• Journal of Mushroom
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• v.15 no.4
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• pp.237-243
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• 2017

In order to develop fermented silkworm "Dongchunghacho" (Paecilomyces tenuipes) with improved absorption and increased effectiveness, we fermented Dongchunghacho using four kinds of microorganisms, viz., lactic acid bacteria, Bacillus subtilis, Natto bacillus, and yeast. A total of 15 samples were fermented using a combination of microbial inoculation culture and conditions to produce fermentation products. The contents of basic components such as sugar, reducing sugar, protein, total polyphenol, and total flavonoid were examined as well as the antioxidant, tyrosinase inhibitory, and thrombolytic activities of the fermented products were analyzed. We observed that reducing sugar and protein contents decreased in most of the fermented products, but the products fermented using yeast exhibited higher sugar content and, thus, higher sweetness. Total polyphenol content and antioxidant activity did not increase in fermented products compared to non-fermented Dongchunghachos, but total flavonoid content and tyrosinase inhibitory and thrombolytic activities increased by fermentation. In particular, total flavonoid content and tyrosinase inhibitory and thrombolytic activities primarily increased in the products fermented using yeast and lactic acid bacteria. However, it was not possible to confirm the increase in these activities in samples fermented by single fermentation using only yeast. Therefore, we propose that it will be possible to develop fermented food from silkworm Dongchunghacho (P. tenuipes) with excellent health benefits through additional study of multiple fermentation conditions using lactic acid bacteria and yeast.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.555-561
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• 2017

This study was conducted to investigate the several physicochemical extraction characteristics and the antioxidant activities of ethanol extract from Rhus javanica bark. The contents of soluble solid and sugar in extract was measured to 73.5 mg/100g dry basis and $17.8^{\circ}Brix$, respectively. The contents of total aromatics, total flavonoids and total phenolic compounds was measured to 0.508 in absorbance, 49.88 mg/100g and 296.6 mg/100g, respectively. The reducing power of extract was about 27.5 % of ascorbic acid with the same soluble solid contents of the extract. But the ferric reducing antioxidant power and the DPPH radical scavenging ability of extract were measured to equivalent to those of ascorbic acid. The metal ion chelating ability of the extract was 81.58 % whereas that of ascorbic acid was 74.73 %. The nitrite scavenging ability of the extract was measured to 51.76 % at pH 2. And the antioxidative effect of the extract on soybean oil was observed with Rancimat test.

• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.146-152
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• 2011

In the course of a study in relation to the production of taste, and functional enhancements in root crop chips, which were prepared by soaking dried yam slices in fruit juices, we investigated the physiological characteristics and biological activities of 8 different commercially available juices including; apple, omija (fruit of Maximowiczia typica), grape, wild grape, orange, tomato, red ginseng and black garlic juice. The average water contents, pH, brix and acidity of the juices used were $85.59{\pm}5.80%$, $3.90{\pm}0.64$, $12.19{\pm}4.70%$, and $0.49{\pm}0.19%$, respectively. The polyphenol content of black garlic and grape juice were 1.50 and 1.21 mg/ml, respectively, and those were higher than the average content (0.57 mg/mL) of the juices. Evaluation of anticoagulation activity showed that only omija juice has a strong thrombin inhibition, which is comparable to that of aspirin (1.5 mg/mL). Omija, grape and orange juice all exhibited antibacterial activity, but no antifungal activity. The 8 different juices, and in particular grape and black garlic juice, showed strong antioxidant activity in DPPH and ABTS radical scavenging activity assays, with wild grape juice demonstrating potent nitrite scavenging activity. These results suggest that omija, grape and black garlic juice can be used as soaking solutions to produce taste, and other functional enhancements, for root crop chips.

• Journal of Life Science
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• v.30 no.8
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• pp.688-694
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• 2020

In a previous study, the total phenolic content in ethanol extracts of medicinal plants that naturally grow on Jeju Island were analyzed with the extracts of Raphiolepsis indica leaf found to have the highest. The current study was carried out to evaluate the total flavonoid content, radical-scavenging activity, and the protective effect of R. indica extracts and solvent fractions on oxidative stress in HaCaT keratinocytes. More specifically, total flavonoid content and 2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging activity were measured to assess anti-oxidative activity, and protective effects against hydrogen peroxide (H₂O₂) were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay on the HaCaT cells. Of the various fractions analyzed, the ethyl acetate extract of R. indica showed the highest total flavonoid content (149.13 mg/g extracts) and the lowest remaining ABTS. In addition, the ethyl acetate fraction was significantly more resistant against H₂O₂ than the negative control. Our results therefore suggest that an ethyl acetate fraction of R. indica protects HaCaT cells against oxidative stress and could prove useful for developing functional cosmetic materials.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.215-224
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• 2010

