Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Improvement of Analytical Method for Total Polysaccharides in Aloe vera Gel

알로에 베라(Aloe vera) 겔 중 총 다당체 시험법 개선

  • Lee, Young-Joo (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Yun-Je (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Leem, Dong-Gil (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Yoon, Tae-Hyung (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Shin, Ji-Eun (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Yoon, Chang-Yong (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Jung-Hoon (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Park, Mi-Sun (Department of Risk Assessment, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, Tae-Seok (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Jeong, Ja-Young (Department of Toxicological Research, National Institute of Food and Drug Safety Evaluation)
  • 이영주 (식품의약품안전평가원 영양기능연구팀) ;
  • 김윤제 (식품의약품안전평가원 영양기능연구팀) ;
  • 임동길 (식품의약품안전평가원 영양기능연구팀) ;
  • 윤태형 (식품의약품안전평가원 영양기능연구팀) ;
  • 신지은 (식품의약품안전평가원 영양기능연구팀) ;
  • 윤창용 (식품의약품안전평가원 영양기능연구팀) ;
  • 김정훈 (식품의약품안전평가원 영양기능연구팀) ;
  • 박미선 (식품의약품안전평가원 위해분석연구과) ;
  • 강태석 (식품의약품안전평가원 영양기능연구팀) ;
  • 정자영 (식품의약품안전평가원 독성연구과)
  • Received : 2012.02.28
  • Accepted : 2012.07.18
  • Published : 2012.09.30
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Abstract

This study intented to standardize the method for total polysaccharide, which is a functional marker for aloe vera gel in Korea. We used four lyophilized raw materials and commercial aloe gel products, certified as Health Functional Food by Korea Food and Drug Administration, including powder, solution, jelly, tablet and capsule, to optimize the analytical condition of dialysis and phenol-sulfuric acid reaction in polysaccharide analysis. The optimal conditions for polysaccharide analysis included 1 L water for dialysis and change 3 times for 24hr against 25 mL prepared sample solution. Validation test showed lower than 5% of coefficient of variation(CV) in intra-, interday validation in lyophilized raw materials and 4 types of commercial products. In inter-person and inter-laboratory validation with 4 persons from 4 different laboratories, CV(%) were 5.50 and 6.64 respectively. The linearity of polysaccharide analysis was assessed using 5 serial concentration of lyophilized raw materials(0.1, 0.2, 0.3, 0.4, 0.5%(w/v)). The results showed $R^2{\geq}0.995$ of high linearity. In the commercial aloe vera gel products, the results of reproductivity showed lower than 7.08% and revealed that the standardized method from this study ensured high precision for polysaccharide analysis.

건강기능식품 중 알로에 베라 겔 제품의 기능성분(지표성분)은 총 다당체로 설정되어 있으며 식품공전 시험법은 페놀황산법에 의하여 총 다당체 함량을 측정하도록 되어 있다. 본 연구에서는 페놀황산 반응에 의한 총 다당체 분석의 분석편차 발생의 주요인으로 파악된 투석단계에 대하여 세부 분석조건(투석막의 너비, 투석외액의 부피와 교체시간, 투석 후 정용여부, 페놀황산 반응시간)에 대한 최적 조건을 설정하였고 이를 토대로 표준화된 시험법을 마련하여 실험자, 실험실의 주관적 설정에 의한 편차발생을 최소화하고자 하였다. 표준화 된 시험법의 검증을 위해 알로에 베라 겔 원료성 분말 및 제품에 대하여 일내(2.56% 이하), 일간(4.85% 이하) 정밀성과 실험실간(5.50% 이하), 실험자간(6.64% 이하) 정밀성을 확인한 결과, 비교적 높은 정밀성을 확보할 수 있었다. 알로에 겔 유통제품에 대한 정밀성에서도 비교적 신뢰할 수 있는 수준(7.08% 이하)의 결과를 확인하였다. 최적화한 표준 시험법에 따른 총 다당체 분석은 알로에 겔 제품 검사의 정밀성을 확보할 수 있을 것으로 평가되었다.

Keywords

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