• Journal of Food Hygiene and Safety
• /
• v.39 no.1
• /
• pp.35-43
• /
• 2024

Numerous methods have been applied to assess the antibacterial effectiveness of hand hygiene products. However, the different results obtained through various evaluation methods have complicated our understanding of the real efficacy of the products. Few studies have compared test methods for assessing the efficacy of hand hygiene products. In particular, reports on ex vivo pig skin testing are limited. This study aimed to compare and characterize the methodologies applied for evaluating hand hygiene products, involving in vitro, ex vivo, and in vivo approaches, applicable to both leave-on sanitizers and wash-off products. Our further aim was to enhance the reliability of ex vivo test protocols by identifying influential factors. We performed an in vitro method (EN1276) and an in vivo test (EN1499 and ASTM2755) with at least 20 participants, against Serratia marcescens or Escherichia coli and Staphylococcus aureus. For the ex vivo experiment, we used pig skin squares prepared in the same way as those used in the in vivo test method and determined the optimal treated sample volumes for sanitizers and the amount of water required to wash off the product. The hand sanitizers showed at least a 5-log reduction in bacterial load in the in vitro test, while they showed little antibacterial activity in the in vivo and ex vivo tests, particularly those with a low alcohol content. For the hand wash products, the in vitro test was limited because of bubble formation or the high viscosity of the products and it showed low antibacterial activity of less than a 1-log reduction against E. coli. In contrast, significantly higher log reductions were observed in ex vivo and in vivo tests, consistently demonstrating these results across the two methods. Our findings revealed that the ex vivo and in vivo tests reflect the two different antibacterial mechanisms of leave-on and wash-off products. Our proposed optimized ex vivo test was more rapid and more precise than the in vitro test to evaluate antibacterial results.

• Korean Journal of Food Science and Technology
• /
• v.45 no.2
• /
• pp.148-155
• /
• 2013

Sulfoxaflor is a new active ingredient within the sulfoximine insecticide class that acts via a unique interaction with the nicotinic receptor. The MRLs (maximun residue limit) of sulfoxaflor in apple and pear are set at 0.4 mg/kg and that in pepper is set at 0.5 mg/kg. The purpose of this study was to develop an analytical method for the determination of sulfoxaflor residues in agricultural commodities using HPLC-UVD and LC-MS. The analysis of sulfoxaflor was performed by reverse phase-HPLC using an UV detector. Acetone and methanol were used for the extraction and aminopropyl ($NH_2$) cartridge was used for the clean-up in the samples. Recovery experiments were conducted on 7 representative agricultural products to validate the analytical method. The recoveries of the proposed method ranged from 82.8% to 108.2% and relative standard deviations were less than 10%. Finally, LC-MS with selected ion monitoring was also applied to confirm the suspected residues of sulfoxaflor in agricultural commodities.

• Journal of Food Hygiene and Safety
• /
• v.27 no.3
• /
• pp.271-276
• /
• 2012

This study intented to standardize the method for total polysaccharide, which is a functional marker for aloe vera gel in Korea. We used four lyophilized raw materials and commercial aloe gel products, certified as Health Functional Food by Korea Food and Drug Administration, including powder, solution, jelly, tablet and capsule, to optimize the analytical condition of dialysis and phenol-sulfuric acid reaction in polysaccharide analysis. The optimal conditions for polysaccharide analysis included 1 L water for dialysis and change 3 times for 24hr against 25 mL prepared sample solution. Validation test showed lower than 5% of coefficient of variation(CV) in intra-, interday validation in lyophilized raw materials and 4 types of commercial products. In inter-person and inter-laboratory validation with 4 persons from 4 different laboratories, CV(%) were 5.50 and 6.64 respectively. The linearity of polysaccharide analysis was assessed using 5 serial concentration of lyophilized raw materials(0.1, 0.2, 0.3, 0.4, 0.5%(w/v)). The results showed $R^2{\geq}0.995$ of high linearity. In the commercial aloe vera gel products, the results of reproductivity showed lower than 7.08% and revealed that the standardized method from this study ensured high precision for polysaccharide analysis.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2018.06a
• /
• pp.76-76
• /
• 2018

LCD 디스플레이 크기는 점차 대형화를 이루면서, 현재 LCD 디스플레이 크기는 3,000*3,320mm 크기까지 증가하여 개발이 활발이 이루어지고 있다. 디스플레이의 크기가 증가함에 따라 제조 장비의 크기도 증가되어야 하므로, LCD 디스플레이 CVD 공정에 사용되는 4,200*3,300mm 크기의 대형 Aluminium Vacuum Chamber 에 피막두께 $15{\mu}m$ 이상을 구현함과 동시에 두께 균일도가 우수하며 염수분무시험으로 168시간 이상의 내식성 확보가 가능한 양극산화조건 개발을 위하여 양극산화 피막의 각종 특성 평가를 실시하였다. 양극산화 피막 두께 측정은 와전류(Eddy Current)의 원리를 이용한 비파괴식 두께 측정법(ISO 2360, ASTM D 7091)을 적용하였으며, 염수분무시험 방법은 (KS D 9502)을 적용하였으며, HCl bubble stream 시험 방법은 HCl 5% 농도를 투명 아크릴 튜브에 채운후 bubble stream 을 종점으로 하여 평가를 실시하였으며, 열충격을 이용한 도금밀착성(KS D 0254), 도장접착력(ASTM D 3359) 등을 이용하여 전해조건 및 전해액 농도에 따른 피막 특성 비교평가를 실시하여 최적의 대형 Aluminium Vacuum Chamber 양극산화 전해 조건을 개발하여 4,200*3,300mm 크기의 대형 Aluminium Vacuum Chamber 제조를 목적으로 하였다.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.22 no.2
• /
• pp.133-140
• /
• 2018

