• Archives of Plastic Surgery
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• v.35 no.4
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• pp.355-359
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• 2008

Purpose: Oncostatin M(OSM) has been known as a role in fibrosis and anti-inflammatory effects of various organs and tissues. Although there have been a number of studies which are focused on the roles and mechanisms of OSM, there are few reports on its effects in chronic wound healing. The purpose of this study is to evaluate the effects of OSM in wound healing activities of dermal fibroblasts of chronic wound in vitro. In particular, this study is focused on cell proliferation and synthesis of collagen and glycosaminoglycan(GAG), which are the major components of the extracellular matrices, of diabetic fibroblasts. Methods: Fibroblasts were isolated from excess skin that was obtained from diabetic foot ulcer patients who underwent debridement. The isolated fibroblasts were cultivated in presence of OSM(100 ng/mL). Cell proliferation, collagen synthesis and GAG levels were compared. Results: All the components tested in this study increased in OSM treatment group. In particular, collagen and GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). Conclusion: These results indicate that OSM increases wound healing activities of dermal fibroblasts of chronic wound in vitro.

• Archives of Plastic Surgery
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• v.35 no.4
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• pp.400-406
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• 2008

Purpose: Since ancient times, larval therapy has been applied to help wound healing. Its use has recently been rediscovered, and the interest in this therapy has been increased in clinical practice, as well as in research. The objective of this study is to assess the efficacy of maggot therapy for the treatment of intractable, chronic wounds. Methods: Twenty patients, suffering mostly from chronic wounds were treated using maggots of greenfly (Phaenicia sericata). Sterile maggots were administered to the wound twice a week. The causes of the development of wounds were diabetic foot(14), trauma(3), plate exposure(1), and unknown origin(2). Results: Complete debridement was achieved in 10 wounds; in 8 wounds, the debridement was partially achieved; 2 patients escaped. Four patients were healed without any additional surgical procedures but fourteen patients were treated with skin graft. Pain, reported by six patients, was relatively well controlled with oral analgesics. Conclusion: We found larval therapy to be effective for chronic wounds. Maggot therapy is a relatively rapid and effective treatment, particularly in necrotic wounds or the wounds resistant to conventional treatment.

• The Journal of Korean Physical Therapy
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• v.16 no.3
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• pp.50-64
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• 2004

The studies have been designed to evaluate the effect of wound healing of pulsed ultrasound and chitosan application in diabetic incisive wound of rats. Mild diabetes mellitus was induced in rat used 30 mg/kg streptozotocin. Full thickness skin incision was made on the backs of Sprague-Dawley rats. We used 72 Sprague-Dawley rats which were divided into 4 groups; the subjects were divided into group of 6 rats each 3, 6, and 15 days. The results were summarized as follows; The rate of wound length of pulsed ultrasound with chitosan application groups more decreased than only pulsed ultrasound treatment group. The density of inflammatory cells in the experimental groups was more significantly decreased than diabetic control group(p<0.05). Historically, in the ultrasound with chitosan application groups, reepithelized epithelium was thicker and the collagen fiber were organized in a liner manner and connective tissue was matured faster those of the diabetic control group(p<0.05). From the conclusions above, in this study application of pulsed ultrasound and chitosan can be an effective way of promotion of wound healing in diabetic model.

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.382-385
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• 2010

This study was performed to investigate the wound healing effects of Allium cepa L. on Staphylococcus intermedius contaminated wound. 4 full-thickness skin wounds ($2\;cm{\times}2\;cm$) were made in the back and Staphylococcus intermedius was inoculated in seven dogs. In each dog, the wounds were treated with onion extract (experimental group) and normal saline (control group), respectively. The wound contraction rate of the experimental group was higher than that of the control group during the experimental period. The occupation rate showed that the wounds of the experimental groups contracted more vigorously than that of the control group in the early period of wound healing. The mean value of tensile strength on the 22 day after wounding was $4,741{\pm}848\;g/cm^2$ in the experimental group and $2,400{\pm}397\;g/cm^2$ in the control group. The number of S. intermedius in the experimental groups was decreased more rapidly than that of the control group. These results suggest that application of activated onion extract in like full-thickness skin wounds promote wound healing.

• Journal of Korean Physical Therapy Science
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• v.10 no.1
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• pp.198-205
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• 2003

We evaluated the effect of low power GaAsAl laser on re-epithelization in full-thickness excisional wound of rat skin. Two full-thickness excisions were made on the back of the experimental animals. Low power laser applications with 10mW intensity were treated experimental animals twice a day for 7 days. On the seventh postoperative day the quantitative analysis of re-epithelization was performed using immunohistochemical staining for proliferating cell nuclear antigen (PCNA). The majority of PCNA immunoreactive cells was observed at epithelial cells in the margin of full thickness excisional wound. The low power laser treatments significantly increased the number of PCNA immunoreactive cell as compared to that of non treated animal group (p<0.01). The shape of PCNA immunoreactive cell appeared as small dark, round to ovoid structures. Most PCNA immunoreactive cells exhibited a high intensity of staining that contrasted sharply with the surrounding background. In conclusion, these findings suggest that GaAlAs laser treatments effectively enhance the epithelial wound healing by the stimulating cell proliferation. Furthermore, the majority of cell proliferation occurred in the margin of full thickness excisional wound.

