• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.4
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• pp.1-15
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• 2018

Objectives: Osteoporosis is considered a serious human disease. We developed an extract of mixed herbs containing root of Platycodon grandiflorum (ExMH-PGR), which is expected to be effective in preventing or treating osteoporosis. The aim of this study was to investigate the anti-osteoporotic effect of ExMH-PGR in osteoblastic MC3T3-E1 cells and osteoclastic RAW 264.7 cells. Methods: To examine the anti-osteoporotic effect of ExMH-PGR, osteoblast and osteoclast differentiation were induced and cultured with various concentrations of ExMH-PGR. Alkaline phosphatase (ALP) activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells were analyzed. Tartrate-resistant acid phosphatase (TRAP) activity and the formation of actin ring in RAW 264.7 cells were analyzed. Results: ExMH-PGR at concentration up to $25{\mu}g/mL$ significantly increased ALP activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells. ExMH-PGR at 50 to $200{\mu}g/mL$ significantly inhibited TRAP activity and the formation of actin ring in RAW 264.7 cells. Conclusions: These results demonstrate that ExMH-PGR stimulates osteoblastic activities and inhibits osteoclastic activities in in vitro systems, suggesting that ExMH-PGR might be considered as an anti-osteoporotic candidate for treatment of osteoporosis disease.

• Clean Technology
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• v.24 no.3
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• pp.183-189
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• 2018

Hollow carbon spheres (HCS) and carbon spheres (CS) were prepared by a hydrothermal reaction and they were introduced as a substrate for the deposition of $MnO_2$ nanoparticles. The $MnO_2$ nanoparticles were deposited on the carbon surface by a chemical redox deposition method. After deposition, the $MnO_2$ nanoparticles were uniformally distributed on the carbon surface in a slit-shape, and sparse $MnO_2$ slits appeared on the HCS surface. The $MnO_2-HCS$ showed an initial specific capacitance of $164.1F\;g^{-1}$ at scan rate of $20mv\;s^{-1}$, and after 1,000 cycles, the specific capacitance was maintained to $141.3F\;g^{-1}$. The capacity retention of $MnO_2-HCS$ and $MnO_2-CS$ were calculated to 86% and 78% in the cycle performance test up to 1,000 cycles, respectively. $MnO_2-HCS$ showed a good cycle stability due to the mesoporous hollow structure which can cause a faster diffusion of the electrolyte and can easily adsorb and desorb $Na^+$ ions on the surface of the electrode.

• Food Industry
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• s.181
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• pp.38-67
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• 2004

