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Comparative Study of Native Flowers for Anti-oxidative Effects in Korea

국내 자생 꽃 품종에 따른 항산화활성 비교

  • Sa, Yeo Jin (Department of Applied Plant Sciences, Kangwon National University) ;
  • Park, Jong Hyuk (Oriental Bio-herb Research Institute, Kangwon National University) ;
  • Kim, Dong Hyun (Research and Development Center, Amorepacific Corporation) ;
  • Yeom, Myeong Hun (Research and Development Center, Amorepacific Corporation) ;
  • Cho, Jun Cheol (Research and Development Center, Amorepacific Corporation) ;
  • Kwon, Yong Soo (Department of Pharmacy, Kangwon National University) ;
  • Kim, Myong Jo (Department of Applied Plant Sciences, Kangwon National University)
  • 사여진 (강원대학교 식물자원응용공학부) ;
  • 박종혁 (강원대학교 한방바이오연구소) ;
  • 김동현 ((주)아모레퍼시픽 기술연구원) ;
  • 염명훈 ((주)아모레퍼시픽 기술연구원) ;
  • 조준철 ((주)아모레퍼시픽 기술연구원) ;
  • 권용수 (강원대학교 약학대학) ;
  • 김명조 (강원대학교 식물자원응용공학부)
  • Received : 2013.04.01
  • Accepted : 2013.08.08
  • Published : 2013.08.31

Abstract

Nine kinds of flowers were selected by its antioxidative activities evaluated. DPPH(1,1-diphenyl-2-picryl hydrazil), reducing power, total phenol contents, total flavonoid contents, and antimicrobial activity inhibitory effects of nine natural flower varieties were examined using ethanol extract (80%, v/v). DPPH radical scavenging of Agastache rugosa (fisch.&Mey.) kuntze ($IC_{50}=74.6{\mu}g/mL$) and solidago virga-aurea var. asiatica ($IC_{50}=99.6{\mu}g/mL$) showed higher antioxidant activity compared with those of the other varieties. Reducing power of Agastache rugosa (fisch.&Mey.) kuntze ($OD_{700}=1.0$) had higher antioxidant activity. Agastache rugosa (fisch.&Mey.) kuntze showed the highest content of total phenol (134.6 mg GAE/g). However, total flavonoid (554.6 mg QE/g) exhibited the lowest. These results suggest that nine kinds of flower with 80% ethanol extracts have significant antioxidant activity.

국내에 자생하는 꽃 중 9종을 80% ethanol 추출 및 농축하여 전자공여능, 환원력, 총 페놀 함량, 총 플라보노이드 함량을 측정한 결과, 전자공여능에서 배초향이 ($SC_{50}=74.6{\mu}g/mL$) 대조군인 BHT($SC_{50}=238.8{\mu}g/mL$)보다 월등히 높은 활성을 보였다. 환원력 측정 결과 추출물의 농도가 높아짐에 따라 활성이 증가하는 경향을 보였으며, 9개 품종의 꽃 중에서 배초향이 1.0의 OD값으로 가장 높은 활성을 나타내었다. 또한 추출물의 총 페놀 함량은 배초향이 134.6mg GAE/mL로 높은 함량을 나타내었으며, 벌개미취가 40.1 mg GAE/mL로 가장 낮은 함량을 보였다. 총 플라보노이드 함량은 고본이 87.0 mg QE/mL로 높은 함량을 보였다. 이러한 결과로 총 페놀 함량과 항산화능과의 상관관계가 있는 것을 확인할 수 있었다. 꽃 중에서 가장 항산화 활성이 높은 배초향에 대해 용매 분획을 실시하여 DPPH radical소거활성 실험을 수행한 결과, ethyl acetate층이 $SC_{50}=43.2{\mu}g/mL$로 높은 활성을 나타내었다. 또한 총 페놀 및 플라보노이드 함량은 각각 235.5 mg GAE/mL, 96.0 mg QE/mL로 다른 분획물보다 높은 함량을 나타내었다. 본 연구는 결과를 통하여 꽃의 활용성을 증가시킨다고 판단한다. 꽃 추출물의 생리활성 검정은 새로운 기능성 소재로서의 가치를 평가하는데 있어 기초자료 제공에 많은 기여를 할 수 있을 것이며, 꽃을 이용한 천연항산화제의 개발과 꽃에 함유되어 있는 유효성분 연구가 활발하게 이루어질 것이다.

