• Title/Summary/Keyword: 청정도 실험

Search Result 518, Processing Time 0.024 seconds

Study on Characteristics of Antibody from Milk Immunized with Some Helicobacter pylori Antigen (Helicobacter pylori 항원을 이용한 면역우유의 항체특성에 관한 연구)

  • Park, Chang-Ho;Ye, Eun-Ju;Kim, Soo-Jung;Bae, Man-Jong
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.34 no.5
    • /
    • pp.619-625
    • /
    • 2005
  • This study has been carried out to examine the characteristics of anti-H. pylori antibodies of milk produced from cows immunized with antigen of Helicobacter pylori such as peculiarity of antigen antibody, agglutination of H. pylori strain, stability of antibody against acid, alkali and heat treatments. The molecular weight of anti-H. pylori antibody measured by SDS-PAGE were turned out as about 50 kDa in the heavy chain and about 24 kDa in the light chain. Twenty protein bands were visualized in H. pylori interacting with anti-H. pylori antibody which was made in dairy cow by immunization with H. pylori. The western blotting was peformed in order to examine the antigen peculiarity of anti-H. pylori, The results were all 7 antigen substances including serum, furified serum, whey and furified whey could be confirmed and the major antigen substances were 97, 66,34 kDa of molecular weight. As a result of agglutination response anti-H. pylori antibody in whey showed 1/10 agglutination value against H. pylori. In stability test about acid and alkali of antibody, there was $100\%$ activated at the range of $pH5\~pH10$. In stability test about heat, the antibody showed stable condition at $60^{\circ}C$ for 60 minutes and comparatively stable condition at $70^{\circ}C$, but reduced activation to $40\%$ after 60 minutes. It maintained $77\%$ activation at $80^{\circ}C$ for 4 minutes and comparatively stable at $100^{\circ}C$ for 1 minute.

Effects of chewable tablet containing sodium fluoride and xylitol on the oral hygiene state in the orthodontic patients (자일리톨과 불화나트륨을 함유한 저작성 정제가 교정환자의 구강위생에 미치는 영향에 관한 임상시험)

  • Yu, Sang-Hoon;Ahn, Sug-Joon;Lee, Shin-Jae;Baek, Seung-Hak;Kim, Tae-Woo;Chang, Young-Il;Nahm, Dong-Seok
    • The korean journal of orthodontics
    • /
    • v.33 no.2 s.97
    • /
    • pp.113-120
    • /
    • 2003
  • The aim of this study was to evaluate the effect a chewable tablet containing sodium fluoride and xylitol (DenPo, Hamizzle, Bucheon, Kyungki) on the oral hygiene state in the orthodontic patients.'rho subjects consisted of 30 adult orthodontic patients, who have worn the fixed orthodontic appliance at least 6 months. They were instructed to use the tablet forth a day, Gingival index (GI), plaque index (PI), and sulcus bleeding index (SBI) of upper and lower anterior and posterior regions were measured before using the tablet (T0), 2 weeks after using the tablet (T1), and 4 weeks after using the tablet (T2). The data were analyzed with Wilcoxon signed ranks tests. The results were as follows ; 1 PI was decreased significantly in the T1 and T2 stage compared with T0 stage in the both anterior and posterior regions (p<0.05). Although PI was decreased in the T2 stare compared with T1, there was not statistical significance (p>0.05). 2. GI and SBI showed similar results compared with PI. In the T1 and T2 stage, there was statistically significant decrease in the GI and SBI (p<0.05), This study showed that the DenPo tablet could be used as an adjunct to improve oral hygiene in the orthodontic patients. The decrease of the indices nay suggest that DenPo tablet could be effective in the plaque removal and prevention of gngival inflammation in the orthodontic patients.

