• Journal of Mushroom
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• v.12 no.1
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• pp.35-40
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• 2014

Several bacteria are known as the causal agents of diseases of the cultivated button mushroom(Agaricus bisporus) and oyster mushroom(Pleurotus ostreatus). Pseudomonas tolaasii is the causal agent of brown blotch disease of commercial mushrooms. Pseudomonas sp. HC1 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This can markedly reduce the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Pseudomonas sp. HC1, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 5.0 and $20^{\circ}C$, respectively. The optimal culture medium for the growth of tolaasin inhibitor bacterium was determined as follows: 0.9% dextrin, 1.5% yest extract, 0.5% $(NH_4)_2HPO_4$, 4mM $FeCl_3$, and 3.0% cysteine.

• Korean Journal of Microbiology
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• v.40 no.1
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• pp.37-42
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• 2004

To differentiate commercial bifidobacteia for Bifidobacterium lactis Bb-12, Bif. longum Bb-536 and Bif. infantis Maeil-K9, we studied the various carbon source, the nitrogen sources and antibiotics. Amygdalin and fructose were good candidates for carbon sources, and tryptone was suitable for nitrogen sources to design a new selective media for three commercial bifidobacteria. In the case of the amygdalin-containing medium as carbon sources, Bif. lactis Bb-12 and Bif. infantis Maeil-K9 showed good growth, and in fructose-containing medium, Bif. longum Bb-536 showed good growth. In antibiotics resistance study, the addition of 1 mg/L doxycyclin was very effective for differentiation of each bifidobacteria. Doxycyclin did not affect the growth of Bif. lactis Bb-12 and Bif. infantis Maeil-K9, but Bif. longum Bb-536 was completely inhibited by doxycyclin. Finally to confirm the selection capability of newly designed selective media, temperature-shocked bifidobacteria were cultured on them. As the results, fructose or doxycyclin containing medium showed for high growth for temperature-shocked bifidobacteria, but amygdalin containing medium showed low growth of temperature-shocked bifidobacteria.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.434-437
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• 2004

본 반층벽체 적용지역의 경우, 침출수의 영향으로 암모니아성 질소의 농도가 최대 29.12mg/L까지 검출되고 있으며 반응물질은 암모니아성 질소제거에 적합한 제올라이트를 선정하였다. 투수성 및 다짐강도를 고려하여 왕사와 50:50의 비율로 혼합, 적용하였으며 지하수 평균유속은 0.0864m/d이고 평균지하수위는 원지반 기준 - 4.0m의 분포를 나타내었다. 지질조사 결과, 적용구간의 지질구조는 매립층, 풍화토, 풍차암, 연암의 순서로 분포하고 있으며, 반응벽체의 설계깊이는 현장 시추조사 결과를 바탕으로 지하수의 Under-pass를 방지하기 위하여 연암층 50cm까지 관입시키도록 설계하였다. 최종적으로 선정된 반응벽체의 규모는 35m(길이) $\times$ 1.2m(두께) $\times$ 8.5 ～ 9.42m(구간별 깊이)로 설계에 반영되었다. 현재, 지하수감시정을 이용한 모니터링 결과, 상부지하수 감시정에서의 오염원은 우기의 영향으로 간헐적으로 발생하고 있으나 하부 지하수 감시정에서의 암모니아성 질소농도는 불검출 되거나 0.5 mg/L 이하로 유지되고 있다.

• Journal of the Korean Vacuum Society
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• v.4 no.2
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• pp.183-188
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• 1995

고에너지(50-200KeV)로 가속된 이온을 모재표면에 물리적으로 투입하므로써 표면의 조성 및 조직을 변화시키는 공정인 이온주입기술을 이용하여 경량고강도소재로 각광받고 있는 AI2218 합금의 재식성 향상을 연구하였다. 질소이온주입은 DuoPIGatron 이온원을 사용하여 가속전압 100KeV, 조사량 $1{\times}10^{17}ions/\textrm{cm}^2$~$5{\times}10^{17}ions/\textrm{cm}^2$의 조건으로 행하였으며 AI합금의 열화를 방지하기 위하여 시편온도를 $60^{\circ}C$이하로 유지하였다. 질소이온 주입재의 재식성 평가를 위하여 3.5% NaCI 용액에서 양극분극시험 및 5% NaCI 용액에서 염수분무시험을 행하였다. Auger Electron Spectroscopy와 Transmission Electron Microscopy을 이용하여 표면의 질화물형성 여부를 조사하였으며, Scanning Electron Microscopy을 이용하여 부식된 표면을 관찰하였다. AI2218합금에 질소이온을 주입한 결과 표면에 미세한 AIN 석출물을 형성하였으며 이러한 질화물형성에 의해 공식(pitting)발생을 억제하고 부식전류밀도를 감소시켜 내식성이 향상되었다.

