• Journal of Life Science
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• v.19 no.1
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• pp.15-20
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• 2009

Uncoupling protein 3 (UCP3) is a mitochondrial protein that is expressed predominantly in skeletal muscle. It may play a role in altering metabolic function. However, its major physiological roles are not fully understood. Recently de novo expression of UCP3 in rat liver by fenofibrate was reported. We also reported previously that fenofibrate-induced de novo expression of UCP3 contributes to reduction of adipose tissue in obese rats. In the present study, we investigated that ienofibrate-induced expression of UCP3 in rat liver is related with metabolic function such as body weight and hepatic lipid content by time-dependent manner in high-fat diet rats. Eight-week-old male Sprague-Dawley rats were randomly divided into two groups; the high fat diet group (HF, n=16) and fenofibrate-treated high fat diet group (HFF, n=16). The mRNA expression of hepatic UCP3 was detected as early as 1 week of fenofibrate treatment by quantitative real-time PCR and the amount of mRNA was increased time-dependently. The mean body weight of the HFF group was significantly less com. pared with the HF group after 6 weeks of fenofibrate treatment, even though there was no difference of food intake between the two groups. Rectal temperature was increased during 4 to 6 weeks of fenofibrate treatment and body weight was decreased after 6 weeks of treatment. These results were corresponded with the increased amount of the expression of UCP3 mRNA and protein. We suggest that de novo expression of hepatic UCP3 is increased time-dependently with fenofibrate treatment and that the amount of expression is correlated with metabolic function.

• Journal of Embryo Transfer
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• v.16 no.2
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• pp.107-115
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• 2001

Development of effective activation protocols is of great importance for improving the success of cloning and subsequent transgenic. Three methods for oocyte activation, including 5$\mu$M ionomycin (5 min) alone, ionomycin + 1.9 mM 6-dimetylaminopurine (DMAP, 3 hrs) and ionomycin + 10 $\mu\textrm{g}$/ml cycloheximide (CHX, 3 hrs) were compared for their effects of pronuclei (PN) formation, development, developmental velocity and ploidy of parthenotes to IVF control in bovine. In group of ionomycin + DMAP, the oocytes having more 3PN were significantly (P<0.05) higher than in groups of ionomycin alone and of ionomycin + CHX (45.5% vs. 0 and 0%, respectively). Activation with the ionomycin alone, ionomycin + DMAP and ionomycin + CHX resulted in cleavage rates of 30, 85.5 and 57.9%, respectively. The blastocysts rate of parthenotes activated by ionomycin + DMAP treatment was significantly higher (12.3%. p<0.05) than those of other treated groups. Chromosome analysis shows that ionomycin + DMAP treatment greatly enhances the incidence of chromosomal abnormality of the parthenotes. From the results, we may conclude that DMAP treatment to the oocytes accelerates developmental velocity resulting in both the higher incidence of chromosome abnormality and of PN formation, and strongly suggest that CHX combined with ionomycin is better than DMAP for the purpose of successful nuclear transplantation. Developmental velocity of parthenotes activated by ionomycin + DMAP treatment was significantly (P<0.05) faster than others.

• Korean Journal of Food Science and Technology
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• v.25 no.5
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• pp.449-455
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• 1993

Vibrio vulnificus is a recentry recognized halophilic organism that may cause human infections. Patients infected with Vibrio vulnificus often have a history of exposure to the sea, suggesting that the organism may be common inhabitant of marine environment. In this studies 314 samples were collected from 9 sites of Chonnam coastal area, from April to Jun, 1993. Isolation rates of V. vulnificus were 7.1% in total samples, 33.3% in sea water, 55.5% in sediment, 1.7% in fish and shellfish, 6.3% in kitchen environment. In each areas of Chunnam V. vunificus were isolated from 3.6% to 11.4%. The isolation rates of V. vulnificus was correlated positively with organic matter and COD in sea warer and sediment. In sea water, Microorganism population were $4.5{\times}10^3{\sim}3.5{\times}10^5\;CFU/ml$, Vibrio strains were $1.8{\times}10^l4.6{\times}10^3\;CFU/ml$, Coliform bacteria were $1.9{\times}10^1{\times}3.7{\times}10^2\;CFU/100ml$. In sediment, Microorganism population were $4.8{\times}10^3{\sim}5.2{\times}10^5\;CFU/ml$. Vibrio strains were $1.9{\times}10^2{\sim}8.4{\times}10^4\;CFU/ml$, Coliform bacteria were $7.2{\times}10^1{\sim}9.9{\times}10^2\;CFU/100ml$.

