• Title/Summary/Keyword: 채종

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Expression patterns of proliferating cell nuclear antigen in the small intestine of mice infected with Metagonimus yokogawai and Metagonimus Miyata type (요코가와흡충 및 미야타흡충 감염 마우스 소장의 PCNA발현 양상)

  • Yu, Jae-Ran;Myeong, Na-Hye;Chae, Jong-Il
    • Parasites, Hosts and Diseases
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    • v.35 no.4
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    • pp.239-244
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    • 1997
  • Genus Metasonimus has been a subject of taxonomic debates for several years. In morphological aspects, M. vokogawci has been thought to have three subtypes, namely Yokogawa type (M. yokogawai in strict sense) , Miyata type and Koga type. But dif- ferences in the intestinal pathology induced by these subtypes have not been studied yet. In this study we compared the pathological reactions inducted by M. yokoBnwai and Metcgonimus Miyata type using proliferating cell nuclear antigen (PCNA) index. Metacercariae (Mc) of M. yokogcuani were collected by artificial digestion of Plecoslossw oLtiveLis and Mc of Metngonimus Miyata type were collected from Zacco pEntvpus. Three hundreds Mc of each species were infected orally to ICR mice. The mice were sacrificed at 3, 6. 10. 16 and 23 days after infection and the small intestines were resected into three portions (proximal. middle, and distal) . Immunohistochemical staining for PCNA was done using PC-10 (DAKO-PCNA, CA, USA) The PCNA indices in M. Wokosnwci infected group on the 6th and 23rd day after infection were lower than in the control and Miyata type infect ed groups (p < 0.05) from all of the three intestinal regions. On the other hand, the control group and Ifetngonimus Miyata type infected group did not make any differences in PCNA indices. The villus/crypt WIC) ratio was also decreased significantly in M. Wokogcwni infected mice but not in Metagonimus Miyata type infected ones. It is suggested that M. wokogawci induce villous atrophy through a decrease in the cell proliferation at the crypt. The results of this study suggested that M. WokoBnwai induce more serious intestinal pathology than Metcsonimuf Miyata type.

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A Study on Kitchen Knives Used in the Homicide Crimes (최근 살인 사건의 살해도구에 관한 연구 - 칼을 중심으로 -)

  • Jeong, Suk-Joon;Chae, Jong-Min
    • Journal of forensic and investigative science
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    • v.1 no.2
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    • pp.31-43
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    • 2006
  • I searched the recent murder cases for other criminal methods of homicides the monthly statistics of the local police agencies of the whole country, I found that there are many criminal cases where knives were used. Based on this finding, I decided to conduct my survey about kitchen knives. The statistics indicated more than five hundread male and female adults were reported to have committed murders. Of the 500 people, 21 used a kitchen knife to commit murder. This study showed that when people have a conflict, the kitchen knife is as a tool that can be easily changed into a weapon. If the points of the knife is round, more homicides could be avoided. If regulations were established, as we have with dagger when producing, selling or purchasing, I would assume that the number of the crimes with knives will be remarkably diminished. It is our responsibility to create a bill concerning regulatory standards in understanding the dangers of kitchen knives. We must immediately participate in active publicity campaigns.

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The Search of the Crime Prevention Method through the Crime Pattern to Apartment Type (아파트의 형태에 따른 범죄유형과 범죄예방 방법 모색)

  • Choi, Hwan-Young;Chae, Jong-Min
    • Journal of forensic and investigative science
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    • v.2 no.1
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    • pp.23-31
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    • 2007
  • Apartments are built in small countries to accommodate densely populated cities and maximize urbanization. Many apartment complexes have been built in recent reconstruction or redevelopment projects. An increase in crime has resulted due to residents living in a narrow space. Larceny is the most commonly reported crime in apartment complexes. Apartments can be classified as stairway, hallway, or plane surface. This study compares and analyzes the frequency of theft by apartment type to assist in creating a safer residential space. In America and England, scholars studied to make a safer residential space, and have applied the theory of 'the Defensible Space' and 'Crime Prevention Through Environmental Design(CPTED)' since 1970s. Korean apartment design now reflects CPTED in new apartment construction. In this study, 12 apartment complexes were selected in Changwon city to conduct analysis of theft in selected complexes. The study will cover housing invasion theft, motorcycle and car theft and snatching. The most frequency larceny is motorcycle and car theft, the second is housing invasion theft, and the least frequent is snatching. More residents' motorcycles and cars are damaged in a hallway style apartment. More frequently inhabitants have their possessions snatched on a stairway form. 1) When we build new apartment complexes, we must plan to improve territoriality and enhance a natural surveillance by reinforcing dwellers' relationship. Through planning we can prevention the larceny in apartments.

