• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.3 no.1
• /
• pp.1-6
• /
• 1970

A series of experiments have been made on the mass culture of Phaeodactylum tricornutum, Platymonas sp. and Chlorella sp. in the laboratory. The shortest lag phase was found In the culture of Ph. tricornutum followed by Platymonas sp. and Chlorella sp. As compared to the aeration culture, the stagnant culture, in general, showed long duration of the lag phase, short period of the exponential phase and extremely small daily increment. The relative growth constants of Ph. tricornutum, Chlorella sp. and Platymonas sp. were $0.302{\pm}0.028$, $0.226{\pm}0.013$, and $0.151{\pm}0.008$, respectively The maximum daily increment of the three species and then daily ages are as follows: Ph. tricornutum Maximum daily increment : 47.5, Daily age : 10, Platymonas sp. Maximum daily increment : 5.6, Daily age : 14, Chlorella sp. Maximum daily increment : 21.1, Daily age : 14 Comparing the packed cell volume with a certain number of cells, the largest value was found in the population of Ph. tricornutum followed by Platymenas sp. and Chlorella sp. A straight line relationship exists between the two values, and the magnitude of the relationship coincides well with the size of the cells. The culture of Ph. tricornutum was proved satisfactory for feeding the larvae of bivalves at about 12 days after innoculation and both of Platymenas sp. and Chlorella sp. were about 16 days respectively.

• Journal of Aquaculture
• /
• v.21 no.4
• /
• pp.331-338
• /
• 2008

Monthly changes in the gonadosomatic index (GSI) and hepatosomatic index (HSI) of wild river puffer Takifugu obscurus, and water quality environment in spawning area during breeding season were investigated from March 1995 to February 1996. Monthly changes in GSI and HSI of T. obscurus, that was cultured in low salinity, were calculated. The external morphology of the gonads, germ cell differentiation during gametogenesis and the reproductive cycle with the gonad developmental phases were investigated by histological analysis. The optimum water quality environment in Ganggyung, Choongcheongnam-do, where is spawning ground of wild T. obscurus, was $15-20^{\circ}C$ (water temperature) and 0 psu (salinity). Monthly changes in the GSI in females and males reached a maximum in May, and then rapidly decreased. Therefore, it is assumed that in the natural condition the spawning period of wild T. obscurus is May to June. In females and males, it showed a negative correlationship between the GSI and HSI. The external morphology of the gonads in female and male T. obscurus, that was cultured in low salinity, is composed of a pair of saccular structure. Based on monthly changes in the GSI, it is assumed that in female T. obscurus, that was cultured in low salinity, spawn from March through May. Therefore, it showed a negative correlationship between changes in the GSI and HSI. On the whole, in females and males, it showed a similar pattern between wild and cultured T. obscurus. The reproductive cycle with the gonad developmental phases can be classified into successive five stages in females: the early growing stage, late growing stage, mature stage, ripe and spent stage, and recovery and resting stage. In males, that can be divided into successive four stages: the growing stage, mature stage, ripe and spent stage, and recovery and resting stage. In case of wild T. obscurus, the spawning period has once a year, however, those cultured in the high water temperature ($20-27^{\circ}C$) - low salinity (under 3.3 psu) condition have reproductive characteristics having possibilities of discharge of eggs and sperms year-round as a multiple spawner.

• Development and Reproduction
• /
• v.16 no.2
• /
• pp.145-153
• /
• 2012

Reproductive cycle of the small abalone, Haliotis diversicolor aquatilis which collected from Seong-san coastal waters, Jeju, Korea were investigated monthly from May 2006 to April 2007 using histological methods. The gonad index (GI) of male and female reached a peak in June ($70.72{\pm}5.20$) and July ($55.38{\pm}11.73$). Subsequently, GI decreased in September (Male, $21.27{\pm}2.91$; Female, $27.75{\pm}4.76$) and increased again in October (Male, $48.49{\pm}8.39$; Female, $51.36{\pm}7.47$), respectively. After that, GI gradually decreased. In March 2007, GI was reached the minimum (Male, $8.46{\pm}0.57$; Female, $9.69{\pm}0.88$). The reproductive cycle of female and male could be divided into six successive stages ; In female, multiplication (February and May), growing (February to May), mature (April to July), partial spawning (May to November), degenerating (October to December) and recovery (September to October and December to February) stage. In male, multiplication (February and May), growing (March to May), mature (April to July), spawning (June to November), degenerating (November to February) and recovery (September to February) stage. The yellowish granular cells have been observed more in multiplication, growing, degenerating, and recovery stage than mature and partial spawning stage. The results of GI and histological observations of the gonads suggested that this species could be presumed as multi- spawning characteristics more two times in spawning seasons.