• Childhood Kidney Diseases
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• v.8 no.1
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• pp.26-32
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• 2004

Purpose : Hypercoagulability is present in patients with nephrotic syndrome. Plasminogen activator inhibitor type 1(PAI-1) is a major inhibitor of plasminogen activators. PAI-1 inactivates both tissue plasminogen activator(tPA) and urokinase plasminogen activator(uPA) by rapid formation of inactive 1:1 stoichiometric complexes. Recently some studies showed that the enhanced PAI-1 expression may be involved in the intraglomerular fibrinogen/fibrinrelated antigen deposition seen in nephrotic syndrome. Methods : PAI-1 gene promoter -844(G/A) polymorphism was evaluated in 146 children with minimal change nephrotic syndrome(MCNS) and 230 control subjects. The patients with MCNS were subdivided into 85 infrequent-relapser(IR) group and 61 frequent relapser(FR) group. PCR of PAI-1 gene promoter region including -844(G/A) and RFLP using the restriction enzyme Xhol were performed for each DNA samples extracted from the groups. Results : The distribution of PAI-1 genotype in the control group was G/G 81(32.5%), A/A 42(16.9%), and G/A 126(50.6%). The distribution of PAI-1 genotypes in the IR group of MCNS was G/G 29(34.1%), A/A 15(17.7%), and G/A 41(48.2%). The distribution of PAI-1 genotype in the FR group of MCNS was G/G 17(27.9%), A/A 18(29.5%), and G/A 26(42.6%). There was a significantly increased frequency of A/A genotype(P=0.0251) in the FR group of MCNS. Conclusion : Our results indicate that the PAI-1 gene promoter A/A genotype may be associated with the FR in MCNS.

• Korean Journal of Soil Science and Fertilizer
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• v.24
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• pp.5-38
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• 1991

비료(肥料)를 공업적(工業的)으로 제조(製造)하여 농업(農業)에 사용(使用)하기 시작(始作)한 이래(以來) 신비종(新肥種)의 개발(開發)에 대한 관심(關心)은 부단(不斷)히 계속(繼續)되어 왔으며 현재(現在)에도 어느 국가(國家)나 생산업계(生産業界)에서 개발(開發)을 위한 노력(努力)을 많이 기우리고 있음이 현실(現實)일 것이다. 어느 국가(國家)와 어느 시대(時代)에 있어서나 현존(現存) 비종(肥種)을 생산(生産)함에는 소비(消費)와 생산면(生産面)에서 상응(相應)하는 사정(事情)이 있었을 것이며 또한 미래(未來)에도 신비종(新肥種)을 개량(改良)하거나 창제(創製)함에는 여러 가지 관련문제(關聯問題)를 검토(檢討)하여 새로운 요구(要求)에 합리적(合理的)으로 부합(符合)되도록 설계(設計)해야 할 것이다. 비료(肥料)는 현대(現代) 농업(農業)에서 불가피(不可避)하게 사용(使用)되는 기본자재(基本資材)이며 세계적(世界的)으로 그 소비(消費)가 증가(增加)되나 국가별(國家別) 농업(農業)의 특수성(特殊性)에 맞추어서 비료(肥料)의 형태(形態)와 생산량(生産量)에 차이(差異)가 있게 되며 또 변화(變化), 개량(改良)될 것이다. 우리나라의 농업(農業)이 최근(最近) 급속(急速)히 변화(變化)되면서 생산수단(生産手段)과 경영(經營)에도 큰 변혁(變革)이 불가피(不可避)하게 따라야 할 것으로 판단(判斷)된다. 생산수단(生産手段)의 개량(改良)은 농산물(農産物) 생산가(生産價)를 낮추고 노력(勞力)의 투입(投入)을 줄이며 우수품질(優秀品質)의 농산물(農産物)을 안정적(安定的)으로 생산(生産)하기 위한 대응책(對應策)이 될 것이다. 비료(肥料)의 사용(使用)은 이러한 시대적(時代的) 요구(要求)에 맞추어 신비종(新肥種)이 개발(開發)되고 시용기술면(施用技術面)에서 발전(發展)이 있어야 될 것이다. 미래(未來)의 우리 나라 농업(農業)을 위하여 개발(開發)되어야 할 비종(肥種)의 형태(形態)와 그에 관련(關聯)되는 요건(要件)은 다음과 같이 요약(要約)된다. 1. 시비효율(施肥效率)을 높히기 위하여서나 성력재배(省力裁培)를 위하여 완효성(緩效性) 비료(肥料) 특히 질소비료(窒素肥料)의 신비종(新肥種) 개발(開發)이 절실(切實)히 필요(必要)하다고 판단(判斷)됨. 신비종(新肥種)은 대상작물(對象作物)의 종류(種類), 재배방식(裁培方式) 및 기계화(機械化) 상태(狀態)에 적합(適合)한 형태별(形態別)로 제조(製造)됨이 바람직함. 완효성(緩效性) 질소비료(窒素肥料)의 제조(製造)에는 화학합성(化學合成), 성형화(成形化), 흡착화(吸着化) 및 피복화(被覆化) 방식중(方式中) 성형화(成形化)에 의한 것이 최다(最多)이나 피복화(被覆化)도 재료(材料)와 기술(技術)의 발전(發展)에 따라 증가(增加)의 경향(傾向)임. 2. 현존(現存) 복합비료(複合肥料)의 토양(土壤) 및 작물(作物)에 대한 적합성(適合性)을 재검토(再檢討)하여 효율(效率)을 높히도록 개량(改良)함. 3. 소시비방식(少施肥方式)의 영농(營農)으로서 환경(環境)을 오염(汚染)으로부터 방호(防護)할 것이며 4. 작물종류(作物種類)(품종포함(品種包含)) 유전자형별(遺傳子型別) 비료감응성(肥料感應性)을 검정(檢定)하여 소시비(少施肥), 고효율(高效率)을 기(期)하도록함. 5 작물(作物)의 영양생리학(營養生理學) 분야(分野)에서도 비료외적(肥料外的)인 성장관련요인(成長關聯要因)의 작용(作用)을 연구(硏究)하여 응용(應用)의 가능성(可能性)을 추구(追究)함이 미래(未來)의 한 과제(課題)가 될 것으로 생각됨. 예(例): 유전공학적(遺傳工學的) 기법(技法)에 의한 성장인자(成長因子)의 개량(改良) 또는 시비효율(施肥效率)의 증대(增大) 작물성장(作物成長)과 미생물활동(微生物活動)과의 상호관계(相互關係) 등(等). 6. 가용(可用) 자원(資源)의 비료화(肥料化)를 위하여 타산업(他産業)의 부산물(副産物) 및 폐기물(廢棄物) (특히 유기질(有機質) 자원(資源))을 효율적(效率的)으로 재활용(再活用)하도록 함.

