• Korean Journal of Soil Science and Fertilizer
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• v.18 no.4
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• pp.413-418
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• 1985

Tobacco plant(8-leaf seedlings) were grown on water culture fertilized with different N sources ($NO_3-N$, $NH_4-N$, $NO_3+NH_4-N$) during 15 days. Daily uptake of nutrients and inorganic constituents in plants were investigated in relation to growth responses of them. 1. Nitrate-fed plant showed higher daily uptake of inorganic cations than those in other treatments, and reached about two times higher uptake of nitrogen and three times more uptake of cations (K, Ca, Mg). Potassium was preferentially uptaken at a very fast rate from the beginning after treatment. Also $NO_3-N$ tended to be taken up selectively by the plant from the mixture of nitrate and $NH_4-N$. 2. The initial pH (pH 6.0) of culture medium drastically changed into acid (pH 4.0) in the $NH_4-N$ medium, but into slightly higher (pH 6.4) in the nitrate when measured after exposure of 24 hours. The mixture also tended to show an acidity but much weaker than $NH_4-N$ solution. 3. Nitrate-fed plant had a normal growth pattern but $NH_4-N$ fed plant almost stopped growing. Those plants containing both nitrate and ammonium N were also showed very poor growth.

• KSBB Journal
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• v.14 no.2
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• pp.174-180
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• 1999

An actinomycetes strain No.4 which produce the cholesterol oxidase(EC 1.1.3.6), was isolated from soil and identified as Streptomyces sp. based on taxonomic studies. The conditions of cholesterol oxidase production and enzymatic properties were investigated. The optimum composition of medium for production of the enzyme was 1% soluble starch, 2% corn steep liquor, 0.1% $KH_2PO_4$, 0.1% $NaNO_3$ and 0.05% $MgSO_4$ (pH 7.0). The optimum pH and temperature of the cholesterol oxidase were pH 6.0~7.5 and $37^{\circ}C$, respectively. The enzyme was stable in the range of pH 6.0~9.0. The isoelectric point determined by multichambered electrofocusing unit was in the range of pH 6.0~6.5.

• Applied Biological Chemistry
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• v.43 no.2
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• pp.124-129
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• 2000

Nitrogen and P in surface runoff and eroded sediment from cropland areas can contaminate streams and lakes. Runoff losses of N and P were determined in a small field plot $(14.3{\times}24.8\;m)$ of peach orchard from March to November in 1999. Nitrogen and P were applied in the rate of 172 and 46 kg/ha using chemical fertilizer and mixed oil cake fertilizer. During the season, in 26 rainfall events, $421.5\;m^3/ha$ of runoff including 1,989 kg/ha of soil loss was collected. Concentrations of total-N, $NO_3-N$, $NH_4-N$, total-P and $PO_4-P$ in runoff samples were in the range of $4.7{\sim}171.0,\;0.1{\sim}188.0,\;0.13{\sim}3.36$, $0.58{\sim}4.99$ and $0.05{\sim}3.71\;mg/l$, respectively. Total loss of N was 16.39 kg/ha and 75% of the loss was $NO_3-N$. Total loss of P was 1.04 kg/ha, and $PO_4-P$ and sediment bound P accounted for 47 and 27% of the total loss, respectively. The losses of N and P were about 9.5 and 2.3% of the applied N and P in the plot, respectively. Although the loss of N or P would be relatively small in agricultural aspect, considering the high concentrations of N and P in runoff, loss of N and P from croplands should be controlled to reduce the eutrophication problem of stream waters.

• Proceedings of the KSRS Conference
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• 2007.03a
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• pp.43-48
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• 2007