• Journal of Plant Biology
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• v.34 no.1
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• pp.31-35
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• 1991

Induction of symbiosis between N. muscorum and cultured tobacco cells associately cultured on nitrogen-free medium and effects of polyamines on culture condition were carried out. Analysis of amino acid composition in associate cultures showed increase in total amino acid amounts than single culture of cultured tobacco cells and methionine was markly increased in associative culture on N-free medium treated with 1 mM spermine. These results indicated that the composition of amino acids increased effectively in associative cultures by nitrogen fixation of N. muscorum.scorum.

• KSBB Journal
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• v.14 no.2
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• pp.181-187
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• 1999

We have investigated industrial media for lactic acid fermentation to reduce the cost of nitrogen sources. Corn steep liquor (CSL) was successfully used at 5% (v/v) in batch fermentations. Use of soluble CSL improved the productivity about 20% with an advantage of clearer fermentation broth. Yeast extract-complemented CSL improved the productivity about 20% with an advantage of clearer fermentation broth. Yeast extract-complemented CSL media further increased the increased the productivity. It was found that 3.1 g/L yeast extract and 5% CSL could be an effective substitute for 15 g/L yeast extract in 10% glucose medium. Brewing yeast was also used as a sole nitrogen source equivalent to 5% CSL. A continuous culture coupled with cell-recycle by microfiltration at the dilution rate of 0.05-0.065 h-1 led to the highest lactic acid productivity. Lactic acid was recovered by electrodialysis from the cell free broth. Depleted cell free broth supplemented with 5-10 g/L of yeast extract performed reasonably in batch and continuous cultures. Reuse of the fermentation broth may reduce the cost of raw materials as well as minimize the fermentation wastes.

• Journal of Korean Society of Water and Wastewater
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• v.26 no.6
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• pp.833-840
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• 2012

This study aimed to investigate growth rate and nutrient removal efficiency of Chlorella vulgaris according to nitrogen sources and frequency of pH adjustment. Nitrogen and phosphorus removal efficiencies were evaluated in the three different conditions using $NO_3{^-}$, $NH_4{^+}$ as a sole nitrogen source and mixed condition. Initial nutrient concentrations in artificial wastewater were 30 mg-N/L and 3 mg-P/L similar to secondary wastewater effluent. When nitrogen source was $NO_3{^-}$, there was no inhibition on the growth of C. vulgaris with adjusting pH every 24 hr while growth inhibition occurred with $NH_4{^+}$ caused by pH drop. N, P removal efficiencies were no significant depending on the nitrogen sources. As pH was adjusted to 7 by pH-stat, growth rate and nutrient removal efficiencies were increased compared to adjusting pH every 24 hr, however, growth rate and nutrient removal efficiencies were no significant depending on the nitrogen sources.

• Economic and Environmental Geology
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• v.55 no.5
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• pp.439-445
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• 2022

Organic pollutants that contained in stream sediments have origins of mountain soil in natural and cattle manure in human activity. Nitrogen and carbon isotope analysis for mountain soil, cattle manure and stream sediment were performed for contribution evaluation of organic pollutants in Toil stream of Yeongju dam basin. Average carbon isotope ratio(δ¹³C) is -25.17‰, -22.34‰, and -26.39‰ for river sediments, cattle manure and mountain soil, respectively. Result of carbon isotope analysis suggests that river sediments are more affected by acid soils. Average value of the nitrogen isotope ratio (δ¹⁵N) is 9.46% for river sediment, 1.99% for mountain soil, and 19.53% for cattle manure. Result of nitrogen isotopic analysis show that contribution of cattle mature is slightly higher than that of mountain soil in Toil stream sediments.

• Environmental engineer
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• v.23 s.240
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• pp.30-35
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• 2006

• Bulletin of Korea Environmental Preservation Association
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• v.24 no.5_6
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• pp.51-56
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• 2002