• Journal of the Korean Chemical Society
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• v.57 no.1
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• pp.81-87
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• 2013

Staphylococcus aureus is the most important pathogen in nosocomial infections, including bloodstream infections. Prompt identification of S. aureus from blood cultures and detection of methicillin resistance are essential in cases of suspected sepsis. We have studied a new method for the sequence-specific visual detection of minute amounts of nucleic acids using intercalating reaction by addition of SYBR Green to amplicons of LAMP, and it's a unique gene amplification method in which DNA can be isothermally amplified using only one enzyme. Staphylococcus-LAMP, which targets the spa gene, encoding S.aureus-specific protein A, and the mecA gene, encoding penicillin-binding protein-2' for methicillin resistance, detected MRSA and MRSE. In this study, by using LAMP assay, I detected for Staphylococcus aureus and Staphylococcus epidermidis concentration in the clinical sample. The detection of Staphylococcus aureus and Staphylococcus epidermidis was tested by using serial 10-fold dilutions standard solution. I have accurate detected the limit of detection, sensitity, specificity and reproducibility of the assay. The Bio-chip based LAMP assay allowed easy, rapid, accurate and sensitive detection of infection with Staphylococcus and especially applicable in a resource-limited situation.

• Journal of Service Research and Studies
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• v.12 no.4
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• pp.82-93
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• 2022

The food service industry is a major driver of global sustainable food consumption. By understanding food consumption behavior, restaurant managers can forecast demands and reduce pre-consumer food waste. This study investigates the relationship between influencing factors and the number of customers at restaurants and cafés. These factors are weather-related factors, including rain and temperature, and school-related factors, including exams and the day of the week. Based on these four factors, 24 possible combinations were created. Three representtive days were chosen for each weekday combination. Besides, one representative day was chosen for each weekend combination. In total, 48 days were sampled throughout the year. Customer data were collected from six restaurants and cafes on a Korean university campus. Conjoint analysis was used to determine the relative importance of each variable to customer numbers. Following that, utility scores were standardized and mapped to determine the best condition when the number of customers was at its peak. In addition, each store's sales were compared using Pearson's Correlation Coefficient. The findings support that temperature and rain influences are correlated with the number of customers. Furthermore, we discovered that temperature was far more significant than rain in determining the number of customers. The paper discusses the implications of weather to forecast food and beverage demand and predict meal choices.

• Tunnel and Underground Space
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• v.33 no.6
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• pp.445-471
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• 2023

Long-term safety over millennia is the top priority consideration in the construction of disposal sites. However, ensuring the mechanical stability of deep geological repositories for spent fuel, a.k.a. radwaste, disposal during construction and operation is also crucial for safe operation of the repository. Imposing restrictions or limitations on tunnel support and lining materials such as shotcrete, concrete, grouting, which might compromise the sealing performance of backfill and buffer materials which are essential elements for the long-term safety of disposal sites, presents a highly challenging task for rock engineers and tunnelling experts. In this study, as part of an extensive exploration to aid in the proper selection of disposal sites, the anticipation of constructing a deep geological repository at a depth of 500 meters in an unknown state has been carried out. Through a review of 2D and 3D numerical analyses, the study aimed to explore the range of properties that ensure stability. Preliminary findings identified the potential range of rock properties that secure the stability of central and disposal tunnels, while the stability of the vertical tunnel network was confirmed through 3D analysis, outlining fundamental rock conditions necessary for the construction of disposal sites.

• Journal of the Korean Arthroscopy Society
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• v.13 no.1
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• pp.46-52
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• 2009

Purpose: To evaluate the usefulness of minimally invasive arthroscopy-assisted plate removal of a laterally inserted periarticular distal femur plate used for the treatment of AO type-C distal femur fractures. Materials and Methods: From October 2002 to November 2005, we evaluated 17 patients whose plates were removed through minimally invasive arthroscopy-assisted plate-removal technique and 15 patients who got their plates removed through conventional method without using arthroscopy, 32 patients in total. All these patients included in this study initially underwent open reduction and internal fixation of the distal femoral fractures with a lateral plate, and complained of continued pain over the lateral femoral condyle after the fracture fixation. The average age was 42.6 (ranges: 20~66) and initial fracture types included 16 cases of C1, 11 cases of C2, and 5 cases of C3 following AO/ASIF classification guidelines. Measured outcomes included: associated intra-articular pathologies, time needed to return to activities of daily living, patients' overall satisfaction, complications following the removal of hardware, and pain before and 6 months after the operation. Results: The distal-most end of the plate was placed in the knee joint in all cases and damage of the lateral articular capsule was found in 23 cases. Continuous wound discharge after surgery was found in one case who underwent arthroscopy-assisted plate removal, and it was treated by irrigation and re-suture. Average time needed to return to activities of daily living was 7 days in arthroscopy assisted group and 7.6 days in conventionally removed group. Fourteen patients (82.4%) who underwent arthroscopyassisted plate-removal reported above 'fair' satisfaction and the Visual analog scale pain score decreased from 4.9 to 1.9, six months after the plate removal. Thirteen patients(86.7%) who underwent conventional plate removal reported above 'fair' satisfaction and the Visual analog scale pain score decreased from 5.2 to 2.5, six months after the operation. Conclusion: Through minimally invasive arthroscopic-assisted plate removal, intrarticular pathology of the knee joint was able to be simultaneously identified and treated at the time of hardware removal. Damage of lateral capsule of the knee joint caused by the inserted plate for the treatment of type C distal femoral fracture was very frequently found and following the plate removal, patients experienced an improvement in pain score. We therefore recommend routine lateral distal femoral plate removal if the bony union is attained in such cases as type C distal femoral fractures whose distal most end of the plates are located in the joint.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.146-152
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• 2007