• KSBB Journal
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• v.29 no.1
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• pp.42-49
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• 2014

The aim of this study was to develop a composite human skin equivalent for wound healing. Collagen type1 and acellular dermal matrix powder were utilized as the scaffold with dermal fibroblasts and keratinocytes for the development of a composite human skin equivalent. Fibroblast maintained the volume of composite skin equivalent and also induced keratinocytes to attach and proliferate on the surface of composite skin equivalent. The composite human skin equivalent had a structure and curvature similar to those of real skin. Balb-C nu/nu mice were used for the evaluation of full-thickness wound healing effect of the composite human skin equivalent. Graft of composite skin equivalent on full-thickness wound promoted re-epithelialization and granulation tissue formation at 9 days. Given the average wound-healing time (14 days), the wound in the developed composite skin equivalent healed quickly. The overall results indicated that this three-dimensional composite human skin equivalent can be used to effectively enhance wound healing.

• Journal of Veterinary Clinics
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• v.14 no.2
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• pp.238-243
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• 1997

체중이 700-750kg 인 4마리의 중종마에 70분동안 마취를 실시하여 동맥혈의 이 산화탄소 분압과 호기말 가스내의 이산화탄소 분압 사이의 관계를 관찰하였다. 마취도중 자발 호흡, 인공호흡 1(흡기시간 2.0초), 인공호흡 2(흡기시간 2.5초)를 각각 30분,20분,20분씩 실시하였으며 매 10분마다 동맥혈가스분석과 호기말 가스내 이산화탄소분압 측정을 실시하 였다. 동시에 혈압, 심전도, 체온측정을 실시하여 마취된 환축의 상태를 관찰하였다. 2주후에 자세를 달리하여(앙와에서 측와로) 같은 방법으로 재 실험하였다. 호기말 이산화탄소분압은 동맥혈에서보다 평균 10 mmHg 정도 낮은 양상을 보였으나 높은 상관관계를 보였으며 자세에 따른 유의적 차이는 업었다(앙와자세; r=0.949, 측와자세; r=0.920, P<0.01). 이러한 결과를 토 대로 조직에 창상을 줄 수 있는 동맥혈 가스분석 대신 비침습적방법인 호기말 가스내 이산화 탄소 분압을 측정하는 것이 환축의 모니터링에 효과적으로 사용 가능하다는 것을 알 수 있었다.

• Polymer(Korea)
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• v.38 no.3
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• pp.338-350
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• 2014

Cellulose acetate (CA) fiber mats containing inclusion complexes of asiaticoside (AC) in 2-hydroxypropyl-${\beta}$-cyclodextrin ($HP{\beta}CD$) for potential usage as wound dressings were developed. The AC/$HP{\beta}CD$ complex-loaded CA fibers at various $HP{\beta}CD$ to AC molar ratios of 0.5, 1, and 2 were prepared in 90:10 v/v mixture of 80% (v/v) acetic acid and N,N-dimethylacetamide (DMAc) via electrospinning. The maximum released amounts of AC depended on the $HP{\beta}CD$ content and were much greater than those released from the AC-loaded CA fiber mat. In the in vitro study, indirect cytotoxic evaluation with human dermal fibroblasts (HDFa) showed that these materials released no substances in the levels that were harmful to the cells and the cells appeared to attach and proliferate well on these substrates. However, only the CA fiber mats containing AC/$HP{\beta}CD$ complexes at the $HP{\beta}CD$ to AC molar ratio of 0.5 was effective in upregulating the production of collagen of the cultured cells.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.2
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• pp.225-231
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• 2009

In this paper, it is shown that Carson's equation can still be applied for the calculation of the series reactance of transmission lines with no ground return current as well as the one with ground return. It is proved in the following method. First two voltage drop equations for three-phase three wire transmission line are derived, one without considering ground return and the other using Carson's equation. The impedance matrix of the two equations are different from each other. But if we put the condition of zero ground current, $I_a+I_b+I_c=0$, those two equations becomes the identical equations. Therefore even a transmission line is not grounded, its line parameters can still be obtained using the Carson's equation. It has been confused whether or not Carson's equation can be used for an ungrounded system. It is because where ever Carson's equation is shown in the book, it also says that the system has ground return current paths as a premise. It is also verified with EMTP studies on the test circuit.

• Proceedings of the KIEE Conference
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• 1998.07c
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• pp.908-910
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• 1998

Modern AC electric car has PWM(Pulse Width Modulation) -controlled converters, which give rise to higher harmonics. The current harmonics injected from AC electric car is propagated through power feeding circuit. As the feeding circuit is a distributed constant circuit composed of RLC, the capacitance of the feeding circuit and the inductance on the side of power system cause a parallel resonance and a magnification of current harmonics at a specific frequency. The magnified current harmonics usually brings about various problems. That is, the current harmonics makes interference in the adjacent lines of communications and the railway signalling system. Furthermore, in case it flows on the side of power system, not only overheating and vibration at the power capacitors but also wrong operation at the protective devices can occur. Therefore, the exact assessment of the harmonic current flow must be undertaken at design and planning stage for the electric traction systems. From these point of view, this study presents an approach to model and to analyse traction power feeding system focused on the amplification of harmonic current. The proposed algorithm is applied to a standard AT(Auto-transformer)-fed test system in which electric car with PWM-controlled converters is running.