• Title/Summary/Keyword: 질소원

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토양경작법(Landfarming)을 이용한 유류오염토양의 복원효율 평가

  • Jeon Gwon-Ho;Han Wan-Su;Jeong Hyeon-Seok;Seo Chang-Il;Park Jeong-Gu
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2005.04a
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    • pp.219-222
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    • 2005
  • 유류로 오염된 토양에 대해 토양경작법(Landfarming)을 적용하여 복원효율을 평가하였다. 복원 초기에는 주로 휘발에 의한 오염물질의 저감이 이루어졌으며, 이 후 미생물성장에 필요한 토양뒤집기와 질소원 영양물질의 공급으로 인해 미생물수가 복원초기에 비해 약 200배 증가하면서 약 30여일 경과 후 초기 20,000ppm에서 복원목표인 TPH의 토양오염우려기준 2,000ppm의 이하인 1,910ppm으로 감소하였다. 시간이 경과할수록 탄소원 섭취기질 부족으로 완만한 오염물질 감소속도를 나타내면서 최종적으로 TPH를 측정한 결과 1,288ppm으로서 제거효율 94%이상을 나타내었다.

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Sulfotransferase 생산 균주인 K-12의 분리와 배양

  • 김동현;김병택;김은하
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1993.04a
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    • pp.142-142
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    • 1993
  • 생쥐에서 sulfotransferase 생산 군주를 검색한 결과 호기성균보다 혐기성균이 많은 것으로 나타났다. 이러한 것은 전번 보고와 일치한다. 황산전이효소 생산 균주 검색용 배지에서 형광을 나타내는 호기성 균주를 분리하여 K-12라 명하였다. 동정한 결과 K-12균주는 그람 음성으로 운동성이 없는 통성혐기성 균주인 Haemophilus sp.로 사료된다. 이 균주의 황산전이효소 생산성은 공여체 기질만을 배지에 첨가했을때 가장 높은 생산성을 보였다. 탄소원으로는 sucrose와 lactose를 사용했을때 가장 높은 효소유도를 보였으며, 질소원으로는 yeast extract, peptone이 우수하였다. 배양최적 pH는 7부근이었으며 온도는 37$^{\circ}C$가 가장 좋았다. 금속이온으로는 $Mg^{2+}$이 우수했다. 최적 배지 10 L에 K-12 균주를 배양하여, 집균 초음파 처리, 원심분리한 상등액을 70% ammonium sulfate fractionation, DEAE-cellulose column chromatography 2회, Sephcryl S-300 superfine column chromatography를 행한 결과 specific activity가 0.267 $\mu$mole/min/mg protein인 효소를 부분 정제하였다.

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Purification of Extracellular Enzyme Produced by Vibrio sp. AL-145 (Vibrio sp. AL-145가 생산하는 균체외 효소의 정제 (I))

  • 주동식;이응호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.2
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    • pp.234-239
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    • 1993
  • The alginate degrading bacteria have been screened from the marine environment. Sodium alginate and NaCl were required for cell growth and enzyme production of 145-C strain and the adequate concentrations were 0.7 and 2.5%, respectively. The effective nitrogen source was peptone and adequate temperature was 28$\pm$2$^{\circ}C$. The 145-C strain was identified as Vibrio sp. from biochemical and biological experiment. The extracellular enzyme produced by Vibrio sp. was purified and the molecular weight was estimated to be 27, 000.

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Nutritional Effect of Carbonized Tobacco Leaf Debris Neutralized by Nitric and Phosphoric Acid on Nursery Seedlings (연초가공부산물(煙草加工副産物)의 탄화정도(炭化程度)에 따른 질산중화능력(窒酸中和能力)과 비효(肥效))

  • Lee, Yun-Hwan;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.1
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    • pp.89-93
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    • 1985
  • In order to develop the nutrient material for growing plants, tobacco leaf debris (T.L.D) that is a residual product of cigarette processing was carbonated at about 50% of its weight and neutralized its alkalinity by nitric and phosphoric acid. This material ($T.L.D+3N-HNO_3+1N-H_3PO_4$ (10:1:1), as a consequence, contained 4.1% of nitrogen, 2.1% of phosphte, and 6.6% of potassium in value of about pH 7. It was very effective on seedling growth to apply 1 Kg of nutrient material per 110 1 of bed soil as basic dressing because of containing not only quick-acting nitrate but also slow-releasing organo-nitrogen and sufficient potassium.