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Crown Shape Control of Pinus koraiensis S et. Z. (III) - Crown Shape Types for Seed and Timber Production from the Trees under Age Class III - (잣나무의 수형(樹型) 조절(調節) (III) - III영급(齡級) 이하(以下) 인공림(人工林)에서 잣과 목재(木材) 생산(生産)을 위한 수형(樹型) -)

  • Yi, Jae-Seon;Song, Jeong-Ho;Park, Moon-Han;Han, Sang-Urk
    • Journal of Korean Society of Forest Science
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    • v.88 no.2
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    • pp.195-204
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    • 1999
  • To improve nut production and timber quality in a plantation, crown shape controls were suggested for Korean white pines (Pinus koraiensis) under age class III through the analyses of the crown shape controls for seed orchard trees in several species, of crown status of controlled and uncontrolled trees in a Korean white pine seed orchard, and of growth characteristics of plantation trees under age class III. For nut production from the small trees less than 8 m, modified leader type can be applied by cutting the central leader and all branches below 1 m and keeping 4~5 nodes with 3~4 whorled branches a node locating 1 m apart. Trees with a 4~8 m long trunk below the first living branch are shaped into trees for nut and timber production, which have a 4~8 m long knotless stem and a modified leader type crown with 4~5 nodes with 3~4 whorled branches a node at a distance of 1 m. Trees, showing the first living branch at or above 9 m from the surface, are pruned severely for timber production and result in a central leader type containing two fifths of the initial crown. It is advisable to remove the leader and the branches when collecting cones or after/during October to take advantage of easy labor and little resin while cutting. After stem cut, branches showing apical dominance are to be cut to control height when necessary. The removal of the uppermost node may be needed to control the crown shape several years after the initial stem-cut.

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Effects of Clones, Ortet Age, Crown Position, and Rooting Substance upon the Rooting of Cuttings of Japanese Larch (Larix leptolepis S. et Z. Gordon) (삽수(揷穗)의 클론, 모수령(母樹齡), 채취부위(採取部位) 및 발근촉진제(發根促進劑)가 낙엽송(落葉松)(Larix leptolepis S. et Z. Gordon)의 삽목발근(揷木發根)에 미치는 영향(影響))

  • Chung, Durk Young;Lee, Kyung Joon
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.205-210
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    • 1994
  • This study was conducted to develop a method of mass production by cutting of superior genotypes of Larix leptolepis and to examine the effects of ortet age, clone, crown position, and an applied plant growth substance upon the rooting of cuttings. Four different ortet ages, 2, 8, 16 and 30 years and three levels of root-promoting substances, indole butyric acid(IBA), at 1000, 2000, and 5000ppm were employed. Greenwood cuttings were taken from mother trees in late July and rooted for three months in rooting medium containing peatmoss : vermiculite : perlite(1 : 1 : 1 by volume). with temperature controlled at $20-25^{\circ}C$, humidity by intermittent misting and light by partial shading in the greenhouse. Cuttings treated with 1000ppm IBA showed highest rooting, with 80%, 71%, 52%, 25% for 2-, 8-, 16-. 30-year-old ortet, respectively. Untreated cuttings showed rooting of 52%, 48%, 36%, 20% for the ortet age of 2, 8, 16, 30 years, respectively. The average number of adventitious roots decreased with increasing ortet age from 2 to 30 years, whereas IBA treatment increased the number of roots in all ages. A variation in rooting among 20 clones tested was observed. The 4 clones, Chungnam 6, and 7 and Chonbuk 1, and 9 showed good rooting of about 67%, while Kangwon 49 showed boor rooting of 40% at 1000ppm IBA treatment. When crown position was compared, cuttings taken from middle crown showed highest rooting of 60%, while cuttings from upper crown showed lowest rooting of 53%.