The present study was carried out to investigate the potential acute toxicity of ethanol extracts of the aleurone layer of Oryza sativa cvs. Obongbyeo, Ilpumbyeo, and Aranghyangchalbyeo by a single oral dose in ICR mice. The test article was orally administered once by gavage to male and female mice at dose levels of 0, 2.5, 5.0, and 10.0 g/kg body weight (n=10 for male and female mice for each dose). We examined numbers of deaths, general signs, weight measurement and biochemical analysis for sexes and doses of mice control and experimental groups. All mice were alive during the experimental period so can not yield death rate and $LD_{50}$. Any significant clinical symptom was not observed in all treated groups. No significant body weight changes in treatment groups in comparison with those of control groups was observed at any dose levels in experimental groups. Plasma glucose levels were valued both control and treated groups and there were no significant differences between groups. The activities of ALT and AST were not increase in all sample treated groups when compared with the control groups. The results suggest that the toxicity of Oryza sativa cvs. Obongbyeo, Ilpumbyeo, and Aranghyangchalbyeo are low and its $LD_{50}$ is over 10.0 g/kg body weight in both male and female mice.

• Journal of dental hygiene science
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• v.14 no.4
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• pp.571-579
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• 2014

This study was conducted to provide basic data for improvement of the oral health management ability and the factors affecting oral health recognition and practice of 6th graders. The research subjects total 259 numbers of 6th grade students in two schools located in Changwon, it was investigated and analyzed with structured questionnaires. The collected data is analyzed by using IBM SPSS Statistics 19.0. The average oral health recognition score investigated were $3.19{\pm}0.41$, and $2.95{\pm}0.43$ for oral health practices. The oral health recognition was statistically significant differences by father's education (p<0.01), perceived oral health status (p<0.01), perceived grade (p<0.01), intention to attend oral health education (p<0.001). The oral health practices was statistically significant differences by father's education (p<0.01), perceived oral health status (p<0.001), perceived grade (p<0.05), parent's interest in child's toothbrushing (p<0.01), experience of oral health education (p<0.001), intention to attend oral health education (p<0.05). The factors that may improve the oral health recognition are in order of oral health practices, intention to attend oral health education, and parent's attention to child's tooth brushing. The factors that may improve the oral health practice was found to be in order of oral health recognition, parent's attention to child's tooth brushing, and perceived oral health status. As a result, in order to improve oral health management ability of 6th grade students, the surrounding environment, the attitude on oral care, the interest of oral, and the current disease status of oral health are required to be identified, then the oral health education program should be provided.

• Journal of Food Hygiene and Safety
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• v.13 no.4
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• pp.319-331
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• 1998

The purposes of this study were to develop Hazard Analysis Critical Control Point plan applicable to cook/chilled Pork Bulkogi (broiled sliced pork with sauces) in school foodservice operations and to establish reasonable shelf-life limits by assessing food quality during chilled storage period of 5 days. During the product flow, time-temperature profile was recorded and microbiological analyses including mesophilic and psychrotrophic total plate counts, coliform, and fecal coliform and qualitative analyses of Salmonella and Listeria monocytogenes were done. Chemical analyses (pH, acid value, total volatile basic nitrogen), sensory evaluation, and quantitative analysis of thiamin were conducted for 5 days of chilled storage. The number of mesophiles in raw pork ($4.26{\pm}0.11\;Log\;CFU/g$), seasoning mixture ($5.97{\pm}O.04\;Log\;CFU/g$) and marinated pork ($5.56{\pm}0.21\;Log\;CFU/g$) were below the microbial standards for "requires further cooking" food items. Listeria monocytogenes was detected in seasoning mixture. After heating, the number of mesophiles ($5.17{\pm}0.04\;Log\;CFU/g$) were slightly reduced but it did not meet the microbial guidelines of $5\;Log\;CFU/g$ for "ready-to-eat" foods. No other microbes including pathogens were detected. By reheating the menu item after chilled storage, the number of mesophiles were reduced in every phase of 1st day ($4.62{\pm}0.22\;Log\;CFU/g$), 3rd day ($4.55{\pm}0.20\;Log\;CFU/g$) and 5th day ($4.25{\pm}0.16\;Log\;CFU/g$) of chilled storage, and the number of microbes was below the standard limits for "ready-to-eat" foods. At the fifth day of chilled storage, pH (p<0.05), acid value (p<0.01) and TVBN (p<0.05) showed significant increases. Sensory evaluation results did not show any significant change for 5 days of chilled storage. Thiamin content showed a decrease for 5 days of chilled storage. Consequently, the ideal shelflife recommended for Pork Bulkogi was within 3 days of chilled storage. CCPs for Pork Bulkogi were purchasing and receiving of raw meat and some seasoning ingredients, heating, chilling, chilled storage, reheating, and distribution.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.39 no.1
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• pp.37-42
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• 2021

