• Title/Summary/Keyword: 치환비율

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The Rates of Synonymous and Nonsynonymous Substitutions in Sorbus aucuparia Using Nuclear and Chloroplast Genes (핵 및 엽록체 유전자를 이용한 유럽마가목에서 동의 및 비동의치환율)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.20 no.4
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    • pp.481-486
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    • 2010
  • The rates of synonymous and nonsynonymous nucleotide substitutions were studied for sequences of nuclear and chloroplast genes in Sorbus aucuparia. Results suggested that DNA evolution in this species had taken place, on average, at a slower rate in the chloroplast genes than in the nuclear genes: a rate variation pattern similar to those observed in eudicot plants. Within the nucleus, the synonymous substitution rates (Ks) (2.45-2.60) were two-fold higher than nonsynonymous substitution rates (Ka) (1.15-1.30). More notably, the values of Ks (1.20-1.26) were about six-fold higher than those of Ka (0.26-0.42) within the chloroplast genome. Ka/Ks ratios for nuclear and chloroplast genes of S. aucuparia had mean values of 0.178 and 0.056, respectively. A Ka/Ks ratio < 1 indicated negative (purifying) selection. The chloroplast genes had a lower effective number of codons (ENC) values (22.4-32.2) than those of nuclear genes (35.8-38.7). The analysis of the G+C content indicated that the chloroplast genes in this investigation had a higher preference for synonymous codons ending with A and T (G+C content range, 28.4-29.1%) where there was a slight bias toward codons ending with G+C (63.2-64.2%) in the nuclear genome.

Physical and Chemical Quality of Organic by Product Fertilizers by Composting of Livestock Manure in Korea (가축분뇨를 원료로 하는 부산물 비료의 부숙화에 따른 물리화학적 특성변화)

  • Lee, Chang-Ho;Ok, Yong-Sik;Yoon, Young-Man;Kim, Dae-Yeon;Lim, Soo-Kil;Eom, Ki-Chul;Kim, Jeong-Gyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.224-229
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    • 2006
  • Utilization of organic by-product fertilizers has many beneficial effects on agricultural activities and in aspects of the disposal of enormous amounts of livestock manure. Most of these beneficial effects are related to the improvement of soil condition, such as fertility status and physicochemical quality of soil. But, appropriate indexes are needed to effectively manage the quality of organic by-product fertilizers amended on soil. To find chemical and physical standard to control the compost quality, the changes in chemical and physical characteristics of organic by-product fertilizers during composting were investigated, and also an appropriate physical method for this end. The results showed chemical properties, such as humic acid content, OM/N ratio, cation exchange capacity and salt content, had significant relationships during the composting. The water content, particle and bulk densities, particle size and color indices, as physical properties, were also applicable factors for the quality control of compost.

Revised Rates of NPK Fertilizers Based on Soil Testing for Vegetable Crops (토양검정(土壤檢定)에 의한 채소류(菜蔬類)의 삼요소(三要素) 시비량(施肥量) 조정(調整))

  • Lee, Choon-Soo;Huh, Beom-Lyang;Song, Yo-Sung;Kwak, Han-Kang
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.2
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    • pp.85-91
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    • 1994
  • An attempt to adjust the standard levels of NPK fertilizers application was made with the analytical data of soil and the results of field experiments on 18 vegetable crops 1. There was a remarkable accumulation of available $P_2O_5$ and exchangeable K of soil in the periods of 1980~1990 comparing with 1960~1970. 2. New fertilizer recommendations based on the accumulation of soil components enabled to decrease fertilizer requirements of major vegetable crops. It was founded that if such new recommendations were adopted by the farmer, 0~6.0kg N/10a, 0.7~12.3kg $P_2O_5/10a$ and 2.0~9.0kg $K_2O/10a$ could be saved without affecting the yield of crops 3. The formulas of fertilizer application for crops were corrected in order to determine optimal rate of each farmer field according to chemical properties of soil.

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The Agricultural Utilization of Daecheong Lake Sediments and Algae (대청호(大淸湖) 저니(底泥) 및 조류(藻類)의 농업적(農業的) 활용(活用) 연구(硏究))

  • Chang, Ki-Woon;Lim, Jae-Shin;Lee, In-Bog
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.2
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    • pp.111-116
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    • 1994
  • These studies were carried out to investigate the possibility on the agricultural utilization of lake sediment collected in the Daecheong Lake. Several parameters to estimate the degree of maturity of compost by windrow system were tested during a 50 day periods, and the results were as follows : During the composting of Daecheong Lake sediment, C/N ratio decreased gradually from 18.9 at the beginning to 13.1 at the final, while CEC increased from 33.5me/100g to 62.5me/100g. The temperature of lake sediment pile was the highest as $52^{\circ}C$ at 15th day and then decreased slowly until $30^{\circ}C{\sim}40^{\circ}C$ after 50 days. The results may be suggested that Daecheong Lake sediment compost reaches an acceptable level of stability after about 50 days. In order to know the effect of the Lake sediment and its compost on the growth of tomato(Lycopersicum esculentum M.), pot experiments were performed. The appiication of lake sediment(AS) itself, sediment organic fertilizer(SOF), and NPK mixed with sediment organic fertilizer(NSF) was more effective for tomato plant height, fresh and dry weight, and chlorphyll content than those of control treatment. Also, the lake sediment compost improved the physico-chemical properties of soil such as pH, total carbon, total nitrogen, available phosphorous and CEC.

