• Journal of Chest Surgery
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• v.41 no.5
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• pp.550-562
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• 2008

Background: We attempted to reproduce a previously reported method that is known to be effective for decellularization, and we sought to find the optimal condition for decellularization by introducing some modifications to this method. Material and Method: Porcine semilunar valves, arterial walls and pericardium were processed for decellularization with using a variety of combinations and concentrations of decellularizing agents under different conditions of temperature, osmolarity and incubation time. The degree of decellularization and the preservation of the extracellular matrix were evaluated by staining with hematoxylin and eosin and with alpha-Gal and DAPI in some of the decellularized tissues. Result: Decellularization was achieved in the specimens that were treated with sodium deoxycholate, sodium dodesyl sulfate, Triton X-100 and sodium dodesyl sulfate with Triton X-100 as single-step methods, and this was also achieved in the specimens that were treated with hypotonic solution ${\rightarrow}$ Triton X-100 ${\rightarrow}$ sodium dodesyl sulfate, sodium deoxycholate ${\rightarrow}$ hypotonic solution ${\rightarrow}$ sodium dodesyl sulfate, and hypotonic solution sodium dodesyl sulfate as multi-step methods. Conclusion: Considering the number and the amount of the chemicals that were used, the incubation time and the degree of damage to the extracellular matrix, a single-step method with sodium dodesyl sulfate and Triton X-100 and a multi-step method with hypotonic solution followed by sodium dodesyl sulfate were both relatively optimal methods for decellularization in this study.

• Applied Biological Chemistry
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• v.18 no.3
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• pp.123-144
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• 1975

This study has been carried out to investigate the translocation-illuviation status of iron and manganese, which are striking phenomena in paddy soils, in relation to its morphological characteristics, and to find out a method to identify illuvial layer of iron quantitatively. Determination of active iron and easily reducible manganese content in surface soils of lowland paddy (266 samples) in Korea were conducted. The examination has been made on relationship between morphological, physico-chemical properties of the representative paddy soils (9 series) and iron and manganese content of their horizons. The results are summarized as follows. 1. The poorer the drainage, the higher concentration of active iron and easily reducible manganese were found, and under same drainage condition, the more the sand, the lower the content of them. 2. Irrespective of soil texture and drainage, highly signignificant positive correlation was found between the contents of active iron ($\hat{Y}$) and clay plus silt in surface soils. $$\hat{Y}=0.3929+(0.05352\;X\;clay%)+(0.0001023\;X\;silt%){\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;I$$ No correlation, however, was found between clay content and easily reducible manganese concentration. 3. Significant positive correlation was obtained between active iron ($\hat{Y}$) and total iron (x) content in each profiles of all soil series. Obtained regression equation is as follows; $$\hat{Y}=0.361x-0.480(r=0.651^{**}){\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;{\ldots}\;II$$ On the other hand, easily reducible manganese concentration had a tendency to increase, not significantly, with increasing total manganese concentration. 4. Accumulation of iron and manganese generally can be found in paddy soils, but distinct accumulation was found under moderately well drained fine loamy and clay soils, while surface accumulation occurred under poor drainage without regard to soil texture. 5. Profile description or determination of active iron in each horizon were found to be insufficient to designate illuvial layer of iron. Therefore, identification of illuvial layer of iron based on the ratio of total iron and active iron, and concentration of active iron estimated by the content of clay plus silt (Equation 1 above) was thought to be reasonable. Also, manganese accumulation layer would be estimated by total manganese and easily reducible manganese content and their ratio.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.2
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• pp.141-149
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• 1993

The seasonal variation of lipid content was mostly attributed to diet, water temperature and period of reproduction etc. The lipid mainly consisted of neutral lipid and phospholipid. Nonpolar lipid content was higher in between late spring and summer, while polar lipid content lower during the period. The composition of neutral, glyco, and phospholipid of the total lipid: in average was 53.88, 10.09 and $36.03\%$, respectively. The neutral lipids were composed of triglycride($51.88\%$) and free sterol($23.21\%$) as major component and a little quantity of diglycerides, monoglycerides, esterified sterols and hydrocarbon, free fatty acid were also identified. The phospholipids of each fraction were mainly occupied by phosphatidyl choline($55.5\%$), followed by phosphatidyl ethanolamine($27.8\%$), phosphatidyl inositol($8.65\%$) and phosphatidyl serine($4.85\%$). The major fatty acids of the total lipid in ascidian were $C_{20:5},\;C_{22:6},\;C_{16:6}\;and\;C_{18:1}$, respectively. The fatty acid composition of phospholipid and neutral lipid showed a similar tendency to that of the total lipid. The major fatty acids in the fraction of glycolipid, however, appeared $C_{16:0},\;C_{20:1},\;C_{18:0}\;and\;C_{18:1}$ in order.

