonene from oxidation or bypass the ruminal degradation in order consequently to increase immune-modulation activity might be useful as a further research.

• Korean Journal of Organic Agriculture
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• v.15 no.2
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• pp.207-218
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• 2007

Coming with the well-being era, consumer's demand for safe agricultural products is increasing. So, it is urgent to develop an environment-friendly rice production system. Accordingly, this study was conducted to develop an environment-friendly rice production system by using Amo known as EM(effective microorganisms) and also known as being effective in environment-friendly rice production with several other environment-friendly agricultural materials. The highest number of tillers per hill was obtained from level 2 of EM treatment (48.8 tillers per hill), while the lowest was obtained from the control plot (41.0 tillers per hill). Leaf area per hill at heading stage was the highest in level 3 of EM treatment $(3228.5cm^2)$, while control was the lowest leaf area $(2264.7cm^2)$, which is 70.2% compared to the level 3 of EM treatment. The highest effective tillers was obtained from the control (63.7%), while the lowest effective tillers was obtained from the level 3 of EM treatment (55.4%), which were treated with higher amounts of environment-friendly agricultural materials. Level 3 of EM treatment showed the highest number of panicles per hill (20.9), while the control showed the lowest number of panicles per hill (19.3). In the spikelets per panicle, level 2 of EM treatment showed the highest number of spikelets (85.2), while the control showed the lowest number of spikelets (81.9) and there was a statistically significant difference among the three levels and control. The highest grain filling ratio was obtained from the control (85.0%), while level 3 of EM treatment was the lowest grain filling ratio and there were no great difference between treatment levels. Regarding the 1000 grain weight, the control showed the highest 1000 grain weight (21.7g), which is heavier by about 1g compared to treatment levels. Level 2 of EM treatment showed the highest rough rice yield per 10a, while level 3 of EM treatment was the lowest and they also showed a statistically significant difference among treatment levels.

• Applied Chemistry for Engineering
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• v.33 no.4
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• pp.335-342
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• 2022

In the agricultural sector where the fossil fuels are primary energy resources, the current global energy crisis together with the dissemination of smart farming has led to the new phase of energy pattern in which the electricity demand is growing faster particularly. Therefore, the fuel cell combined heat and power system, coupling the environmentally friendly fuel cell to biomass treatment and feeding, can be regarded as the most effective energy system in agriculture. In this mini-review, we discuss the R&D trend of the fuel cell combined heat and power system aimed at utilizing agricultural by-products as fuels and highlight the issues in terms of the process configuration and interconnection of individual processes.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.193-205
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• 2023

Botanical insecticides derived from plant extracts exhibit repellent, antifeedant and enzyme-inhibiting activities against insect pests. Among such pests, phytophagous mites are major threats to horticultural crops. Botanical extracts derived from neem, chrysanthemum, and shrubby sophora are employed as field acaricides. These botanical extracts have low toxicities against natural enemies of the insect pests and, thus, are valuable in pest management. This review focuses on the potential for botanical extracts in the controls of mites, with comparisons of the spectrum of activity, the lethal dose and times and their mode of action. This information will enable better formulation of botanical extracts in integrated mite control.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.1
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• pp.27-40
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• 2022

Building self-sustainable rural infrastructure and environment through smart digital agriculture technology innovation is one of the major goals of the Korean agricultural administration as a part of the nation's 4^th industry revolution. To identify areas for improving and effectively investing in the acceleration of rural development, 207 experts in the areas of crop science and smart digital agriculture technology were interviewed for their opinions and suggestions on 22 questions designed to recognize fundamental agricultural issues to be addressed and solutions to advance technology innovation and rural development. Majority of the participants expected smart digital agriculture technologies to resolve major agricultural issues and help build a better rural environment. To overcome technology gaps and resolve issues more effectively, further investment in training new technology experts and building stronger agricultural technology infrastructure is urgent, and persistent and systematic support from agricultural administration appears to be the key for accelerating the process. While the leading global groups of both public and private sectors have advanced their technologies beyond the field application stage, most of the Korean technologies remain at the early pilot stage. Aging population and lack of labor in rural areas, unknown future climate change, and challenges in sustainable rural development are expected to be resolved by smart digital agriculture technologies. Technological innovations by research institutes should be promptly deployed in the crop production field, and farm training systemically organized by local technology centers can accelerate farming revolution. Standardization of equipment and data systems is another key to the success of digitalization of food crop production and food supply chains nationwide.

• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.3
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• pp.23-29
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• 2015

The environmental friendly well being food is currently more concerning with being important for living together between human and nature worldwide by inducing the abnormal weather and global warming effect. Organic farming in Korea is slightly developed for past 100 years compared with the advanced country. However, world market of organic farming will be expanded in the future. According to holding 2015 Goesan World Organic Agriculture Industry Expo (WOAIE) from Sept. 18 to Oct. 11 organic farming in Korea will be developed. For being the safe and successful Expo, indices for 5 sectors are developed as SSaM-GG(Shared, Smart, and Mutual-Green Growth) with emphasis on reduction of accidents.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.101-108
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• 2022

Because of their specificity to target insects and relatively low toxicity to non-target organisms, insect growth regulators (IGRs) have been regarded as attractive alternatives to chemical insecticides. Commercially available IGRs are classified into juvenile hormone agonists (JHAs), ecdysone agonists (EAs), and chitin synthesis inhibitors (CSIs) according to their mode of action. Recently, JH-mediated interaction of methoprene-tolerant (Met), which is JH receptor, and its binding partners have been replicated in vitro using yeast cells transformed with the Met and FISC/CYC genes of A. aegypti. Using this in vitro yeast two-hybrid β-galactosidase assay, juvenile hormone antagonists (JHANs) have been identified from various sources including chemical libraries, plants, and microorganisms. As juvenile hormone (JH) is an insect specific hormone and regulates development, reproduction, diapause and other physiological processes, JHANs fatally disrupt the endocrine signals, which result in abnormal development and larval death. These results suggested that JHANs could be efficiently applied as IGR insecticides with a broad insecticidal spectrum. This review discuses JH signaling pathway mediated by Met and future prospects of JHANs as environmentally benign IGR insecticides.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.3
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• pp.137-146
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• 2022

Seed disinfection is a necessary process to decrease Bakanae disease in rice and is carried out at 30℃ to increase disinfection efficiency. However, the length of sprout after seed disinfection is not suitable for sowing, so additional processes such as seed soaking is needed. For this reason, this study was conducted to develop the efficient and effective seed disinfection method. When the seed disinfection finished after soaking seeds in cold water for 2 days, length of sprout was about 1.0 mm and sowing can be done without additional processes. In addition, the incidence of Bakanae disease was greatly reduced by 64% compared to conventional method. In order to find out why the newly developed method (disinfection after soaking) is more efficient than conventional method, soaked and not soaked seeds were stained using methylene blue staining. It was found that soaked seeds were more easily stained with about 50% of internal and external endosperm stained. Therefore, pesticide can be absorbed easily into internal endosperm of soaked seeds and efficiently destroy the spore of pathogen. As a results of this study, newly developed method will be useful as it can improve efficiency of agricultural work and also effectively decrease Bakanae disease compared to conventional method.

• Journal of Applied Biological Chemistry
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• v.64 no.4
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• pp.433-438
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• 2021

Polyethylene is widely used as an agricultural film, but eco-friendly technology is lacking for its decomposition. Thus, recently, much attention has been paid to develop a technology for biological polyethylene decomposition. It has been expected that several oxidation steps will be required in the biological degradation of polyethylene. First, secondary alcohol is formed on the polyethylene chain, and then the alcohol is oxidized to a carbonyl group. In the subsequent process, the carbonyl group is converted to an ester by Baeyer-Villiger monooxygenase (BVMO), and this ester bond is expected to be cleaved by lipase and esterase in the final step. In this work, we reviewed BVMO as one of the promising enzymes for polyethylene decomposition, in terms of its reaction mechanism, classification, and engineering. In addition, we also give a brief perspective on polyethylene decomposition using BVMO.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.3
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• pp.307-317
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• 2019

To develop sustainable new natural anti-aging ingredients from Korean native plants, we investigated the cultivation potential of Nymphoides indica using the eco-friendly aquaponics system, and tested the anti-aging effects from N. indica extracts. N. indica could be grown in aquaponics system using floating leaved deep water culture method, and propagated through rhizome propagation. It was confirmed that the nitrate ($80{\mu}g/mL$), potassium ($63.5{\mu}g/mL$) and water temperature ($25^{\circ}C$) greatly affected the cultivation of the N. indica. In addition, synergistic effects were found when two major components (3,7-di-O-methylquercetin-4'-O-${\beta}$-glucoside & sweroside) were present at more than about $5{\mu}g/mL$. The extract had a significant effect on the recovery of skin cells damaged by environmental pollutant such as $benzo[{\alpha}]pyrene$, ammonium nitrate, formaldehyde. It also suppressed $PGE_2$, $TNF-{\alpha}$ and COX-2, and inhibited the production of MMP-1. Taken together, the results suggested that the standardized extracts of N. indica cultivated in the aquaponics has considerable potential as a new cosmetics ingredient with an anti-aging effect.