• Title/Summary/Keyword: 초고압처리

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Changes in Microorganisms, Enzymes and Texture of Dongchimi by High Hydrostatic Pressure Treatment (초고압처리에 의한 동치미의 미생물, 효소 및 조직감의 변화)

  • Hong, Kwan-Pyo;Park, Ji-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.596-601
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    • 1998
  • Effects of high hydrostatic pressure on microorganisms, pectin enzymes and texture of dongchimi (pickled radish roots) were investigated. Dongchimi was pressurized at $200{\sim}686\;MPa$ for 5 min when its pH reached to 4.0. Total aerobes, initial concentration of $4.05{\times}10^8\;CFU/mL$, were completely inactivated by high hydrostatic pressure at 600 MPa. Lactic acid bacteria, yeasts and molds, initial concentration of $3.25{\times}10^8\;CFU/mL\;and\;3.55{\times}10^4\;CFU/mL$, respectively, were completely inactivated by high hydrostatic pressure at 400 MPa. Leuconostoc mesenteroides appeared to be the most barotolerant lactic acid bacteria because it was the sole bacteria survived at 380 MPa. Pectinesterase (PE) and polygalacturonase (PG) activities increased after high hydrostatic pressure treatment. Residual PE activity was 193 after pressurization at 500 MPa, and residual PG activity was 191 after pressurization at 686 MPa when the initial enzyme activity of control was set to 100. The hardness of pressurized dongchimi was higher than control.

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Effect of Addition of Phosvitin and High Pressure Processing on Microbiological Quality and Lipid and Protein Oxidation of Minced Chicken Leg Meat (닭 다리 분쇄육에 초고압 처리시 Phosvitin의 첨가가 미생물학적 품질과 지방 및 단백질 산화에 미치는 효과)

  • Jung, Samooel;Kang, Min-Gu;Kim, Il-Suk;Nam, Ki-Chang;Ahn, Dong-Uk;Jo, Cheo-Run
    • Food Science of Animal Resources
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    • v.32 no.2
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    • pp.212-219
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    • 2012
  • The objective of this study was to investigate the effect of high pressure (HP) processing on shelf life, as well as the addition of phosvitin on lipid and protein oxidation stability of minced chicken leg meat. Minced chicken leg meat was mixed with yolk phosvitin at 500 or 1000 mg/kg meat levels, and divided into raw and cooked groups. Then, the samples were subjected to HP at 0.1, 300, and 600 MPa. The total aerobic bacteria, lipid and protein oxidation, along with instrumental meat color ($L^*$, $a^*$, and $b^*$value) of the samples were measured during storage for 7 d at $4^{\circ}C$. In raw meat, the number of total aerobic bacteria was decreased by HP at 300 MPa (4 Log reductions) and 600 MPa (5 Log reductions) after 7 d of storage (p<0.05). HP at 600 MPa increased lipid oxidation of samples at all storage days and protein oxidation of samples during storage at 3 and 7 d. HP induced the changes of meat color by increase of $L^*$ value and decrease of $a^*$ value (p<0.05). The total aerobic bacteria was not detected in the cooked samples, regardless of HP pressure, and the lipid or protein oxidation of the cooked sample treated by 600 MPa was higher than that of the control (0.1 MPa) on day 7 or control on day 3, respectively (p<0.05). The results suggested that HP can improve the shelf life of minced chicken leg meat. However, phosvitin might be a limited antioxidative agent for the improvement of oxidation stability induced by HP.

Effects of High Pressure and Binding Agents on the Quality Characteristics of Restructured Pork (초고압처리 및 결착제 첨가가 재구성 돈육의 품질특성에 미치는 효과)

  • Choi, Ye-Chul;Jung, Kyung-Hun;Chun, Ji-Yeon;Choi, Mi-Jung;Hong, Geun-Pyo
    • Food Science of Animal Resources
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    • v.33 no.5
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    • pp.664-671
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    • 2013
  • The objective of this study was to investigate the effect of high pressure treatment and type of binding agents on the quality characteristics of restructured pork. For binding agents, 2% (w/w) isolated soy protein (SP), 0.5% (w/w) wheat flour (WF) and 0.5% (w/w) ${\kappa}$-carrageenan (KC) were incorporated into meat batter with or without 0.5% (w/w) glucono-${\delta}$-lactone (GdL). The restructured pork was pressurized at varying pressure levels (0.1-450 MPa) for 3 min under ambient temperature and thermal treated at $75^{\circ}C$ for 30 min. As quality parameters of restructured pork, pH, water binding properties, instrumental color and texture profile analysis were determined and compared with control (C, no binder). For type of binders, SP exhibited the best water binding properties, however, the impact on textural properties were lesser than KC and WF. The addition of GdL decreased the pH of restructured pork down to 0.4 unit, while high pressure processing prevented the moisture loss caused from pH decrease by GdL. In particular, meat restructuring efficiency of SP as a binder improved under the presence of GdL. Therefore, the present study demonstrated the potential advantages of low amount of GdL (0.5%, w/w) combined with protein based binder (SP) and high pressure processing in restructuring meat particles.