• Journal of Chest Surgery
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• v.42 no.4
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• pp.418-425
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• 2009

Background: Cardiopulmonary bypass (CPB) involves use of an initial priming volume which can cause side effects such as hemodilution, transfusion, inflammatory reaction and edema. Hence, there have been efforts made tore-duce the initial priming volume. We compared this traditional method to a CPB method that uses a minimized priming volume (MPV). Material and Method: For 97 patients who underwent congenital cardiac surgery between July 2007 to June 2008, we discussed each case and decided which method to use. We reviewed the medical records and cardiopulmonary bypass sheets of the patients. Result: We used a MPV method for 46 patients, and a traditional method for the other 51. There were no significant differences in preoperative and intraoperative characteristics between the two groups, such as body weight, age, cardiopulmonary bypass time, lowest body temperature, etc. However, the priming volume was much smaller in the MPV group than the traditional group (p<0.001). The volume of initially mixed packed RBC was also much smaller in the MPV group (p<0.001). There were no significant differences in postoperative mortality and neurologic complications. Conclusion: We could significantly reduce the initial priming volume and initially mixed pRBC volume with the revised CPB method. We suggest that this method be used more widely for congenital cardiac surgery.

• Journal of dental hygiene science
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• v.16 no.4
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• pp.272-283
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• 2016

This study compared and analyzed the occluding effects of fluoride compounds and desensitizers, which are commonly used in dental clinics, on dentinal tubules. This study also evaluated the persistence of the active ingredients over time by performing toothbrushing with an electric toothbrush. Thirty-five molar teeth, which had been extracted within the past 3 months from healthy people without tooth decays, amalgam fillings, or dental crowns, were divided into 4 pieces each. Of these, 135 teeth pieces were used as study specimens. These specimens were divided into a control group, an untreated group, and 5 experimental groups (acidulated fluoride gel, fluoride varnish, Gluma, Super Seal, and SE-Bond). The specimens were then subjected to toothbrushing equivalent to 1 week (140 times), 2 weeks (280 times), and 4 weeks (560 times), and the occluding effects on dentinal tubules in 3 regions of each specimen were examined under a scanning electron microscope. The fluoride varnish treated group showed the highest degree of dentinal tubule occlusion effects during the first, second, and fourth weeks of toothbrushing, with the SE-Bond treated group showing the second highest degree and the Gluma treated group showing the lowest degree. After 4 weeks of toothbrushing, the Gluma treated group and the Super Seal treated group showed the lowest degrees of dentinal tubule occlusion effects. In summary, the fluoride varnish treated group and the SE-Bond treated group displayed higher occlusion effects even after 4 weeks of treatment than did the other experimental groups. Therefore, it is the authors' belief that fluoride varnish and SE-Bond are effective for treating dentinal hyperesthesia.

• Journal of dental hygiene science
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• v.16 no.3
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• pp.235-241
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• 2016

This study was conducted to provide basic understanding regarding possible enamel erosion by three kinds of fist-aid antipyretic and analgesic medicines over a period of time, with comparison and analysis of the resulting deciduous teeth surface and microhardness changes. The analysis was performed using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscope (SEM) to examine the surface erosion and changes. The Kruskal-Wallis test show differences in surface erosion and changes after 3, 5 and 8 days of treatment as well as before and after the treatment in each group. According to the results, there was no significant difference in the early deciduous teeth enamel surface microhardness (p>0.01). However there were signigicant changes after 3, 5, and 8 days (p<0.01). Calcim (Ca) and phosphorous (P) analysis using EDX showed significant differences in the enamel characteristics according to each tissue area after 8 days (p<0.05), but there was no significant difference in any of the areas for P content (p>0.05). In the surface observation with the SEM treatment with Children's Tylenol$^{(R)}$ tablet, which has the lowest pH, looked the roughest, followed by Brufen syrup for children and Children's Tylenol$^{(R)}$ suspension. Based on these results, it should be considered that antipyretic and analgesic medicines for children, which have lower pH values, may cause tooth erosion. Hence, it is necessary to give special attention to oral hygiene in young children or infants by brushing their teeth after such drugs are administered.

• Journal of the Korean Society of Groundwater Environment
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• v.6 no.2
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• pp.59-65
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• 1999

Both the groundwater changes due to different pumping rates and the geochemistry of thermal waters in the Suanbo area are considered in this study. The observation of groundwater level change since 1991 shows that the change is directly correlated with pumping rates of thermal waters and reveals the retardation of ca. 5 weeks after pumping. The hydrogeological aquifer in the area is under reducing condition. The thermal waters are of Na-HCO$_3$ type. and are alkaline (pH=8.5∼8.7) with low TDS values (274∼284 mg/l) and high concentrations of Na (68∼72 mg/l). F (6.4∼8.9 mg/l), and HCO$_3$(136∼146 mg/l). Oxygen and hydrogen isotope ratios of thermal water indicate a meteoric water origin. The activities of Rn-222 and Ra-226 in both thermal water and local groundwater were determined to delineate possible geochemical controls on the Rn-222 and Ra-226. The Rn-222 concentrations are several orders of magnitude greater than the Ra-226 concentrations. The concentrations of Rn-222 range from 190 to 7.490 pCi/1 with an average of 2,522 pCil/l. and those of Ra-226 average 0.32 pCi/1 with the range from 0.25 to 0.42 pCi/1. The concentrations of Rn-222 and Ra-226 are inversely correlated with EC and alkalinity. The pH it positively correlated with Ra-226. The correlation between Rn-222 and Ra-226 is poor. Thermal waters in the study area are produced from highly fractured phyllite. The thermal water qualify. CSAMT (controled-source audiofrequency magnetotelluric) prospecting, and petrological evidences, however, indicate that the heat is possibly transmitted through deep normal faults reaching a deep granite batholith, and the phyllite acts only as a groundwater pathway.