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Effect of Low-Dose Gamma Radiation to Enhance Germination Rate in Bottle Gourd and Pumpkin Seeds (참박과 호박 종자의 발아촉진을 위한 저선량 감마선의 효과)

  • Kim, Jae-Sung;Baek, Myung-Hwa;Lee, Young-Keun;Lee, Hae-Youn;Yoo, Jun-Cheol
    • Korean Journal of Environmental Agriculture
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    • v.21 no.3
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    • pp.202-207
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    • 2002
  • To establish the seed treatment method of the gamma radiation for germination enhancement of bottle gourd(Lagenaria siceraria Standl.) and pumpkin(Cucurbita ficifolia Bouche), seeds were irradiated with the dose of 1$\sim$20 Gy of the gamma radiation. The germination rate in irradiated group was higher than that of the control, depending on seed condition. In the case of bottle gourd, the germination rate of 70$\sim$80% germinative seed was increased at 4$\sim$20 Gy irradiated groups. Especially, it was the highest at 4 Gy irradiated group. The germination rate of 80$\sim$90% germinative seed was the highest at 4 Gy irradiated group. The germination rate of 70$\sim$80% and 80$\sim$90% germinative seed at 4 days after sowing was increased 66% and 26% at 4 Gy irradiated group, respectively. In the case of pumpkin, the germination rate of 70$\sim$80% and 80$\sim$90% germinative seed was increased at 2 Gy and 8 Gy irradiated group, respectively. Effect of the low-dose gamma radiation on the early growth was enhanced depending on their germination enhancement. Especially, it was the highest at 2 Gy irradiated group of 70$\sim$80% germinative seed of pumpkin. Catalase activity of bottle gourd was increased at 4 Gy irradiated group. Catalase and peroxidase activity of pumpkin were increased at 2 Gy and 8 Gy irradiated group. These results suggest that the germination, early growth and physiological activity of bottle gourd and pumpkin seeds could be promoted by the low dose gamma radiation.

Mucosal mast cell responses to experimental Metagonimus yokogawai infection in rats (요꼬가와흡충 감염에 대한 흰쥐 장 점막 비만세포의 반응)

  • 채종일;김태흥
    • Parasites, Hosts and Diseases
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    • v.31 no.2
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    • pp.129-134
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    • 1993
  • Intestinal mucosal mast cell (MMC) responses were studied in rats experimentally infected with Metngonimur yokogawai (Dlgenea: Heterophyidael. Twenty Sprague-Dawley rats were fed each 2,500 metacercariae isolated from the sweetish and sacrificed on the week 1, 2, 3 and 4 post-Infection (PI). Recovery of worms was performed from the small intestine of each rat. To visualize the MMCs, duodenal and jejunal (upper, middle and lowers) tissue sections were made and stained with alcian blue/safranine-0. The average worm recovery rates were 16.2% and 13.8% on the week 1 and week 2, respectively, but they decreased rapidly to 4.1% and 4.2% on the week 3 and week 4 PI, respectively, which indicate spontaneous worm expulsion after the week 2. The MMC number In the Infected rats was, compared with uninfected controls, significantly Increased In the whole small intestine, through the whole period of observation. The peak level of mastocytosis was observed on the week 3 PI. It is strongly suggested that MMCs might be involved In the expulsion process of flukes from the rat intestine.

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Intestinal mastocytosis and goblet cell hyperplasia in BALB/e and C3H mice infected with Neodiplostomum seoulLense (서울주걱흡충 감염 BALB/c 및 C3H 마우스에서 장점막 비만세포 및 배세포의 증식)