본 연구는 벼 군락의 분광반사율 지표를 측정할 수 있는 인공광원을 사용하는 능동형 광학 센서인 GNDVI 를 활용하여 생육시기별 식생지수와 엽 질소함량과의 관계를 구명하여 벼 군락의 엽 질소함량을 추정하고자 하였다. 농업과학기술원 답작 포장에서 공시품 종인 추청벼를 이용하여 난괴법 3반복으로 시험구 배치를 하고 질소 4수준 3반복으로 실험을 수행하였다. 벼 생육시기별 GNDVI 와 엽 질소함량과의 관계를 2년 간 (2005, 2006)의 자료를 통하여 분석해 보았다. 2005년의 경우 벼 생육시기동안 9시기의 GNDVI값과 그 당시 시료를 샘플링하여 분석한 엽 질소함량과의 관계를 벼 출수전과 출수후로 구분하여 분석해 본 결과 GNDVI 값은 출수전 ($r=0.78^{***}$ n=60) 보다 출수후 ($r=0.89^{***}$, n=59) 가 엽 질소함량과의 상관계수가 높았다. 2006년은 20시기동 안 생육시기별 식생지수와 엽 질소함량과의 상관 분석한 결과 착근기 (6월 5일) $r=0.84^{***}$, 유수분화기 (7월 11일) $r=0.95^{***}$, 출수기 (8 월 16일) $r=0.87^{***}$, 수확기 (10월 13일) $r=0.90^{***}$ 으로 출수전의 경우 7월 11일이 상관계수가 가장 높았고， 이 결과는 2005년 동일시기 (7월 11일) 식생지수와 벼 엽 질소함량과의 상관계수가 가장 높았던 ($r=0.91^{***}$) 것과 일치하였다. 벼 생육시기 변화에 따른 식생지수와 엽 질소 흡수량과의 관계를 살펴보았는데 벼 출수전의 경우 GNDVI는 7월 11일 에 엽 질소 흡수량과의 상관계수 ($r=93^{***}$)가 가장 높은 결과를 보였고 출수후의 경우에는 시기에 따라 상관계수가 고르게 높게 나타났다. 엽 질소함량과의 상관관계가 높았던 2005년，2006년 7월 11일 식생지수 데이터를 함께 이용하여 엽 질소함량과의 관계를 추정식으로 작성하였다. GNDVI를 이용하여 2005년과 2006년 실측한 엽 질소함량 값과 추정 값을 비교해 본 결과 2005년과 2006년의 결정계수가 각각 0.88, 0.94로 2006년이 더 예측률이 높게 나타났다. GNDVI 값을 이용하여 엽 질소함량 추정값과 실측값을 비교해 본 결과 결정계수가 0.86으로 추정값과 실측값이 근접하게 분포하였다.

• The Korean Journal of Mycology
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• v.35 no.2
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• pp.76-80
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• 2007

This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and investigated its optimal culture condition. Among four kinds of mushroom culture media, PDA medium was selected as the suitable culture medium. The optimal conditions for the mycelial growth of G. frondosa in PDA medium were $25^{\circ}C$ and $4{\sim}5$ of pH, respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively, and the highest mycelial growth was observed when the C/N ratio was $10{\sim}20$.

• Applied Biological Chemistry
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• v.17 no.2
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• pp.93-116
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• 1974

A potent intracellular-lipid-producing yeast, Rhodotorula glutinis var. glutinis SW-17, was screened out from a variety of arable soils, compost heaps, and fodders, and two strains of excellent extracellular-lipid-producing yeasts, Rhodotorula glutinis var. glutinis SW-5 and Rhodotorula graminis SW-54, were screened out from the surface of many species of leaves. And then the intra- and extra-cellular lipid productions by those Rhodotorula yeasts were studied. The results were as follows: 1. During the shaking culture of 8 days at $24^{\circ}C$, both the intra- and extra-cellular lipid accumulation started almost at the stationary phase of growth, when the nitrogen source in the medium was a little more than half used up. The intracellular lipid production by Rhodotorula glutinis var. glutinis SW-17 reached 58.42% (w/w) of dried yeast, and the extracellular lipid production by Rhodotorula graminis SW-54 amounted to 2.62g per liter of the medium. 2. After the carbon and nitrogen sources in the medium were almost consumed, if the yeasts were shake-cultured further in a state of starvation, the yeast cells re-utilized the already produced intra- and extra-cellular lipids and the lipids completely disappeared in the medium in about 90 days. 3. The relative concentration of carbon and nitrogen sources in the media greatly influenced both the intra- and extra-cellular lipid production. When the nitrogen source in the medium was almost used up for the growth of yeast, and excess carbon sources were still available, the lipid production vigorously proceeded. As long as the nitrogen source concentration in the medium was high, the lipid production was greatly suppressed. 4. The optimum pH for both the intra- and extra-cellular lipid production by those yeasts was pH 5.0-6.0. 5. The fatty acid components of the intracellular lipid of Rhodotorula glutinis var. glutinis SW-17 were myristic, palmitic, palmitoleic, stearic, oleic, linoleic, and linolenic acids. The largest components of the fatty acids were palmitic acid equivalent to 30-45% of the whole fatty acids and oleic acid equivalent to 35-50%. 6. The fatty acid components of the extracellular lipid of Rhodotorula glutinis var. glutinis SW-5 and Rhodotorula graminis SW-54 were myristic, palmitic, stearic, oleic, linoleic, linolenic, 3-D-hydroxypalmitic, and 3-D-hydroxystearic acids. The largest components of the fatty acids were 3-D-hydroxypalmitic acid equivalent to 22-25% of the acids and 3-D-hydroxystearic acid equivalent to 13-17%. 7. The polyol component of the intracellular lipids was only glycerol, whereas the polyols of extracellular lipids were glycerol, mannitol, xylitol and arabitol.