Quality Evaluation of Spent Hen and Broiler with Pulsed Electric Field and Super Heated Steam Treatment (펄스자기장 및 과열증기 처리를 통한 노계육과 육계의 품질 평가)

  • Jeon, Ki-Hong;Hwang, Yoon-Seon;Kim, Young-Boong;Choi, Yun-Sang;Kim, Eun-Mi;Lee, Dong-Un;Choi, Jinyoung
    • The Korean Journal of Food And Nutrition
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    • v.29 no.5
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    • pp.753-759
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    • 2016
  • The processing properties of spent hen and broiler chicken were investigated before and after treatment to improve texture characteristics. Each treatment consisted steaming (S) with $85^{\circ}C$ for 20 min, Pulsed Electric Field (PEF) with 1.5 KV/cm for 4 sec, and Super Heated Steam (SH) with an oven temp. of $300^{\circ}C$, a steam temp. of $350^{\circ}C$ for 8 min. The yield of spent hen and broiler were 66.85% and 63.80% respectively in the control, but decreased in every treatment was lowest at 61.05% in the PEF treatment (p<0.05). In the color test, L value decreased, but the a and b values increased regardless of the species of spent hen or broiler. In the test of heating loss, the S treatment of spent hen had the highest result of 45.25% but lowest of 30.66% in the SH treatment of the broiler. When it was compared with various treatments, SH after PEF treatment showed the better result in terms of heating loss than the PEF or SH treatment respectively. In the test of texture, the broiler showed the lowest hardness of 5.57 kg in the SH (p<0.05). Otherwise, the spent hen resulted in 14.08 kg of hardness in steaming after PEF, but it improved significantly to 10.73 kg in SH after PEF. In the test of 9 scored sensory evaluation of overall palatability, 7.8 point was the best score with SH treatment in the broiler. The best score in spent hen was 6.3 point which was SH after PEF treatment. With this experiment, SH after PEF was the condition in the treatments to have the better texture of spent hen.

Effect of Various Sterilization Methods on Growth of Microorganism Contaminated in Ginseng Powder (여러 가지 살균방법이 인삼분말에 오염된 미생물의 성장에 미치는 영향)

  • 곽이성;장진규
    • Journal of Food Hygiene and Safety
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    • v.16 no.3
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    • pp.221-226
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    • 2001
  • Various sterilization methods were applied to the powder of ginseng for the improving hygienic quality. Ultra-violet (UV) and Infrared ray (IR) treatments could not inhibit highly growth of bacteria in ginseng powder. However, high hydrostatic pressure treatment showed high inhibition rate against bacterial growth in ginseng powder. Changes of viable cell count by the pressure showed positive relationship between growth inhibition rates and the pressures applied. When powder was treated with 2,000 kg/$\textrm{cm}^2$ for 10 min at $25^{\circ}C$, initial viable cell count of the powder, 2.0$\times$10$^4$CFU/g, was decreased to 1.0$\times$10$^4$CFU/g. When it treated with 3,000, 4,000 and 5,000 kg/$\textrm{cm}^2$ of pressures under the same condition, viable cell counts were 8.0$\times$10$^3$, 7.0$\times$10$^3$and 1.8$\times$ 10$^3$CFU/g, respectively. Ginseng saponins of the powders were all detected when analyzed by TLC chromatography after treatment with the Pressures. Therefore, it was considered that saponin of ginseng powder was stable under the condition of 5,000 kg/$\textrm{cm}^2$ of pressure, even though the treatment induced coagulation of the powder.

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Inactivation of Microorganisms and Enzymes in Foxtail Millet Yakju by High Hydrostatic Pressure Treatment (초고압 처리에 의한 좁쌀약주의 미생물 살균 및 효소 불활성화)

  • 좌미경;임상빈;목철균;박영서
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1221-1226
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    • 2003
  • High hydrostatic Pressure was applied to Foxtail Millet Yakju to investigate the effects of high pressure on inactivation of microorganisms and enzymes. Total bacteria, lactic acid bacteria and yeast in untreated Yakju were $1.5{\times}$10$^4$,1.9${\times}$10$^4$ and 1.4${\times}$10$^4$ CFU/mL, respectively. Total bacterial count was reduced to 4.1${\times}$l0$^2$ CFU/mL, while lactic acid bacteria and yeast were sterilized completely in Yakju heated at $65^{\circ}C$ for 15 min. Lactic acid bacteria and yeast decreased greatly with the increase of treatment pressure, and were sterilized completely in Yakju treated at more than 300 ㎫ for 10 min/$25^{\circ}C$. Total bacteria were not completely sterilized with pressurization of even 600 ㎫ at room temperature and reduced to 2 log cycle even at $65^{\circ}C$. Total bacteria decreased by 2∼3 log cycle with the increase of treatment time from 10 to 60 min at $25^{\circ}C$/300 ㎫. Pressurization of Yakju caused a partial inactivation of $\alpha$ -amylase and glucosamylase, and the activities of $\alpha$ -amylase and glucoamylase decreased by 18.1% and 21.1%, respectively at $25^{\circ}C$/600 ㎫/10 min. Activities of $\alpha$ -amylase and glucoamylase decreased with the increase of temperature, and 22.2% and 32.1% of the original activity were remained with the treatment at $65^{\circ}C$/300 ㎫/10 min, respectively. Enzyme activities decreased slightly with the increase of treatment time at $65^{\circ}C$/300 ㎫.