  • 채종일;김태규
    • Parasites, Hosts and Diseases
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    • v.36 no.2
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    • pp.109-120
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    • 1998
  • Mucosal mast cell (MMC) and goblet cell (GC) responses were observed in the small intestine of two strains of mice (BALBfc and C3H) infected with Weodiplostomum seouLehTe, and their roles in the host defense and worm expulsion were studied. From day 3 to 28 post-infection (Pl) with 200 metacercariae, the worm recovery rate from BALB/c mice was consistently and remarkably higher than that from C3H mice. In the duodenum of both strains of mice, the main habitat of the flukes, mastocytosis was pronounced on day 7 Pl but quickly diminished thereafter. Similar kinetics were observed in the jejunum and ileum, although the extent of mastocytosis was lesser in the ileum than other two areas. These MMC kinetics were not different between the ko strains of mice. Moreover, the extent of mastocytosis was stronger in BALB/C mice than in C3H mice. GC hyperplasia was remarkable in the duodenum of BALBlc mice throughout the course of infection except day 14 Pl, whereas it was recognizable only in the jejunum and ileum of C3H mice on day 7 Pl. Mucin activation was evidently demonstrated in both strains of mice throughout the course of infection, but more marked in BALB/c than in C3H mice. The results strongly suggest that mastocytosis and GC hyperplasia are local immune responses against N. seoulense, however, they play a minor role in the host defense and worm expulsion.

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Biological Control of Phytophthora Blight and Anthracnose Disease in Red-pepper Using Bacillus subtilis S54 (Bacillus subtilis S54 균주를 이용한 고추 역병과 탄저병의 생물학적 방제)

  • Lee, Gun-Woong;Kim, Myung-Jun;Park, Jun-Sik;Chae, Jong-Chan;Soh, Byoung-Yul;Ju, Jae-Eun;Lee, Kui-Jae
    • Research in Plant Disease
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    • v.17 no.1
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    • pp.86-89
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    • 2011
  • Phytophthora blight and anthracnose disease caused by Phytophthora capsici and Collectotrichum gloeosporioides are the most important devastating diseases of red pepper plants, worldwide. Five different bacterial isolates were isolated from the red pepper rhizosphere and non-rhizosphere soil and subsequently tested for antagonistic activity against P. capsisi and C. gloeosporioides. The area of the inhibition zone was taken as a measure for antagonistic activity. Among the 5 isolates tested, S54 exhibited a maximum antagonistic activity under in vitro and in vivo conditions. In greenhouse studies the isolate has successfully reduced the disease symptom. Protect value was 80.8% (Phytophthora blight) and 81.9% (Anthrancnose disease), whereas the infection rate of control plants was 21.3% and 23.2%. Based on the 16S rDNA sequence and API 50CHB Kit analysis the most effective isolate was identified as Bacillus subtilis. The results of the study indicate that the stratin S54 could be used as an potential biological control of Phytophthora blight and anthracnose disease of red pepper.

Growth Characteristics and Productivity of Single Cross Maize Hybrid for Grain, 'Andaok' (다수성 종실 옥수수 신품종 '안다옥'의 생육특성과 수량성)

  • Son, Beom Young;Baek, Seong Bum;Kim, Jung Tae;Lee, Jin Seok;Ku, Ja Hwan;Kwon, Young Up;Huh, Chang Suk;Park, Jong Yeol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.1
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    • pp.1-5
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    • 2013
  • Andaok, a new single cross variety, is a yellow dent maize hybrid (Zea mays L.) developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2011. This hybrid, which has a high yield of grain, was produced by crossing two inbred lines, KS161 and KS162. KS161 is the seed parent and KS162 is the pollen parent of Andaok. Silking date of Andaok is 3 days later than that of the check hybrid, Jangdaok. Plant height of Andaok is longer than that of Jangdaok. Ear numbers per 100 plants of Andaok is more than that of Jangdaok. Ear length of Andaok is shorter than that of Jangdaok. The weight of 100 seeds of Andaok is heavier than that of Jangdaok. It has moderate resistance to southern corn leaf blight (Bipolaris maydis), black streaked dwarf virus (BSDV) and corn borer. It has strong resistance to northern corn leaf blight (Exserohilum turcicum). It has resistance to lodging. Andaok was evaluated for its yield of grain at three locations from 2009 to 2011. The grain yield of Andaok was 7.80 ton/ha. Seed production of Andaok has gone well due to a good match during crossing between the seed parent, KS161, and the pollen parent, KS162, in Yeongwol.