A greenhouse experiment was conducted to determine the effects of medium components and shade treatment on the growth, contents of total phenolics and flavonoids, and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity of Youngia sonchifolia. Substrates combined with coco peat and perlite (ratio 70:30 or 50:50, v/v) showed higher plant length, leaf area, and fresh weight than single substrate (P<0.05). Shade treatment also significantly reduced plant height, root length, leaf areas, and fresh weight (P<0.05) with increasing of the degree. Shading treatment, however, increased contents of total phenolics [mg ferulic acid equivalents $kg^{-1}$ dry wt.] and total flavonoids [mg naringin equivalents $kg^{-1}$ dry wt.] in shoot parts of Y. sonchifoli, showing 110.2 to 119.2 and 128.3 to 146.7 mg $kg^{-1}$, respectively. The antioxidant potential of the methanol extracts from the plants dose-dependently increased DPPH free radical scavenging activity, and the activity was higher in shoots (50.2 to 80.8%) than in roots (47.7 to 49.8%), and in shading treatment than in no shade.

• Korean journal of food and cookery science
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• v.28 no.3
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• pp.275-283
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• 2012

This study was conducted to monitor the quality characteristics of Lespedeza cuneata ethanolic extracts, by a response surface methodology. The independent variables were the extraction temperature ($35{\sim}95^{\circ}C}$), extraction time (2~10 hr), and ethanol concentration (10~90%). The coefficients of the determinations (R2) were 0.8562 (p<0.1), 0.9787 (p<0.01), and 0.8344 (p<0.1) in total polyphenol, total flavonoid, and electron donating ability, respectively. The electron donating ability and nitrite scavenging effect were improved with an increase of ethanol concentration, rather than the extraction temperature. ORAC (Oxygen Radical Absorbance Capacity) with extraction conditions was 1,636.47~2,696.99 ${\mu}moles$ TE/g, and was increased with the increase of extraction temperature and 40~60% of ethanol concentration. Estimated conditions for the maximized extraction including the yield, total polyphenol, electron donating ability, nitrite scavenging effect, and ORAC, were $60{\sim}68^{\circ}C$ in extraction temperature, 6~7 hr in extraction time, and 38~60% in ethanol concentration.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.878-885
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• 2016

A study was conducted to examine the effects of light quality on the growth and phytochemical contents of ice plant in a closed-type plant production system. Seeds were sown in a 128-cell plug tray using rockwool. The seedlings were then transplanted into a deep floating technique system with recirculating nutrient solution (EC $1.5dS{\cdot}m^{-1}$, pH 6.5) in a closed-type plant production system. The nutrient solution was supplied at two weeks after transplanting with 2.0 mM NaCl concentration in all treatments for the development of the bladder cells. The three light sources with different light qualities used were as followed; FL (fluorescent lamps), combined RW LED (red:white = 7:3), and combined RBW LED (red:blue:white = 8:1:1) at $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with a photoperiod of 14/10 hours (light/dark). The results showed that the FL treatment had the greatest growth enhancement effects on the leaf area and the fresh and dry weights of the shoots and roots. The SPAD values were significantly higher under the FL and RBW LED treatments, at 29.8 and 30.6, respectively. No significant difference was observed in salinity under all treatments. Chlorophyll fluorescence was significantly higher under the FL treatment. The total phenol content and antioxidant activity were the highest under the RBW LED treatment. The total flavonoid content was significantly higher under the RBW LED and FL treatments. Hence, the results indicate that the growth of ice plant was maximized under the FL treatment. The phytochemical contents were maximized under the RBW LED treatment.

• Journal of agriculture & life science
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• v.46 no.6
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• pp.59-65
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• 2012

This study evaluated the baby-leaf growth, leaf red color intensity, and phytochemical compounds, such as total phenols and total flavonoids using domestic Amaranthus accessions, to select promising germplasm for the use of baby-leaf vegetable. The 39 accessions, upper 15% of 262 Amaranthus germplasm collection showing high red color intensity in baby-leaf, measured as Hunter's a value, were primarily selected. The eleven accessions including IT199970, showing stable red color intensity in baby-leaf both in high-temperature and low temperature cultivation, were finally selected as promising germplasm for the use of baby-leaf vegetable Amaranthus. The leaf red color intensities when they were cultivated at low temperature period were generally higher than those at low temperature period, and all the eleven selected germplasm, including four Amaranthus sub-species and five unknown species, showed stable red color expression in baby-leaf. The total phenol and total flavonoid contents were also presented for finally selected germplasm including their baby-leaf fresh weights. These finally selected germplasm could be potentially used as baby-leaf vegetable resources, through fortifying the color balance of salad vegetable.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.527-534
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• 2019