Effect of High Hydrostatic Pressure Treatment on Extension of Shelf Life of Draft Makgeolli (초고압처리가 생막걸리의 저장기간 연장에 미치는 영향)

  • Choi, H.S.;Kang, C.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.22 no.2
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    • pp.5-15
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    • 2020
  • To extend the shelf life of draft makgeolli, UV irradiation and high hydrostatic pressure were conducted. UV irradiation did not reduce the total cell number in makgeolli, but high hydrostatic pressure treated drastically decreased. The number of viable cells in makgeolli was 2.2-5.8 × 107 CFU/mL, but when treated with 300 MPa pressure for 1 minute, it decreased to 1.4-10 × 103 CFU/mL, with 400 MPa to 4-68 CFU/mL, and with 500 MPa to under 40 CFU/mL. Yeast died at 400 MPa pressure, but Bacillus amyloliquefaciens and Rummeliibacillus stabekisii survived at 500 MPa pressure. The makgeolli treated with 400 and 500 MPa pressure did not increase the number of cells even when stored at room temperature (25℃) for 30 days, and changes in alcohol, acidity, and amino acid vlaue were also suppressed. However, when stored for more than 30 days at room temperature, the acidity and amino acid levels of makgeolli increased, making it difficult to drink. At low temperature (4℃) storage, the quality change of makgeolli was suppressed until 70 days of storage, so it had a taste similar to that of fresh makgeolli.

Study on the Hurdle Technique for the Reduction of Bacillus cereus Spores in Doenjang and Gochujang (장류에 접종된 Bacillus cereus 포자의 저감화 기법에 관한 연구)

  • Lee, Nam-Hyouck;Jo, Eun-Ji;Oh, Se-Wook;Hong, Sang-Pil
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.12
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    • pp.1842-1846
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    • 2012
  • The effects of hurdle techniques on the reduction of Bacillus cereus spores in Doenjang and Gochujang were investigated. In our system, Bacillus cereus spores were artificially inoculated into Doenjang and Gochujang. Hurdle techniques used in this study were additives (3% ethyl alcohol-0.03% oregano extract), Joule heating ($95^{\circ}C$ for 5 min), and hydrostatic pressure (500 MPa for 5 min at $45^{\circ}C$). Additive-Joule (AJ) and additive-Joule-pressure (AJP) treatments for Doenjang resulted in a 2.80 log and 3.74 log reduction, respectively, while treatments for Gochujang resulted in a 4.71 log and 5.60 log reduction, respectively. This suggests a high synergistic effect of Joule heating with additive treatment in Doenjang and Gochujang. A combination ofg hurdles such as additives, Joule heating, and hydrostatic pressure also kept Bacillus cereus spore counts low during storage at $30^{\circ}C$. Therefore, Bacillus cereus spores inoculated into Doenjang and Gochujang can be effectively reduced through combined treatments, including AJ or AJP.

Skin-Whitening and UV-Protective Effects of Angelica gigas Nakai Extracts on Ultra High Pressure Extraction Process (초고압 추출 공정에 의한 당귀 추출물의 미백 및 자외선 차단 효과)

  • Kim, Cheol-Hee;Kwon, Min-Chul;Han, Jae-Gun;Na, Chun-Su;Kwak, Hyeong-Geun;Choi, Geun-Pyo;Park, Uk-Yeon;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.4
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    • pp.255-260
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    • 2008
  • This study was performed to investigate the enhancement of UV-protection activities and skin-whitening effects from Angelica gigas Nakai extracts on ultra high pressure extraction process. Extraction at $60^{\circ}C$ treated by ultra high pressure for 15 minute and associated with ultrasofication (HPE15) was showed more than double yield, compare conventional extraction, as 12.24% (w/w) from A. gigas. Extracts of HPE15 reduced expression of MMP-1 on UV-irradiated CCD-986sk cells as 122.2% and revealed high inhibitory potency on tyrosinase as 69.4% by adding samples. Extracts of HPE15 from A. gigas showed strong inhibition effect on melanin production test by Clone M-3 cells as 82.4% by adding extracts. From the preliminary observations, we considered that the extracts from A. gigas could be potent natural materials for skin-whitening agent, and could be used as a potential anti-aging agent for the photo-damaged skin.