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Studies on the Phosphatic Fertilizer Application in Grassland II. Effects of phosphatic fertilizer application at the establishment and management of grassland on the botanical composition and mineral content of grasses (초지에 대한 인산질비료의 시용에 관한 연구 II. 초지조성 및 관리시에 인산질비료의 시용이 초지식생 및 목초의 무기물 함량에 미치는 영향)

  • Park, Geun Je;Lee, Pil Sang;Choe, Gi Jun;Hwang, Seok Jung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.13 no.4
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    • pp.268-273
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    • 1993
  • With a purpose of finding out the effects of phosphatic fertilizer application at the grassland establishment and management on the botanical composition, mineral content of grasses and the chemical properties of soil. a field experiment was arranged with four treatments as a randomized complete block design and lasted from September, 1989 to October, 1991 in hilly area near Suweon. The results obtained are summarized as follows; 1. The botanical composition of grassland that phosphatic fertilizer was not applied was poor and the rate of legumes was increased with phosphatic fertilizer application and the effect of phosphatic fertilizer applications was obvious at the grassland management. 2. The mineral contents, N. P, Ca, Mg of grasses were increased with phosphatic fertilizer application but the K/Ca+Mg equivalent ratio of grasses was lowered and K content and Ca/P ratio tended not to be regular. 3. The available phosphorus contents and exchangable cation, Ca, Mg, contents of grassland soil were increased with phosphatic fertilizer applications but potasium contents tended not to be regular. 4. It was necessary to apply phosphatic fertilizer for the establishment and management of grassland. The botanical composition of grassland that phosphatic fertilizer was not applied at the establishment could be corrected into good stand with continuous phosphatic fertilizer applications at the management.

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Effect of Banded Subsoil Fertilization on the Yields and N Utilization of Radish (Raphanus sativus L.) in Plastic Film Mulching Cultivation (무 재배시 질소이용률 및 수량에 미치는 토중시비 효과)

  • Yang, Chang-Hyu;Yoo, Chul-Hyun;Shin, Bok-Woo;Kang, Seung-Won
    • Korean Journal of Environmental Agriculture
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    • v.24 no.4
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    • pp.398-403
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    • 2005
  • To establish law-put fertilization technique and increase of fertilization efficiency during cultivation of plastic film mulching for plastic the improvement of soil properties, nutrition efficiency and yield by banded subsoil fertilization (BSF) using band spoty applicator was conducted at radish (Raphanus sativus L.) field in Honan Agricultural Research Institute from 1997 to 1998 for 2 years. These results were as follows. T-N, available $P_2O_5$ exchangeable Ca and K contents of soil were increased those of before experiment especially in BSF treatment. Also, the content of soil $NO_3-N$ was higher in BSF as fertilization amount is increasing than in CF (conventional fertilization). Uptake amounts of nitrogen fertilized were high in BSF during overall the growth period of plant and N utilization was high by $21.9{\sim}30.4%$ in BSF compared to in CF. The rate of fertilizer loss by rainfall was largely reduced, because all fertilizer applied was putted around the root zone. The total yields of fresh radish BSF treatments were more increased $13{\sim}37%$ than that of CF treatment.

Study on Establishment of Organic Pasture by Using Korean Black Goat and Improvement of Botanical Composition (흑염소를 이용한 유기초지조성과 식생 개선 효과)

  • Yoon, Sei-Hyung;Lim, Young-Cheol;Kim, Jong-Geun;Lee, Joung-Kyong;Choi, Sun-Ho;Cho, Nam-Chul;Choi, Ki-Choon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.3
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    • pp.227-234
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    • 2009
  • This study was carried out to determine the effect of establishment of organic pasture by hoof cultivation with Korean black goat on productivity of grassland and properties of soil. This study was conducted from August, 2005 to September, 2008 at Cheonan, Korea. Experiment was designed composing with 4 treatment : Standard (Control, T1), Grazing after application of cattle manure (T2), Application of cattle manure after grazing (T3), Application of cattle manure at half of grazing (T4). The establishment ratio of pasture in control treatment increased significantly as compared with that of organic treatment(p<0.05). The yield of dry matter (DM) of pasture in control treatment increased significantly as compared with that of organic treatment. The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF) and dry matter digestibility (DMD) of pasture were not influenced by cattle manure and grazing method. Grasses coverage ratio in grassland in second year after grassland development were higher than that of first year. The contents of organic matter (OM) and $P_2O_5$ in soil samples collected in organic treatment at the end of the experiment were higher than those of control. However, The pH, and the concentrations of CEC (Ca, Na and Mg) in soil samples collected in organic treatment were hardly influenced, as compared with those at the beginning of the experiment. This study suggests that the grasses and legumes coverage ratio in grassland can be improved by suitable pasture management.