Preliminary Study on Desizing and Dyeing Using Low Temperature Plasma (저온 플라즈마를 이용한 호발 및 염색 선행연구)

  • Bae, Paek-Hyun;Jo, Hun-Je;Kim, Hyo-Jin;Lee, Yeon-Hee;Park, Sung-Min;Kim, Jeong-Gyu;Jung, Jin-Ho
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.27 no.9
    • /
    • pp.946-951
    • /
    • 2005
  • In this wort plasma treatment was evaluated as an alternative clean desizing technology. Size materials such as PVA(polyvinyl alcohol), PACL(polyacrylic acid esters) and their mixture on PET(polyethylene terephthalate) fabrics were treated by $N_2$ and $O_2$ plasma. $O_2$ plasma was more efficient in size removal than $N_2$ plasma, and the removal of PVA was higher than that of PACL. SEM(scanning emission microscopy) pictures of the plasma treated samples directly proved the disappearance of sizing agents. After $O_2$ plasma treatment, the PET fabrics were subjected to conventional desizing process. Compared with untreated fabrics, the desizing effluent from the treated fabrics gave lower TOC, COD and $BOD_5$ values. This indicates plasma treatment not only serves to directly remove sizing agents but also offered several advantages by changing the chemical properties of sizing agents. Lastly, the effect of plasma desizing process on dyeing was examined using color difference and dyeing fastness tests. The CCM(computer color matching) results showed rotor difference between PET fabric desized by $O_2$ plasma treatment for 20 min and reference PET fabric desized by the conventional wet desizing process was around 1. This suggests the treated PET fabric can be directly subjected to dyeing process without any additional process. The plasma treated fabric also gave a good result of dyeing fastness so that grades of laundering, crocking, heat and light fastness were same or even better than the reference PET fabric did.

Development of Overhead Projector Films, CD-ROM, and Bio-Cosmos Home Page as Teaching Resources for High School Biology (고교 생물의 오버헤드 프로젝터용 필름 제작 및 전달 매체로서의 CD-ROM과 홈페이지의 설계)

  • Song, Bang-Ho;Sin, Youn-Uk;Choi, Mie-Sook;Park, Chang-Bo;Ahn, Na-Young;Kang, Jae-Seuk;Kim, Jeung-Hyun;Seo, Hae-Ae;Kwon, Duck-Kee;Sohn, Jong-Kyung;Chung, Hwa-Sook;Yang, Hong-Jun;Park, Sung-Ho
    • Journal of The Korean Association For Science Education
    • /
    • v.19 no.3
    • /
    • pp.428-440
    • /
    • 1999
  • The colorful overhead projector films, named as Bio-cosmos II, including photographs, pictures, concept maps, and diagrams, were developed and manufactured as audio-visual teaching aids and teaching resources for students' biology learning in high school, and the CD-ROM and web sites for their application to the school were also constructed. The content of the films was organized based upon the analysis of seven different biology textbooks approved by the Ministry of Education. The films were designated based on various instructional strategies and manufactured using multimedia with various educational softwares. The CD-ROM was composed of the scenes as logo, initial main, chapters list, contents, and quit. Initial main scene indicated various chapters according to the texts of biology areas in General Science, Biology I, and II. Each chapters linked with the scenes for detailed concept maps, the downstream real subjects, and contents. The subject screens were composed of various types of summarized diagrams including lesson contents, figures, pictures, photographs, and their explanation, experimental procedures and results, tables for summarized contents, and additional animation with video captures, explanations, glossary, etc. Most files were manufactured in software Adobe Photoshop by scanning the pictures, figures and photographs, and then the explanation, modification, storing with PICT or PSD files, and transformation with JPG files, were processed in the aspect of high quality in terms of instructional strategies and graphic skills on gracefulness, clearness, colorfulness, brightness, and distinctness. A 14 films for biology areas in General Science, 80 for Biology I, and 142 for Biology II were manufactured and loaded to the CD-ROM and web site, and the files had been attempted to opened with an internet home-page of http://gic.kyungpook.ac.kr/biocosmos.

