• Journal of Environmental Science International
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• v.24 no.6
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• pp.763-767
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• 2015

The present study was conducted to development mass production methods for peanut sprouts that is considered as a field of blue ocean among the agricultural products. 'Jopyeong' was the best as a major cultivar for peanut sprouts production. The manual for the production of high-quality peanut sprouts is as following. Germination temperature appropriate for production of high-quality peanut sprouts was $27^{\circ}C$. Peanut sprouts at the growth stage of 8th day, and older plants with advanced growth showed deteriorated merchantable and eating quality. Resveratrol compound was not found in the seeds, but its highest amount was detected from 9-day old sprouts. The best water temperature applicable to high quality peanut sprout production was $25^{\circ}C$. The growth of peanut sprout was inhibited by the high temperatures above $35^{\circ}C$ and low temperatures below $15^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.1
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• pp.48-54
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• 2019

This research was carried out to investigate the plant growth, inorganic ion and amino acid content characteristics in a halophyte, Suaeda glauca, under different NaCl concentrations for cultivating in the reclaimed land. S. glauca was hydroponically cultivated under 0, 50, 100, 200, 300 and 400 mM NaCl concentrations with Hogland's nutrient solution. To evaluate growth response under different NaCl concentrations, plant height and number of branches, dry weight, Fv/Fm value, and photosynthetic efficiency were investigated. To find out physiological characteristic, inorganic ion contents and amino acids in the plant were evaluated. The optimum concentration of NaCl for plant growth were 50 mM. The plant growth were gradually decreased in the concentration ranged from 100 to 400 mM. As increasing of NaCl concentration, Na ion was increased, but K, Ca, Mg ions were decreased in the plant. The amino acid contents were varied due to NaCl concentrations, but most of amino acids content in total plant was the lowest at 50 mM. Conversely, proline was exceptionally high at 50 mM of NaCl concentration. The Fv/Fm value was the highest at 50 mM of NaCl concentration. From these results, the optimum salt concentration for the growth of S. glauca was 50 mM, but the plant seems to adapt in a variety of salt environments in view of the change of ions and amino acids depending on salt concentration and the maintenance of photosynthetic efficiency even under high salt condition.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.34-34
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• 2019

털부처꽃(Lythrum salicaria L.)은 7~8월에 최산꽃차례로 붉은 자주색의 꽃이 피며, 밀원식물로 이용된다. 또한 예로부터 발효해서 술로 마시고 잎은 채소로 식용하였으며, 식중독 치료 및 설사를 멈추게 하는 효과가 있는 것으로 알려진 식물이다. 한국, 중국, 아프리카, 유럽, 북아메리카 등의 습한 지역에 분포한다. 본 연구는 다방면으로 이용이 가능한 털부처꽃을 관상용 소재로 개발하기 위한 최적의 유묘 재배 기술을 확립하기 위해 수행되었다. 유리온실 내에서 2019년 4월 27일에 종자를 파종하여 8주간 육묘하였다. 파종 용기는 162, 200 및 288 트레이로 달리하였으며, 토양 실험은 원예상토와 피트모스:펄라이트 3:1, 4:1 혼용토를 사용하였다. 파종립수 실험은 200구 트레이에 셀 당 1, 2, 4 및 6립으로 달리하였고, 추비는 hyponex (6.5-6-19)를 0, 250, 500, $1000mg{\cdot}L^{-1}$의 농도로 출아 후 2주 간격으로 총 3회 엽면시비하였다. 또한 차광 실험은 0, 55, 75%로 설정하였다. 대조구는 200구 트레이에 원예상토를 충진하고 셀당 1립씩 파종하여 무차광 조건에서 육묘하였으며, hyponex $500mg{\cdot}L^{-1}$를 추비하였다. 연구의 결과, 파종용기에 따른 털부처꽃 유묘는 셀 당 토양의 양이 가장 많은 162구 트레이에서 왕성하였다. 토양 종류별 실험은 원예상토 단용구에서 모든 생육이 양호한 결과를 보였으며, 피트모스와 펄라이트의 혼용토에서는 생육이 억제되었다. 파종립수에 따른 초장의 유의적인 생육 차이는 없었으나, 전반적인 생육은 1립 파종에서 우수하였다. 한편 2립 또는 4립을 파종구에서 비교적 생육이 양호하고 풍성하게 보이는 효과가 있어 관상적 이용에 적합할 것으로 생각되었다. 추비처리는 hyponex의 농도가 가장 높은 $1000mg{\cdot}L^{-1}$ 처리구에서 유묘의 생육이 가장 양호하였고, $500mg{\cdot}L^{-1}$ 처리에서는 무처리와 유의적인 차이가 없었다. 차광처리는 털부처꽃의 유묘 재배시 전반적인 생육을 증가시켰으며, 55% 차광에서 가장 우수하였다. 결론적으로 털부처꽃의 육묘는 162구 트레이에 원예상토를 충진한 다음 셀 당 2립 또는 4립을 파종하고 55% 차광 조건에서 재배하는 것이 효과적이며, 추비는 hyponex $1000mg{\cdot}L^{-1}$를 처리하는 것이 양묘 생산에 적합한 것으로 판단된다.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.374-383
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• 2010

