• Korean Journal of Agricultural and Forest Meteorology
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• v.22 no.2
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• pp.57-67
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• 2020

Pinus densiflora is the most widely distributed tree species in South Korea. Its ecological and socio-cultural attributes makes it one of the most important tree species in S. Korea. In recent times however, the distribution of P. densiflora has been affected by dieback. This phenomenon has largely been attributed to climate change. This study was conducted to investigate the responses of growth and physiology of P. densiflora to drought and nitrogen fertiliz ation according to the RCP 8.5 scenario. A Temperature Gradient Chamber (TGC) and CO₂. Temperature Gradient Chamber (CTGC) were used to simulate climate change conditions. The treatments were established with temperature (control versus +3 and +5℃; aCeT) and CO₂ (control: aCaT versus x1.6 and x2.2; eCeT), watering(control versus drought), fertilization(control versus fertilized). Net photosynthesis (P_n), stomatal conductance (g_s), biomass and relative soil volumetric water content (VWC) were measured to examine physiological responses and growth. Relative soil VWC in aCeT significantly decreased after the onset of drought. P_n and g_s in both aCeT and eCeT with fertiliz ation were high before drought but decreased rapidly after 7 days under drought because nitrogen fertilization effect did not last long. The fastest mortality was 46 days in aCeT and the longest survival was 56 days in eCeT after the onset of drought. Total and partial biomass (leaf, stem and root) in both aCeT and eCeT with fertiliz ation were significantly high, but significantly low in aCeT. The results of the study are helpful in addressing P. densiflora vulnerability to climate change by highlighting physiological responses related to carbon allocation under differing simulated environmental stressors.

• Journal of Soil and Groundwater Environment
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• v.11 no.3
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• pp.43-51
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• 2006

Generally abandoned mine soils have serious problems for introducing vegetation such as nutrient deficiency, poor physical properties, and phytotoxicity due to high levels of heavy metals. It is required to improve soil amenity for revegetation. One of its strategies is using organic materials such as compost manure and sludge. The pot experiments was conducted to evaluate the effects of pig manure and municipal sewage sludge on revegetation of mining area soil surface with Artemisia princeps and Zoysia japonica. Application rate of pig manure and municipal sewage sludge was $75{\sim}225$ Mg/ha and $150{\sim}450$ Mg/ha, respectively. The results showed that the application of manure and sludge increased organic matter about two-fold and total nitrogen contents about five-fold of mine soil and improved the growth of plants in all treatments compared to the control. The result of plant tissue analysis showed that both plants accumulate Cd, Cu and Zn in root tissue rather than shoot tissues. Increased sludge application reduced Zn accumulation in both plant tissue. Sequential extraction results indicated that addition of soil amendment induced increment of organically bound fractions of Cu and Zn. Organically bound fraction of Zn was significantly increased from 7.84% to 13.58% in Artemisia princeps planted soil and from 7.84% to 14.16% in Zoysia japonica planted soil, thereby bioavailability of heavy metals was reduced. The results suggested that application of organic materials to mine soil can reduce phytotoxicity of heavy metals and be helpful in introducing successful revegetation.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.517-528
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• 2010

Rice seed maturation and germination involve drastic changes in water and nutrient transport, in which tonoplast aquaporins may play an important role. In the present study, gene expression profiles of 10 tonoplast intrinsic proteins (TIP) from rice were investigated by RT-PCR during seed development and germination. OsTIP3;1 and OsTIP3;2 were specifically expressed in mature seeds. Their transcript level rapidly decreased after onset of seed germination and gene expression was induced by ABA treatment. In contrast, expression of OsTIP2;1 and OsTIP4;3 was not seed specific as transcripts were found in vegetative tissues as well. Their respective transcript levels decreased at an early stage of seed development, whereas they increased at a later stage of seed germination and elongation of embryonic roots and shoots. When seed germination was inhibited by various stress conditions and ABA, expression of OsTIP2;1 and OsTIP4;3 was completely suppressed. In contrast, the expression level of OsTIP2;2 rapidly increased after seed imbibition and the transcript level was maintained under conditions inhibiting seed germination. These results implicate that tissue specific and developmental transcriptional regulation of OsTIPs in rice seeds depends on their specific function. In addition, OsTIPs can be discriminated by different potential phosphorylation and methylation sites in their protein structures. OsTIP3;1 and OsTIP3;2 possess unique phosphorylation signatures at their N-terminal domain, loop B and loop E, respectively. OsTIP2;1 and OsTIP4;3 have a potential methylation site at their Nterminal domain. This suggests that activity of specific tonoplast aquaporins may be regulated by post-translational modification as well as by transcriptional control.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.374-380
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• 2010