• Journal of the Korea Organic Resources Recycling Association
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• v.15 no.1
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• pp.149-158
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• 2007

To investigate the characteristics of the nitrogen mineralization of oil cakes according to changes in temperature(10, 20, $30^{\circ}C$) moisture(40, 50, 60, 70% of field capacity in loam and 50, 60, 70, 80% of field capacity in sandy loam), mineral nitrogen was measured in soil after incubation for 30 days. In addition, the mineralization in sandy loam and loam which had different soil texture were compared. According to incubating the castor seed, soybean, and rice bran cakes with soil, the higher the temperature and moisture content were, the higher the content of mineral nitrogen was observed. The content of mineral nitrogen was higher in sandy loam than loam. The content of mineral nitrogen was decreased with soil depth increasing and was also higher in sandy loam than loam.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.4
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• pp.368-371
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• 1983

Energy rich rice straw was subjected to biological processes involving the transformation of added nitrogen. A part of soil ammonium nitrogen was steadily exhausted when energy rich rice straw was decomposed. More vigorous transformation of added nitrogen ocurred during the first 5 days of incubation period than after 10 days of incubation period. Furthermore, transformation of added nitrogen occurred more markedly when more rice straw and less nitrogen were added. Remineralization of immobilized nitrogen did not take place in this experiment with 50 days of incubation.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.249-255
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• 2023

Korean melon (Cucumis melo L.) is grown mostly in Northeast Asia area, and as a fruit mainly produced in Korea, the yield per unit area continues to improve, but the cultivation method is limited to soil cultivation, so it is necessary to develop hydroponic cultivation technology for scale and labor-saving is needed. As the ratio of NO₃^- increased, the plant height, the leaf length, the leaf width, and the internode length became longer and larger. On the other hand, the SPAD value decreased. The lower the ratio of NO₃^-, the faster the female flower bloom, and there was no difference in fruit maturity between treatments. There was no difference in the shape of fruit according to the ratio of NO₃^-, and the hardness was higher as the ratio of NO₃^- was lower. The total yield from March to July was KM3 5,650 kg/10a and KM1 4,439 kg/10a, 27% higher in KM3 and, in particular, 36% higher in quantity from March to May, when Korean melon prices were high season. Therefore, it was judged that it would be appropriate to supply NO₃^- suitable for hydroponic cultivation of Korean melon, which was formalized in December and produced from spring, at the level of 6.5 to 10 me·L^-1.

• Journal of Mushroom
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• v.3 no.2
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• pp.75-78
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• 2005

The results of studies for determining the nutrients sources of mycelial growth for optimal condition of Pleurotus cornucopiae are as follows; 1) Optimal carbon source of mycelial growth of Pleurotus cornucopiae is maltose(357mg/25ml/15d); 2) Optimal nitrogen source of mycelial growth of Pleurotus cornucopiae is peptone(347mg/25ml/15d); 3) Optimal organic acid source of mycelial growth of Pleurotus cornucopiae is glutamic acid(389mg/25ml/15d); 4) Optimal vitamin source of mycelial growth of Pleurotus cornucopiae is biotin(399mg/15d); and 5) Optimal C/N ratio for mycelial growth for determining the condition of Pleurotus cornucopiae is 40(398mg/15d).