Keywords

References

  1. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181:1199-1200. https://doi.org/10.1038/1811199a0
  2. Hyun, M.R., Y.S. Lee and Y.H. Park. 2011. Antioxidative activity and flavonoid content of Chrysanthemum zawadskii flowers. Kor. J. Hort. Sci. Technol. 29(1):68-73 (in Korean).
  3. Joung, Y.M., S.J. Park, K.Y. Lee, J.Y. Lee, J.K. Suh, S.Y. Hwang, K.E. Park and M.H. Kang. 2007. Antioxidative and antimicrobial activities of Lilium species extracts prepared from different aerial parts. Korean J. Food Sci. Technol. 39(4):452-457 (in Korean).
  4. Jung, Y.T., I.S. Lee, K. Whang and M.H. Yu. 2012. Antioxidant effects of Picrasma quassioides and Chamaecyparis obtuse (S. et Z.) ENDL extracts. J. Life Sci. 22(3):354-359 (in Korean). https://doi.org/10.5352/JLS.2012.22.3.354
  5. Kim, M.J., J.S. Choi, E.J. Song, S.Y. Lee, K.B.W.R. Kim, S.J. Lee, S.J. Kim, S.Y. Yoon, Y.J. Jeon and D.H. Ahn. 2009. Effects of heat and pH treatments on antioxidant properties of Ishige okamurai extract. Korean J. Food Sci. Technol. 41(1):50-56 (in Korean).
  6. Kim, D.H., B.J. An, S.G. Kim, T.S. Park, G.H. Park and J.H. Son. 2011. Anti-inflammatory effect of Ligularia fischeri, Solidago virga-aurea and Aruncus dioicus complex extracts in raw 264.7 cells. J. Life Sci. 21(5):678-683 (in Korean). https://doi.org/10.5352/JLS.2011.21.5.678
  7. Kim, E.M. 2011. Antioxidant and anticancer effects of extracts and components from Artemisia princeps Pampanini and Cirsium setidens Nakai. J. East Asian Soc. Dietary Life 21(6):871-876 (in Korean).
  8. Kim, H.J., C.O. Hong, M.H. Nam, Y.M. Ha and K.W. Lee. 2012a. Antioxidant and physiological activities of Capsicum annum ethanol extracts. J. Korean Soc. Food Sci. Nutr. 41(6):727-732 (in Korean). https://doi.org/10.3746/jkfn.2012.41.6.727
  9. Kim, Y.H., J.Y. Han, J.H. Sung, M.S. Sung, Y.M. Choi, H.S. Jeong and J.S. Lee. 2012b. Anti-inflammatory activity of Chrysanthemum zawadskii var. latilobum leaf extract through haem oxygenase-1 induction. J. Functional Foods 4:474-479. https://doi.org/10.1016/j.jff.2012.02.007
  10. Lee, J.H. and S.R. Lee. 1994. Some physiological activity of phenolic substances in plant foods. Korean J. Food Sci. Technol. 26(3):317-323 (in Korean).
  11. Lee, H.W., J.H. Choi, S.Y. Park, B.K. Choo, J.M. Chun, A.Y. Lee and H.K. Kim. 2008. Constituents comparison of components in native and cultivated species of Angelica tenuissima Nakai. Korean J. Medicinal Crop Sci. 16(3): 168-172 (in Korean).
  12. Lee, S.M., Y.H. You, K.M. Kim, J.J. Park, C.S. Jeong, D.Y. Jhon and W.J. Jun. 2012. Antioxidant activities of native gwangyang Rubus coreanus Miq. J. Korean Soc. Food Sci. Nutr. 41(3):327-332 (in Korean). https://doi.org/10.3746/jkfn.2012.41.3.327
  13. Moreno, M.I.N, M.I. Isla, A.R. Sampietro and M.A. Vattuone. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnophamacol. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  14. Moon, Y.G., J.S. Hong and M.H. Song. 2012. DPPH radical scavenging activity and composition of essential oil from the herbs of jeju Agastache rugosa. J. Life Sci. 22(2):156-160 (in Korean). https://doi.org/10.5352/JLS.2012.22.2.156
  15. Oyaizu, M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  16. Park, Y.J., H.J. Kim and B.G. Heo. 2007. An in vitro study on total phenol content, electron donor capacity and their cytotoxicity effects of extracts of four different edible flowers. Flower Res. J. 15(1):41-45 (in Korean).
  17. Pasko, P., H. Barton, P. Zagrodzki, S. Gorinstein, M. Folta, Z. Zachwieja. 2009. Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth. Food Chem. 115: 994-998. https://doi.org/10.1016/j.foodchem.2009.01.037
  18. Park, Y.H., S.H. Lim, H.Y. Kim, M.H. Park, K.J. Lee, K.H. Kim, Y.G. Kim and Y.S. Ahn. 2011. Biological activities of extracts from flowers of Angelica gigas Nakai. J. Korean soc. Food Sci. Nutr. 40(8):1079-1085 (in Korean). https://doi.org/10.3746/jkfn.2011.40.8.1079
  19. Shim, S.Y. and D.S. Byun. 2011. Inhibitory effects of Chrysanthemum zawadski ethanolic extract on $Fc{\varepsilon}RI$ $\alpha$ chain expression. Korean J. Food Sci. Technol. 43(2): 220-223 (in Korean). https://doi.org/10.9721/KJFST.2011.43.2.220
  20. Taga, M.S., E.E. Miller and D.E. Pratt. 1984. Chia seeds as a source of natural lipid antioxidants. J. Am. oil Chem. 61: 928-993. https://doi.org/10.1007/BF02542169
  21. Vrcek, I.V., M. Bojic, I. Zuntar, G. Mendas and M. Medic- Saric. 2011. Phenol content, antioxidant activity and metal composition of croatian wines deriving from organically and conventionally grown grapes. Food Chem. 124:354-361. https://doi.org/10.1016/j.foodchem.2010.05.118
  22. Woo, J.H., S.L. Shin, Y.D. Chang and C.H. Lee. 2009a. Comparison of antioxidant effects by different extraction methods in flowers of Aster scaber, Aster maackii, Coreopsis lanceolata and Coreopsis tinctoria. Korean J. Plant Res. 22(5):381-388 (in Korean).
  23. Woo, J.H., S.L. Shin, Y.D. Chang and C.H. Lee. 2009b. Antioxidant activities of Chrysanthemum frutescens, Coreopsis lanceolata, Matricaria recutica and Hieractium pilosella flower extracts by extraction solvent. Flower Res. J. 17(3):158-164 (in Korean).
  24. Woo, J.H., S.L. Shin and C.H. Lee. 2010. Antioxidant effect of 80% ethanol extracts obtained from three Dendranthema species. Korean J. Plant Res. 23(1):47-53 (in Korean).

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