Microwave radar can penetrate cloud cover regardless of weather conditions and can be used day and night. Especially a ground-based polarimetric scatterometer has advantages of monitoring crop conditions continuously with full polarization and different frequencies. Kim et al. (2009) have measured backscattering coefficients of paddy rice using L-, C-, X-band scatterometer system with full polarization and various angles during the rice growth period and have revealed the necessity of near-continuous automatic measurement to eliminate the difficulties, inaccuracy and sparseness of data acquisitions arising from manual operation of the system. In this study, we constructed an X-band automatic scatterometer system, analyzed scattering characteristics of paddy rice from X-band scatterometer data and estimated rice growth parameter using backscattering coefficients in X-band. The system was installed inside a shelter in an experimental paddy field at the National Academy of Agricultural Science (NAAS) before rice transplanting. The scatterometer system consists of X-band antennas, HP8720D vector network analyzer, RF cables and personal computer that controls frequency, polarization and data storage. This system using automatically measures fully-polarimetric backscattering coefficients of rice crop every 10 minutes. The backscattering coefficients were calculated from the measured data at a fixed incidence angle of $45^{\circ}$ and with full polarization (HH, VV, HV, VH) by applying the radar equation and compared with rice growth data such as plant height, stem number, fresh dry weight and Leaf Area Index (LAI) that were collected at the same time of each rice growth parameter. We examined the temporal behaviour of the backscattering coefficients of the rice crop at X-band during rice growth period. The HH-, VV-polarization backscattering coefficients steadily increased toward panicle initiation stage, thereafter decreased and again increased in early-September. We analyzed the relationships between backscattering coefficients in X-band and plant parameters and predicted the rice growth parameters using backscattering coefficients. It was confirmed that X-band is sensitive to grain maturity at near harvesting season.

• Journal of Life Science
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• v.18 no.12
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• pp.1625-1630
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• 2008

A microorganism capable of producing high level of poly-3-hydoxybutyrate (PHB) from xylose was isolated from soil. The isolated strain J-65 was identified as Bacillus megaterium based on the morphological, biochemical and molecular biological characteristics. The optimum temperature and pH for the growth of B. megaterium J-65 were $37^{\circ}C$ and 8.0, respectively. The optimum medium composition for the cell growth was 2% xylose, 0.25% $(NH_4)_2SO_4$, 0.3% $Na_2HPO_4{\cdot}12H_2O$, and 0.1% $KH_2PO_4$. The optimum condition for PHB accumulation was same to the optimum condition for cell growth. Copolymer of ${\beta}$-hydroxybutyric and ${\beta}$-hydroxyvaleric acid was produced when propionic acid was added to shake flasks containing 20 g/l of xylose. Fermenter culture was carried out to produce the high concentration of PHB. In batch culture, cell mass was 9.82 g/l and PHB content was 35% of dry cell weight. PHB produced by B. megaterium J-65 was identified as homopolymer of 3-hydoxybutyric acid by GC and NMR.

