• 생약학회지
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• 제35권4호통권139호
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• pp.283-287
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• 2004

The effect of water extract from Cnidii Rhizoma (CRW) on proliferation of human breast cancer cells, nitric oxide production, nitric oxide synthase expression, and ornithine decarboxylase activity was tested. CRW inhibited the growth of both estrogen-dependent MCF-7 and estrogen-independent MDA-MB-23I human breast cancer cells. Lipopolysaccharide-induced nitric oxide (NO) production was significantly reduced by CRW at the concentration of 0.5, 1.0 and 5.0 mg/ml. Expression of inducible nitric oxide synthase (iNOS) was also suppressed with the treatment of CRW in Raw 264.7 cells. CRW inhibited induction of ornithine decarboxylase by 12-0-tetradecanoylphorbol-13-acetate, a key enzyme of polyamine biosynthesis, which is enhanced in tumour promotion. Therefore, CRW is worth further investigation with respect to breast cancer chemoprevention or therapy.

• 대한예방한의학회지
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• 제19권2호
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• pp.123-133
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• 2015

The new formular for herbal medicine-safety classification in terms of evidence-based medicine was developed and applied to evaluate various herbal medicines in the previous study. This study is aimed to evaluate the frequently-used herbal medicines inducing toxic metabolites or reactive intermediates(RI), such as Ligusticum wallichii Franch, Angelica sinensis, Glycyrrhizae Radix, Rehmanniae Radix, based on 6 safety grades calculated from human equivalent dose(HED)-based MOS(margin of safety). HED-based MOS can be explained as the ratio of theoretical ALD(approximate lethal dose) of human as $LD_1$(lethal dose of 1%)/ clinical maximum dose as $ED_{99}$(Effective dose of 99%). The herbal medicine showing the ratio less than 1 belongs to Class 1, but the herbal medicine showing the ratio more than 500 belongs to Class 6 with the lowest toxicity. As a result, they were evaluated as class 2 for Angelica sinensis and Glycyrrhizae Radix, class 3 for Ligusticum wallichii Franch and Rehmanniae Radix. These resultant grades for 4 herbal medicines were lower than the grade expected under consideration that these herbal medicines are used very frequently in oriental clinics. These low grades would be due to their ingredients which is biotransformed to toxic metabolites.

• 한국응용곤충학회지
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• 제40권2호
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• pp.149-153
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• 2001

Fungus gnats, Bradysia spp.의 기주를 조사한 결과 오이, 멜론, 수박, 애호박, 토마토, 고추, 파프리카, 백합, 카네이션, 장미, 거베라, 국화, 호접란, 글라디오러스, 생강, 천궁, 작두콩, 신선초, 등굴레, 소나무, 해송 등 21종이 조사되었고 이들 유충은 작물의 지제부나 뿌리근권을 가해하여 뿌리절단, 줄기내 침입 등으로 생육저해, 시들음 증상, 식물체 고사의 원인이 된다.

• 생약학회지
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• 제34권4호통권135호
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• pp.297-302
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• 2003

Cnidii Rhizoma water extract (CRW) was tested for liver cancer chemopreventive potential by measuring the inhibition of phase I enzyme and benzo[a]pyrene-DNA adduct formation and induction of phase II detoxification enzymes. There was 17.0% inhibition in the activity of cytochrome P450 1A1 enzyme with the treatment of 150 mg/ml CRW. At concentration of 30 mg/ml CRW, the binding of $[^3H]B[a]P$ metablites to DNA of NCTC-clone 1469 cell was inhibited by 33.3%. CRW was potent inducer of quinone reductase (QR) and glutathione S-transferase (GST) activities in cultured murine hepatoma Hepalc1c7 cells. However, hepatic glutathione (GSH) level was not influenced by CRW. These findings suggest that CRW has chemopreventive potential of liver cancer by inhibiting cytochrome P450 1A1 activity and benzo[a]pyrene-DNA adduct formation and inducing QR and GST activities.

• KSBB Journal
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• 제22권3호
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• pp.139-145
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• 2007

암에 효능을 보이는 생리활성소재의 탐색원을 발굴하기 위한 방법의 하나로 동양의학 데이터베이스의 처방정보를 활용하였다. 신동의약보감에 포함되어 있는 170건의 암 처방 천연약재의 처방빈도와 처방분량을 분석하였다. 처방순위와 처방 분량에 따라 마련된 점수표 (score table)를 기준으로 총처방점수 (total prescription score)를 작성하였다. 선행연구 결과들을 조사한 결과 총처방점수가 높은 약재들을 소재로 한 관련 보고들이 많은 것으로 나타났다. 결과적으로 당귀, 황기, 감초, 반하, 백출, 진피, 인삼, 백작약, 대황, 천궁, 지모, 천남성, 오미자, 형개, 흑축, 방기, 복령, 오수유 등이 암에 효과가 있으며 이들 약재를 이용한 항암 소재의 탐색이 제안되었다.