Munseom (Island) is known as the largest native Elaeocarpus sylvestris var. ellipticus in Korea. Recently, disease damage from the E. sylvestris var. ellipticus community has been reported in Munseom. This study was conducted to understand the damage situation and growth characteristics of the E. sylvestris var. ellipticus population in Munseom. The purpose of this study was to evaluate the spontaneous regeneration potential of the community by grasping the characteristics of the population of surviving E. sylvestris var. ellipticus individuals, and to discuss ways to restore native habitats. A total population survey was conducted to determine the distribution status of E. sylvestris var. ellipticus individuals. The field survey carefully recorded the height of tree, DBH, DRH, and growing status, along with GPS location information of the individual. The growth status of E. sylvestris var. ellipticus individuals distributed in Munseom and the characteristics of tree height, DBH, DRH of each individual were analyzed. The total number of E. sylvestris var. ellipticus populations identified in Munseom was 293. The dense E. sylvestris var. ellipticus community reported in 2005 has been identified as being greatly damaged or damaged. In particular, the damage was more serious in mature trees with a height of 6m or more forming the canopy layer in the forest, and it was confirmed that 80.6% of the mature trees died. In the growth characteristics of the old-growth tree, which is an indicator of the health of the forest based on DRH, 75.4% died or the growth condition was found to be poor. In order to restore the native habitat of E. sylvestris var. ellipticus in Munseom, conservation efforts such as pest control, preservation of mature trees, control of the territory of young trees, and reintroduction after ex-situ transplantation etc. are considered necessary.

• Geophysics and Geophysical Exploration
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• v.1 no.2
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• pp.101-109
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• 1998

Using airborne magnetic data, magnetic characteristics were studied at the Samryangjin caldera area developed in the volcanics of the Yuchon sub-basin, the south eastern part of the Gyeongsang basin. Residual magnetics, reduction to the pole, horizontal derivative, and vertical derivative maps are prepared. Using these maps, the magnetic lithofaces are zoned and the geological structures such as caldera and faults were qualitatively interpreted. In addition, the two quantitative interpretations were performed. Firstly, the forward modelling were done to the 14.5 line km crossing the caldera area to the northeast-southwest direction. Applying the 3-D Euler deconvolution method to the whole study area, the depth extent and the characteristics of the magnetic anomalous bodies were studied. According to the results, the magnetic lithofaces of the area are zoned by 4 units. In general, these are well matched with the geological distributions. But the biotite granites intruded in the northern boundary of the Samryangjin caldera show the high magnetic intensity, while the biotite granites of the other areas show the low magnetic intensity and the different magnetic lithofaces. Thus, we interpreted that the biotite granites are closely related with the volcanic activity of the Samryngjin caldera, and are intruded in the fracture zones developed along the caldera rim. The Samryangjin caldera and fault structures of the area can be easily recognized by the distinct magnetic structures from the various magnetic anomaly maps. Also the topographic characteristics well reflect these structures. The results of the forward modelling show that the magnetic basement depth of the Gyeongsang sedimentary basin is on the average about 6 km and in maximum 10 km. And the depth becomes shallower toward the caldera boundary due to the shallow intrusion of the volcanics. The results of the 3-D Euler method also show the caldera and fault structures. And the relatively shallow magnetic anomalous bodies which are related with the volcanics are generally developed to the east-west and northeast directions, while the deep magnetic anomalous bodies to the northwest direction.

• Journal of the Korea Concrete Institute
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• v.14 no.1
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• pp.126-135
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• 2002

Plate bonding technique has been widely used in strengthening of existing concrete structures, although it has often a serious problem of premature falure such as interface separation and rip-off. However, this premature failure problem has not been well explored yet especially in view of local failure mechanism around the interface of plate ends. The purpose of the present study is, therefore, to identify the local failure of strengthened plates and to derive a separation criterion at the interface of plates. To this end, a comprehensive experimental program has been set up. The double lap pull-out tests considering pure shear force and half beam tests considering combined flexure-shear force were performed. The main experimental parameters include plate thickness, adhesive thickness, and plate end arrangement. The strains along the longitudinal direction of steel plates have been measured and the shear stress were calculated from those measures strains. The effects of plate thickness, bonded length, and plate end treatment have been also clarified from the present test results. Nonlinear finite element analysis has been performed and compared with test results. The Interface properties are also modeled to present the separation failure behavior of strengthened members. The cracking patterns as well as maximum failure loads agree well with test data. The relation between maximum shear and normal stresses at the interface has been derived to propose a separation failure criterion of strengthened members. The present study allows more realistic analysis and design of externally strengthened flexural member with steel plates.