• Korean journal of applied entomology
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• v.43 no.3 s.136
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• pp.195-200
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• 2004

The occurrence pattern and bionomics of Selatosomus puncticollis (Motschulsky) were surveyed in three net house (6$\times$70m) in the field and in a laboratory ($20^{\circ}C$, RH$75\%$, L16/DB). Emergence of hibernated adults of S. puncricollis started from early May, reached peak at early June and diminished in late June. Occurrence rate to total number was $42.1\%$ in early June and $31.7\%$ in late May, when most of hibernated adults emerged at these periods. Adults started to oviposit from mid June, and eggs hatched from early July Larvae turned into pupae from mid July and emerged to adults from mid August. Egg-period was 23 days and pupal period was 21 days. It is estimated that preovipositional period and larval period were approximately 10 months and 30 months, respectively. Wireworms were distributed at the soil depth of 10-5 cm, $56.8\%$ in 1997 and $45.8\%$ in 1998. To establish bait techniques to attract wireworms in the soil, six baits: pieces of potato, carrot, and sweet potato, wheat grains, corns, and flour dough wrapped with gauze, were buried at 15 cm of soil depth, and collected after 5 and 10 days. The numbers of wireworms attracted by potato pieces, wheat grains and sweet potato pieces after 10 days were 1.8, 1.6, and 1.4/bait, respectively. Therefore, burying potato pieces at 15 cm of soil depth and collected after 10 days could be recommended as a wireworm baiting technique.

• Journal of the Korean Society of Radiology
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• v.7 no.6
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• pp.377-381
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• 2013

The study attempted to analyze items presented in Act on safety control of radioactive rays around living environment, which has been recently enacted. The test items have been divided into cosmic rays, cosmic rays, terrestrial radiation, and byproduct, etc., and the selected locations for measurement included an airplane at 8000m in the air, mountainous area at 1000m above sea level, 15m-underground building, construction site, and seashore at 0m altitude. The test showed that, based on cosmic rays, plane at 8000m in the air had 4.91mSv/y of effective dose per year. The mountainous area at 1000m above sea level, which was chosen to measure cosmic rays and terrestrial radiation, was measured 0.35mSv higher than the seashore at 0m in altitude due to the effect of cosmic rays and terrestrial radiation from the greater height above sea level. The construction site, chosen as a location to measure byproduct, showed the highest value among the items with 6.66mSv, which is as 10times high as that of a completed building. The seashore at 0m in altitude had 5.96mSv, and, 15m-underground building, based on terrestrial radiation, was the lowest with 4.91mSv. This suggests that, despite the assumption that terrestrial radiation will have greater effect deeper underground, it did not affect inside the building significantly. This study showed that the items presented in Act on safety control of radioactive rays around living environment were not close to effective dose limit for radiation workers proposed by ICRP. However, they were between 4 and 7 times higher than that for general public. This suggests that there should be continuous research on and attention to Safe Management of Daily Surrounding Radiation Act, which is still at its beginning stage.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.31 no.6
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• pp.468-477
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• 2019

The numerical model of Boussinesq approximation, which is mainly used for evaluating the port calmness due to the irregular waves, has a limit of applicability of lattice size in ports such as marinas with narrow port openings of around 30m. The SWASH model controls the partial reflection according to the depth, porosity coefficient and structure size when applying the reflected wave incident on the structure and terrain. In this study, the partial reflection evaluation at the front of the structure according to the bottom shape and the shape of the structure are examined. In order to evaluate the reproducibility of the model due to the diffraction waves entering the term, the area of incidence at right angles and inclination of the structure is constructed and compared with the diffraction theory suggested by Goda et al. (1978). The experimental results of the sectional structure reflectances calculated as the depth mean show reflectances similar to the approximate values of the reflectances presented by Stelling and Ahrens (1981). It is considered that the reflected wave is well reproduced according to the control of the reflected wave at the boundary and the shape and topography of the structure. Compared with previous studies to examine the diffraction of the wave incident from the breakwater opening, the wave incidence angle and the shape of the diffraction wave are very similar to the theoretical values, but both oblique and rectangular incidence In the case where the direction concentration is small, the diffraction degree is underestimated in some sections with the crest ratio of 0.5 to 0.6.