• The Journal of the Korea Contents Association
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• v.22 no.3
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• pp.567-575
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• 2022

This study was carried out to elucidate bioacive effects of three domestic cherry tomato cultivars. Total polyphenol and flavonoid of Summerking, Qutiquti, and Minichal cultivar were 12.56±1.88, 12.50±1.92, 11.65±1.85 mg/g and 4.58±1.03, 4.19±0.40, 4.30±0.49 mg/g(dry weight) respectively. Domestic cherry tomatoes showed antioxidative activity(DPPH and ABTS radical scavenging activities). All of the cherry tomatoes had no cytotoxicity for normal liver cell, but showed strong inhibitory effect against cervical cancer cell(HeLa) growth. These results revealed that domestic cherry tomatoes can be used as a bioactive food material.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.05a
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• pp.14-14
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• 2002

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.433-440
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• 2013

Nine kinds of flowers were selected by its antioxidative activities evaluated. DPPH(1,1-diphenyl-2-picryl hydrazil), reducing power, total phenol contents, total flavonoid contents, and antimicrobial activity inhibitory effects of nine natural flower varieties were examined using ethanol extract (80%, v/v). DPPH radical scavenging of Agastache rugosa (fisch.&Mey.) kuntze ($IC_{50}=74.6{\mu}g/mL$) and solidago virga-aurea var. asiatica ($IC_{50}=99.6{\mu}g/mL$) showed higher antioxidant activity compared with those of the other varieties. Reducing power of Agastache rugosa (fisch.&Mey.) kuntze ($OD_{700}=1.0$) had higher antioxidant activity. Agastache rugosa (fisch.&Mey.) kuntze showed the highest content of total phenol (134.6 mg GAE/g). However, total flavonoid (554.6 mg QE/g) exhibited the lowest. These results suggest that nine kinds of flower with 80% ethanol extracts have significant antioxidant activity.

• Korean Journal of Food Science and Technology
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• v.53 no.3
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• pp.252-259
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• 2021

Barley contains health-beneficial compounds, such as phenolic acids, flavonoids, lignans, and tocopherols. The antioxidant activities of pearled and wholegrain hull-less, premature, and black barley harvested in Jeju were investigated by measuring total phenolic content (TPC), total flavonoid content (TFC), phenolic compounds, tocopherols (T), and tocotrienols (T3), along with their in vitro antioxidant activities. Consequently, TPC and TFC in wholegrain barley groups were higher than those in pearled barley. Gallic acid (1.55-2.98 ㎍/g) and protocatechuic acid (0.67-2.84 ㎍/g) were the predominant phenolic compounds in barley. Total T and T3 concentrations of wholegrain barley groups were significantly higher than those of pearled barley (p<0.05). Except for the metal chelating effect and reducing power, the in vitro antioxidant activities of wholegrain barley were significantly higher than those of pearled barley. These results indicate that wholegrain Jeju barley can be used as a natural antioxidant source in the food industry.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.65-65
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• 2023

제주도에서 재배되어 온 재래귤 5종(당유자, 유자, 청견, 하귤, 팔삭) 부위별 에탄올 추출물의 총 폴리페놀 함량 측정, 총 플라보노이드 함량 측정, DPPH radical 소거 활성 실험을 실시하였다. 총 폴리페놀 함량의 경우, 5종 재래귤(당유자, 유자, 청견, 하귤, 팔삭) 부위별 에탄올 추출물 가운데 당유자의 외피(244.84mg/g)와 중피(200.70mg/g) 처리구에서 폴리페놀 함량이 가장 높았다. 총 플라보노이드 함량의 경우, 총 폴리페놀의 함량 측정에서와 마찬가지로 5종 재래귤(당유자, 유자, 청견, 하귤, 팔삭) 부위별 에탄올 추출물 가운데 당유자의 외피에서 102.12mg/g으로 가장 높았으며, 청견과 하귤, 팔삭의 플라보노이드 함량은 폴리페놀 처리구와 같이 당유자 처리구보다 적게 나타났다. DPPH radical 소거 활성의 경우, 총 폴리페놀 함량과 총 플라보노이드 함량 측정의 결과에 같은 경향으로 5종 재래귤(당유자, 유자, 청견, 하귤, 팔삭) 부위별 에탄올 추출물 가운데 당유자의 외피(79.39%)와 중피(70.73%)에서 가장 양호한 항산화 활성을 나타내었다. 이러한 결과들로부터 당유자의 두꺼운 과피 구조인 외피와 중피에 에센셜 성분인 limonene, myrcene, β-ocimenes 등과 같은 향기성분들의 영향으로 생각되어 앞으로 이에 대한 연구가 더 필요하리라 생각된다.