• Journal of Chest Surgery
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• v.40 no.3 s.272
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• pp.200-208
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• 2007

Background: The effect of patient-prosthesis mismatch (PPM) on the clinical outcome following aortic valve replacement (AVR) remains controversial. This study compared the surgical outcomes of AVR between patients with a patient-prosthesis mismatch and those having undergone an aortic annular enlargement. Material and Method: Six hundred and twenty seven adult patients, who underwent AVR with stented bioprosthetic or mechanical valves, between January 1996 and February 2006, were evaluated. PPM was defined as an indexed effective orifice area (iEOA) ${\leq}0.85cm^2/m^2$, and Severe if the iEOA${\leq}0.65cm^2/m^2$ PPM was present in 103 (16.4%, PPM group) patients, and severe in 11 (1.8%, SPPM group). During the period of the study, 21 patients underwent an AVR with annular enlargement (AE group). Result: The mean iEOA of the AE group was larger than that of the PPM group ($0.95\;vs.\;0.76cm^2/m^2,\;p=0.00$). The AE group had longer CPB, ACC and operation times than the PPM group, and showed a tendency toward higher operative mortality (14.3% vs. 2.9%, p=0.06). The SPPM group had higher AV pressure gradients (peak/mean) than the AE group (72/45 mmHg vs. 38/25 mmHg, p=0.02/0.06) and suffered more AV related events (AV reoperation or severe aortic stenosis)(45.5% vs. 9.5%, p=0.03). LV masses were not regressed in the patients who experienced an AV related event. Conclusion: During AVR in patients with a small aortic annulus, annular enlargement should be carefully applied taking into account the high risk of operative mortality due to annular enlargement and co-morbidities of patients. Aortic annular enlargement; however, should be considered as an alternative method in patients expected to have a severe PPM after an AVR.