예로부터 인간은 단맛에 강한 욕구를 가지고 있었다. 따라서 설탕, 포도당, 과당 등 단맛이 강한 감미료를 선호하여 왔고, 현재까지도 식품 제조시 가장 널리 사용되고 있다. 그러나 최근에는 국민의 건강에 대한 관심이 고조 되고 설탕으로 대표되는 전통적인 일반 감미료의 충치, 비만, 당뇨병 유발 등의 부정적인 측면이 부각되고 있을 뿐만 아니라, 식품에 대한 건강 기능성이 강조되면서 기능성 감미료가 주목 받고 있다. 특히 고도의 경제성장 및 국민 소득의 향상에 따라 차별화된 기능성 감미료의 생산을 요구하는 실정이다. 이와 관련하여, 현재 식품 산업계에서는 올리고당류, 당알콜류, 고감미 감미료류 등에 많은 관심을 가지고 연구 개발에 집중하고 있다. 올리고당류의 대표적인 제품은 프락토올리고당, 갈락토올리고당, 이소말토올리고당 등이다. 현재 올리고단 시장은 성숙기로서 성장속도가 둔화되고 있으나, 최근 American Association of Cereal Chemists(AACC)등 세계적인 협회에서 수용성 식이섬유를 다당류, 올리고당, 리그닌 등을 포함하여 새롭게 정의하였고 이에 따라 해외에서는 프락토올리고당을 중심으로 수용성 식이섬유로 용도 사용이 가능하게 되었을 뿐만 아니라, 식이섬유로 표기도 가능하게 되어 수요가 증가할 것으로 예상된다. 국내에서는 아직 올리고당을 식이섬유로 사용하지는 못하고 있으나, 식품 공전 개정을 통해 식이섬유로 표기 가능하도록 수정될 전망이다. 당알콜류는 솔비톨 및 자일리톨이 주류를 이루고 있다. 솔비톨은 시장에 가장 먼저 소개된 당알콜류인데 저가여서 그동안 가장 큰 시장을 형성하고 있었으나 최근 3년 동안 자일리톨껌이 최대의 히트 상품이 되면서 자일리톨이 급성장하였다. 그 외에는 만니톨, 에리스리톨, 말티톨, 이소말트 등이 있다. 최근에는 말티톨을 중심으로 다양한 응용 연구 및 이소말트 및 에리스리톨 등을 생산 판매하고 있는 다국적 기업의 판촉 활동 등으로 당알콜류 시장의 새로운 변화가 예상된다. 고감미 감미료류는 스테비오사이드와 아스파탐이 주류를 이루고 있었지만, 최근 아세설팜 K 및 수크랄로스가 잇따라 식품으로써 사용 허가를 받으면서 시장이 재편되고 있다. 하지만, 해외에 비하여 국내의 고감미 감미료 시장은 매우 작고 성장률도 낮은 편인데 그 이유로는 국민 정서상 천연이 아닌 합성에 대한 부정적 인식과 맛에 대한 거부감 때문인 것으로 생각된다. 이와 관련하여 최근에는 다양한 감미질 및 Multi sweetener system 연구를 통해 설탕과 유사한 맛을 내는 혼합 기술 개발에 관한 연구가 진행되고 있다. 따라서 향후에는 해외의 선진국과 같이 고감미 감미료 시장도 점차적으로 확대될 것으로 예상된다. 기타 대체 감미료로는 타가토스, 트레할로스, 아라비노스 등이 있다. 이중에서 특히 해외에서 크게 기대를 모으고 있는 타가토스는 최근 국내 인증을 받아 시장 진출을 준비 중에 있다. 아라비노스는 양질의 감미를 갖는 천연 펜토오스인데 연구용 시약 이외에는 지금까지 거의 이용되지 않았다. 그러나 최근 아라비노스가 설탕의 소화흡수를 선택적으로 억제한다는 흥미 있는 결과가 임상 및 동물실험에서 확인되어 향후 제품으로써 개발을 기대할 수 있게 되었다. 이들 감미료는 기존 설탕이 가지는 문제점을 많은 부분 극복할 것으로 여겨지며, 이로 인해 수요는 점차 증가할 것으로 생각되며, 높은 경제적 부가 가치를 창출할 것으로 예상되어 진다. 생산 기술 적인 측면에서 건강 지향적 천연소재 감미료에 대한 선호가 날로 증가하는 추세에 따라 기존에 주로 사용되었던 화학합성법보다는 천연 이미지의 생물공학적인 방법에 의한 감미료 생산이 각광을 받을 것으로 예상된다. 생물공학적인 방법을 감미료 소재 개발에 응용하게 되면, 기존에 사용되었던 화학합성법 등이 가지는 환경적인 문제, 반응 부산물 생성에 따른 분리, 정제에서의 비용증가 등의 문제점을 해결할 수 있을 것으로 여겨지며, 고정화 방법의 도입 등을 통해 생산성 향상을 도모할 수 있다. 본 장에서는 감미료 소재 개발에 건강 지향적 국민 정서를 반영하고, 국내외 감미료 시장의 흐름을 파악하여 당류 산업의 발전적인 변화에 대처하고자 하였으며, 이를 통해 당류산업의 고부가가치 첨단산업으로서의 육성방안을 논의하고자 하였다.

• The Journal of the Petrological Society of Korea
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• v.20 no.2
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• pp.99-114
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• 2011

Precambrian gneiss complex in the Pyeongchang-Wonju area, which lies west of the Paleozoic sedimentary basin of the Yeongwol-Taebaek area, is being considered as a part of the Gyeonggi massif, but its ages of formation and metamorphic events are not well defined yet. In this study, SHRIMP zircon U-Pb ages were determined from the gneiss complex in the area, We obtained the discrete ages of magmatic (ca. 1960 Ma) and metamorphic (ca. 1860 Ma) events through the interpretation of the SHRIMP data based on the internal structures of zircons. These are almost the same to the ages of main intrusion and metamorphism reported from the Precambrian basements of Gyeonggi, Yeongnam and Nangnim massifs of the Korean Peninsula, Ages of 3200~3300 Ma, 2900 Ma, 2660 Ma, 2430 Ma, 2260 Ma, and 2080~2070 Ma obtained from inherited cores of studied zircons are also very similar to the frequently reported ages from the basement rocks of the Gyeonggi and Yeongnam massifs, Lower intercept age of about 270 Ma calculated from the rim data seems to indicate that the study area suffered from a late Paleozoic metamorphism (Okcheon Orogeny), but we need more reasonable and sufficient data to confirm it. According to the results of this study, it is suggested that the Bangnim group unconformably overlying the gneiss complex was deposited after the Paleoproterozoic granitic magmatism (ca. 1960 Ma) and metamorphism (ca. 1860 Ma).