Well-feathered (5.2 feathers, stem diameter 13 mm) trees of 'Fuji' apple/ M.9 EMLA were planted at $4.0{\tiems}1.5$ m and whip trees (stem diameter 10 mm) of 'Fuji'/M.26 at $4.0{\times}2.0$ m were trained to the slender spindle. The productivity and yield efficiency of two orchard systems were compared for 6 years. The canopy volume of the tree/M.9 EMLA reached $2.07m^3$ in 2nd year and increased slowly to almost the targeted tree volume of $2.9m^3$ in 4th year. Trees/M.26 grew slowly at the begin but from 3rd year the tree volume expanded quickly to reach $5.6m^3$ in 5th year, covering over the allowed space. Yield of M.9 EMLA per 10a increased from 0.3 ton in 2nd year to 4.6 ton in 5th year, and 5.0 ton in 6th year, but yield of M.26 per 10a increased from 0.5 ton in 3rd to 2.9 ton in 6th year. Cumulative yield per 10a up to 6th year was 13.9 ton for M.9 EMLA but only 9.8 ton for M.26. Fruit weight for M.9 EMLA was heavier than that for M.26. In conclusion, the high density planting system with well-feathered trees/M.9 EMLA was better than the conventional wide planting system with whip trees/M.26.

• Horticultural Science & Technology
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• v.29 no.1
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• pp.16-22
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• 2011

In developing soil wetting agent using polyoxyethylene nonylphenyl ether (PNE) and polyoxyethylene castor oil (1:1; v/v), the effect of application rates on changes in concentration of PNE, initial wetting of peatmoss + perlite (7:3) medium, and growth of hot pepper (Capsicum annuum L. 'Knockwang') plug seedlings were investigated. The elevation of application rates of wetting agent increased the amount of water retained by the root media. The treatment of 2.5 $mL{\cdot}L^{-1}$ showed similar water retention to + control ($AquaGro^L$ 3.0 $mL{\cdot}L^{-1}$). Most of the liquid wetting agent (LWA) incorporated during the medium formulation leached out in the first and second irrigation, then it decreased gradually until 10 times in irrigation. In investigation of the influence of LWA on position of water infiltrating into root media, the vertical water movements in treatments of 0.5, 1.0, and 1.5 $mL{\cdot}L^{-1}$ were much faster than those in 0.0 $mL{\cdot}L^{-1}$ (-control), but relative speed of water movement decreased by the elevation in application rate of LWA to 2.0 or 2.5 $mL{\cdot}L^{-1}$. The evaporative water loss of root media that to contained various rate of LWA and irrigated to reach container capacity was the fastest in -control among the treatments and it delayed as the application rate of LWA was elevated. The plant height of 22.2 cm in 0.5 $mL{\cdot}L^{-1}$ and stem diameter of 3.26 mm in 1.0 $mL{\cdot}L^{-1}$ were the highest among the treatments tested. The treatment of 1.0 $mL{\cdot}L^{-1}$ also had the heaviest fresh and dry weights such among treatments tested as 3.08 g and 0.861 g per plant, respectively. The elevated application rate over than 1.5 $mL{\cdot}L^{-1}$ resulted in decreased seedling growth. The results mentioned above indicate that optimum application rate of LWA is 1.0 $mL{\cdot}L^{-1}$.