• Tuberculosis and Respiratory Diseases
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• v.53 no.5
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• pp.530-541
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• 2002

Background : Pleural fluid adenosine deaminase (ADA) activity can be helpful in a differntial diagnosis of an exudative pleural effusion because it is increased in a tuberculous pleural effusion. The ADA activity is determined mainly by the lymphocyte function. Age-associated immune decline is characterized by a decrease in T-lymphocyte function. For that reason, the pleural fluid ADA level would be lower in older patients with exudative pleural effusion. This study focused on the influence of age on the pleural fluid ADA activity in patients with exudative pleural effusion. Methods : A total of 81 patients with exudative pleural effusion were enrolled in this study. In all patients, the pleural fluid ADA activity was measured using an automated kinetic method. Results : The mean age of the patients was $52.7{\pm}21.2$ years. In all patients with exudative pleural effusion, the pleural fluid ADA activity revealed a significant difference between young patients (under 65 years of age) and old patients (p<0.05), and showed a negative correlation with age (r=-0.325, p<0.05). In the 60 patients with a tuberculous pleural effusion, the pleural fluid ADA activity revealed a significant difference between the young and older patients : $103.5{\pm}36.9$ IU/L in young patients Vs. $72.2{\pm}31.6$ IU/L in old patients (p<0.05), and showed a negative correlation with age (r=-0.384, p<0.05). In the 21 patients with non-tuberculous exudative pleural effusion, the pleural fluid ADA activity of the young patients and old patients was similar : $23.7{\pm}15.3$ IU/L in young patients Vs. $16.1{\pm}10.2$ IU/L in old patients (p>0.05), and did not show any correlation with age (r=-0.263, p>0.05). The diagnostic cutoff value of pleural fluid ADA activity for tuberculous pleural effusion was lower in the older patients (25.9 IU/L) than in the younger patients (49.1 IU/L) or all patients (38.4 IU/L) with exudative pleural effusion. Conclusion : Tuberculous pleural effusion is an important possibility to consider in older patients with a clinical suspicion of a tuberculous pleural effusion, although no marked increase in the pleural fluid ADA activity is usually detected. For a diagnosis of a tuberculous pleural effusion in old patients, the cutoff for the pleural fluid ADA activity should be set lower.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.3
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• pp.325-334
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• 2013

The objective of this study was to compare the content and metabolic activities between fresh pine needle juice (PNJ) and fermented pine needle juice (FPNJ). A variety of factors were measured, including total phenolic content (TPC), antioxidant activity [DPPH radical scavenging activity (RSA), total radical-trapping antioxidant potential (TRAP), oxygen radical absorbance capacity (ORAC), cellular antioxidant capacity (CAC)], anti-genotoxic activity, ${\alpha}$-glucosidase inhibitory activity, and angiotensin converting enzyme (ACE) inhibitory activity. The TPC was $17.3{\pm}0.2$ and $4.6{\pm}0.0$ mg GAE/g in PNJ and FPNJ, respectively. The DPPH RSA, TRAP, and ORAC values increased in a dose-dependent manner for both PNJ and FPNJ, with significantly higher activities in PNJ than FPNJ. The CAC against AAPH-induced oxidative stress in HepG2 cells was protected by both PNJ and FPNJ. Pretreatment with PNJ and FPNJ in human leukocytes produced significant reductions in $H_2O_2$-induced DNA damage at a concentration of $50{\mu}g/mL$. ${\alpha}$-Glucosidase inhibitory activity was significantly higher in FPNJ than PNJ. The ACE inhibitory activity was about 87.1% and 60.0% in 1:1 diluted PNJ and FPNJ, respectively. This study suggests that the fermentation of PNJ could enhance the regulation of blood glucose metabolism and both PNJ and FPNJ might be a new potential source of natural antioxidant, anti-diabetic, and anti-hypertensive agents applicable to food.