• 생약학회지
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• 제27권3호
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• pp.282-287
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• 1996

Cnidii rhizoma is one of the most important crude drugs used particularly for the treatment of female genital inflammatory diseases in traditional oriental medicine. In this study, its anti-inflammatory and analgesic activities were examined employing animal models. It was found that $H_2O$ extract and n-BuOH fraction inhibited significantly the edema formation after the subplantar injection of carrageenin at oral doses of 100 and 200 mg/kg in a dose-dependent fashion, where MeOH extract showed significant anti-inflammatory activity at a oral dose of 200 mg/kg. In Freund's complete adjuvant-induced arthritis, H2O extract and n-BuOH fraction exerted their significant inhibitory activity on the edema formation at oral doses of 100 and 200 mg/kg. The $H_2O$ extract and n-BuOH fraction also showed significant analgesic activity in a dose-dependent manner in acetic acid-induced writhing test.

• 동의생리병리학회지
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• 제19권4호
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• pp.938-944
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• 2005

Down-regulation of melanin synthesis is required for recovery of pigmentary disorders and it is known that direct inhibitors of tyrosinase suppress melanin synthesis. We screened several oriental medicinal plants using B16/Fl0 cells and found that the methanolic extract of Cnidii Rhizoma down-regulated melanin synthesis effectively. Although the proliferation of Bl6/Fl0 cells was decresed by the methanolic extract of Cnidii Rhizoma, it did not appear necrosis. Bl6/F10 cells incubated with the methanolic extract of Cnidii Rhizoma showed reduced pigmentation and tyrosinase activity. Western blotting revealed that the amount of tyrosinase was decreased by the methanolic extract of Cnidii Rhizoma. These results suggest that the inhibitary effect of the methanolic extract of Cnidii Rhizoma on melanogenesis is due to the suppression of tyrosinase in Bl6/F10 cells and Cnidii Rhizoma is a candidate for an efficient whitening agent.

• 한국약용작물학회지
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• 제18권6호
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• pp.373-378
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• 2010

In order to obtain basic data for utilization of the aerial parts of Cnidium officinale Makino (APCO), the antioxidant properties of the aerial parts and rhizomes of C. officinale were measured using DPPH and ABTS radicals, and nitrite scavenging assays. The ethyl acetate (EA) fraction prepared from the aerial parts of APCO showed the strongest antioxidant activities, and contained high level of total phenolic compounds (325.81 mgTE/g) and flavonoids (259.16 mgRE/g). The concentrations for 50% reductions ($RC_{50}$) values of the DPPH and ABTS radicals, and nitrite by the EA fraction of APCO were $11.27\;{\mu}g/m{\ell}$, $14.34\;{\mu}g/m{\ell}$, and $10.26\;{\mu}g/m{\ell}$, respectively. APCO exhibited approximately 3-9 times higher antioxidant activity than rhizomes of C. officinale. The antioxidant capacities of APCO were positively correlated with its total phenolic contents. Therefore, it was concluded that the aerial parts of C. officinale can be a useful and cost-effective source of natural antioxidant for food or cosmetics.

• 한국약용작물학회지
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• 제28권2호
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• pp.111-118
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• 2020

Background: Cnidium officinale is a medicinal crop sensitive to high temperature. It is necessary to develop environment control technology that can reduce environmental stresses such as high temperature. This study was conducted to develop technology for stable production of Cnidium officinale by reducing damage owing to high temperature by applying shading treatment of varying duration, and structure. Methods and Results: Black shading nets were used from May to September or November; shading structures such as pillar, flat roof, and tunnel type structures were installed. Environmental changes, rate of photosynthesis, and growth characteristics were investigated. The shading treatment reduced temperature by 3℃. The rate of photosynthesis and yield with shading treatment were higher by 134% and 127%, respectively, than those with full sunlight. The ratio of shading area ranged from 50% to 71% according to the type of shading structure. The effect of environmental control on growth varied depending on the type of shading structure. Conclusions: The shading treatment reduced damage owing to high temperature, shading rate of 55% - 75% was recorded between the period May - September, and the flat roof type shading structure was considered the most suitable among shading methods.

• 한국지역사회생활과학회지
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• 제26권2호
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• pp.351-360
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• 2015

The study examines the effects of the Cnidium officinale Makino water extract on hyperlipidemia. For this, three levels (low, moderate, and high) of the Cnidium officinale Makino water extract were supplemented to six-week-old male sprague Dawley rats for five weeks. According to the results, serum total lipid, triglyceride, total cholesterol, and LDL cholesterol levels showed significant decreases (p<0.05) in the group with the Cnidium officinale Makino water extract. The serum total lipid level decreased by 8~22%; the triglyceride level, by 13~25%; the total cholesterol level, by 6~23%; and the LDL cholesterol level, by 14~25%. Total cholesterol and triglyceride levels in the liver were significantly lower in the group with the Cnidium officinale Makino water extract than in the control group (p<0.05), and fecal total cholesterol and triglyceride levels were significantly higher in the group with the Cnidium officinalle Makino water extract. These results suggest that the Cnidium officinale Makino water extract in the hypercholesterol diet may reduce hyperlipidemia, a major cause of cardiovascular disease, by reducing serum cholesterol and triglyceride level in the liver through the facilitation of their excretion by feces.