• Journal of agriculture & life science
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• v.43 no.2
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• pp.23-30
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• 2009

The object of this study was to evaluate the effect of the gibberellin treatment on fruit enlargement and ripening promotion in 'Niitaka' pear(Pyms pynfolia). Fruit weight was similar between $GA_{4+7}$ treatments and the gibberellin-paste control, but fairly increased of fruit weight compared to non-treatment Most effective time for $GA_{4+7}$ treatment to increase diameter and length was on 35 and 40 days after full bloom, respectively. Shape index was similar in all treatments. Fruit enlargement at the period of early growth, $GA_{4+7}$ 2.4% treatment was remarkably effective than $GA_{3}+GA_{4+7}$ 2.7% treatment or non-treatment However at the period of maturity, $GA_{4+7}$ 2.4% treatment and $GA_{3}+GA_{4+7}$ 2.7% treatment showed little differences in fruit enlargement and coloring. In maturing promotion effect, young fruit treated with $GA_{3}+GA_{4+7}$ showed similar fruit coloring to ethephon treatment on 35 days after full bloom, and both of those treatments promoted fruit coloring than non- treatment about 6 days. $GA_{3}+GA_{4+7}$ treatments resulted higher solid content and decreased acidity than non-treatment However, there was no differences in fruit hardness comparing to non-treatment As a result, gibberellin was most effective in fruit enlargement, so as all $GA_{3}+GA_{4+7}$ treatments were more effective on fruit enlargement than ethephon treatment or non-treatment Specifically, when $GA_{3}+GA_{4+7}$ was treated 35days after full bloom of flower, the diameter, the length and the shape of fruit index were best, and fruit coloring was good as well.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.4
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• pp.493-503
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• 2019

This study is composed of nine treatments [Control : "no neutralizing layer+vegetation layer" 3 cm, Treatment 1 : "no neutralizing layer+vegetation layer" 5 cm, Treatment 2 : "no neutralizing layer+vegetation layer" 7 cm, Treatment 3 :"neutralizing layer (cement 3 %)+ vegetation layer (cement 1 %)" 3 cm, Treatment 4 : "neutralizing layer (cement 3 %)+vegetation layer (cement 1 %)" 5 cm, Treatment 5 : "neutralizing layer (cement 3 %)+vegetation layer (cement 1 %)" 7 cm, Treatment 6 : "neutralizing layer [$(Ca{\cdot}Mg)CO_3$] +vegetation layer" 3 cm, Treatment 7 : "neutralizing layer [$(Ca{\cdot}Mg)CO_3$]+vegetation layer" 5 cm, Treatment 8 : "neutralizing layer [$(Ca{\cdot}Mg)CO_3$]+vegetation layer" 7 cm] to find out the vegetation effects according to neutralizing layer types of the acid drainage slope. There were no significant differences observed in soil hardness and soil moisture content of neutralizing layer type while highly difference of moisture content was observed according to the neutralizing and vegetation layer thickness. As for soil acidity, strong acid was shown in the control, treatment 1 and treatment 2. Neutralizing effects were outstanding in treatments of 3, 4, 5 (cement treatment group), 6, 7 and 8 (limestone treatment group). Concerning plants growth characteristics, surface coverage rates, number of germinating woody plants, plant height, and plant root status, there were excellent effects observed in the experimental groups mixed with cement (treatments 3, 4 and 5) and limestone (treatments 6, 7 and 8). At the initial stage, however, plant roots were negatively affected in cement layer treatments of 3, 4 and 5. However, no difference was shown in each layer thickness on the acid drainage slope whereas 3~5 cm thickness neutralizing layer was appropriate in consideration of economic feasibility.

• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.623-629
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• 2011