• Applied Biological Chemistry
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• v.40 no.1
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• pp.18-22
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• 1997

The conditions for production of high cell density of Bifidobacterium longum were investigated and the cross-flow filtration system was used to remove the inhibitory metabolites, lactic acid and acetic acid. The maximum cell growth was observed with glucose as carbon source at the concentration of 50 g/l at $37^{\circ}C$ with the initial pH 6.5. When B. longum was cultured in a cross-flow filtration system, the maximum cell growth was observed at a dilution rate(D) of $0.31\;h^{-1}$ and the dry cell weight was 16.4 g/l($3.5{\times}10^{10}\;cell/ml$), which was about four times higher than that obtained in the batch culture with pH control.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.524-528
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• 1989

A $\beta$-Galactosidase producing strain, Alkalophilic Bacillus sp, YS-309, has been isolated from soil sample. The strain was capable of producing large amount of intracellular $\beta$-galactosidase in the alkaline media rather than in the neutral media. The preferable medium composition has been determined to be as follows: 0.5% lactose, 0.5% yeast extract, 0.5% soybean meal, 0.1% KH$_2$PO$_4$, 0.02% MgSO$_4$7$H_2O$ 0,0.6% Na$_2$CO$_3$ (pH 9.9). The enzyme was produced by lactose or IPTG as in-ducer. But both Enzyme synthesis and cellular growth were decreased when lactose was added at the higher concentrations than 1.5% (v/v).

• Journal of Ginseng Research
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• v.11 no.1
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• pp.56-65
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• 1987

Grown-gall tumor was induced from the infection of Panax ginseng C.A. Meyer by Agrobacterium tumefaciens C58 and the tumor calli were formed on the phytohormone free MS medium. The calli were friable and rough in appearance. Calli obtained from crown gall tumor were similar to and indistinguishable from each other. The tumor callus was quite different from normal callus. Tumor callus grew rapidly, whereas mal callus appeared late. The growth of tumor callus was better in the dark than in the light. In suspension culture, the fresh weight of tumor callus was twice as much in comparison with normal callus.

• Journal of Life Science
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• v.18 no.1
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• pp.103-108
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• 2008

An agar-degrading marine bacterium, strain AS-1 was isolated from the seawater. The strain AS-1 was identified as Sphingomonas paucimobilis (90% probability) by VITEK. The optimum medium for agarase activity of the isolated strain was determined to be marine medium, marine broth 2216 containing 0.1% agar as carbon source. An extracellular agarase was purified 104-fold from the culture supernatant by ammonium sulfate precipitation, ion exchange chromatography and gel filtration methods. The molecular weight of the purified enzyme was estimated to be 80 kDa by SDS-PAGE. The optimum pH and temperature for activity were 7.0 and $40^{\circ}C$, respectively. Antioxidative activity of the strain AS- was 72% in the supernatant cultured for 12 h. The culture supernatant of the strain AS-1 showed antibacterial activity against bacteria causing putrefaction and food poisoning such as Escherichia coli, Staphylococcus aureus and Proteus vulgaris. However, the cell growth of the lactic aicd forming strain, Lactobacillus plantarium was promoted by the treatment of 10% culture supernatant of an agar-degrading strain.

• Korean Journal of Plant Resources
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• v.30 no.1
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• pp.50-57
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• 2017

This study aimed to investigate the morphological characteristics and optimal germination conditions in seeds of Arabis pendula L., a traditionally edible and medicinal plant. The external seed shape was circular-obovate with narrow wings and dark brown. The seed length and width were 1.25 mm and 1.47 mm, respectively. The seeds were exalbuminous and the embryo was a bent type. Seed germination was the highest (49.7%) at $20^{\circ}C$ under dark conditions among the various temperature and light conditions applied. However, under the dark condition, the seedling was weak, overgrown, and the cotyledons were small and folded. To improve the germination and growth of seedlings, the seeds were pre-treated with $GA_3$ solutions of varying concentrations (0, 200, 500, and 1,000 mg/L). The seed germination and seedling growth were effectively improved by $GA_3$ pre-treatment. The germination rate was the highest (97.3%), mean germination time was the shortest (8.1 days), and a vigorous growth of seedlings was observed upon pre-soaking the seeds in 500 mg/L $GA_3$ solution. In conclusion, the best method for germination was pre-soaking in 500 mg/L $GA_3$ ($4^{\circ}C$, dark, 24 h) and incubating the seeds at $20^{\circ}C$ for 15 days.