• Food Science and Preservation
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• v.22 no.6
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• pp.908-914
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• 2015

The objectives of this study were to isolate and characterize low temperature adaptation yeast and to obtain suitable yeasts strains for manufacturing Yakju. In this study, we isolated 482 wild yeasts from fermented foods. Out of these, 5 yeast strains were selected based on increased growth at low temperature ($15^{\circ}C$) and high ${\beta}$-glucosidase activity. To screen the aromatic level of isolates, media containing cerulenin and 5,5,5-trifluor-DL-leucine (TFL) were used. Y297 strain demonstrated tolerance against TFL and produced more than 13% alcohol. Y297 strain was identified a Saccharomyces cerevisiae based on the 26S rDNA gene sequences. Maximum cell growth was observed after 19 hr and 38 hr of incubation at $25^{\circ}C$ and $15^{\circ}C$, respectively. The exponential phase was followed by a lengthy stationary phase, at $15^{\circ}C$, when the cells remained high viable. Y297 strain demonstrated tolerance against alcohol (10%), glucose (60%) and salt(NaCl, 8%). ${\beta}$-glucosidase and esterase activity in Y297 were higher than those of controls at $15^{\circ}C$. Overall, these results indicated that using wild yeast strain, isolated from fermented food, affects the chemical characteristics of the brewed Yakju.

• Journal of Korean Society on Water Environment
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• v.24 no.1
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• pp.123-128
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• 2008

The Dissolved Organic Carbon (DOC) existing in a water includes both hydrophobic and hydrophilic substances however, most of the discussion focuses on hydrophobic substances. The hydrophobic fraction was easily removed by absorption or coagulation more than hydrophilic fraction. Therefore, control of the hydrophilic fraction is very important in water treatment process. This study is to determine the variation of DOC, the removal efficiency of DOC, and Trihalomethane formation potential (THMFP) after each stage of water treatment process by fractionating Natural Organic Matters (NOM) into hydrophobic and hydrophilic substance. DOC from raw water was fractionated at acidic pH (pH<2) using XAD 8 resin column, into two fraction : hydrophobic substance (i.e. humic substance) adsorbed on XAD 8 and hydrophilic substance which represent the organics contained in the final effluent. THMFP was carried out according to the following set condition: Cl2/DOC=4 mg/mg, incubation at $25^{\circ}C$ in darkness, pH 7 adjust with HCl or NaOH as necessary, and 72hour-contact time. THMs analyzed in this study were chloroform, bromodichloromethane, dibromochloromethan, and bromoform. Sewage was almost evenly split between the hydrophobic (56%) and hydrophilic fraction (44%). But, Aldrich humic substance (AHS) was found to contain less hydrophilics (14%) than hydrophobics (86%). The formation of THMs may depend on the source which is characterized by the composition of organic matters such as AHS and sewage. The THMFP yield of sewage and AHS were assessed as follows. The value of the THMFP reaction yield, AHS $172.65{\mu}g/mg$, is much higher than that of sewage $41.68{\mu}g/mg$. This illustrates possible significant difference in THMFP according to the component type and the proportion of organic matter existing in water source. Apparently AHS react with chlorine to produce more THMFP than do the smaller molecules found in sewage. Water treatment process may reduce THMFP, nevertheless residual DOC (the more hydrophilic substance) has significant THMFP. Further reduction in organic halide precursors requires application of alternative treatment techniques.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.896-904
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• 2007