• Development and Reproduction
• /
• v.10 no.1
• /
• pp.33-39
• /
• 2006

Gonadal development and reproductive cycle of the North Pacific seastar, Asterias amurensis captured from the Gosung, Gyeongsangnamdo, between November 2003 and February 2005, was investigated monthly changes of gonadosomatic index(GSI), gonadal development and oocyte size-frequency distribution. Monthly changes of GSI values showed similar trends in female and male. GSI values were reached the maximum in March($3.88{\pm}3.04$ in female, $0.87{\pm}0.57$ in male), and then gradually decreased. Based on the monthly changes of GSI, histological observation of gonadal development, reproductive cycle was divided into following successive stage: growing stage(October to January), mature stage(February to March), spent stage(March to April), degeneration and resorption stage(April to May), recovery stage(July to September). based on these result, this species seemed to have a synchronous oocyte development and one spawning season a year.

• Korean Journal of Ichthyology
• /
• v.7 no.1
• /
• pp.33-42
• /
• 1995

The reproductive cycle of the bitterling, Rhodeus uyekii was studied to observe the annual variations of gonadosomatic index(GSI), size frequency distribution of egg, ovipositor length and histological changes of gonad. GSI began to increase from February when the water temperature started to increase, and reached the maximum value in May, whereas it began to decrease from July and reached the minimum value in August which in the highest water temperature season. It began to incerase again but showed low value from September to November. The GSI remained stable thereafter. Monthly changes in GSI, ovipositor length, frequency of egg diameter and gonadal histology showed that the annual reproductive cycle was classified into the following successive phases : primary growing phase from September to November, quiescent phase in December, secondary growing and mature phase from January to February, ripe and spawning phase from March to June, and recovery and resting phase from July to August.

• KSBB Journal
• /
• v.10 no.2
• /
• pp.196-203
• /
• 1995

The effects of majors nutrients and environmental factors on polyoxins biosysnthesis were examined for the overprodution of antifungal agent polyoxins using Streptomyces sp. 809-11. The cell mass at the exponential growth phase was increased when soluble starch was used, while the polyoxins biosynthesis was increased with the formation of filamentous mycelium when glucose was used as a carbon source. It was, therefore, recommendable to use both soluble starch and glucose simultaneously as carbon sources for the cell growth and polyoxins production. The high concentration of ammonium sulfate (151.4 mM) resulied in the improvement of polyoxins production. The optimal concentration of $K_2HPO_4$for polyoxins production was found to be 0.5mM. By applying fed-batch culture, polyoxins production was improved to about 2-folds compared to the result from the batch culture.

• Journal of Aquaculture
• /
• v.2 no.1
• /
• pp.43-51
• /
• 1989

Fatty acid contents were measured in the cultures of the flagellate green algae Dunaliella tertiolecta Butcher under different conditions of light intensity, duration of light, and temperature. Duration of light and temperature, in particular, affected the growth rate of D. tertiolecta. The maximun cell number reached $2.32{\times}10^6$ cells/ml. The division rate per day was 1.97 in the exponential phase. The analysis of fatty acids obtained from various conditions showed that the lipid mainly contained C16, C18:3$\omega$3 fatty acids and there was no significant level of polyunsaturated fatty acids such as EPA and DHA. Polyene fatty acid increased with decreasing temperature and light intensity did not influence on fatty acid composition. The increasing duration of light enhanced the growth of D. tertiolecta, whereas polyene($\omega$3) slightly increased with decreasing the light period.