Salts Reduction Effect of Natural Zeolite in Plastic Film House Soil (천연 Zeolite를 이용한 시설재배지 토양의 염류제거 효과)

  • Wee, Chi-Do;Li, Jun-Xi;Kim, Hong-Lim;Sohn, Bo-Kyoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.552-557
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    • 2010
  • This study was performed to investigate the effect of zeolite on the reduction of soil EC level in the plastic film house. The EC level of experimental soil was 5.0 dS $m^{-1}$ and the zeolite was applied to the soil at seven levels (0.5, 1, 2, 5, 10, 15, 20%) with three replications. The reduction degree of soil EC level showed positive tendency to the mixing ratio of zeolite. Especially, the EC level reduced rapidly from 5.01 to 2.8 dS $m^{-1}$ in the plot where zeolite was mixed by 20% 10 days after treatment. The pH level of soil was in positive relation to the mixing ratio of zeolite, contrary to the negative relation to the concentration level of water soluble Ca, Mg and phosphorus (P). The water contents of soil mixed with 15% and 20% zeolite were 14% and 17.3% respectively but it was 12.7% for control soil. Therefore, we expect natural zeolite to salts reduction agent for exchangeable cation and phosphate which is difficult to reduce by watering and other methods.

Preparation of Liquefied Cellulose and Analysis of Its Components by GC-MS Spectrometry (액화셀룰로오스의 제조 및 GC-MS에 의한 그 성분 분석)

  • 조국란;황병호;공영토;도금현
    • Journal of Korea Foresty Energy
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    • v.19 no.2
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    • pp.86-92
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    • 2000
  • The liquefactions of $\alpha$-cellulose(Sigma Chemical, C-8002, 47H0383) was prepared in the presence of phenol using sulfuric acid as a catalyst under $N_2$ gas protection at $180^{\circ}C$ for 60minutes to examine its components. The ratio of $\alpha$-cellulose to phenol was 1: 6.2(w/w), and that to sulfuric acid was 1: 0.05(g/$m\ell$). The yields of liquefaction were calculated after the liquefied mixtures were passed through 1G4 glass filter. The luquefied product of $\alpha$-cellulose was analyzed using GC-MS Spectormeter. The 12 compounds identified by GC-MS Spectrometer, of which peak area covers 54% as 2,4-dimethyl phenol, p-isopropyl phenol, 1-ethyl-3,5-dimethyl benzene, o-isopropyl phenol, (E)-2,4\` dihydroxy-stilbene, 2,2\`-methylene-bisphenol, 4,4\`-methylenebisphenol, 3-methyl-2-hydroxyphenyl-(E)-2-hydroxyl-4\`-methoxy-stilbene, 1-phyenyl-1-(4\`hydroxyphenyl)methanol phenol derivatives. From this results, the reaction pathways of the liquefaction of cellulose were proposed through electrophilic substitution reaction. Phenol as a solvent might react with the reaction intermediates as well in the cellulose liquefaction.

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Quality Changes of Retorted Samgyetang During Storage Depending on the Degassing Methods (탈기 포장 방법에 따른 레토르트 삼계탕의 저장 중 품질 변화)

  • Jang, Min Jun;Lee, Jin Ho;Lee, Keun Taik
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.19 no.2
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    • pp.67-73
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    • 2013
  • This study investigated the quality changes of Samgyetang during storage, resulting from various degassing methods used at the packaging stage. Three different samples were prepared, one without any treatment (CON), a second with reduced head space volume by squeezing the pouch (RHS), and a third by flushing with nitrogen gas (NGF). These were retorted at $120^{\circ}C$ for 65 min under the F-value of approx. 8.0, and stored at $25^{\circ}C$ for 9 months. The oxygen ratio in the head space and the dissolved oxygen content of NGF samples tended to be lower than those of other samples over the storage period. Compared to the CON, the acid values of NGF and RHS samples were lower than the CON from month 3 and 6 (p<0.05). During storage, volatile basic nitrogen values of NGF and RHS samples increased slowly compared to the CON. To delay quality deterioration and extend the shelf-life of Samgyetang products, the reduction of oxygen content in the head space of the retort pouch (preferably by flushing with nitrogen gas) is recommended, even though no significant differences in sensory evaluation were observed among the samples during 9 months of storage (p>0.05).

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