• Journal of Biosystems Engineering
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• v.8 no.1
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• pp.47-60
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• 1983

상온통풍(常溫通風)을 이용(利用)한 In-bin drying에 대(對)한 많은 실험결과(實驗結果)에 의(依)하면 우리나라 10월(月)의 기상조건(氣象條件)은 저온건조(低溫乾燥) system에 적합(適合)한 건조능력(乾燥能力)을 가지고 있는 것으로 나타났다. 최근(最近) Computer를 이용(利用)한 Simulation model이 개발(開發)되어 건조현상(乾操現象)에 관(關)한 경제적(經濟的)이고 효율적(效率的)인 분석(分析)이 가능(可能)하게 되었다. 이러한 분석결과(分析結果)에 의(依)하면 초기함수율(初期含水率)이 높은 벼를 Full-bin을 이용(利用)한 상온통풍건조(常溫通風乾操)를 할 경우(境遇) 건조기간(乾燥期間)이 길어지며 bin내(內)의 상층부(上層部) 곡물(穀物)이 변질(變質)되는 문제점(問題點)이 발생(發生)하였다. 또한 벼의 수확작업체계(收穫作業體系)가 관행(慣行) 및 Binder작업체계(作業體系)에서 점차(漸次) Combine작업체계(作業體系)로 전환(轉換)되어감에 따라 포장(圃場)에서의 건조(乾燥)가 어려우며 예취(刈取), 탈곡작업과정(脫穀作業過程)에서의 기계적(機械的)인 곡물(穀物) 손실(損失)을 줄이기 위(爲)하여 함수율(含水率)이 비교적(比較的) 높은 벼를 수확(收穫)하여야 한다. 본(本) 연구(硏究)의 목적(目的)은 상온통풍건조(常溫通風乾燥)에 있어서 건조능력(乾燥能力)을 증가(增加)시키기 위(爲)하여 곡물(穀物)을 일정기간(一定期間) 나누어서 bin에 넣고 건조(乾燥)를 하는 Layer drying의 Simulation model을 개발(開發)하고 이 Model에 수원지방(水原地方)의 7년간(年間) 평균(平均) 기상자료(氣象資料)를 입력(入力)시켜 곡물(穀物)의 초기함수율(初期含水率), 투입량(投入量), 투입기간(投入期間)의 변화(變化)에 따른 Layer drying현상(現象)을 규명(糾明)하는데 있다. Simulation에 사용(使用)된 bin의 크기는 직경(直徑) 2m, 깊이 1.5m이며 송풍(送風)팬의 용량(容量)은 0.5HP이었다. Simulation분석(分析) 결과(結果)를 요약(要約)하면 다음과 같다. (1) Layer drying의 Simulation model을 개발(開發)하였으며 이 Model은 벼의 상온통풍건조(常溫通風乾燥) 실험(實驗)에서 함수량(含水量) 변화(變化)의 이론치(理論値)와 실제실험치(實際實驗値)가 잘 일치(一致)하였다. (2) 곡물투입기간(穀物投入期間) 1일(日)을 Full-bin drying으로 간주(看做)할 때 Layer drying의 건조성능(乾燥性能)은 Full-bin보다 높은 것으로 나타났다. (3) 연속송풍(連續送風)(24시간(時間))을 할 경우(境遇) 곡물투입기간(穀物投入期間)이 증가(增加)함에 따라 건조기간(乾燥期間)의 감소경향(減少傾向)은 단속송풍(斷續送風)인 경우(境遇)보다 적었지만 건조기간(乾燥期間)은 단축(短縮)되었다. 그러나 건조(乾燥)에너지의 소모(消耗)는 단속송풍(斷續送風)일 때보다 크게 나타났다. (4) 단속송풍(斷續送風)(9 : 00AM~6 : 00PM)일 경우(境遇) 곡물투입기간(穀物投入期間)을 증가(增加)시키면 건조기간(乾燥期間)이 크게 줄어 들었다. (5) 곡물(穀物)의 초기함수율(初期含水率)이 21% 이하(以下)일 경우(境遇) 연속(連續) 및 단속송풍(斷續送風)에서 건조기간(乾燥期間)의 차이(差異)가 별로 없었다. (6) 곡물(穀物)의 초기함수율(初期含水率)이 높으면 Full-bin drying에서는 상부층(上部層)에 곡물(穀物)이 변질(變質)될 우려(憂慮)가 있으나 Layer drying에서는 곡물투입량(穀物投入量)을 조절(調節)하면 이것을 방지(防止)할 수 있었다. (7) 건조(乾燥)가 완료(完了)된 후(後) 층별(層別) 최종곡물(最終穀物) 함수율(含水率)은 모든 건조조건(乾燥條件)에서 동일(同一)하였으나 bin의 하부층(下部層)은 과건조(過乾燥) 물상(物象)을 일으켰다.