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Distributions of Urea and Urea Decomposition Rates in an Estuarine System of Mankyung and Dongjin Rivers, Korea (만경.동진강 하구계에서 요소와 요소 분해속도의 분포)

  • 심재형;조병철
    • 한국해양학회지
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    • v.29 no.4
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    • pp.402-413
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    • 1994
  • To understand the significance of urea in the pool size of nitrogenous nutrients and in nitrogen (N) requirements of primary producers in and estuarine system of Mankyung and Dongjin rivers nutrients, and urea decomposition rates between June 1992 and February 1994. Urea concentration during the study period ranged from undetectable to 12.5 ug-at. N 1/SUP -1/, contributing to 0-96% (mean of 11%) of the total nitrogenous nutrients in the study area. Urea comprised a major component of the total nitrogenous nutrients when concentration of total nitrogenous nutrients was low. Urea decomposition rates in the water column ranged from 0.02 to 5.77 nM h/SUP -1/, and netplankton was the major decomposer of urea. Vertical distributions of urea decomposition rates in the water column showed generally small variabilities (i.e. < 3 fold). The decomposition rates of urea in the MD estuary would supply 0.2 ∼88.4% of phytoplankton N requirements. The major contribution of urea to phytoplankton N requirements was found when phytoplankton production was low (<50 mg C m/SUP -2/d/SUP -1/).

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Process Development of Algae Culture for Livestock Wastewater Treatment Using Fiber-Optic Photobioreactor (축산폐수 처리를 위한 광섬유 생물반응기를 이용한 조류 배양 공정 개발)

  • 최정우;김영기;류재홍;이우창;이원홍;한징택
    • KSBB Journal
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    • v.15 no.1
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    • pp.14-21
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    • 2000
  • In this study, algae cultivation using the photobioreactor has been applied to remove the nitrogen and phosphorus compounds in the wastewater of the livestock industry. The optimal ratio of nitrate and ortho-phosphate concentration was found for the enhancement of removal efficiency. To achieve the high density culture of algae, the photobioreactor consisted of optical fibers wes developed to get the sufficient light intensity. The light could be illuminated uniformly from light source to the entire reactor by the optical fibers. The structured kinetic model was proposed to describe the growth rate, consumption rate of nitrates and ortho-phosphates in algae culture. The self-organizing fuzzy logic controller incorporated with genetic algorithm was constructed to control the semi-continuous wastewater treatment system. The proposed fuzzy logic controller was applied to maintain the nitrated concentration at the given set-point with the control of wastewater feeding rate. The experimental results showed that the self-organizing fuzzy logic controller could keep the nitrate concentration and enhance algae growth.

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Sequencing batch reactor treating ship sewage and external carbon source (연속 회분식 공정을 이용한 선박오수와 외부탄소원의 혼합처리)

  • Park Sang-Ho;Choi Jeong-Hye;Ko Sung-Chul;Kim In-Soo
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2004.11a
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    • pp.147-152
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    • 2004
  • In Sequence Batch Reactor (SBR), the removal efficiencies if nutrient materials such as nitrogen and phosphate depend highly on quantity and quality of organic carbon source. Food waste thai contains abundant organic materials has been produced in ship. The applicability if anaerobically fermented if food waste (AFFW) as an external carbon source was examined in the lab-scale SBR process operated at $25^{\circ}C$. With the addition if AFFW increased, average removal efficiencies if $COD_cr$, T-N, T-P changed to $98.5\%,\;95\%,\;93\%$, respectively. Denitrification rate is 0.30g $NO_3-N/g\;VSS{\cdot}day$. In summary, it was suggested tint AFFW sould be used as an economical and effective carbon source for the biological nitrogen and phosphate removal.

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