Regulation mechanism of apoptosis has been known to be important for understanding the pathogenesis of a number of human diseases including cancers. The effects of $RRR-{\alpha}-tocopheryl$ succinate(vitamin E succinate, VES) on the cell viability, generation of ROS, expression of proteins involved in apoptosis, and growth of human chronic myelogenous leukemia K562 cells were analyzed in this study. VES treatment not only induced the generation of the ROS but also increased the levels of $NF-{\kappa}B$, COX-2, and $p21^{WAF1/CIP1}$ in K562 cells. It modulates the levels of pro-apoptotic proteins such as Bax provoking the apoptosis in K562 cells. The cleavage of PARP into 89 kDa was also increased upon VES treatment in a dosage-dependent manner. Induction of an apoptosis was evident by the increase of sub-Gl peak and cell shrinkage condensed chromatin in K562 cells treated with VES. It also resulted in an inhibition of tumor growth by 50% and prolonged survival of the Iymphoma-induced mice. This potentiation of VES obtained in vitro and in vivo may indicate the feasibility of more effective chemotherapy in chronic myelogenous leukemia.

• Journal of Life Science
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• v.23 no.3
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• pp.462-469
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• 2013

Withaferin A is a steroidal lactone purified from the Indian medicinal plant Withania somnifera. It exhibits a wide variety of activities, including anti-tumor, anti-inflammation, and immunomodulation properties. In this review, we focused on the anti-cancer effects of withaferin A. Withaferin A inhibits cell proliferation, metastasis, invasion, and angiogenesis in cancer cells. Furthermore, it sensitized irradiation, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-, and doxorubicin-mediated apoptosis. The results showed that multiple mechanisms were involved in withaferin A-mediated anti-cancer effects. First, withaferin A increased intracellular reactive oxygen species (ROS) production and induced ER stress- and mitochondria-mediated apoptosis. Second, withaferin A inhibited the signaling pathways (Jak/STAT, Akt, Notch, and c-Met), which are important in cell survival, proliferation, and metastasis. Third, it induced apoptosis and inhibited cancer cell migration through the up-regulation of prostate apoptosis protein-4 (Par-4). Finally, withaferin A up-regulated pro-apoptotic protein expression levels through the inhibition of proteasome activity. Our findings suggested that withaferin A is a potential, potent therapeutic agent.

• Korean Journal of Food Science and Technology
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• v.28 no.6
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• pp.1089-1094
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• 1996

The in vivo effect of tyrosinase inhibitors in the melanogenesis of gold fish (jet black color) was evaluated by measuring surface color and observing melanin pigment. The fish was firstly cultivated in 0.9% NaCl solution for 1 week to induce melanogenesis, and then, it was transferred to each treatment group containing tyrosinase inhibitor. The fish was grouped into control. food additive group (addition of 5 mM glutathione, 5 mM cysteine, and 1 mM benzoic acid), microbial inhibitor group (addition of culture broth of Aspergillus oryzae in shiitake and glucose medium), and plant extract group (addition of the mixed extracts of green tea, beet, red chicory, and nameko). After 6 days, the fish was anesthetized by electric shock, and color of pectoral region, lateral region, and dorsal fin was measured. Hunter's L and b values of treated group were generally higher than those of control group, indicating that the tyrosinase inhibitors could inhibit the melanogenesis of the fish. Effect of plant extract was apparent, though relatively weak, not because it did not work in vivo, but because a sufficient amount of extract could not be added to fish globes. If a large amount of extract was added, fish gradually died due to a microbial contamination. Microscopic observation of melanin in lateral scale and dorsal fin showed that in the treated groups with tyrosinase inhibitors, the number of melanophore per unit area and the size of one melanophore decreased.

• Journal of Korean Ophthalmic Optics Society
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• v.5 no.2
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• pp.167-173
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• 2000

Four glasses of $B_2O_3-Bi_2O_3-PbO-SiO_2$ (BBPS) system were prepared by melting the appropriate amounts of reagent grade oxides of $B_2O_3$, $Bi_2O_3$, PbO, and $SiO_2$ in an open crucible. The differential thermal analysis showed crystallization temperature decreased with increasing $Bi_2O_3$ or PbO content in the sample. The structures of glasses system were studied using scanning electron microscopy and Fourier transform-Infra red (FT-IR) spectroscopy. The UV cut-off and refractive index were found to be sensitive to the $Pb^{+2}$ and $Bi^{+3}$ content in the glasses. The behavior of the IR spectra of the glasses in the BP series was consistent with a role of $Bi_2O_3$ as a network former. In the BP series of glasses, the result of IR spectrum indicated that PbO behaved as a network former.