A nonlinear finite element analysis was conducted in order to examine the moment capacity and flexural behaviour of hollow prestressed concrete filled steel tube(HCFT) piles which compose hollow PHC piles inside thin wall steel tubes. The parameters investigated in this study were various contact conditions between concrete and steel tube, thickness of concrete tube and various PC strands. A simple method is proposed to determine the ultimate flexural strength based on plastic stress distribution method. In order to verify the proposed method, calculated moment capacity of various HCFT piles are compared with the experiment and numerical analysis results.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.32 no.4C
• /
• pp.139-147
• /
• 2012

In this study, various laboratory tests using bottom ash, which has similar engineering properties with sand, were conducted in order to solve the problem of clogging in granular compaction pile and to address sand supply and demand. In particular, testing was performed to help reduce clogging and minimize voids in a crushed stone compaction pile constructed in soft ground. Based on compaction tests and large diameter direct shear tests, an optimum mixing ratio was determined to be 80:20 (crushed stone to bottom ash) because an 80:20 mixing ratio showed the highest shear strength. Test results showed that as the bottom ash content increased above 20%, internal friction angle decreased. Another test method showed freezing and thawing had little effect when the replacement ratio was over 40%. Therefore, bottom ash mixed compaction piles in soft ground are most economical at a 40% replacement ratio.

• The Journal of Engineering Geology
• /
• v.29 no.3
• /
• pp.211-222
• /
• 2019

This study evaluates the yield load and allowable bearing capacity of ground in compressive loading tests to confirm the effect of CPR foundation reinforcement. The average compressive strength of the injection materials was higher than the planned compressive strength. Standard penetration tests for each stratum showed that foundation reinforcement improved the average N values, thereby increasing the bearing capacity of the ground. Compressive loading tests on two CPR piles revealed that the total and net settlement due to the maximum load exceed that permissible for the CPR pile diameter. The yield load and allowable bearing capacity calculated by the settlement criterion and the load-settlement curves varied greatly with the method applied. Therefore, it seems to be necessary to determine the optimum value through comprehensive analysis after applying various yield load calculation methods.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.21 no.1
• /
• pp.117-125
• /
• 2017

The role of fly ash in concrete become impotent with finding the characteristics of fly ash in which it is used as cement replacement material. In this paper, corrosion test results obtained by two test methods such as the long-term exposure corrosion test and the accelerated corrosion test method, were compared to investigated the corrosion resistance between fly ash concrete and normal concrete. Corrosion initiation time was measured in two types of concrete, i.e., one mixed with fly ash(FA) and the other without admixture(OPC). The accelerated corrosion test was carried out by four case, i.e., two samples is a cyclic drying-wetting method combined without carbonation(case 1) and combined with carbonation(case 2), and the other two samples is a artificial seawater ponding test method combined without carbonation(case 3) and combined with carbonation(case 4). Whether corrosion occurs, it was measures using half-cell potential method. The ponding test combined without carbonation was most effective in accelerating corrosion time of steel bars. The results indicated that the corrosion of rebar embedded in concrete occurred according to the order of OPC, FA. The delay relative ratio of corrosion obtained by corrosion initiation time between FA and OPC is 1.04 to 1.27. Consequently, fly ash concrete as the age increases its corrosion resistance was improved compared with OPC concrete.

• Journal of Korea Water Resources Association
• /
• v.35 no.1
• /
• pp.51-64
• /
• 2002

In this study, the formulation of the developed mixed-integer programming model for a multi-reservoir system including hydro-electric power generation (park et al., 2001) has been improved for multiple reservoir system operation using risk evaluation criteria. Sequential linear programming(SLP) was applied for the linearization of the hydro-electric energy term in the model. In order to allocate monthly reservoir release reasonably the value of weight for hydro-electric energy was assigned by level of power generation hour. The improved model was applied to the five reservoirs system in the Han river. And could be confirmed the availibility of new formulation appling risk evaluation criteria.

• Journal of Aerospace System Engineering
• /
• v.13 no.1
• /
• pp.29-37
• /
• 2019

The objective of this study was to investigate the influence of the blade shape on the impeller performance, for design optimizing of the high airflow impeller. First, the quantity, angle, and length of blades, which are considered to have a large influence on the impeller performance, were selected as design variables. Then, 27 cases of impeller shapes were selected according to the design of experiment (DOE). To predict the conduct of the blower based on the selected impeller shape, flow analysis was performed using the immersed solid method of ANSYS CFX. In the CFD results, the highest airflow was expected in the impeller having a combination of 50 EA, $6^{\circ}$ and 5 mm. Finally, a blower with the original impeller shape and the optimized impeller shape was fabricated using a 3D printer, and the analysis tendency and experimental tendency were verified through experiments.