  • PDF

Bioactive Materials and Biological Activity in the Extracts of Leaf, Stem Mixture and Root from Angelica gigas Nakai (참당귀 잎, 줄기혼합물과 뿌리 추출물의 생리활성물질 및 그 활성작용)

  • Heo, Jin-Sun;Cha, Jae-Young;Kim, Hyun-Woo;Ahn, Hee-Young;Eom, Kyung-Eun;Heo, Su-Jin;Cho, Young-Su
    • Journal of Life Science
    • /
    • v.20 no.5
    • /
    • pp.750-759
    • /
    • 2010
  • The bioactive materials (phenolic compounds, flavonoids, minerals, decursin and decursinol angelate) and biological activities (DPPH [$\alpha,\alpha$'-diphenyl-$\beta$-picrylhydrazyl] free radical scavenging capability, reducing power, and tyrosinase activity) in the extracts of leaf, stem mixture (AGLS), and root (AGR) from Angelica gigas Nakai were examined by using water, hot water and ethanol solvent. The highest extract yield (21.89%) was found in the water extract of AGR. The highest concentrations of phenolic compounds and flavonoids in the ethanol extracts of AGLS and AGR were 14.99% and 14.79%. Major minerals of AGLS and AGR were K, Mg, Fe, Na and Ca. Decursin and decursinol angelate were the major ingredients of Angelica gigas, detected at 18.71 and 18.89 min of retention time by HPLC analysis, respectively. The highest concentrations of decursin and decursinol angelate in the Angelica gigas ethanol extract were found in root ($41.7\;{\mu}g/g$) and leaf ($34.04\;{\mu}g/g$). The highest free radical scavenging activity was found in the hot water extracts of AGLS and AGR, and its activity was stronger in all extracts of AGLS than AGR. The highest reducing power was found in the ethanol extracts of AGLS and AGR and this was dependent on the sample concentration. The hot water extracts of AGLS and AGR revealed the highest inhibition activity on tyrosinase. Overall, these results may provide the basic data needed to understand the biological activities of bioactive materials derived from Angelica gigas.

Microbial Hazard Analysis of Manufacturing Processes for Starch Noodle (당면의 제조공정별 미생물학적 위해요소 분석)

  • Cheon, Jin-Young;Yang, Ji Hye;Kim, Min Jeong;Lee, Su-Mi;Cha, Myeonghwa;Park, Ki-Hwan;Ryu, Kyung
    • Journal of Food Hygiene and Safety
    • /
    • v.27 no.4
    • /
    • pp.420-426
    • /
    • 2012
  • The purpose of this study was to identify control points through microbiological hazard analysis in the manufacturing processes of starch noodles. Samples were collected from the ingredients, manufacturing processes, equipment and environment. Microbiological hazard assessments were performed using aerobic plate counts (APC), Enterobacteriaceae (EB), E. coli and five pathogens including B. cereus, E. coli O157:H7, L. monocytogenes, Salmonella spp., and S. aureus. The APC levels in raw materials were from 2.12 to 3.83 log CFU/g. The contamination levels after kneading were 4.31 log CFU/g for APCs and 2.88 log CFU/g for EB counts. APCs decreased to 1.63 log CFU/g and EB were not detected after gelatinization, but their levels slightly increased upon cooling, cutting, ripening, freezing, thawing, and separating. The reuse of cooling and coating water would be a critical source of microbial increase after cooling. After drying, APCs and EB counts decreased to 5.05 log CFU/g and 2.74 log CFU/g, respectively, and the levels were maintained to final products. These results suggest that the cooling process is a critical control point for microbiological safety, and the cooling water should be treated and controlled to prevent cross contamination by pre-requisite program.