• KSBB Journal
• /
• v.9 no.3
• /
• pp.287-293
• /
• 1994

A bacterium isolated from waste coal mine water around Hawsun had an ability for the degradation of hydrogen sulfide. The isolate was identified as Thiobacillus sp. IW. on the basis of its morphological, physiological and chemotaxonomical characteristics. The optimum pH and temperature were 7 and $30^{\circ}C$, respectively. Growth occurred in a pH range of 3 to 9. Due to the sulfate accumulated in liquid medium, the pH decreased. As a consequence the cell growth was inhibited. Potasium nitrate and glutamic acid were utilized as a nitrogen source but urea and ammonium chloride not consumed. Denitrification occurred in a basal medium containing the glucose but did not in a basal medium containing the malate. The maximum specific growth rate of cell was 0.78h-1 and generation time was 0.9 hour. The cell productivity was 6.25mg/1$.$h and the isolate grew logarithmically up to 18 hour. These results indicate that the isolate can be a suitable bacterium responsible for degradation of hydrogen sulfide as malodorous compounds.

• Korean Journal of Plant Resources
• /
• v.30 no.4
• /
• pp.466-474
• /
• 2017

The Omija (Schizandra chinensis Baillon) is a fruit native to northeast Asia that is cultivated in South Korea and China. Using 1,938 valid patents of 6 group countries, we analyzed the patent trend based on year, countries, applicants, and technology. The technologies are categorized the 10 sub-technologies such as medicine, quasi-drugs, food, feed, cosmetics, cultivation, genome, manufacture, preprocessing, and etc. The technology level and competitiveness are analyzed using patent index such as cites per patent, patent impact index, patent family size and technology strength. In Korea, patent number rapidly increasing and individual technical level is lower than other countries. However, overall technical competitiveness is estimated high due to multiple patents. We suggest that cosmetics and cultivation fields are most likely to be developed in future omiza technology development in Korea. Our study will provides to the information of technical trend to support performing of new projects for Omija plant.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2018.05a
• /
• pp.494-494
• /
• 2018

최근 하천에서 홍수방지를 위한 제방과 댐 같은 구조물이 지속적으로 건설되고 있으며 이러한 구조물의 설치는 자연적인 하천의 발달과정을 느리게 하고 어류의 서식처를 감소시킨다. 특히 주기적인 홍수 및 홍수터를 필요로 하는 어류는 유량의 규제 및 홍수터의 감소로 심각한 영향을 받게 되었고, 많은 종류의 어류가 환경 및 생태계의 변화로 멸종했다. 따라서 수중생태계에서 어류의 서식환경을 보호하고 유지하기 위해서는 공간확대를 통한 적절한 서식처가 보장되어야 한다. 본 연구에서는 공간복원 시나리오에 따른 수환경 변화가 어류 물리서식처에 미치는 영향을 분석하기 위해 2차원 물리서식처 모형인 River2D를 적용하였다. 만경강 유역의 봉동, 소양, 하리 3개의 지점을 경계지점으로 하여 대상영역 내 저지대 상습 침수구역인 만경강 본류와 소양천 합류지점을 복원대상지로 선정 후, 현재하도와 세가지 복원 시나리오(제방 셋백, 구하도, 배후습지)를 적용하여 분석을 실시하였다. 이 분석에서 모니터링 된 과거 흐름과 세가지 물리적 복원 시나리오 뿐만 아니라 필요한 유량조건의 범위에 있는 목표종 성장단계는 홍수터 서식지 이점에 영향을 미친다. 분석결과 홍수터 지역의 물리적 복원을 통해 대상어종의 생태계 복원 전의 부정적인 영향을 줄일 수 있었고 특히 WUA 분석에 따르면 세가지 복원 시나리오 적용시 복원전보다 서식처 개선효과가 크게 나타나는 것을 확인할 수 있었다. 구하도 복원의 경우 WUA 값은 산란기에 20.0%, 성장기에 39.5%로 가장 큰 효과를 나타냈고, 그 결과와는 다르게 배후습지 복원의 경우 성어기에 85.3%로 가장 크게 나타났다. 또한 동일한 흐름에서 홍수터의 확보 및 복원을 통해 최소유량에서 최대서식처 개선효과를 얻을 수 있음을 확인하였다. 최종적으로 복원이 성공적으로 이루어지기 위해서는 복원계획에서 대상어종의 성장단계와 복원 시나리오를 고려해야 하고 정확하게 검토해야 하며, 생태학적 특성도 면밀히 조사되어야 할 것으로 사료된다. 또한 앞서 분석된 데이터는 최적의 하천 복원 유형을 판단하기 위한 기초자료 및 판단근거로 활용될 수 있을 것으로 기대된다.