Japanese sumac (Rhus javanica) is one of the common herbaceous plants growing over the country. This study was conducted to investigate the extraction characteristics and physiological activities inluding nitrite scavenging ability of the water extracts from leaf, fruit and bark of Japanease sumac. Extraction yield was 6.62~13.84%, free amino acids were detected as 24 kinds with 37.9 mg/100g in leaf extract, 23 kinds with 27.0 mg/100g in fruit extract and 27 kinds with 39.0 mg/100g in bark extract, respectively, and seven kinds essential amino acids were detected. Total contents of flavonoids equivalent to naringin were 587.2 mg/100g in bark extract, 557.3 mg/100g in fruit extract and 379.9 mg/100g in leaf extract, respectively. Total contents of phenolics equivalent to gallic acid were 111.2 mg/100g in leaf extract, 108.4 mg/100g in fruit extract and 80.4 mg/100g in bark extract, respectively. The nitrite scavenging ability of extracts was order of 61.93% in bark extract>57.38% in fruit extract>55.49% in leaf extract, and was 78.1~100% of those of BHT (butylated hydroxytoluene) equivalents at pH 1.2. The electron donating ability was order of 47.38% in fruit extract>43.06% in leaf extract>38.55% in bark extract, and was compared to 65.6%, 58.8% and 53.6% of those of BHT equivalents, respectively. The reduction power was evaluated to 37% higher in leaf extract, 43% higher in fruit extract and 46% higher in bark extract than those of BHT equivalents. The metal chelating ability of extracts was considerably low and was order of 27.3% in bark extract>20.6% in leaf extract>11.2% in fruit extract.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.96-96
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• 2019

쌈용과 장아찌로 많이 이용하며 다양한 효능을 가진 곰취(Ligularia fischeri)의 신품종 중에 항산화 활성이 높은 것으로 밝혀진 '쌈마니' 품종에 대하여 좀 더 구제적인 항산화 활성을 알아보기 위하여 재배지역 및 수확시기별 항산화 성분 및 생리활성 정도를 비교 분석하여 지역별 적정 수확시기를 구명하고자 본 연구를 수행하였다. 시료는 2018년 4월 18일부터 2018년 7월 9일 까지 20일 간격으로 4회에 걸쳐 고랭지인 고령지농업연구소 대관령시험포장과 평난지인 강릉시험포장에서 수확하였으며 모두 동결 건조하여 분말형태로 갈아 실험에 사용하였다. 분석결과 '쌈마니' 품종의 지역 및 수확시기별 수율은 유사하였으며 총페놀 함량은 평난지에서는 5월 9일이 264.7mg GAE/g로 가장 높았고 그다음이 5월 29일, 6월 19일, 4월 18일 순이었다. 대체적으로 5월에 높았으며 고랭지에서도 초기 생산보다는 중기 생산기인 6월 18일에 총페놀 함량이 274.4mg GAE/g로 가장 높았고 그다음이 7월 9일, 5월 28일, 5월 10일 순이었다. 총플라보노이드 함량도 같은 경향으로 평난지에서는 5월 9일에 76.0mg QE/g로 가장 높았으며 그다음이 5월 29일, 6월 19일, 4월 18일 순이었으며 고랭지에서는 6월 18일에 66.5mg QE/g으로 가장 높았고 그다음이 7월 9일, 5월 28일, 5월 10일 순이었다. 재배지역 및 수확시기별 DPPH 활성산소 제거능에서도 평난지에서는 5월 9일과 5월 29일에 효과적이었으며 고랭지에서는 6월 18일에 효과가 있는 것으로 나타나 너무 이른 시기나 늦어지면 활성이 떨어지는 것으로 생각된다. 또한 환원력에 있어서도 평난지에서는 $1,000{\mu}g/m{\ell}$의 농도에서 5월 9일, 5월 29일, 6월 19일에 높은 경향을 나타냈으며 고랭지에서는 6월 18일에 높게 나타났다. 이러한 결과로 볼 때, 쌈마니 품종은 평난지에서는 5월, 고랭지에서는 6월에 수확하는 것이 항산화 활성이 높은 것으로 사료되었다.