The Characteristics Study of Vehicle Evaporative Emission and Performance according to the Bio-Fuel Application (바이오 연료 적용에 따른 차량 증발가스 및 성능특성 연구)

  • Noh, Kyeong-Ha;Lee, Min-Ho;Kim, Ki-Ho;Kim, Sin;Park, Cheon-Kyu
    • Journal of the Korean Applied Science and Technology
    • /
    • v.34 no.4
    • /
    • pp.874-882
    • /
    • 2017
  • As the interest on the air-pollution is gradually rising up at home and abroad, automotiv e and fuel researchers have been working on the exhaust emission reduction from vehicles through a lot of approaches, which consist of new engine design, innovative after-treatment systems, using clean (eco-friendly alternative) fuels and fuel quality improvement. This research has brought forward three main issues : evaporative, performance, air pollution. In addition, researcher studied the environment problems of the bio-ethanol, bio-butanol, bio-ETBE (Ethyl Tertiary Butyl Ether), MTBE (Methyl Tert iary Butyl Ether) fuel contained in the fuel as octane number improver. The researchers have many dat a about the health effects of ingestion of octane number improver. However, the data support the con clusion that octane number improver is a potential human carcinogen at high doses. Based on the bio-fuel and octane number improver types (bio-ethanol, bio-butanol, bio-ETBE, MTBE), this paper dis cussed the influence of gasoline fuel properties on the evaporative emission characteristics. Also, this p aper assessed the acceleration and power performance of gasoline vehicle for the bio-fuel property. As a result of the experiment, it was found that all the test fuels meet the domestic exhaust gas standards, and as a result of measurement of the vapor pressure of the test fuels, the bio - ethanol : 15 kPa and the biobutanol : 1.6 kPa. thus when manufacturing E3 fuel, Increasing the biobutanol content reduces evaporation gas and vapor pressure. In addition, Similar accelerating and powering performance was shown for the type of biofuel and when bio-butanol and bio-ethanol were compared accelerated perf ormance was improved by about 3.9% and vehicle power by 0.8%.

Analysis of Ingredients and DPPH, ABTS Activity for the Development of Cosmetic Raw Materials using 5 Kinds of Plants Native to Mt. Jiri (지리산 자생식물 5종의 화장품 원료개발을 위한 성분 및 DPPH, ABTS 활성분석)

  • Youn Ok, Jung;Bo Kyung, Kang;No Bok, Park
    • Journal of Practical Agriculture & Fisheries Research
    • /
    • v.24 no.4
    • /
    • pp.18-29
    • /
    • 2022
  • Five species of plants (Clerodendrum trichotomum Thunb., Angelica dahurica (Fisch. ex Hoffm.) Benth. & Hook. f. ex Franch. & Sav., Caryopteris incana (Thunb. ex Houtt.) Miq., Lonicera japonica Thunb., and Parasenecio auriculatus var. matsumurana Nakai) native to the clean area of Mt. Jiri were selected. The collection period was from May to September 2021, and the five species plants were collected in their native habitats with flowers in full bloom. The collected plants were extracted with 70% EtOH, and 17 kinds of polyphenol components were analyzed. Next, flowers, leaves, stems, and roots were separated from plants, extracted with 70% EtOH for each part and experiments were conducted on DPPH, ABTS, total polyphenols, and total flavonoids. The results are as follows. 1. It was found that there were a total of 8 kinds of polyphenols contained in 5 species of plants that are native to Mt. Jiri. Among the polyphenol components, chlorogenic acid was contained in 4 species of plants, and caffeic acid was contained in 2 species of plants. 2. As a result, the DPPH radical scavenging activity was the best in the stem of P. auriculata and the C. trichotomum was good regardless of the specific part. It was found that the activity-scavenging activity was good in the flowers of A. dahurica and the leaves of L. japonica. 3. The highest ABTS radical scavenging activity was C. trichotomum Thunb., whose EC50 value was 38.73~66.28ppm. Next, the leaves and stems of L. japonica Thunb., A. dahurica and P. auriculata, and the leaves and stems of C. incana appeared in that order. 4. The highest total polyphenol content was 154.83mg GAE/g in the leaves of C. trichotomum, followed by about 130mg GAE/g in the flowers of C. trichotomum and P. auriculata. The lowest was 26.27mg GAE/g in the stems of A. dahurica.