• Current Research on Agriculture and Life Sciences
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• v.31 no.3
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• pp.209-216
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• 2013

This study compared the antioxidant activity and functional constituents of the peel, flesh,and placenta of oriental melons according to the harvest time. The sample oriental melons were harvested during the major harvest time (from June to August) and divided into peel, flesh, and placenta. To examine the antioxidant activity and functional constituents, the sample melons were extracted using EtOH. As a result, the functional constituents and antioxidant activity of the oriental melons were found to change according to the harvest time. The total phenol and total flavonoid contents were highest in the samples harvested in June, and lowest in the samples harvested in July. Meanwhile, the antioxidant activities were estimated using an ABTS and FRAP assay, where the samples harvested in June also showed the strongest the antioxidant potential, while the samples harvested in July showed significantly lower antioxidant activities. This means that the climate influences the synthesis of secondary metabolites. The above data also suggests that oriental melons harvested in June contain more functional phytochemicals, making them more beneficial for human health.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.67-67
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• 2023

수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임) 부위별 에탄올 추출물의 항산화 활성에 관하여 총 폴리페놀 함량 측정, 총 플라보노이드 함량 측정, DPPH radical 소거 활성 실험을 실시하였다. 수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임)의 각 부위별 생리활성 실험에서 총 폴리페놀 함량(mg/g)은 청자몽 중피(252.14mg/g)와 청자몽 외피(224.60mg/g), 청자몽 과피(213.13mg/g), 자몽 중피(197.13mg/g) 순으로 모두 150mg/g 이상의 양호한 폴리페놀 함량을 나타내었다. 수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임)의 각 부위별 총 플라보노이드 함량(mg/g)은 자몽 중피(147.24mg/g)와 청자몽 중피(147.08mg/g), 청자몽 과피(127.74mg/g), 청자몽 외피(122.5mg/g) 순으로 모두 120mg/g 이상의 매우 양호한 플라보노이드 함량을 나타내었다. 수입귤 6종(레몬, 자몽, 청자몽, 오렌지, 만다린, 라임)의 각 부위별 항산화 활성(EDA, %)은 레몬 과피(83.21%)를 시작으로 라임 전체(80.23%), 레몬 과육(77.02%), 레몬 전체(69.85%), 만다린 과피(68.86%), 라임 과육(64.61%) 순으로 EDA 50% 이상 값을 나타내어 양호한 항산화 활성을 보였다.

• Journal of Life Science
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• v.10 no.3
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• pp.254-261
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• 2000

In order to observe the bioactivity of ovariectomized rats, ovariectomized group (Ovx), nonovariectomized group (Sham), ovariectomized Vitamin C-treat group (Ovx+Vit C), ovariectomized Vitamin E-treat group (Ovx+Vit E) and ovariectomized Vitamin C+Vitamin E-treat group(Ovx+Vit C+E) were made. Lipidperoxides of liver and kidney, serum total cholesterol and HDL-cholesterol were investigate as follows. Lipidperoxides of liver and kidney in Ovx group were 1.78 times and 1.61 times increased compared to Sham group respectively. But, they were significantly decreased in Ovx+Vit C group, Ovx+Vit E group, Ovx+Vit C+E group compared to Ovx group. Serum total cholesterol in Ovx group was increased 2.57 times compared to Sham group. Injections of each substance such as ascorbate, tocopherol, mixture (C+E) make data of Cholesterol become low. When especially Vit C is injected, the data of cholesterol lowed by about 94%. Serum HDL-cholesterol in Ovx group decreased 36.7% compared to Sham group. And as the result of the measurement of SOD, Catalase, and GPx which are antioxidant enzyme, SOD and Catalase activities in Ovx group much higher than in Sham group. Based on the results, it is supposed that more produced free radicals increased antioxidant enzyme. And it is also thought that vitamin can inhibit aging by reducing antioxidant enzyme.