• Journal of the Korean association of regional geographers
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• v.23 no.1
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• pp.151-167
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• 2017

The main objective of this study was to investigate the climatic impact of $PM_{10}$ concentration on the temperature change pattern in Busan Metropolitan City(BMC), Korea during 2001~2015. Mean $PM_{10}$ concentration of BMC has gradually declined over the past 15 years. While the highest $PM_{10}$ concentration was observed in spring followed by winter, summer, and fall on average, the seasonal variations of $PM_{10}$ concentration differed from place to place within the city. Frequency analysis showed that the most frequently observed $PM_{10}$ concentration ranged from $20{\mu}g/m^3$ to $60{\mu}g/m^3$, which accounted for 64.6% of all daily observations. Overall, the west-high and east-low pattern of $PM_{10}$ concentration was relatively strong during the winter when the effect of yellow-dust events on the air quality was weak. Comparative analyses between $PM_{10}$ concentration and monthly temperature slope derived from generalized temperature curves indicated that the decreasing trend of $PM_{10}$ concentration was associated with increases of annual temperature range, and $PM_{10}$ concentration had a negative relationship with the temperature slope of warming months. Overall, $PM_{10}$ concentration had a weak correlation with the annual mean temperature, but it had a significant, positive correlation with the winter season, which had a dominant influence on the annual mean temperature. In terms of energy budget, it has been known that the change in $PM_{10}$ concentration contributes to the warming or cooling effect by affecting the radiative forcing due to the reflection and absorption of radiant energy. The correlation between $PM_{10}$ concentration and temperature changes in the study area was not seasonally and spatially consistent, and its significance was statistically limited partly due to the number of observations and the lack of potential socioeconomic factors relevant to urban air quality.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.334-341
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• 1984

Yield response of 30 rice varieties widely grown during the past 70 years in Korea was examined at the six transplanting dates from May 10 to June 29 at the 10 day intervals in 1982. Early japonica varieties grown before 1960s showed the highest yield at the June 9 and 19 transplantings and yields decreased with earlier or later transplantings except the earliest transplanting of May 10. However, yield of recent japonica varieties grown after 1960s was similar among the transplanting dates from May 10 to June 19, but yield was lower at the June 29 transplanting. In the indica/japonica varieties grown after mid- 1970s, yield was highest at the May 10, 20, and 30 transplantings and it decreased with further delay of transplantings largely due to the decreased panicle number, percent ripened grains, and 1,000 grain weight. This may indicate that recently developed varieties may be more adaptable toearly transplanting than older varieties. The number of panicles per hill was variable among the transplanting dates in all varieties, but it decreased as transplanting dates delayed after June 9. In japonica varieties the number of spikelets per panicle, percent ripened grains, and 1,000 grain weight increased as transplanting was delayed. However, in indica/japonica varieties the number of spikelets per panicle was similar among the transplanting dates and percent ripened grains and 1,000 grain weight decreased as transplanting date was delayed after June 9. The percent ripened grains of japonica varieties decreased with increase in the number of spikelets per hill but increased as heading date delayed. However, in indica/japonica varieties the percent ripened grains was not affected by both the number of spikelets per hill and heading date except that of June 29 transplanting of which percent ripened grains was extremely low because of delayed heading although the number of spikelets per hill was low. Grain/Straw ratio was not affected by heading date in japonica varieties, but it decreased significantly as heading date was delayed in indica/japonica varieties.

• Journal of Korean Society for Geospatial Information Science
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• v.22 no.3
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• pp.47-56
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• 2014

Recently, the velocity of sea-level rising has increased due to the global warming and the natural disasters have been occurred many times. Therefore, there are various demands for the integration of vertical reference datums for the ocean and land areas in order to develop a coastal area and prevent a natural disaster. Currently, the vertical datum for the ocean area refers to Local Mean Sea Level(LMSL) and the vertical datum for the land area is based on Incheon Mean Sea Level(IMSL). This study uses 31 points of Tidal Gauge Bench Mark (TGBM) in order to compares and analyzes the geometric heights referring LMSL, IMSL, and the nationally determined geoid surface. 11 points of comparable data are biased more than 10 cm when the geometric heights are compared. It seems to be caused by the inflow of river, the relocation of Tidal Gauge Station, and the topographic change by harbor construction. Also, this study analyze the inclination of sea surface which is the difference between IMSL and LMSL, and it shows the inclination of sea surface increases from the western to southern, and eastern seas. In this study, it is shown that TGBM can be used to integrate vertical datums for the ocean and land areas. In order to integrate the vertical datums, there need more surveying data connecting the ocean to the land area, also cooperation between Korea Hydrographic and Oceanographic Administration and National Geographic Information Institute. It is expected that the integrated vertical datum can be applied to the development of coastal area and the preventative of natural disaster.