The detectable levels and population fluctuations of phytoplasmas infecting dwarf mulberry trees were investigated using nested-PCR and competitive-PCR methods. Samples of five different types were studied : A. petiole of a leaf that displays dwarf symptoms, B. petiole from apparently healthy leaf residing on a branch also supports a leaf with dwarf symptoms, C. the branch portion that supports a leaf with dwarf symptoms, D. the leaf petiole from healthy appearing leaves on branch with no dwarf symptoms, and branch portion of branch with no dwarf symptoms, E. the rootlets of trees with dwarf symptoms. These 5-parts were collected from each tree during June - April, once in every two months. The phytoplasma was detected from all parts of collected mulberry samples during all seasons using nested-PCR with AS-1/AS-2 primer pairs. The phytoplasma was detected until $10^4$ dilution using direct-PCR method, but it was detected until $10^{13}$ dilution by the nested-PCR method. The density of pytoplasma was found to be $7.94{\times}10^{18}-10^{12}copies/{\mu}L$ in mulberry trees. The density of phytoplasma was observed throughout the year in all samples of mulberry trees. The highest rates of phytoplasma was found in the samples B and C during the early growing season followed by the sample A and D during the dormant season. Samples C and E displayed the highest phytoplasma density followed sample D. The density of phytoplasma appeared stable during all the seasons for samples C and A. The result of the present study demonstrates the utility of nested-PCR and competitive-PCR for detection and determination of population fluctuations of phytoplasmas in plant tissues.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.37-71
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• 2021

Rapeseed is a typical winter crop, and its freezing stress tolerance is a major feature for winter survival. Therefore, it is important to comprehend clearly the physical and molecular mechanisms of rapeseed under freezing stress conditions. This study investigates the physical and transcriptome changes of two rapeseed lines, 'J8634-B-30' and 'EMS26', under cold acclimation and freezing temperature treatments. The proline content of 'J8634-B-30' at 5 ℃ increased 8.7-fold compared to that before treatment, and there was no significant change in that of 'EMS26' RNA-sequencing analysis revealed 5,083 differentially expressed genes (DEGs) of 'J8634-B-30' under cold acclimation condition. Among the genes, 2,784 (54.8%) were up-regulated and 2,299 (45.2%) were down-regulated. The DEGs of 'EMS26' under cold acclimation condition were 5,831 genes, and contained 2,199 up-regulated genes (37.7%) and 3,632 down-regulated genes (62.3%). Among them, only DEGs annotated in the cold response-related signaling pathways were selected, and their expression in the two rapeseed lines was compared. Comparative DEGs analysis indicated that cold response related signaling pathways are proline metabolism and ABA (Abscisic acid) signaling. And ICE (Inducer of CBF expression) - CBF (C-repeat-binding factor) - COR (Cold-regulated) signaling were the significantly differentially expressed transcripts in the two rapeseed lines. The major induced transcripts of 'J8634-B-30' induced P5CS (Δ'-pyrroline-5-carboxylate synthetase), which is related to proline biosynthesis, PYL (pyrabactin resistance-like protein, ABA receptor) and COR413 (cold-regulated 413 plasma membrane 1). In conclusion, these result provide a foundation for understanding the mechanisms of freezing stress tolerance in rapeseeds. Further functional studies should be performed on the freezing stress-related genes identified in this study, which can contribute to the transgenic and molecular breeding for freezing stress tolerance in rapeseed.

• Journal of Mushroom
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• v.18 no.4
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• pp.317-322
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• 2020

This experiment was conducted to solve the failure of fruiting body production in the bottle cultivation of the oyster mushroom cultivar 'Heuktari'. The effects of incubation temperature on primordium formation and fruiting body yield of the oyster mushroom cultivar 'Heuktari' were investigated. The proper temperature for mycelium growth of 'Heuktari' on potato dextrose agar (PDA) medium is 23-26℃. The mycelial growth of 'Heuktari' was faster than that of Chunchu 2ho. During mycelial culture in sawdust medium, the temperature of the medium in the bottle initially increased, reached the highest point in the middle of the culture, and then decreased. The higher the set temperature, the shorter the incubation period. When the incubation temperatures were 20℃ and 24℃, respectively, the undeveloped primordium formation rates were low (1.8% and 4.2%, respectively). However, the rate of undeveloped primordium formation increased, and the yield decreased at incubation temperatures of 16℃ and 28℃. Mushroom farms that set incubation temperatures to 18℃ and maintained the medium temperature at less than 28℃ showed undeveloped primordium formation rates ranging between 0.3-0.8%. The rate of undeveloped primordium formation increased and the yield decreased in the farms with high incubation temperatures (above 28℃). We found that in order to reduce undeveloped primordium formation, the air inside the incubation room should be circulated continuously so that the temperature of the medium does not rise above 28℃, and dense incubation conditions should be avoided.